Volume 5, Issue 5 (Sep-Oct), 2017 ISSN 0719-4250
Content:
Pages
IFC (Journal of Pharmacy & Pharmacognosy Research).
i
1.- Original article Akram Jamshidzadeh, Yaser Shokri, Nahid Ahmadi, Neda Mohamadi, Fariba Sharififar. Quercus infectoria and Terminalia chebula decrease melanin content and tyrosinase activity in B16/F10 cell lines.
270-277
2.- Original article Moses W. Bariweni, Raymond I. Ozolua. Neuropharmacological effects of the aqueous leaf extract and fractions of Pavetta crassipes (K. Schum) Rubiaceae in mice.
278-287
3.- Original article Nilia De la Paz, Dania Pérez, Mirna Fernández, Dulce M. Soler, Yanet Rodríguez, Antonio Nogueira. Estabilidad física de bases emulsionadas e hidrosolubles con quitosana y acetato de quitosana. [Physical stability of emulsion and hydrophilic gels with chitosan and chitosan acetate].
288-300
4. Original article Claudia M. Plaza, Celina Pérez de Salazar, Marietta Vizcaya, C. Gabriela Rodríguez-Castillo, Gerardo E. Medina-Ramírez, Ramón E. Plaza. Potential antifungal activity of Cladonia aff. Rappii A. Evans.
301-309
5. Original article Gérard A. Toudji, Kossivi Dosseh, Simplice D. Karou, Yao Adjrah, Kokou Anani, Yaovi Ameyapoh, Jacques Simpore. Acute and sub-acute toxicity of Pithecellobium dulce (Roxb.) Benth. stem bark hydroalcoholic extract on Wistar rats.
310-319
6. Original article Janne del C. Rojas, Alexis A. Buitrago, Francisco A. Arvelo, Felipe J. Sojo, Alírica I. Suarez. Cytotoxic activity of different polarity fractions obtained from methanolic extracts of Vismia baccifera and Vismia macrophylla (Hypericaceae) collected in Venezuela.
J Pharm Pharmacogn Res JPPRes
320-326
© 2017 Journal of Pharmacy & Pharmacognosy Research ISSN 0719-4250 http://jppres.com/jppres
JOURNAL OF PHARMACY & PHARMACOGNOSY RESEARCH For specialists working in the pharmaceutical and herbal fields The Journal of Pharmacy & Pharmacognosy Research (JPPRes) is an international, specialized and peer-reviewed open access journal, which publishes studies in the pharmaceutical and herbal fields concerned with the physical, botanical, chemical, biological, toxicological properties and clinical applications of molecular entities, active pharmaceutical ingredients, devices and delivery systems for drugs, vaccines and biologicals, including their design, manufacture, evaluation and marketing. This journal publishes research papers, reviews, commentaries and letters to the editor as well as special issues and review of pre- and postgraduate thesis from pharmacists or professionals involved in Pharmaceutical Sciences or Pharmacognosy. JPPRes has an acceptance rate of 40%. The average time between submission and final decision is 60 days and the average time between acceptance and final publication is 15 days. Manuscripts submitted to JPPRes are only accepted on the understanding that they are subject to editorial review and that they have not been, and will not be, published in whole or in part in any other journal.
Journal of Pharmacy & Pharmacognosy Research (JPPRes) es una revista internacional, especializada y con revisión por pares, la cual publica estudios científicos en los campos farmacéuticos y farmacognósticos, relacionados con la física, botánica, química, propiedades biológicas, toxicológicas y aplicaciones clínicas de entidades moleculares, ingredientes farmacéuticos activos, dispositivos y sistemas de administración de medicamentos, vacunas y productos biológicos, incluyendo su diseño, fabricación, evaluación y comercialización. Esta revista publica artículos de investigación, revisiones, comentarios y cartas al editor, así como ediciones especiales y las reseñas de libros y tesis de pre y postgrado de los farmacéuticos o los profesionales que intervienen en Ciencias Farmacéuticas y Farmacognosia. JPPRes tiene una tasa de aceptación de 40%. El tiempo promedio entre la presentación del manuscrito y la decisión final es de 60 días y el tiempo medio entre la aceptación final y la publicación es de 15 días. Los manuscritos presentados a JPPRes se aceptan solamente en el entendimiento de que son objeto de revisión editorial y que no han sido, ni serán, publicados en su totalidad o en parte en cualquier otra revista.
Editorial Office (for submission queries and papers under review, according with the JPPRes Instructions):
[email protected] Executive Editor (for accepted and published papers only):
[email protected] EDITOR-IN-CHIEF Gabino Garrido, Departamento de Ciencias Farmacéuticas, Universidad Católica del Norte, Antofagasta, Chile. E-mail:
[email protected] EXECUTIVE EDITOR Marisela Valdés González, Departamento de Ciencias Farmacéuticas, Universidad Católica del Norte, Antofagasta, Chile. E-mail:
[email protected] EDITORIAL AND DESIGN MANAGER Xavier Garrido Valdés. Universidad Santo Tomás, Antofagasta, Chile.
© 2017 Journal of Pharmacy & Pharmacognosy Research ISSN 0719-4250 http://jppres.com/jppres
EDITORIAL BOARD MEMBERS Antonio Vasallo (Università degli Studi della Basilicata, Potenza, Italia) Bárbara B. Garrido Suárez (Centro de Investigaciones y Desarrollo de Medicamentos, La Habana, Cuba) Carla Delporte (Universidad de Chile, Santiago, Chile) Damaris Silveira (Universidade de Brasília, Brasília, Brazil) Douglas S. de A. Chaves (Universidade Federal Rural do Rio de Janeiro, Rodovia, Brazil) Edgar Pastene (Universidad de Concepción, Concepción, Chile) Etile Spegazzini (Universidad de Belgrano, Buenos Aires, Argentina) Farid Chemat (Avignon University, Avignon, France) Fidel O. Castro (Universidad de Concepción, Chillán, Chile) Guilherme N. L. do Nascimento (Universidade Federal de Tocantins, Tocantins, Brazil) Jacqueline Sepúlveda (Universidad de Concepción, Concepción, Chile) Jelena Nadinic (Universidad de Buenos Aires, Buenos Aires, Argentina) José H. Isaza-Martínez (Universidad del Valle, Cali, Colombia) Juan C. Sepúlveda-Arias (Universidad Tecnológica de Pereira, Pereira, Colombia) Madan M. Gupta (The University of the West Indies, Trinidad & Tobago, West Indies) Mahomoodally M. Fawzi (University of Mauritius, Réduit, Mauritius) María Inés Isla (Universidad Nacional de Tucuman, Tucumán, Argentina) Marisol Fernández Alfonso (Universidad Complutense de Madrid, Madrid, España) Mayank Gangwar (Banaras Hindu University, Varanasi, India) Silvia Debenedetti (Universidad de Belgrano, Buenos Aires, Argentina) Vikash Kumar Ruhil (PDM College of Pharmacy, Haryana, India) Yasser Shahzad (University of Huddersfield, Huddersfield, United Kingdom) REVIEWERS The reviewers will be recruited among researchers and clinicians with high international reputation in Pharmacy and Pharmacognosy and supported by members of the editorial board. Abstracted/indexed in: Web of Science™ Core Collection Emerging Sources Citation Index (ESCI), Scopus, EMBASE, LATINDEX, Directory of Open Access Journal, Qualis CAPES, REDALYC, REDIB, Google Scholar, PERIODICA, BIBLAT, HINARI, Index Copernicus, Chemical Abstract, SHERPA/RoMEO, ResearchGate.
© 2017 Journal of Pharmacy & Pharmacognosy Research, 5 (5), 270-277, 2017 ISSN 0719-4250 http://jppres.com/jppres Original Article | Artículo Original
Quercus infectoria and Terminalia chebula decrease melanin content and tyrosinase activity in B16/F10 cell lines [Quercus infectoria y Terminalia chebula disminuyen el contenido de melanina y la actividad tirosinasa en las líneas celulares B16/F10] Akram Jamshidzadeh1, Yaser Shokri2**, Nahid Ahmadi1, Neda Mohamadi3, Fariba Sharififar3* 2Herbal
1Toxicology Department, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran. and Traditional Medicines Research Center, Department of Pharmacognosy, Kerman University of Medical Sciences, Kerman, Iran. 3Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. E-mail: *
[email protected], **
[email protected]
Abstract
Resumen
Context: One of the most complained skin cares in ethnic skin like Asian women is hyperpigmentation, and lightening preparations have been long-standing desired. Due to the side effects of current drugs, medicinal plants have attracted more attentions as a source of novel drugs. Mazo (Quercus infectoria galls) and Terminalia chebula fruits have been suggested in Persian Traditional Medicine as a safe treatment for hyperpigmentation.
Contexto: Uno de los cuidados de la piel más reclamados en la piel étnica de las mujeres asiáticas es la hiperpigmentación, y se han deseado, desde hace mucho tiempo, preparaciones de aclaramiento. Debido a los efectos secundarios de los fármacos actuales, las plantas medicinales han atraído más atenciones como fuente de nuevos fármacos. Mazo (agallas de Quercus infectoria) y frutos de Terminalia chebula han sido sugeridos en la medicina tradicional persa como un tratamiento seguro para la hiperpigmentación.
Aims: To evaluate the cytotoxicity and the effect on melanin synthesis in B16/F10 melanoma of Q. infectoria and T. chebula extracts. Methods: After collection and scientific authentication, plants were extracted by maceration method with methanol and were standardized based on total phenolic content. MTT assay and colorimetric method were used for cytotoxicity and determination of melanin content and tyrosinase activity in B16/F10 cells, respectively. Kojic acid was used as a reference compound. Results: Total phenolic content of Q. infectoria and T. chebula was determined as 287.34 ± 4.21 and 172.61 ± 8.67 mg gallic acid equivalent/g dried extract, respectively. Both plants decreased cell viability at 100 µg/mL and significantly reduced intercellular melanin to 66.25% and 71.1%, respectively in comparison to kojic acid (56.63%) at 50 µg/mL. In the same concentration, 65.7% and 71.2% tyrosinase activity was inhibited by Q. infectoria and T. chebula, which significantly were different from control (p 1 indicates that cytotoxicity on tumor cell lines surpassed that on healthy non-tumor cells (Nugroho et al., 2013). Statistical analysis Mean inhibitory concentration (IC50) was calculated through 95% of confidential interval by a linear regression equation and statistical analysis was performed using Graph-Pad Prism 5.0 software. The global comparison was performed using two-way variance analysis (ANOVA) followed by BonferJ Pharm Pharmacogn Res (2017) 5(5): 322
Rojas et al.
roni's test for multiple comparisons. P
