Isolation and Characterization of a Novel SOMATIC ...

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Xia Li & Yu-Han Fang & Jing-Dan Han & Shu-Nong Bai &. Guang-Yuan Rao. Published online: 14 August 2014. © Springer Science+Business Media New York ...

Plant Mol Biol Rep (2015) 33:638–647 DOI 10.1007/s11105-014-0769-2

ORIGINAL PAPER

Isolation and Characterization of a Novel SOMATIC EMBRYOGENESIS RECEPTOR KINASE Gene Expressed in the Fern Adiantum capillus-veneris During Shoot Regeneration In Vitro Xia Li & Yu-Han Fang & Jing-Dan Han & Shu-Nong Bai & Guang-Yuan Rao

Published online: 14 August 2014 # Springer Science+Business Media New York 2014

Abstract SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) genes are expressed primarily in seed plants and are involved in the development of embryos from alreadydifferentiated plant cells. This study provides the first evidence for the existence of a SERK gene, designated AcvSERK1, in a fern, Adiantum capillus-veneris. AcvSERK1 belongs to a small family of receptor-like kinases (RLKs), and its sequence and conserved structure closely resemble SERK homologs in other land plants. Phylogenetic analysis of the SERK gene family showed that AcvSERK1 forms a clade with SERK-like genes from non-seed plants and is a member of the SERK1/2 genes group, involved in somatic embryogenesis. Moreover, our analyses reveal that AcvSERK1 is expressed throughout the entire process of embryo development, but its levels decrease with the formation of shoots and seedlings. Additionally, in situ hybridization showed that AcvSERK1 is expressed during green globular body (GGB)-derived somatic embryogenesis and in meristematic calluses. Our results indicate that the AcvSERK1 gene is a good marker for differentiated cells that are capable of forming embryos in A. capillus-veneris, suggesting that SERK genes have played an important role in embryogenesis during the evolution of land plants.

Keywords Adiantum capillus-veneris . Gene expression . AcvSERK1 gene . Shoot regeneration . Homolog Electronic supplementary material The online version of this article (doi:10.1007/s11105-014-0769-2) contains supplementary material, which is available to authorized users. X. Li : Y.

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