Curriculum Vita. James E. Koltes. Work address: Attie Laboratory. Department of
Biochemistry. University of Wisconsin-Madison. 433 Babcock Drive. Madison ...
Curriculum Vita
James E. Koltes Work address: Attie Laboratory Department of Biochemistry University of Wisconsin-Madison 433 Babcock Drive Madison, WI 53706-1544, USA
Post Doctoral Experience (3/09 – present) Research goal: To find and characterize novel genes and gene networks that cause susceptibility or resistance to type II diabetes mellitus. Approach: The Attie lab works as a team with statisticians and bioinformaticians to develop systems biology approaches that direct genetic and biochemical laboratory research.
Fulbright fellow (1/08 – 12/08) Research focus: transgenesis of male-specific muscular hypertrophy (myostatin transinactivation) in the mouse at University of Liege, Belgium.
Education Iowa State University (8/02 –12/07) Ph.D degree program in Genetics, minor in Statistics (GPA: 3.53/4.0) Thesis title: The role of PRKG2 in Angus dwarfism Advisor: Dr. James M. Reecy University of Wisconsin-Madison (9/97 –12/01) BS degree in Natural Sciences with honors (College of Agric. and Life Sciences) Majors: Dairy Science and Genetics (GPA: 3.4/4.0)
Publications Referred papers Alexander, L.J., L.A. Kuehn, T.L.P. Smith, L. Matukumalli, B. Mote, J.E. Koltes, J. Reecy, T.W. Geary, and M.D. MacNeil. A Limousin specific myostatin allele affects longissimus muscle area and fatty acid profiles in a Wagyu-Limousin F2 population. J. Animal Sci. 2009, Feb 11 [Epub ahead of print]. Koltes, J.E., Z-L. Hu, E. Fritz, and J.M. Reecy. BEAP: BLAST extension and alignment program- a tool for contig construction and analysis of preliminary genome sequence. BMC Res. Notes. 2009, 2:11. Tshipuliso, N.O.M, L.J. Alexander, T.W. Geary, W.M. Snelling, D.C. Rule, J.E. Koltes, B.E. Mote, and M.D. MacNeil. Mapping QTL for fatty acid composition that
James Koltes segregate between Japaneese Black and Limousin breeds. South African Journal of Animal Science. 2008. 38(2): 126-130. Koltes, J.E., B.P. Mishra, D. Kumar, R.S. Kataria, L.R. Totir, R.L. Fernando, R. Cobbold, D. Steffen, W. Coppieters, M. Georges, and J.M. Reecy. A Nonsense mutation in the Kinase domain of cGMP-dependant, type II protein kinase (PRKG2) is the causative mutation for Angus dwarfism. In submission to PNAS. Papers in Process Koltes, J.E., V.L. Cooper, and J.M. Reecy. Transcriptional profiling of PRKG2-null growth plate implicates TP53 as a possible down-stream target of PRKG2. To be submitted to Physiological Genomics. Abstracts Koltes, J.E. and J.M. Reecy. 2006. Functional analysis of a PRKG2 nonsense mutation in American Angus dwarf cattle. Proc. Of 30th International Society of Animal Genetics. (C476) p79. Puerto Seguro, Bahia, Brazil. Grant, M.S., J.E. Koltes, and J.M. Reecy. 2006. The Effects of Myostatin on Skeletal Muscle Re-Growth Following Disuse Atrophy in Mice. J. Anim. Sci. 84 (Suppl. 2): p53. Koltes, J.E., L.R. Totir, R.L. Fernando, and J.M. Reecy. 2006. Identification of a putative causal mutation for dwarfism in PRKG2. Plant and Animal Genome meeting XIII proceedings. Proc of Plant and Anim. Genome XIV. (P527) p233. San Diego, CA. Koltes, J., R. Totir, R. Fernando, and J. Reecy. 2005. Fine-mapping of a 2.8cM region associated with dwarfism in American Angus cattle. Proc. Of the 3rd symposium of Genet of Anim. Health (P24). Ames, IA. Koltes, J., Z-L Hu, and J. Reecy. 2005. Localized development of a high resolution sequence comparative map of bovine chromosome 6. Proc of Plant and Anim. Genome XIII. (P523) p201. San Diego, CA. Mishra, B., J. Koltes, R. Totir, R. Fernando, J. Cavanagh, M. Georges, W. Coppieters, and J. Reecy. 2004. Mapping of the gene causing disproportionate dwarfism in American Angus cattle. Proc. of the International Society of Animal Genetics. W003. Tokyo, Japan Berger, P. J., J. Koltes, M. H. Healey, M. S. Ashwell, R. D. Shanks, H. Schlesser, and H. A. Lewin. 2003. Putative quantitative trait loci affecting perinatal survival in eleven Holstein families. J. Dairy Sci. 86(Suppl. 1) : p161. Koltes, J., P. J. Berger, J. C. M. Dekkers, M. H. Healey, M. S. Ashwell, R. D. Shanks, H. Schlesser, and H. A. Lewin. 2003. Evidence for major quantitative trait loci 2
James Koltes affecting perinatal survival in two elite Holstein sire families. Proc. The John M. Airy Beef Cattle Symposium. Visions for genetics and breeding. p131. Ed. J. M. Reecy. Johnston, IA. Research Reports Koltes, J.E., B.P. Mishra, L.R. Totir, R.L. Fernando, W. Coppieters, M. Georges, and J.M. Reecy. 2006. Angus dwarfism: the short story about ISU’s discovery of a causal mutation for dwarfism in American Angus. Iowa State University Animal Science leaflet. Koltes, J.E. and J.M. Reecy. 2006. NRSP-8 update of Iowa State University’s molecular genetic research in beef cattle, highlighting the discovery of a putative causal mutation for Angus dwarfism. USDA NRSP-8 Research report. San Diego, CA.
Patents GENETIC TEST FOR THE IDENTIFICATION OF DWARFISM IN CATTLE – USPTO #11/556,284
Teaching Experience Iowa State University • Lecturer: Animal Science 345 Animal growth and development along with Dr. Reecy, 2007; guest lecturer, 2006. • Guest lab instructor: Animal Science 360 Fresh meats: muscle growth and development (2006, 2002) • Mentor: Visiting Scientists/Graduate/Undergraduate/ high school research programs. Mentored 8 students in genetics laboratory research including: Aracely Acevado, Julie Collins, Brandon Cornilieus, Jessica Frerichs, Andrea Moe, Nicole Rutscher, Kathy Parton DVM, and Susan Piripi (2004 – 2007) • Teaching assistant: Animal Science 352 Livestock improvement through animal breeding (2004) University of Wisconsin-Madison • Teaching assistant: Farm and Industry Short Course Genetic Improvement of Dairy cattle (2001) • Guest lecturer: Dairy Science 272 Pre-capstone seminar: Internship experience in animal genetics (2000)
Honors and Awards • • • • • •
Fulbright Fellowship (Belgium ‘08) Belgian-American Educational Foundation Research Fellowship ‘08 Iowa State University Sports Club Council Advisor of the year (ISU fishing club) ’06-‘07 Miller Fellowship ’03-‘07 Bioinformatics travel grant to Plant & Animal Genome conference ‘06 John Airy Endowed Graduate Scholarship ’04, ‘05 3
James Koltes • • • •
Ag. & Life Sci. Alum. Assoc. (WALSAA) Active Aggie Senior Activities Award (1 of 2) ‘01 Wisconsin Alumni Association Outstanding Junior Award (1 of 6 university wide) ‘00 Hoards Scholar ’00, ‘01 Schmidt Academic Merit Scholarship; ‘98, ‘99
Professional Development Presentations • Molecular dissection of dwarfism in American Angus. Interdepartmental Genetics Seminar. Iowa State University, Ames. (2005) • Fine-mapping of the genomic region associated with disproportionate dwarfism in Angus cattle. Interdepartmental Genetics Seminar. Iowa State University, Ames. (2004) • Fine-mapping of the locus associated with dwarfism in Angus cattle. Animal Breeding & Genetics Seminar. Iowa State University, Ames. (2004) • Report on the stillbirth QTL mapping project in Holsteins at ISU. NC State S1008 Dairy cattle survival meeting. Raleigh, NC (2003)
Leadership experience ISU Animal Breeding and Genetics Graduate Student Organization (1/03 – 12/04) • President: Responsible for organization of meetings and activities as well as assisting the treasurer in acquiring operating funds through the student government • Vice President: Responsible for assisting the president and organizing club events. • Journal club chair: Organized journal club events, speakers, and logistics. UW-Madison College of Agriculture and Life Sciences Student Council (1/98 – 5/01) • President: In charge of coordinating campus events. First to secure $12,000 in funds for CALS campus student organizations. Strengthened student participation in council and reshaped the image and constitution of the council. • Liaison Officer : Selected students for CALS student faculty committees.
Service to professional societies • • •
Reviewer for Mammalian Genome 2006 Reviewer for Animal Genetics 2006 Assisted in reviewing 2 papers (Journal of Animal Science and Biochemical genetics) and a book chapter on the molecular genetics of beef cattle.
Service to the department (Animal Science) •
4-H Round-up: Assisted Leo Timms with the instruction of dairy cattle management at the ISU dairy (2003). Organized and presented two workshops
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James Koltes
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(beef and swine) on the impact of molecular biology and genomics in livestock improvement (2005 - 2007). Science Bound: Organized and presented a genetics workshop for ABGGSO to high school sophomores at ISU (2005). Academic Quadrathalon: Assisted in organization and supervision the laboratory portion of this event (2004). Academic Knowledge Bowl: Assisted in creating the lab exam as well as organization and supervision for this portion of the event (2004).
Experience in previous laboratory groups • • •
Kirkpatrick Lab. University of Wisconsin-Madison. 2000-2001 Analysis of BMP15 as a candidate gene for twinning rate in cattle. Genetic Visions. Middleton, Wisconsin. Summer 2000 Genotyping and analysis of chromosomes associated with dairy profitability. Dentine Lab. University of Wisconsin-Madison. 1998-1999 Genotyping and analysis of candidate genes for Johne’s and Mastitis resistance in dairy cattle in collaboration with Dr. George Shook.
Extracurricular activities • • • •
Advisor CBAA, competitive collegiate bass fishing governing body (2007-2008) Advisor ISU Bass Anglers fishing team (2006-2007) Volunteer: Hy-Vee kids fishing derby (May 2006) Volunteer: Tri-County big brothers, big sisters kids fishing day (June 2006)
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