Isolation and characterization of halophilic Bacillus sp. BS3 able to ...

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Nov 20, 2013 - Document heading doi: Isolation and characterization of halophilic Bacillus sp. BS3 able to produce pharmacologically important biosurfactants.
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Asian Pacific Journal of Tropical Medicine (2013)876-883

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Asian Pacific Journal of Tropical Medicine journal homepage:www.elsevier.com/locate/apjtm

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Isolation and characterization of halophilic Bacillus sp. BS3 able to produce pharmacologically important biosurfactants MBS Donio, SFA Ronica, V Thanga Viji, S Velmurugan, J Adlin Jenifer, M Michaelbabu, T Citarasu* Centre for Marine Science and Technology, Manonmaniam Sundaranar University Rajakkamangalam, Kanyakumari District, Tamilnadu, India 629502

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ABSTRACT

Article history: Received 10 August 2013 Received in revised form 15 September 2013 Accepted 15 October 2013 Available online 20 November 2013

Objective: To characterize the pharmacological importance of biosurfactants isolated from halophilic Bacillus sp BS3. Methods: Halophilic Bacillus sp. BS3 was isolated from solar salt works, identified by 16S rRNA sequencing and was used for screening their biosurfactant production. Characters of the biosurfactant and their anticancer activity were analyzed and performed in mammary epithelial carcinoma cell at different concentrations. Results: The biosurfactant were characterized by TLC, FTIR and GC-MS analysis and identified as lipopeptide type. GC-MS analysis revealed that, the biosurfactant had various compounds including 13Docosenamide, (Z); Mannosamine, 9- and N,N,N',N'-tetramethyl. Surprisingly the antiviral activity was found against shrimp white spot syndrome virus (WSSV) by suppressing the viral replication and significantly raised shrimp survival (P