Leptin enhances feeding suppression and neural activation produced ...

Am J Physiol Regul Integr Comp Physiol 289: R473–R477, 2005. First published April 28, 2005; doi:10.1152/ajpregu.00835.2004.

Leptin enhances feeding suppression and neural activation produced by systemically administered bombesin Ellen E. Ladenheim, M. Emond, and T. H. Moran Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Baltimore, Maryland Submitted 13 December 2004; accepted in final form 14 April 2005

Ladenheim, Ellen E., M. Emond, and T. H. Moran. Leptin enhances feeding suppression and neural activation produced by systemically administered bombesin. Am J Physiol Regul Integr Comp Physiol 289: R473–R477, 2005. First published April 28, 2005; doi:10.1152/ajpregu.00835.2004.—Leptin amplifies feeding inhibition and neural activation produced by either cholecystokinin or intragastric preloads, suggesting that leptin may increase the efficacy of gastrointestinal meal-related signals. To determine whether leptin would similarly potentiate the feeding inhibitory actions of another putative satiety peptide, we evaluated the effects of third ventricular leptin administration on food intake and c-Fos activation in response to systemically administered bombesin (BN). Leptin (3.5 ␮g) was administered 1 h before either 0.9% saline or BN (0.32 and 1.0 nmol/kg) followed by 30-min access to Ensure liquid diet. Although neither leptin nor 0.32 nmol/kg BN alone suppressed Ensure intake, the combination reduced intake by 28%. The higher BN dose (1.0 nmol/kg) produced a significant suppression by itself but was further enhanced in the presence of leptin. Consistent with the behavioral results, c-Fos activation in the nucleus of the solitary tract was increased by combined dosages of leptin and 0.32 nmol/kg BN beyond the individual response to either peptide. In the presence of leptin, BN produced a 3.4- to 5.2-fold increase in the number of c-Fos-positive cells in the nucleus of the solitary tract compared with when BN was given alone. These data provide further support for the hypothesis that the effect of leptin on food intake may be mediated, in part, by modulating meal-related satiety signals. brain-gut peptide; satiety; hindbrain; nucleus of the solitary tract

in response to nutrient ingestion have been implicated in the control of food intake by eliciting signals that limit the size of individual meals and result in meal termination (11, 36). Other signals, such as leptin, are secreted in direct proportion to adiposity and convey long-term information related to body energy stores (22). Although these controls were long thought to be distinct, there is increasing evidence to support the idea that the overall regulation of food intake and body weight results from integration of meal-related satiety signals and long-term energy stores. Several laboratories have demonstrated that exogenous administration of leptin selectively decreases meal size without affecting meal frequency or intermeal interval (7, 9, 13). This pattern is similar to that produced by meal-related satiety signals, suggesting that leptin may exert its effects on food intake through a mechanism that alters the efficacy of withinmeal satiety signals (8, 12, 27). A number of studies have demonstrated a synergistic interaction between leptin and the meal-related satiety peptide

GUT PEPTIDES THAT ARE SECRETED

Address for reprint requests and other correspondence: E. E. Ladenheim, Johns Hopkins Univ. School of Medicine, Dept. of Psychiatry and Behavioral Sciences, Ross 618, 720 Rutland Ave., Baltimore, MD 21205 (e-mail: [email protected]). http://www.ajpregu.org

cholecystokinin (CCK) in food intake and body weight (1, 8, 23, 24, 27). We (8) previously reported that feeding suppression by peripheral CCK administration was significantly increased by central leptin administration and that the combined treatment resulted in activation of brain stem neurons that were not responsive to either peptide individually. A complementary study by McMinn et al. (27) demonstrated that leptin deficiency generated by fasting decreased the ability of CCK to reduce food intake but that its satiety effect was restored by leptin replacement. Together, these results have lent further support to the overall hypothesis that leptin may participate in controlling food intake by either enhancing, or dampening, the responsiveness of neurons to meal-generated satiety signals. Bombesin (BN) is an amphibian-derived peptide that is closely related to the mammalian peptide gastrin-releasing peptide (GRP) (25, 33). Like CCK, BN has been proposed as a meal-contingent satiety signal (10). BN-like immunoreactivity is found in abundance in the gastrointestinal tract and central nervous system of mammals (5, 29, 33, 34) and is increased in the stomach and several brain regions in response to food ingestion (14, 28, 32). Systemic administration of BN-related peptides inhibits food intake in a dose-related fashion, under a variety of experimental conditions and in a number of mammalian species, including humans (11, 21, 30). Exogenous administration of BN, like CCK, decreases food intake by reducing meal size and, at low doses, does not prolong the intermeal interval (35). Furthermore, mice with a targeted deletion of the receptor for the mammalian BN-like peptide, GRP, exhibit an increase in meal size (16). Because BN’s effects on feeding share many characteristics with those of CCK, we hypothesized that leptin may also influence food intake through an interaction with BN-like peptides. Therefore, in the present study, we addressed whether centrally administered leptin would alter the ability of peripherally administered BN to decrease food intake in a short-term feeding paradigm. In addition, we evaluated the effect of these treatments on neuronal activation in the nucleus of the solitary tract (NTS) and area postrema (AP), a brain region that is critically important to BN’s feeding effects and exhibits increased c-Fos expression following intraperitoneal BN administration (4, 17, 18, 20). METHODS

Subjects. All experiments used male Sprague-Dawley rats (Charles River, Kingston, NY) weighing 250 –300 g at the start of experimentation. They were individually housed in hanging wire mesh cages in a temperature-controlled room (22–23°C) on a 12:12-h light-dark

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cycle. Unless otherwise specified, pelleted rat chow and tap water were available ad libitum. All behavioral testing was performed in the rat’s home cage in the light portion of the light-dark cycle. All procedures were approved by the Institutional Animal Care and Use Committee at Johns Hopkins University. Surgery. Rats were anesthetized with a mixture of ketamine (150 mg/kg)-xylazine (30 mg/kg) given intramuscularly and mounted into a stereotaxic apparatus with the skull level. Stainless steel 23-gauge guide cannulas aimed at the third cerebral ventricle were implanted at a 10° angle at a position 1 mm lateral to midline 1 mm posterior to bregma, and 5.5 mm ventral to dura. The cannulas were anchored to the skull using three stainless steel screws and acrylic dental cement. Cannulas were blocked with a 30-gauge stainless steel obturator when not in use. Behavioral verification of cannula placement. Approximately 1 wk after surgery, cannula placement was verified by measuring water consumption in response to third ventricular infusion of ANG II (10 ng in 3 ␮l; Sigma, St. Louis, MO). Only animals that drank ⱖ5 ml in the 30 min after ANG II administration were considered to have viable cannula placements and used in the subsequent experiment (n ⫽ 32). Feeding paradigm. Rats were then adapted to a feeding schedule in which they had rat chow removed each day at 9:00 AM and returned at 4:00 PM. At 3:00 PM, they were presented with a nutritionally complete liquid diet (Ensure, Ross Products, Columbus, OH), and intake was measured for 30 min. Feeding experiments commenced when Ensure intake had stabilized. To examine the effects of leptin on BN-induced suppression of food intake, rats received a third ventricular injection of either human recombinant leptin (3.5 ␮g in 3.5 ␮l; PeproTech, Rocky Hill, NJ) or its vehicle (0.9% saline) via a Gilmont microliter syringe attached to PE-10 tubing and a 30-gauge stainless steel injector that extended 1.5 mm below the cannula tip. One hour later, rats were injected intraperitoneally with either 0.9% saline or BN (0.32 nmol/kg and 1.0 nmol/kg; Bachem, King of Prussia, PA) in a volume of 1 ml/kg and were immediately given access to Ensure liquid diet for 30 min. Thus the experimental conditions for each animal were as follows: icv saline ⫹ ip saline, icv saline ⫹ ip BN (0.32 and 3.2 nmol/kg), icv leptin ⫹ ip saline, icv leptin ⫹ ip BN (0.32 and 3.2 nmol/kg). Data analyses. Only animals that had a positive response to the ANG II test and correct cannula placement, as verified postmortem by histological examination, were included in the data analyses. Data were subjected to repeated-measures ANOVA (3 ⫻ 2) analyses. Differences between means from each experimental condition were determined by planned t-test comparisons. c-Fos immunohistochemistry. At the conclusion of the behavioral experiments, a subgroup of rats (n ⫽ 17) was then evaluated for the activation of c-Fos protein in the caudal hindbrain (AP and NTS) in response to the leptin and BN treatments. In accordance with treatment group, rats were injected with either 0.9% saline or leptin (3.5 ␮g) into the third ventricle 1 h before intraperitoneal administration of either 0.9% saline or BN (0.32 nmol/kg). The four groups were as follows: icv saline-ip saline (n ⫽ 3), icv leptin-ip saline (n ⫽ 4), icv saline-ip BN (n ⫽ 4), and icv leptin-ip BN (n ⫽ 6). Because feeding and gastric distension induce c-Fos activation in the brain regions of interest, all injections were done in the absence of food, and food was not returned after the second injection. Seventy-five minutes after the intraperitoneal injection, rats were anesthetized with Euthasol (pentobarbital sodium and phenytoin; Delmarva Laboratories, Midlothian, VA) and perfused transcardially with 200 ml of 0.1 M PBS (pH 7.4) followed by 200 ml of 4% paraformaldehyde in PBS. The fixed brains were removed and placed in 4% paraformaldehyde containing 25% sucrose for 24 – 48 h. Serial 40-␮m frozen sections were cut coronally through the beginning at the most caudal aspect of the AP and ending in the rostral NTS. The sections were then incubated in 0.3% hydrogen peroxide for 60 min, thoroughly washed in PBS, and preabsorbed with normal goat AJP-Regul Integr Comp Physiol • VOL

serum in 0.1 M PBS containing 0.1% Triton X-100. Sections were incubated for 48 h at 4°C in rabbit anti-c-Fos antiserum (1:30,000; Oncogene Science, San Diego, CA) and sequentially incubated in biotinylated goat anti-rabbit IgG and avidin-biotin-peroxidase complex (Elite Vectastain kit, Vector Labs, Burlingame, CA). We visualized the reaction product using nickel sulfate-enhanced diaminobenzidine as the chromagen. Sections were mounted onto gelatin-coated slides, dehydrated in alcohols, and coverslipped. Analyses of c-Fos in the AP-NTS. Images of tissue sections were digitized, and areas of interest were outlined based on cellular morphology. c-Fos-positive nuclei within the regions of interest were quantified with automated image analysis software (IpLab, Scanalytics, Fairfax, VA). The four caudal hindbrain regions evaluated were at the mid-AP level and three levels of the NTS (one matched section from each level), corresponding to the coordinates in the brain atlas of Paxinos and Watson (31) of approximately ⫺14.08, ⫺13.68, and ⫺13.3 mm caudal to bregma. The effect of leptin and BN on c-Fos activation at each brain site was then compared across treatment groups by 2 ⫻ 2 ANOVA (peptide treatment by leptin treatment) followed by post hoc Tukey’s tests to evaluate differences between individual means. RESULTS

Effect of leptin on BN-induced suppression of food intake. The interaction of leptin with BN to reduce 30-min Ensure intake is demonstrated in Fig. 1. The ANOVA indicated a significant dose effect of BN [F(2,62) ⫽ 19.967, P ⬍ 0.0001], as well as a significant interaction between BN and leptin [F(2,62) ⫽ 8.496, P ⬍ 0.0005]. When leptin or the low BN dose (0.32 nmol/kg) was given alone, there was no significant suppression of Ensure intake (P ⬎ 0.05). However, when leptin was administered into the third ventricle 1 h before intraperitoneal 0.32 nmol/kg BN administration, Ensure intake was significantly reduced by 28% (P ⬍ 0.01). The higher BN dose (1.0 nmol/kg) produced a significant suppression of Ensure intake (15.7%) compared with intake

Fig. 1. Effect of combined dosages of leptin and bombesin (BN) on 30-min Ensure intake. Data are expressed as means ⫹ SE under the following conditions: icv saline-ip saline (SAL/SAL), icv sal-0.32 nmol/kg ip BN (SAL/BN.32), 3.5 ␮g icv leptin-ip saline (LEP/SAL), 3.5 ␮g icv leptin-0.32 nmol/kg ip BN (LEP/BN.32), icv sal-1.0 nmol/kg ip BN (SAL/BN1.0), and 3.5 ␮g icv leptin-1.0 nmol/kg ip BN (LEP/BN1.0). *Significantly different from SAL/SAL (P ⬍ 0.01); #significantly different from SAL/BN.32 (P ⬍ 0.01); ⫹significantly different from SAL/BN1.0 (P ⬍ 0.05). 289 • AUGUST 2005 •

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after saline injection (P ⬍ 0.01). The combination of leptin and BN elicited a significantly greater reduction of Ensure intake (29.7%, P ⬍ 0.05) than when either peptide was given alone. This suppression did not represent a further reduction in intake from the combination of leptin and the low dose of BN (P ⬎ 0.05). Effect of leptin on BN-induced c-Fos activation in the AP-NTS. The effect of combined treatments of intracerebroventricular leptin and intraperitoneal BN on c-Fos activation in the caudal hindbrain is shown in Fig. 2. The results were consistent with those of the food intake experiment. Post hoc analyses revealed that neither BN nor leptin significantly increased c-Fos expression compared with the comparable saline-treated condition (P ⬎ 0.05). However, when 3.5 ␮g of leptin were administered into the lateral ventricle 1 h before intraperitoneal injection of 0.32 nmol/kg BN, significant peptide-leptin interactions were seen at all levels of the NTS [NTS1, F(1,16) ⫽ 5.32, P ⬍ 0.05; NTS2, F(1,16) ⫽ 4.77, P ⬍ 0.05; NTS3, F(1,16) ⫽ 6.46, P ⬍ 0.05]. The number of c-Fos-positive cells in the caudal (NTS1), mid (NTS2), and rostral (NTS3) areas was significantly increased by 4.8-, 5.2-, and 3.4-fold, respectively, compared with when BN was given in the absence of leptin (P ⬍ 0.05). The distribution of c-Fos-positive cells in the NTS was similar to that previously described in that most of the c-Foslike immunoreactivity was confined to the medial subnucleus with minimal labeling in the commissural subnucleus (4, 20). There were no differences detected in the number of c-Fospositive cells in the AP after any treatment (P ⬎ 0.05). DISCUSSION

The results of this experiment demonstrate that, when leptin is administered centrally 1 h before peripheral administration of BN, the reduction of food intake is significantly greater than when either peptide is given alone. These results support the hypothesis that leptin, a long-term signal involved in energy homeostasis, may affect food intake and body weight by modifying meal-generated satiety signals such as BN.

Fig. 2. Number of c-Fos positive cells in the area postrema (AP) and nucleus of the solitary tract (NTS) after administration of icv saline-ip saline (SAL/ SAL), 3.5 ␮g icv leptin-ip saline (LEP/SAL), icv saline-0.32 nmol/kg ip BN (SAL/BN), and 3.5 ␮g icv leptin-0.32 nmol/kg ip BN (LEP/BN). Results are means ⫹ SE. Measurements were taken corresponding approximately to the following coordinates relative to bregma: AP ⫽ ⫺13.68 mm, NTS1 ⫽ ⫺14.08 mm, NTS2 ⫽ ⫺13.68 mm, and NTS3 ⫽ ⫺13.3 mm. *Significantly different from LEP/SAL (P ⬍ 0.05); ⫹significantly different from SAL/BN (P ⬍ 0.05). AJP-Regul Integr Comp Physiol • VOL

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A number of studies have supported the notion that either metabolic status or obesity can alter the ability of a variety of peptides to affect food intake, including BN. For example, studies examining the effects of food deprivation on BN satiety demonstrated that BN was less effective in suppressing food intake in food-deprived than in normally fed rats (2, 15). Moreover, rats chronically fed a high-fat diet are less responsive to the feeding inhibitory effects of BN than those fed a low-fat diet (6). Finally, genetically obese (ob/ob) mice have deficits in feeding suppression produced by exogenous BN administration (26). All of these studies represent a decrease in leptin signaling due to either diminished leptin levels (as in fasting) or reduced sensitivity to leptin (as with high-fat diets or obesity), suggesting that impaired sensitivity to BN may be the result of reduced BN and leptin interactions. We chose to use BN in this study instead of the mammalian BN-like peptide GRP because it has a longer duration of action, similar to that of CCK. As mentioned previously, BN is an amphibian peptide that is structurally similar to the mammalian peptide GRP. Unlike GRP, BN binds with equal and high affinity to two mammalian BN receptor subtypes: BB2, which has a high affinity for GRP, and BB1, which has a high affinity for neuromedin B (38). Several lines of evidence suggest that BN-induced suppression of feeding is mediated by both receptor subtypes (19, 37). Therefore, by using BN in the present study, we cannot discern whether the feeding effects that we observed with our combined treatment was due to leptin enhancing suppression induced by BN’s interaction with one or both receptor subtypes. A previous study examining the effects of combined doses of leptin and BN in mice reported a failure to detect an interaction to reduce food intake (1). There are several differences between that study and the present experiment that may explain this result. In the prior study, the earliest time point in which feeding was measured was 1 h after administration. Because the majority of feeding suppression by BN occurs in the first 30 min after administration and declines shortly thereafter, the 1-h time point may not have been optimal to observe an interaction. Moreover, the timing of the leptin injection and the route of administration may be critical factors in revealing a synergistic interaction. In their study, both the leptin and BN were injected intraperitoneally and were administered simultaneously, whereas in the present experiment the leptin was given centrally 1 h before intraperitoneal administration of BN. Although the mechanism(s) responsible for the interaction of leptin and BN on feeding suppression is unknown, it is possible that brain sites sensitive to leptin may require activation before gastrointestinal peptide stimulation to initiate a chain of neuronal events that would enhance sensitivity to neurons responsible for BN’s feeding effects. Thus our experimental design of providing leptin 1 h before BN administration would facilitate such an interaction. The importance of timing is consistent with other studies revealing positive interactions between leptin and CCK (3, 8). Results from the combined treatment of leptin and BN on c-Fos activation in the caudal hindbrain paralleled those of the behavioral experiments. When leptin or BN was administered alone, there was no significant induction of c-Fos. However, when leptin and BN were given in combination, 289 • AUGUST 2005 •

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there was a substantial increase in c-Fos immunoreactivity in the medial NTS. Unlike our previous results with CCK (8), there was no significant increase in c-Fos immunoreactivity in the AP after the combined treatment. The distribution of c-Fos in the NTS after the leptin-BN combination is consistent with previous reports examining c-Fos activation in the caudal hindbrain after higher doses of intraperitoneal BN that potently reduced food intake (4, 20). The similarity in distribution of combined leptin-BN treatment with that produced by a higher dose of BN alone suggests that leptin may increase the responsiveness of the same neuronal population. Although these data support a role for the NTS in the interaction between leptin and BN, they do not address which leptin receptor population is responsible for mediating this effect. We have previously suggested that leptin and CCK’s synergistic effect on food intake may be mediated through a leptin-sensitive descending hypothalamic pathway that projects to caudal hindbrain neurons to enhance the response to meal-related gastrointestinal stimuli (8). Because of the reliance of intraperitoneal BN’s feeding effects on NTS neurons (17, 18), this mechanism would also be concordant with the results of the present experiment. However, we cannot rule out the possibility of a local site of action of leptin because leptin administered into the third ventricle would also gain access to leptin receptors in the NTS. In summary, we found that prior administration of intracerebroventricular leptin significantly enhanced the feeding inhibitory effects of systemically administered BN and produced parallel results on neuronal activation within the NTS. These results are consistent with those that we have previously reported examining leptin and CCK interactions and further support the hypothesis that one mechanism through which leptin may reduce food intake is by amplifying sensitivity to gastrointestinal satiety signaling. ACKNOWLEDGMENTS The authors thank Ross Products, a division of Abbott Laboratories, for the generous donation of Ensure liquid diet. Present address of Michael Emond: Laurentian University, Department of Psychology, Sudbury, Ontario, Canada P3E 2C6.

GRANTS This work was supported by National Institute of Diabetes and Digestive and Kidney Diseases Grants DK-46448 and DK-19302.

REFERENCES 1. Barrachina MD, Martinez V, Wang L, Wei JY, and Tache Y. Synergistic interaction between leptin and cholecystokinin to reduce short-term food intake in lean mice. Proc Natl Acad Sci USA 94: 10455–10460, 1997. 2. Billington CJ, Levine AS, and Morley JE. Are peptides truly satiety agents? A method of testing for neurohumoral satiety effects. Am J Physiol Regul Integr Comp Physiol 245: R920 –R926, 1983. 3. Blevins JE, Schwartz MW, and Baskin DG. Evidence that paraventricular nucleus oxytocin neurons link hypothalamic leptin action to caudal brain stem nuclei controlling meal size. Am J Physiol Regul Integr Comp Physiol 287: R87–R96, 2004. 4. Bonaz B, De Giorgio R, and Tache Y. Peripheral bombesin induces c-fos protein in the rat brain. Brain Res 600: 353–357, 1993. AJP-Regul Integr Comp Physiol • VOL

5. Brown M, Allen R, Villarreal J, Rivier J, and Vale W. Bombesin-like activity: radioimmunologic assessment in biological tissues. Life Sci 23: 2721–2728, 1978. 6. Covasa M and Ritter RC. Rats maintained on high-fat diets exhibit reduced satiety in response to CCK and bombesin. Peptides 19: 1407– 1415, 1998. 7. Eckel LA, Langhans W, Kahler A, Campfield LA, Smith FJ, and Geary N. Chronic administration of OB protein decreases food intake by selectively reducing meal size in female rats. Am J Physiol Regul Integr Comp Physiol 275: R186 –R193, 1998. 8. Emond M, Schwartz GJ, Ladenheim EE, and Moran TH. Central leptin modulates behavioral and neural responsivity to CCK. Am J Physiol Regul Integr Comp Physiol 276: R1545–R1549, 1999. 9. Flynn MC, Scott TR, Pritchard TC, and Plata-Salaman CR. Mode of action of OB protein (leptin) on feeding. Am J Physiol Regul Integr Comp Physiol 275: R174 –R179, 1998. 10. Gibbs J, Fauser DJ, Rowe EA, Rolls BJ, Rolls ET, and Maddison SP. Bombesin suppresses feeding in rats. Nature 282: 208 –210, 1979. 11. Gibbs J and Smith GP. The actions of bombesin-like peptides on food intake. Ann NY Acad Sci 547: 210 –216, 1988. 12. Havel PJ. Peripheral signals conveying metabolic information to the brain: short-term and long-term regulation of food intake and energy homeostasis. Exp Biol Med (Maywood) 226: 963–977, 2001. 13. Kahler A, Geary N, Eckel LA, Campfield LA, Smith FJ, and Langhans W. Chronic administration of OB protein decreases food intake by selectively reducing meal size in male rats. Am J Physiol Regul Integr Comp Physiol 275: R180 –R185, 1998. 14. Kateb CC and Merali Z. A single meal elicits regional changes in bombesin-like peptide levels in the gut and brain. Brain Res 596: 10 –16, 1992. 15. Kraly FS, Miller LA, and Gibbs J. Diurnal variation for inhibition of eating by bombesin in the rat. Physiol Behav 31: 395–399, 1983. 16. Ladenheim EE, Hampton LL, Whitney AC, White WO, Battey JF, and Moran TH. Disruptions in feeding and body weight control in gastrin-releasing peptide receptor deficient mice. J Endocrinol 174: 273– 281, 2002. 17. Ladenheim EE and Ritter RC. Caudal hindbrain participation in the suppression of feeding by central and peripheral bombesin. Am J Physiol Regul Integr Comp Physiol 264: R1229 –R1234, 1993. 18. Ladenheim EE and Ritter RC. Low-dose fourth ventricular bombesin selectively suppresses food intake. Am J Physiol Regul Integr Comp Physiol 255: R988 –R993, 1988. 19. Ladenheim EE, Wirth KE, and Moran TH. Receptor subtype mediation of feeding suppression by bombesin-like peptides. Pharmacol Biochem Behav 54: 705–711, 1996. 20. Li BH and Rowland NE. Peripherally and centrally administered bombesin induce Fos-like immunoreactivity in different brain regions in rats. Regul Pept 62: 167–172, 1996. 21. Lieverse RJ, Jansen JB, van de Zwan A, Samson L, Masclee AA, Rovati LC, and Lamers CB. Bombesin reduces food intake in lean man by a cholecystokinin-independent mechanism. J Clin Endocrinol Metab 76: 1495–1498, 1993. 22. Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S, Kern PA, and Friedman JM. Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects. Nat Med 1: 1155–1161, 1995. 23. Matson CA, Reid DF, Cannon TA, and Ritter RC. Cholecystokinin and leptin act synergistically to reduce body weight. Am J Physiol Regul Integr Comp Physiol 278: R882–R890, 2000. 24. Matson CA, Wiater MF, Kuijper JL, and Weigle DS. Synergy between leptin and cholecystokinin (CCK) to control daily caloric intake. Peptides 18: 1275–1278, 1997. 25. McDonald TJ, Jornvall H, Nilsson G, Vagne M, Ghatei M, Bloom SR, and Mutt V. Characterization of a gastrin releasing peptide from porcine nonantral gastric tissue. Biochem Biophys Res Commun 90: 227–233, 1979. 26. McLaughlin CL and Baile CA. Obese mice and the satiety effects of cholecystokinin, bombesin and pancreatic polypeptide. Physiol Behav 26: 433– 437, 1981. 27. McMinn JE, Sindelar DK, Havel PJ, and Schwartz MW. Leptin deficiency induced by fasting impairs the satiety response to cholecystokinin. Endocrinology 141: 4442– 4448, 2000. 289 • AUGUST 2005 •

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LEPTIN ENHANCES BOMBESIN SATIETY 28. Merali Z and Kateb CC. Rapid alterations of hypothalamic and hippocampal bombesin-like peptide levels with feeding status. Am J Physiol Regul Integr Comp Physiol 265: R420 –R425, 1993. 29. Moody TW, O’Donohue TL, and Jacobowitz DM. Biochemical localization and characterization of bombesin-like peptides in discrete regions of rat brain. Peptides 2: 75–79, 1981. 30. Muurahainen NE, Kissileff HR, and Pi-Sunyer FX. Intravenous infusion of bombesin reduces food intake in humans. Am J Physiol Regul Integr Comp Physiol 264: R350 –R354, 1993. 31. Paxinos G and Watson C. The Rat Brain in Stereotaxic Coordinates (2nd ed.). Orlando, FL: Academic, 1986. 32. Plamondon H and Merali Z. Push-pull perfusion reveals meal-dependent changes in the release of bombesin-like peptides in the rat paraventricular nucleus. Brain Res 668: 54 – 61, 1994. 33. Polak JM, Bloom SR, Hobbs S, Solcia E, and Pearse AG. Distribution of a bombesin-like peptide in human gastrointestinal tract. Lancet 1: 1109 –1110, 1976.

AJP-Regul Integr Comp Physiol • VOL

R477

34. Roth KA, Weber E, and Barchas JD. Distribution of gastrin releasing peptide— bombesin-like immunostaining in rat brain. Brain Res 251: 277–282, 1982. 35. Rushing PA, Gibbs J, and Geary N. Vena caval bombesin infusion decreases spontaneous meal size in undisturbed rats. Physiol Behav 59: 307–310, 1996. 36. Smith GP and Gibbs J. Satiating effect of cholecystokinin. Ann NY Acad Sci 713: 236 –241, 1994. 37. Stratford TR, Gibbs J, and Smith GP. Simultaneous administration of neuromedin B-10 and gastrin-releasing peptide(1–27) reproduces the satiating and microstructural effects of bombesin. Peptides 17: 107–110, 1996. 38. Von Schrenck T, Heinz-Erian P, Moran T, Mantey SA, Gardner JD, and Jensen RT. Neuromedin B receptor in esophagus: evidence for subtypes of bombesin receptors. Am J Physiol Gastrointest Liver Physiol 256: G747–G758, 1989.

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