RESEARCH ARTICLE
Low-Dose Bisphenol-A Impairs Adipogenesis and Generates Dysfunctional 3T3-L1 Adipocytes Fabiana Ariemma1, Vittoria D’Esposito1,2, Domenico Liguoro1,2, Francesco Oriente1, Serena Cabaro1, Antonietta Liotti1, Ilaria Cimmino1, Michele Longo1, Francesco Beguinot1,2, Pietro Formisano1,2, Rossella Valentino1,2* 1 Department of Translational Medical Sciences, Federico II University of Naples, via S. Pansini, 5, 80131, Naples, Italy, 2 URT Genomic of Diabetes, Institute of Experimental Endocrinology and Oncology (IEOS), National Council of Research (CNR), via S. Pansini, 5, 80131, Naples, Italy *
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Abstract OPEN ACCESS Citation: Ariemma F, D’Esposito V, Liguoro D, Oriente F, Cabaro S, Liotti A, et al. (2016) Low-Dose Bisphenol-A Impairs Adipogenesis and Generates Dysfunctional 3T3-L1 Adipocytes. PLoS ONE 11(3): e0150762. doi:10.1371/journal.pone.0150762 Editor: Angel Nadal, Universidad Miguel Hernández de Elche, SPAIN Received: December 10, 2015 Accepted: February 18, 2016 Published: March 4, 2016 Copyright: © 2016 Ariemma et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper. Funding: This work was supported in part by MIURPRIN (Ministero dell'Istruzione, dell'Università e della Ricerca-Progetti di Ricerca di Interesse Nazionale) [prot. 2010MCLBCZ], MIUR-FIRB MERIT (Ministero dell'Istruzione, dell'Università e della Ricerca-Fondo per gli Investimenti della Ricerca di Base Medical Research in Italy)[RBNE08NKH7], Associazione Italiana per la Ricerca sul Cancro [AIRC n: IG 12136], European Foundation for the Study of Diabetes [EFSD, EFSD Diabetes and Cancer Program 2011]. The funders had not role in study design, data
Environmental endocrine disruptors (EDCs), including bisphenol-A (BPA), have been recently involved in obesity and diabetes by dysregulating adipose tissue function. Our aim was to examine whether prolonged exposure to low doses of BPA could affect adipogenesis and adipocyte metabolic functions. Therefore, 3T3-L1 pre-adipocytes were cultured for three weeks with BPA 1nM to mimic human environmental exposure. We evaluated BPA effect on cell proliferation, differentiation, gene expression and adipocyte metabolic function. BPA significantly increased pre-adipocyte proliferation (p
