Dec 14, 2009 - 2Laboratory of Hematopiesis, EA3855, Faculty of Medicine, University Francois Rabelais,. 10 Boulevard Tonnellé, 37032 Tours, France.
Macrophage and osteoblast responses to biphasic calcium phosphate microparticles Borhane Hakim Fellah,1 Bruno Delorme,2 Je´roˆme Sohier,1 David Magne,3 Pierre Hardouin,3 Pierre Layrolle1 1 Inserm, U791, Laboratory for Osteoarticular and Dental Tissue Engineering, Faculty of Dental Surgery, University of Nantes, 1 Place Alexis Ricordeau, 44042 Nantes, France 2 Laboratory of Hematopiesis, EA3855, Faculty of Medicine, University Francois Rabelais, 10 Boulevard Tonnelle´, 37032 Tours, France 3 Laboratory of Cellular and Molecular Biology, EA2603, IFR114, Boulevard Napole´on, 62327 Boulogne sur Mer, France Received 16 October 2008; revised 17 September 2009; accepted 22 September 2009 Published online 14 December 2009 in Wiley InterScience (www.interscience.wiley.com). DOI: 10.1002/jbm.a.32663 Abstract: The aim of this work was to investigate in vitro the biological events leading to ectopic bone formation in contact with microporous biphasic calcium phosphate (BCP) ceramics. After implantation, microparticles may arise from their degradation and induce an inflammatory response involving macrophages. The secretion of pro-inflammatory cytokines may affect the differentiation of osteoblasts. Mouse macrophage-like (J774) and osteoblast-like (MC3T3-E1) cells were cultured in the presence of BCP microparticles of different sizes (
