Ã. Extrusion Modulates. Spontaneous Hippocampal Network Events in Perinatal Rats and Mice. Inkeri Spoljaric, Albert Spoljaric, Martina Mavrovic, Patricia Seja, ...
Cell Reports, Volume 26
Supplemental Information
KCC2-Mediated Cl Extrusion Modulates Spontaneous Hippocampal Network Events in Perinatal Rats and Mice Inkeri Spoljaric, Albert Puskarjov, and Kai Kaila
Spoljaric,
Martina
Mavrovic,
Patricia
Seja,
Martin
Figure S1. KCC2 modulates spontaneous network events in P5 rat hippocampus (related to Figure 1) Effect of VU0463271 (10 µM) on GDPs in the P5 rat hippocampal CA3 area. Sample LFP trace (filtered 1-10 Hz); and quantification of normalized network activity after application of VU0463271. Data are presented as median and IQR, Wilcoxon signed-rank test was used for statistical analysis, *p < 0.05, n value indicated in the bar diagram.
Figure S2. Bumetanide blocks GDPs in the presence of VU0463271 (related to Figure 1) Effect of bumetanide (10 µM) on GDPs in the perinatal rat (P0-5) hippocampal CA3 area in the presence of VU0463271 (10 µM). Bumetanide blocked GDPs in 11/11 recordings. Sample LFP trace from a P0 rat (filtered 1-10 Hz).
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Figure S3. Effect of VU0463271 on GDP frequency and amplitude in perinatal mice and rats (related to Figure 1) Quantification of the effect of VU0463271 (10 µM) on normalized frequency and amplitude of GDPs. In P0-2 rats and KCC2+/+ mice, VU0463271 caused a marked increase in both GDP frequency and amplitude (P0-2 rats: frequency fold change 2.30 [IQR 1.39-3.23], p = 0.001; amplitude fold change 1.16 [IQR 1.06-1.30], p = 0.005. KCC2+/+ mice: frequency fold change 1.86 [IQR 1.38-2.43] p = 0.003; amplitude fold change 1.33 [IQR 0.99-1.80] p = 0.033). In P5 rats only the change in frequency was statistically significant (frequency fold change 1.91 [IQR 1.79-2.56] p = 0.018; amplitude fold change 1.15 [IQR 1.02-1.70] p = 0.063). In KCC2-/- mice, neither frequency nor amplitude of GDPs were affected by VU0463271 (frequency fold change 1.00 [IQR 0.66-1.16] p = 0.176; amplitude fold change 1.06 [IQR 0.88-1.18] p = 0.508). Data are presented as median and IQR, Wilcoxon signed-rank test was used for statistical analysis, *p < 0.05,**p < 0.01, N.S. = non-significant, n values are indicated in the bar diagrams.
