Iron & BMPR-2 : friend or foe?

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+LPS. BMPR2 wt m wt m wt m wt m. +Anti-IL6. pSTAT3. Tubulin. +LPS. STAT3. pSTAT3. Tubulin. +/- bmpr2. +LPS. +/+. +LPS. STAT3. 0. 20. 40. 60. 80. 100. NS.
bmpr2+/+

Fold change in IL6

100

10

*

B

bmpr2+/-

NS

1

0.1

***

100

Fold change in KC (mouse analogue of IL-8)

A

bmpr2+/+ bmpr2+/-

10

NS

1

0.1

Baseline

LPS 24h

Baseline

LPS 24h

* ** **

100000

bmpr2+/+ bmpr2+/-

D

10000

1000

100

KC, mouse analogue of IL-8 (pg/ml)

IL-6 (pg/ml)

C

** 50000

30000 20000 10000 0

BMPR2 pStat3

Total Stat3 Tubulin

+/+

+/-

Baseline

LPS 24h +LPS

E

+/+

+/-

bmpr2+/+

40000

LPS 3h

*

F pStat3/ total Stat3

LPS 3h

*

bmpr2+/-

10 Baseline

**

1.5

bmpr2+/+

* *

1.0

NS

0.5

0.0 Baseline

LPS 24h

LPS 24h

bmpr2+/-

B

bmpr2+/+

*

20 15 10 5

*

0 LPS 1

0.1%

*

5

LPS 1

BMPR2wt BMPR2mut

80 NS

50 0

Tubulin

bmpr2

m

wt

m

1.5

wt

m

wt

m

+/-

pSTAT3

40

STAT3

20

Tubulin LPS 10 BMPR-2 wt

BMPR-2 wt

I

BMPR-2 mut

*

BMPR-2 mut

Densitometry (pSTAT3/tubulin)

wt

+/+

+LPS

NS

0.1%

+LPS

LPS 10

+LPS

*

H +LPS

STAT3

0.1%

F

+Anti-IL6

pSTAT3

0

60

LPS 10

G

*

50

0 0.1%

BMPR2

100

LPS 20

LPS 10

100

150

*

0

E *

100

150

*

0.1%

bmpr2+/+ bmpr2+/200

*

10

LPS 20

IL-6 (pg/ml)

KC, mouse analogue of IL-8 (pg/ml)

D

LPS 10

bmpr2+/-

15

bmpr2+/+ bmpr2+/-

C

200

*

*

*

% increase in cell number from baseline

Fold change in IL-6

Fold change in KC (mouse analogue of IL-8)

bmpr2+/-

25

bmpr2+/+

IL-6 (pg/ml)

A

1.0

0.5

0.0 0.1%

LPS

Anti-IL6

Anti-IL6 +LPS

150

* NS

100 50 0 10% FBS

IL6

IL8

IL6 + IL8

A

B

Count/cell

BMPR2wt BMPR2mut

**

30000

BMPR2wt 0.1%

BMPR2mut 0.1%

Count/cell

20000

Count/cell

5000

Count/cell

C

p