Molecular mechanisms underlying the function of ...

Molecular mechanisms underlying the function of Magnaporthe oryzae AvrPib as revealed by population genetic, comparative genomic and mutagenic approaches Shulin Zhang, Ling Wang, Weihuai Wu, Liyun He, Xianfeng Yang, and Qinghua Pan

Table S1 PCR primer sequences used in the study Primer name

Forward primer

MS6-24

GCGAGTTTACAGGTTTAGCA

Enzyme site

Reverse primer TTTGGGGCTAATAACAGACC

MS6-3

CCACATCGCAGCCTCACTTG

CCAGTCGGTTCCAGGTCTTG

MS6-17

CAAGAATGCAGAGAACCACAG

GTTTTCCGCTGTGTAAATAGA

MS6-7

CCAAGGCACCAGCACAGACC

CAGAAGATGCCTCGGAGATC

MS6-10

CAACATGTGGTGTCTGATCC

GCAGGGCCCACTACACATGT

CAG6-23

TGCGTTGTGGCGGTAGTCA

GTTTGTCTGGTCAGCGTCAC

MS3-5

GAGTTTGAGACCTTGCGATT

GGCGGCAAGTCGCTGAAGG

MS6-34

CGGATGATGGATGAATTGGC

AGTGCCGATGCTCCAGAAT

CAG6-21

ACCTCGCATTGTCGGCATTC

TACACCGAGGAACTTGCTTGG

CAG6-26

GGACAAGGGAGGCAAATCTAAC

ATGCCGACAATGCGAGGTAT

CAG6-25

ATGCGATTTCTGCGTTGCTA

CCGAGATACAAAACGCTACCCT

MS3-26

GAAGGCGACAAGTGGCAGT

CTCGTCTGGTATCAGTTGCC

MS3-6.16

CGAATATGCAAATGTAAGTT

GAGTACTTGCCAGAGAGAA

MS3-6.15

ATTTCGGTTGATCGTGTTGG

GCCACCTGTTTCTCCAA

MS3-6.13

CCCAAGGTAGGTACGAGAAA

TTTCTACTGCCAGACAAG

Enzyme site

Use of primers Gene mapping

Gene cloning b1

TTGGCGCGCCCGTTTGGTTACGTTCACCCTTG

AscI

TAGGCGCGCCTCGACTACATCCCCATGACGC

AscI

b2

TTGGCGCGCCGCCATCAACGAGACCACAGCCCAC

AscI

TTGGCGCGCCACTGCGGGAATGCCGACAATGCGAG

AscI

b3

ATGGCGCGCCGGAGAATGAGCTGCGAGGTC

AscI

TAGGCGCGCCACTGCGGGAATGCCGACAATGCGAG

AscI Genotyping

AvrPibF1/R1


ATGCCGACAATGCGAGGTAT

AvrPibF2/R2

TGGAGAAGACTTTGATGC

GAACGCATAATGGCAACTA Mutations

Mab-1

TTTTGCGATGCAAGCTCTCGGTAT

AAGGCGATAGTGATAAAAGTGG

Mab-2

TCTTGCGATGCAAGCTCTCGGTAT


Mab-3

TAGTGCGATGCAAGCTCTCGGTAT


Mab-4

TTTATCACTATCGCCTTTACTGC

AGTGGTTGAGGAACGCATAAT

Mab-5

CTTTTGTGCGATGCAAGCTCT

GCGATAGTGATAAAAGTGGTT

Mab-6

TTTTATCACTATCGCCTTTTCTGC

GTGGTTGAGGAACGCATAAT

Mab-7

CTTTCGTGCGATGCAAGCTCT


Mab-8

CTTTCCTGCGATGCAAGCTCT


Mab-9

TTTTATCACTATCGCCTTTTGC

GTGGTTGAGGAACGCATAAT

Mab-10

ATTGCGATGCAAGCTCTCGGTATT

AATGGCAACTAATTTAATTGTATA

Mab-11

ACACAGGTTACTATCTTGAA

AATAAAGGCGATAGTGATAAAAGTGG

Mab-12

ACACAGGTTACTATCTTGAAGAAGGGT

AATGGCAACTAATTTAATTGTATA

Mab15

TATTTTACTATTAGTGTGTCGGAC

TTTTCCACGGATGTTAAATTCTCT

Mab16

GAATCCAGAAAATTTAACATCCGTG

CCCCTTTGGAACTTCTACC Gene knock out (Mab13)

AvrPib-up

GCAGCGGCCGCAGACTCAAAGTTCCATAGC

NotI

CATAAGCTT AAACAAGGGATGCTGACTAC

Hind

AvrPib-down

GCAGGATCCGTTAGTACCTACAGCTCACAAG

BamHI

GCAGCGGCCGCGCTGGATGGTTATTGCGAT

NotI

AvrPib-Hpt

GCAGCGGCCGCTATTGAAGGAGCATTTTTGG

NotI

GTAGCGGCCGCGCTCTTGTTCGGTCGGCATC

NotI

AvrPib


ATACCTCGCATTGTCGGCAT Pot3 domain-swapping

Mab14

TT AAGCTT AGACTGGCGTCGTGACTGGA

Hind III

GAACGCATAATGGCAACTA

BstB I qPCR analysis

Pot2

ACGACCCGTCTTTACTTATTTGG

AAGTACGCTTGGTTTTGTTGGAT

ubiquitin

AACCAGCTGAGGCCCAAGA

ACGATTGATTTAACCAGTCCATGA RT-PCR analysis

Actin

GCGGTTACACCTCTCTACCAC

AGTCTGGATCTCCTGCTCAAAG

RT-AvrPib

GGGTAGAAGTTCCAAAGGGG

TCCACGGTATATTTGCTGCCA

Note: Sequences underlined indicate restrict sites for plasmid constructions, and those boldfaced indicate mutation sites.

Table S2 Details of the 300 Mo isolates collected from five provinces of China, and the 12 chosen isolates, which are pathogenic on grass species other than rice Growing Sampled

Genotype c

Sampled

Sampled Pib-infection

Isolate area a cultivar Guangdong (GD) population (V/3: 13) CHL2345 GD I Mabayinzhan CHL2357 GD I Shanyou 428 CHL2370 GD II Jinshanyou 3182 CHL2375 GD I Shanyou 253 CHL2384 GD I Changguidao

county

timeb

Qujiang Wengyuan Longchuan Renhua Lianping

2008E 2008E 2008E 2008E 2008E

A V V V V

3 1 1 1 0

3 1 0 1 1

3 1 1 1 1

CHL2386 CHL2387 CHL2394 CHL2401

GD II GD II GD II GD II

Fengsimiao Shanyou 428 Nuo Huayougui 99

Gaoyao Meixian Meixian Wuhua

2008E 2008E 2008E 2008E

V V V V

3 0 0 1

3 1 0 0

3 1 0 1

D37-38, A137G (E46G)

CHL2409

GD II

Zhongerruanzhan

Longmen

2008E

V

3

3

3

D37-38

CHL2412 CHL2416 CHL2417

GD II GD II GD II

Shanyougui 99 Meixiangzhan Tianyou 290

Fengkai Xinxing Xingning

2008E 2008E 2008E

V V MV

3 2 0

3 2 3

3 2 3

D37-38

CHL2419

GD II

Changguidao

Jiexi

2008E

V

3

3

3

D37-38

CHL2421 CHL2426 CHL2433 CHL2436 CHL2438 CHL2441 CHL2442 CHL2444 CHL2446

GD III GD III GD III GD III GD III GD III GD III GD III GD III

Sanyinian Youyou 389 Huayou 86 Huayou 86 Changguidao Zajiaodao Tianyou 998 Guangyinzhan Shuanghuangzhan

Yangjiang Xinyi Xinyi Xinyi Gaozhou Gaozhou Haifeng Foshan Jiedong

2008E 2008E 2008E 2008E 2008E 2008E 2008E 2008E 2008E

V V V V V V A V V

3 0 1 0 0 1 3 1 1

3 1 1 1 0 1 3 0 1

3 1 1 1 0 1 3 1 1

D37-38

type

Mutation

AvrPib

AvrPibF1/R1 AvrPibF2/R2 Integrated in CDS d

T38G (I13S)

resequencing e 26

G166A (V56I)

CHL2448

GD III

Teyou 721

Chaoan

2008E

V

1

1

1

CHL2449 CHL2452

GD II GD III

Zengyouzhan Fengqin

Zengcheng Lianjiang

2008E 2008E

V V

3 1

3 1

3 1

D37-38

CHL2455 CHL2457

GD III GD III

Bairizao Zhanyou 226

Huidong Huidong

2008E 2008E

V V

3 1

3 1

3 1

D37-38

CHL2458 CHL2464 CHL2467 CHL2486 CHL2490 CHL2492 CHL2497

GD III GD III GD I GD I GD I GD I GD I

Teyou 721 Taishanbai Meixiangzhan Fengyou 998 Guodao No.1 Yueyou 360 Meixiangzhan

Yangchun Kaiping Renhua Lechang Ruyuan Nanxiong Qujiang

2008E 2008E 2008L 2008L 2008L 2008L 2008L

V A V V V V A

3 3 2 1 1 0 3

3 3 2 1 1 0 3

3 3 2 1 1 0 3

D37-38

CHL2508 CHL2509 CHL2513 CHL2516 CHL2518 CHL2534 CHL2547 CHL2549

GD I GD I GD I GD I GD II GD II GD II GD II

Youyou 308 Shanyou 89 Shanyou 46 Shanyou 82 Yuxiang 88 Shanyou 122 Tianyou 368 Shanyou 122

Wengyuan Pingyuan Xinfeng Jiaoling Longchuan Meixian Fengshun Dapu

2008L 2008L 2008L 2008L 2008L 2008L 2008L 2008L

V V V V V V V V

3 1 0 1 0 1 1 0

3 1 1 1 1 1 1 1

3 1 1 1 1 1 1 1

D37-38

CHL2551 CHL2560 CHL2561 CHL2563

GD II GD III GD II GD III

Boyouyun 3 Huanghuazhan Longyou 665 Zhanzinuo

Zijin Boluo Fougang Huidong

2008L 2008L 2008L 2008L

V A V V

3 3 0 2

3 3 1 2

3 3 1 2

D37-38

CHL2570 CHL2581 CHL2584 CHL2586

GD II GD II GD II GD II

Yesizhan Qiuyou 452 Zayounuo Zhenguizhong

Longmen Qingxin Huaiji Yingde

2008L 2008L 2008L 2008L

V V V A

3 1 1 3

3 0 1 3

3 1 1 3

D37-38

CHL2593 CHL2600 CHL2604 CHL2606 CHL2608 CHL2615 CHL2626

GD II GD II GD III GD III GD III GD III GD III

Qiuyou 3008 Qiuyou 3008 Guinongzhan Xiaonongzhan Taiwanyouzhan Bo II You 15 Longyou 673

Yunfu Deqing Haifeng Zhuhai Yangchun Lianjiang Xinyi

2008L 2008L 2008L 2008L 2008L 2008L 2008L

V V V A V V V

1 1 1 3 1 1 1

0 0 1 3 3 1 1

1 1 1 3 1 1 1

CHL2631 GD III Shanyou 3550 CHL2636 GD IV Bo II You 15 Hunan (HN) population (V/3: 8) EHL0312 HN I Tefu No.7 EHL0313 HN I Tefu No.7

Gaozhou Suixi

2008L 2008L

V V

3 1

3 1

3 1

Taoyuan Taoyuan

2007 2007

V A

1 3

1 3

1 3

EHL0314

HN II

Weiyou 402

Anren

2007

V

3

3

3

D37-38

EHL0317 EHL0319

HN II HN II

Weiyou 402 Liangyou 287

Anren Liling

2007 2007

V V

4 1

4 3

4 1

D39-66, A120G (P40P)

EHL0321

HN II

V402

Hengnan

2007

V

3

3

3

D37-38

EHL0323

HN II

V233

Hengdong

2007

V

3

3

3

D37-38

EHL0324

HN II

I You 402

Hengdong

2007

V

3

3

3

D37-38

EHL0326 EHL0327 EHL0329 EHL0330 EHL0331 EHL0337 EHL0338 EHL0339 EHL0340

HN II HN II HN II HN II HN II HN II HN II HN II HN II

I You 402 Zhuliangyou 30 Douyuanyou 299 T You 99 Xianfeng 501 Jinyou 71 Jinyou 898 Nongping 001 Weiyou 507

Hengnan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan

2007 2007 2007 2007 2007 2007 2007 2007 2007

V V V V V V V V V

3 1 1 1 1 2 0 2 0

3 1 1 1 1 2 0 2 0

3 1 1 1 1 2 0 2 0

D37-38, C207T (S69S)

D37-38 T201C (S67S) 30

G136A (E46K)

EHL0341 EHL0342 EHL0345 EHL0346 EHL0348 EHL0349 EHL0350 EHL0353 EHL0354 EHL0355 EHL0356 EHL0360 EHL0361 EHL0363 EHL0364 EHL0365 EHL0367 EHL0368 EHL0369 EHL0370 EHL0372 EHL0373 EHL0374 EHL0377 EHL0378 EHL0379 EHL0380 EHL0381

HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN II HN III HN III HN III HN III HN III HN I HN I HN I HN I HN I

Weiyou 298 Weiyou 463 T You 213 T You 103 Jinyou 103 Jinyou 402 Xianfeng No.1 Nongfengyou 909 Jinyou 821 Weiyou 463 Zhongyou No.1 Zhongyou No.1 Zhuliangyou 02 Xiangnuo T You 706 Zhongyou 493 Jinyou 463 Zhuliangyou 83 Jinyou 899 Jinyou 463 Nuogu Jinyou 463 Shennongdao 101 Jing No.5 Wanxiang No. 13 Pin No. 3 Wannuo Wannuo

Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Dongan Daoxian Daoxian Daoxian Daoxian Daoxian Yiyang Taojiang Taojiang Yiyang Yiyang

2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007

V V V V V V V V V V V V V A V A V V V V V V A V A V V A

3 4 1 1 2 1 0 1 0 1 1 1 1 3 0 3 1 0 1 0 0 1 3 1 3 0 1 3

3 4 1 3 2 1 1 1 1 1 1 3 3 3 1 3 1 1 1 0 0 1 3 3 3 1 1 3

3 4 1 1 2 1 1 1 1 1 1 1 1 3 1 3 1 1 1 0 0 1 3 1 3 1 1 3

D37-38 D39-66, G90C (K30N),A120G(P40P),G123A(K41K)

EHL0382 EHL0385 EHL0386 EHL0387 EHL0393

HN I HN II HN II HN II HN II

Nuogu II You 58 Minghui 63 II You 838 Y NO. 232

YueYang Liuyang Liuyang Liuyang Youxian

2007 2007 2007 2007 2007

V V V V V

0 0 1 1 1

0 1 1 1 1

0 1 1 1 1

EHL0394 EHL0395 EHL0963 EHL0964

HN II HN IV HN I HN I

Xinshanyou 64 Zhongyou 288 Xianghuayou No. 7 Xianghuayou No. 8

Anren Huaihua Taojiang Taojiang

2007 2007 2008 2008

V A V V

4 3 1 1

4 3 3 3

4 3 1 1

D39-66, A120G (P40P)

EHL0975

HN I

Fuyou II

Taojiang

2008

V

3

3

3

D37-38

Changsha Changsha Zhuzhou Zhuzhou Liling

2008 2008 2008 2008 2008

V V V V V

3 0 1 1 0

3 0 1 1 1

3 0 1 1 1

D37-38

EHL0979 HN II Mixiang No.1 EHL0982 HN II Xiangwanxian No.17 EHL0988 HN II Chaojidao 527 EHL0989 HN II Xiangwanxian No.13 EHL0990 HN II Xiangzaoxian No.24 Liaoning (LN) population (V/3: 0) EHL0396 LN I Minxi 12 EHL0397 LN I Chenhong 59 EHL0400 LN I Lige EHL0409 LN I Yanjing 167 EHL0412 LN I Huanghai No.6 EHL0431 LN I Liaodan 203 EHL0433 LN I Liaohan 109 EHL0444 LN II Chenhong 59 EHL0445 LN II Minxi 12 EHL0446 LN II Gangyu 129 EHL0447 LN II Koshihikari EHL0468 LN II Maoyang No.3

18 Zhuanghe Zhuanghe Zhuanghe Donggang Donggang Donggang Donggang Pulandian Pulandian Pulandian Pulandian Fengchen

2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006

A V A V V V V A V V A V

3 1 3 1 1 1 1 3 1 0 3 1

3 3 3 1 1 1 1 3 1 1 3 1

3 1 3 1 1 1 1 3 1 1 3 1


LN II LN II LN II LN II LN II LN II LN II LN II LN II LN II LN II LN II LN III LN III LN III LN III LN III LN III LN III LN I LN I LN I LN I LN I LN II LN II LN II LN II

Qiao 201-2 Panjin 78-2 Chenhe No.1 Yuanfeng No.9 Fuyou 504 Shennong 0705 Jijing 88 Xin No.2 Shennong 315 Su 01-18 Yanfeng Yanfeng 47-12 Chenhe No.1 Maoyang No.3 Liaohe 12 Yanjing 228 Liaohe No.1 Huadan 995 Huadan 995 Liaoxing No.1 Gangyu No.2 Chenhe No.1 Maoyang No.5 Liaojing No.9 Heixiangdao Zhuangyan No.8 Koshihikari Yanfeng 47

Fengchen Fengchen Fengchen Fushun Fushun Fushun Fushun Fushun Fushun Dashiqiao Dashiqiao Dashiqiao Panjin Panjin Panjin Panjin Panjin Shenyang Tieling Dandong Dandong Dandong Dandong Dandong Dalian Dalian Dalian Dalian

2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2007 2007 2007 2007 2007 2007 2007 2007 2007

V V V V V A A V A V V V V V V V V V V V V V V V V V V A

1 1 1 1 1 3 3 1 3 1 1 1 1 1 1 0 1 1 1 0 1 0 1 1 1 1 1 3

1 3 1 1 1 3 3 1 3 1 1 1 1 3 1 1 1 1 1 0 1 0 1 1 1 1 1 3

1 1 1 1 1 3 3 1 3 1 1 1 1 1 1 1 1 1 1 0 1 0 1 1 1 1 1 3

EHL0921 LN II Gangyuan No.8 EHL0922 LN II Liaonong 06-7 EHL0924 LN II Fu 9906 EHL0925 LN II Fuxing 90 EHL0926 LN II Jijing 88 EHL0927 LN II Daohuaxiang EHL0928 LN II Liaonong 06-7 EHL0931 LN II Jiudao 46 EHL0932 LN II Xinyu No.3 EHL0935 LN II Jijing 88 EHL0936 LN II Shendao 37 EHL0937 LN II Shennong 315 EHL0939 LN II Xinyi No.3 EHL0940 LN II Fuxing 90 EHL0941 LN II Xinyi No.3 EHL0942 LN II Shennong 9903 EHL0943 LN II Huadan No.995 EHL0944 LN II Fuxing 90 EHL0961 LN IV Chenhe No.1 EHL0962 LN IV Yanjing 218 Jilin (JL) population (V/3: 0) CHL678 JL I Tong 31 CHL680 JL I Ji 04 CHL682 JL I Tong 313 CHL684 JL I Fengyou 201 CHL686 JL I Pingjing 1 CHL688 JL I Jiu 01 C 6 CHL690 JL I Changbai 9

Dalian Qinyuan Qinyuan Qinyuan Qinyuan Qinyuan Xinbin Xinbin Xinbin Xinbin Xifeng Xifeng Xifeng Xifeng Kaiyuan Kaiyuan Kaiyuan Kaiyuan Suizhong Suizhong

2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007

V V V V V A A A A A V V A V V V V V V V

1 1 0 1 0 3 3 3 3 3 1 1 3 1 1 0 1 1 1 1

1 1 0 1 1 3 3 3 3 3 1 1 0 1 1 1 1 1 1 1

1 1 0 1 1 3 3 3 3 3 1 1 3 1 1 1 1 1 1 1

T173C (V58A) 16

Yongji Yongji Yongji Yongji Yongji Yongji Shulan

2003 2003 2003 2003 2003 2003 2003

A A A A A A A

3 3 3 3 3 3 3

3 3 3 3 3 3 3

3 3 3 3 3 3 3

CHL692 CHL693 CHL695 CHL697 CHL699 CHL700 CHL701 CHL702 CHL703 CHL704 CHL705 CHL708 CHL710 CHL712 CHL714 CHL715 CHL716 CHL718 CHL721 CHL722 CHL724 CHL725 CHL730 CHL731 CHL733 CHL734 CHL735 CHL736

JL I JL I JL I JL I JL I JL I JL I JL I JL I JL I JL I JL IV JL IV JL IV JL IV JL IV JL IV JL IV JL I JL I JL I JL I JL I JL I JL I JL I JL I JL I

Dongnong V 7 Yan 312 Songjing 6 301 Tongyu 12 Wuyou 1 99-35 Tongfeng No. 3 Ji 01-3628 Pingjing No. 2 Chang 6 Tongjing 612 Yan 313 Changbai 9 Wuyou C Yannian No. 1 Jiudao 16 Changlixiang 12 Jiu 01 D 13 Ji T620-4 T 02 Tongjing 790 B Tongnian 3 Tongyu 308 Tongnian 23 Tong 3158 Daliwang Unknown

Shulan Shulan Shulan Shulan Shulan Shulan Panshi Panshi Panshi Panshi Panshi Jiaohe Jiaohe Jiaohe Jiaohe Jiaohe Jiaohe Jiaohe Jilin Jilin Jilin Jilin Jilin Jilin Meihekou Liuhe Liuhe Lishu

2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003

A A A A A A A A A A A A A A A V V A A A V V A A V A A A

3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 0 1 3 3 3 1 1 3 3 1 3 3 3

3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 1 1 3 3 3 1 1 3 3 1 3 3 3

3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 1 1 3 3 3 1 1 3 3 1 3 3 3

CHL737 JL I Unknown CHL739 JL I 182 CHL740 JL I 183 CHL741 JL I No. 55 CHL742 JL I No. 55 CHL743 JL I No. 52 CHL744 JL I Tong 793 CHL745 JL I Tong 190 CHL746 JL I Menggudao CHL748 JL I Changxiangsi CHL749 JL I Tongjing 769 CHL751 JL I Tongyin 58 CHL752 JL I Zupei 11 CHL754 JL I Chaochan 2 CHL755 JL I Unknown CHL756 JL I Xueguang CHL757 JL IV Unknown CHL759 JL III Shangyu No. 397 CHL760 JL I Menggudao CHL761 JL I 403 CHL762 JL I 112 CHL764 JL I Tong 458 CHL765 JL I Unknown CHL766 JL I Unknown CHL767 JL I 939 Heilongjiang (HLJ) population (V/3: 0) EHL0524 HLJ III Songjing 7 EHL0527 HLJ II Kendao 10

Yitong Jiutai Jiutai Jiutai Jiutai Jiutai Dehui Dehui Dongfeng Dongfeng Dongfeng Dongfeng Dongfeng Gongzhuling Yitong Yongji Jiaohe Helong Meihekou Liuhe Liuhe Dongfeng Gongzhuling Gongzhuling Baichen

2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2003 2002 2002 2002 2002 2002 2002 2002 2002 2002 2002

A V V V V V V V A A V A A V A A A A A A A A A A A

3 0 0 1 1 1 1 1 3 3 1 3 3 1 3 3 3 3 3 3 3 3 3 3 3

3 1 1 1 1 1 1 1 3 3 1 3 3 1 3 3 3 3 3 3 3 3 3 3 3

3 1 1 1 1 1 1 1 3 3 1 3 3 1 3 3 3 3 3 3 3 3 3 3 3 18

Zhaoyuan Anqin

2006 2006

V V

1 1

1 1

1 1


HLJ II HLJ I HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ III HLJ III HLJ III HLJ III HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ II HLJ I HLJ II

Song 98-131 Songjing 10 Kendao 12 Kuyuku 131 Suijing No.4 Dongnong 220 Puyou 18 Wuyoudao No.3 Wuyoudao No.3 Mudanjiang 19 Kendao 10 Shangyu 397 Punian No.7 Kenjiandao No.6 Kuyuku 131 Kendao 12 Kuyuku 131 Suijing No.4 Kuyuku 131 Longdun 02-318 Kuyuku 131 Kuyuku 131 Longdun 102 Kuyuku 131 Kuyuku 131 Kuyuku 131 Kuyuku 131 Kuyuku 131

Anqin Achen Tonghe Tonghe Tonghe Shangzhi Shangzhi Shangzhi Shangzhi Shangzhi Shangzhi Muling Hulin Hulin Hulin Hulin Mishan Mishan Mishan Jidong Tangyuan Huachuan Jiamusi Jiamusi Baoqin Jixian Mudanjiang Tangyuan

2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006 2006

V V V A A A V A A A A A A A V A A A A A A V A A A A A A

1 1 1 3 3 3 1 3 3 3 3 3 3 3 1 3 3 3 3 3 3 1 3 3 3 3 3 3

1 1 1 3 3 3 1 3 3 3 3 3 3 3 1 3 3 3 3 3 3 1 3 3 3 3 3 3

1 1 1 3 3 3 1 3 3 3 3 3 3 3 1 3 3 3 3 3 3 1 3 3 3 3 3 3

T160C (F54L)


HLJ II HLJ II HLJ II HLJ II HLJ I HLJ I HLJ I HLJ III HLJ III HLJ I HLJ III HLJ III HLJ III HLJ I HLJ I HLJ II HLJ I HLJ I HLJ I HLJ I HLJ II HLJ II HLJ II HLJ II HLJ III HLJ III HLJ III HLJ III

Kendao No.6 Kendao No.11 Kuyuku 131 Longjing 17 Kendao No.6 Kuyuku 131 Longjing 17 Kenjiandao 13 Kuyuku 131 Jite 639 Songjing 3 Songjing 12 Jijing 106 Songjing 5 Songjing 6 Kendao 12 Menggudao Menggudao Shangyu 397 Shangyu 397 Kuyuku 131 Kuyuku 131 Puyou 9 Puyou 9 Longjing 17 Longjing 17 Kenjiandao 10 Kenjiandao 10

Tangyuan Qinan Qinan Qinan Mudanjiang Mudanjiang Mudanjiang Jiansanjiang Jiansanjiang Wuchang Zhaodong Zhaodong Zhaodong Shuangchen Shuangchen Fangzheng Mudanjiang Mudanjiang Mudanjiang Mudanjiang Muling Muling Muling Muling Hulin Hulin Hulin Hulin

2006 2006 2006 2006 2006 2006 2006 2006 2006 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007 2007

A A A A A A A A A A V V V V V V A A A A A A A A A A A A

3 3 3 3 3 3 3 3 3 3 0 1 1 1 1 1 3 3 3 3 3 3 3 3 3 3 3 3

3 3 3 3 3 3 3 3 3 3 1 1 1 1 1 1 3 3 3 3 3 3 3 3 3 3 3 3

3 3 3 3 3 3 3 3 3 3 1 1 1 1 1 1 3 3 3 3 3 3 3 3 3 3 3 3

T116C (V39A)

EHL0870 HLJ III Longjing 17 Hulin 2007 A 3 3 3 EHL0871 HLJ III Longjing 17 Hulin 2007 A 3 3 3 Barley and weed derived isolates 5 Smoeng, Thailand 1998 CHL1 N/A Barley 3 3 3 Smoeng, Thailand 1998 CHL2 N/A Barley 3 3 3 Smoeng, Thailand 1998 CHL27 N/A Weed 3 3 3 Smoeng, Thailand 1998 CHL30 N/A Weed 3 3 3 Smoeng, Thailand 1998 CHL31 N/A Weed 3 3 3 Smoeng, Thailand 1998 CHL32 N/A Weed 3 3 3 Guangzhou, China 2001 CHL325 N/A Weed 3 3 3 Guangzhou, China CHL326 N/A Weed 2001 3 3 3 Guangzhou, China CHL329 N/A Weed 2001 3 3 3 Guangzhou, China 2001 CHL330 N/A Weed 3 3 3 Guangzhou, China 2001 CHL331 N/A Weed 0 3 3 Guangzhou, China 2001 CHL332 N/A Weed 0 3 3 a The rice-growing areas in the respective provinces, China, were adopted from Regionalization of Rice Cropping in China Edited in 1988 by China National Rice Research Institute, Hangzhou, China. Abbreviations GD I and GD II mean #1 and #2 rice-growing areas, respectively, in Guangdong province and so on. b

2008E and 2008L mean Early season (from March to July) and Late season (from July to November) in 2008, respectively and so on.

c

PCR product-based genotypes 0, 1, 2, 3, and 4, respectively, indicate none, highest, second higher, normal, and double binds of AvrPib products (also see Fig. 2).

d e

D37-38, deletion at A37T38; A137G, SNP at #137 position and so on. Isolates being phenotypes/genotypes of both the major ones (V/1 and A/3) were subjected to selective resequencing, those being other three ones (V/2, V/3, and V/4) were all subjected to resequencing. A, avirulent; V, virulent, MV, moderately virulent.