Plant tissue culture is one of most important part of biotechnology. Terminalia arjuna is a widely used medicinal plant throughout India and popular in various ...
Micro propagation of Terminalia arjuna Harishankar mahto1 & Kavita singh2 Department of Biotechnology, Central university of Rajasthan1,Rajasthan, India Bio-sky research laboratory2, Ranchi, Jharkhand, India
ABSTRACT Plant tissue culture is one of most important part of biotechnology. Terminalia arjuna is a widely used medicinal plant throughout India and popular in various indigenous system in Ayurveda, Sidha, Unani. Indian system of medicine bark is used as astringent cooling, cardiotonic, tonic in fracture ulcer , spermatorrhoea, diabetes, cough, tumour, excessive perspiration asthama inflamation and skin disorder. In order to achieve callus induction from leaf of Terminalia arjuna different hormonal combination were tried. Mature leaf did not respond well; however, juvenile leaf gave good response. Out of various media combination tried, the explant showed callus induction on supplemented medium(MS media) contains1mg/litre NAA along with 1mg/litre BAP . Callusing was observed after about 4-5 weeks of culture. However, NAA along with BAP was found most suitable for induction & proliferation of callus. It was found that even several repeating subculture of immature leaf explant did not reduce an ability of inducing callus. These finding means that it is expected that large number of plantlet can be constructed. This indicates that calluses are considered more efficient & useful as a material for construction of plantlets.
