Modified Provasoli
Stocks
per litre (1) PII trace metals Na2 EDTA H3BO3 MnSO4.H2O ZnSO4.7H2O CoSO4.7H2O (2) IronEDTA Fe(NH4)2(SO4)2.6H2O Na2 EDTA
Medium Na2 ßglycero PO4.5H2O NaNO3 IronEDTA (2) Vitamin B12 Thiamine Biotin PII trace metals (1)
1.0 g 1.12 g 0.12 g 0.022 g 0.005 g 0.7 g 0.6 g
Stock
per litre medium
50 g/litre 35 g/litre
8.0 ml 110 ml 100 ml 8.75 ml 8.0 ml 8.0 ml 200 ml
0.01 g/litre 0.5 g/litre 0.005 g/litre
To prepare final medium, dispense the above into 10 ml aliquots and sterilize by autoclaving. Finally, to use add 10 ml per litre to sterile 30 ppt filtered seawater.
Reference West JA & McBride DL (1999) Long term and diurnal carpospore discharge patterns in the Ceramiaceae, Rhodomelaceae and Delesseriaceae (Rhodophyta). Hydrobiologia. 398399, 101114.
media recipes
April 2004
CCAP (Culture Collection of Algae and Protozoa), Dunstaffnage Marine Laboratory, Oban, Argyll, PA37 1QA, UK Tel: +44 (0)1631 559000 Fax: +44 (0)1631 559001 Email:
[email protected] Web: www.ccap.ac.uk
