Articles in PresS. Am J Physiol Regul Integr Comp Physiol (October 28, 2004). doi:10.1152/ajpregu.00206.2004
1
Maternal protein restriction leads to hyperinsulinemia and reduced insulin signalling protein expression in 21 month-old female rat offspring.
DS Fernandez-Twinn, A Wayman, S Ekizoglou, MS Martin, CN Hales, SE Ozanne
Department of Clinical Biochemistry, Level 4, Addenbrookes Hospital, Hills Road, Cambridge CB2 2QR, U.K.
Running head : Insulin resistance in female growth-restricted rats
Corresponding author: Dr. D S Fernandez-Twinn Department of Clinical Biochemistry, Box 232, Level 4, Addenbrookes Hospital, Hills Road, Cambridge CB2 2QR UK Fax: 44-1223-330598 Email:
[email protected]
Copyright © 2004 by the American Physiological Society.
2 Abstract Human adult diseases such as cardiovascular disease, hypertension and type-2 diabetes have been epidemiologically linked to poor fetal growth and development. Male offspring of rat dams fed a low protein (LP) diet during pregnancy and lactation develop diabetes with concomitant alterations in their insulin signalling mechanisms. Such associations have not been studied in female offspring. The aim of this study was to determine if female LP offspring develop diabetes in later life. Control and LP female offspring groups were obtained from rat dams fed a control (20% protein) or an isocaloric (8% protein) diet respectively, throughout pregnancy and lactation. Both groups were weaned and maintained on 20% normal laboratory chow until 21 months of age when they underwent intravenous glucose tolerance testing (IVGTT). Fasting glucose was comparable between the 2 groups, however, LP fasting insulin was approximately 2-fold that of controls (p
