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(1981)
Insulin Degradation by Human Erythrocyte Lysates Shriniwas G. Nerurkar and Kanwal K. Gambhir1 In vitro hemolysates
of isolated human erythrocytes
de-
grade 125l-labeled insulin. Ten- to 100-fold dilutions of the hemolysate give a proportionately decreased degradation of 125l-labeled insulin at 37 #{176}C, while dilutions of up to
Materials and Methods Monocomponent
insulin (lot no. JM95AF)
was purchased
from Elanco Laboratories, Indianapolis, IN 46206 for iodination with Na12I (by New England Nuclear). “Pentex” bovine
eightfold do not. Like the control, diluted “Buffer G” containing 5 mmol/L Tris and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer alone, more than 500-fold dilutions of the hemolysate or boiled hemolysate (in buffer) caused negligible (
