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Mar 24, 2015 - Methods. We analyzed LPCAT1 mRNA and protein expression levels in OSCC-derived cell lines. Immunohistochemistry was performed to ...
RESEARCH ARTICLE

Lysophosphatidylcholine Acyltransferase1 Overexpression Promotes Oral Squamous Cell Carcinoma Progression via Enhanced Biosynthesis of Platelet-Activating Factor Tomomi Shida-Sakazume1, Yosuke Endo-Sakamoto2, Motoharu Unozawa1, Chonji Fukumoto1, Ken Shimada2, Atsushi Kasamatsu2, Katsunori Ogawara2, Hidetaka Yokoe3, Masashi Shiiba4, Hideki Tanzawa1,2, Katsuhiro Uzawa1,2*

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1 Department of Oral Science, Graduate School of Medicine, Chiba University, Chiba, Japan, 2 Department of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, Chiba, Japan, 3 Department of Oral and Maxillofacial Surgery Research Institute, National Defense Medical College, Saitama, Japan, 4 Department of Medical Oncology, Graduate School of Medicine, Chiba University, Chiba, Japan * [email protected]

Abstract OPEN ACCESS Citation: Shida-Sakazume T, Endo-Sakamoto Y, Unozawa M, Fukumoto C, Shimada K, Kasamatsu A, et al. (2015) Lysophosphatidylcholine Acyltransferase1 Overexpression Promotes Oral Squamous Cell Carcinoma Progression via Enhanced Biosynthesis of Platelet-Activating Factor. PLoS ONE 10(3): e0120143. doi:10.1371/journal. pone.0120143 Academic Editor: Chih-Hsin Tang, China Medical University, TAIWAN Received: October 10, 2014 Accepted: January 19, 2015 Published: March 24, 2015 Copyright: © 2015 Shida-Sakazume et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: These authors have no support or funding to report. Competing Interests: The authors have declared that no competing interests exist.

Background The relevance of lysophosphatidylcholine acyltransferase1 (LPCAT1), a cytosolic enzyme in the remodeling pathway of phosphatidylcholine metabolism, in oral squamous cell carcinoma (OSCC) is unknown. We investigated LPCAT1 expression and its functional mechanism in OSCCs.

Methods We analyzed LPCAT1 mRNA and protein expression levels in OSCC-derived cell lines. Immunohistochemistry was performed to identify correlations between LPCAT1 expression levels and primary OSCCs clinicopathological status. We established LPCAT1 knockdown models of the OSCC-derived cell lines (SAS, Ca9-22) for functional analysis and examined the association between LPCAT1 expression and the platelet-activating factor (PAF) concentration and PAF-receptor (PAFR) expression.

Results LPCAT1 mRNA and protein were up-regulated significantly (p