Media composition of photosynthetic bacteria namely

Media composition of photosynthetic bacteria namely, Rhodobacter viridis and Rhodobacter capsulatus. COMPONENTS/L 1.

KH2PO4 – 0.5 gm

2.

MgSO4.7H2O – 0.2 gm

3.

NaCl – 0.4 gm

4.

NH4Cl

5.

CaCl2.2H2O

6.

SL7 (Trace elements solution)- 1 ml SL7 (Composition of trace elements solution/L) i) ii) iii) iv) v) vi) vii) viii)

7.

1ml of 25% HCl (v/v) ZNCl2 - 7 mg MnCl2.4H2O - 100 mg H3BO3 60 mg CoCl2.6H2O 200 mg CuCl2.H2O 20 mg NiCl2. 6H2O 20 mg NaMoO4.6H2O - 40 mg Malate/Pyruvate - 22 mM

8.

Yeast extract -

0.2 gm

9.

Ferric Citrate -

0.1% (w/v)

10.

Vitamin B 12

- 1 ml of 20 mg/100 ml

pH of the prepared media was adjusted to 6.8