OneSTEP Ladder 500. (0.5-5 kbp). OneSTEP Ladder 1 kb. (1-10 kbp). OneSTEP
Ladder. Supercoiled Plasmid. Gene Ladder 100. (0.1-2 kbp). Loading: 5µL/lane.
・DNA Step Ladders ・OneSTEP Ladders ・Ladders for 10minute separation ・Wide Range Ladders ・Markers & OneSTEP Markers ・DGGE Markers ・PCR Marker Makers
Size St Standard Markers d ・RNA Si ・RNA Step Ladder ・Loading Buffers
Molecular-Weight Markers ・Prestain markers ・Western marker ・Biotinylated marker ・Markers for CBB dyeing
・Easy SeparatorTM ・Negative Gel Stain MS Kit ・SuperSepTM Ace ・SuperSepTM DNA
DNA Step Ladder (1~6)
Ready With dye and glycerol already to added, it is ready to be applied to use the agarose gel.
for low-molecular-range analysis.
100 90 80 70 60 50 40 30 20 10
6xBlue/ Orange Loading Dye
1% Agarose
Loading: 1µL/lane
1
(bp) −20℃ 300 275 250 225 200 175 150 125 100 Loading: 5µL/lane 75 50 25
−20℃
6x Loading Dye
Loading: 5µL/lane 2% Agarose
(bp)
−20℃
25 bp DNA Step Ladder (25-500 bp)
2
3
50 bp DNA Step Ladder (50-800 bp) (bp)
(bp) (ng) −20℃ 500 60 400 60 300 60 6xBlue/ Orange 250 60 Loading 200 120 Dye 175 80 150 80 125 80 Loading: 100 120 5µL/lane 75 80 50 80 25 80
800 700 600 500 400 300 200 150 100 50
2% Agarose
25 bp DNA Step Ladder (25-300 bp)
3.5% Agarose
10 bp DNA Step Ladder (10-100 bp)
4
OneSTEP Ladder (7~12) for simultaneous measurement of DNA size and amount.
5% Agarose 21
6x Loading Dye
5
717 600 500 400
6
Loading: 5-10µL/lane
OneSTEP Ladder 500 (0.5-5 kbp) (bp) 5,000 4,500 4,000 3,500 3,000 2,500 2,000
Ready to use
6x Loading Dye
286 200 100
7
Loading: 5µL/lane
OneSTEP Ladder 1 kb (1-10 kbp)
9
each fragment: 50ng/5µL
1% Agarose S
1,000 500
10
8
Concentration: 0.69µg/5µL
(kbp)
Ready to use
10.0 9.0 8.0 7.0 6.0 5.0 4.0 3.0
1,500
50 100
OneSTEP Ladder Supercoiled Plasmid
(kbp)
Ready to use
Ready to use
Gene Ladder 100 (0.1-2 kbp) (bp) (ng/5µL)
Ready to use
2,000 100 1,500 75
10.0 9.0 8.0 7.0 6.0 5.0
−20℃
4.0
2.0
1.0
11
each fragment: 50ng/5µL
2
each fragment: 30ng/5µL
(bp) (ng/5µL) 2,000 100 1,800 90 1,600 80 1,400 70 1,200 60 1,000 100 900 45 800 40 700 35 600 30 500 50 400 20 300 15 200 10 100 10
Concentration: 0.755µg/5µL
2% Agarose S
100 75 50 35 25 20 15 10
OneSTEP Ladder 100 (0.1-2 kbp)
(bp) (ng/5µL) 2,000 100 1,500 75 1,000 50 900 45 800 40 700 35 600 30 500 75 400 22.5 450 20 350 17.5 300 15 250 37.5 200 10 150 7.5 100 10
Ready to use
3% Agarose 21
200 150
−20℃
(bp) 1,500 1,000 900 800
−20℃
1% Agarose S
(bp) 300
Ready to use
OneSTEP Ladder 50 (0.05-2 kbp)
3% Agarose 21
100 bp DNA Step Ladder (0.1-1.5 kbp)
2% Agarose
DNA Step Ladder (10-300 bp)
1% Agarose S
DNA Ladder
DNA Ladder
3.0
12
1,000 900 800 700 600 500 400 300 200 100
Concentration: 0.64µg/5µL
50 45 40 35 30 75 40 30 20 100
Ladder for 10minute separation (13~18) (bp)
Ready to use
−20℃
(bp)
Ready to use
−20℃ 200 100
6x Loading Dye
50
(bp)
Ready to use
−20℃
5,000 2,000
1,500 6x Loading Dye
850
6x Loading Dye
850
DNA Step Ladder (500-10,000bp)
6x Loading Dye
Loading: 20µL/lane
14
100
25 25 25 25
0.1µg/5µL
Loading: 20µL/lane
15
2,000 1,000 500 100
17 Concentration:
2% Agarose
2% Agarose
50
(bp) (ng/5µL)
1,000 500
200
Ready to use
10,000 4,000 2,000
−20℃
400
400
10
2% Agarose
4% Agarose 21
Gene Ladder Fast 1 (4 bands:0.1-2 kbp)
(bp)
Ready to use
20
Loading: 20µL/lane
13
DNA Step Ladder (100-5,000 bp)
1% Agarose S
DNA Step Ladder (50-1,500 bp)
Loading: 20µL/lane
16
Gene Ladder Fast 2 (6 bands:0.1-10 kbp) Ready to use
1% Agarose S
DNA Step Ladder (10-200 bp)
−20℃ : Store at ー20℃ NO DISPLAY: Store at 2∼10℃
(bp) (ng/5µL) 10,000 4,000 2,000 1,000 500 100
25 25 25 25 25 25
18 Concentration: 0.15µg/5µL
Number
Product name
Code No.
Package Size
1
10 bp DNA Step Ladder (10-100 bp)
040-28721
32.5 μg (50 μL)
2
25 bp DNA Step Ladder (25-300 bp)
292-69001
100 μg
3
25 bp DNA Step Ladder (25-500 bp)
044-30711
500 μL
4
50 bp DNA Step Ladder (50-800 bp)
544-02311
85 μg (250 μL)
5
DNA Step Ladder(10-300 bp)
298-66301
50 μg
6
100 bp DNA Step Ladder(0.1-1.5 kbp)
296-68801
30 μg
7
8
9
OneSTEP Ladder 50(0.05-2.0 kbp)
OneSTEP Ladder 100(0.1-2 kbp)
OneSTEP Ladder 500 (0.5-5 kbp)
319-06044
25 μL
315-06041
500 μL
311-06043
500 μL ×2
317-05244
25 μL
313-05241
500 μL
319-05243
500 μL ×2
317-05364
25 μL
313-05361
500 μL
319-05363
500 μL × 3
314-05374
25 μL
310-05371
500 μL
10
OneSTEP Ladder 1 kb(1-10 kbp)
316-05373
500 μL × 3
11
OneSTEP Ladder Supercoiled Plasmid
317-05381
500 μL
12
Gene Ladder 100(0.1-2 kbp)
316-06951
500 μL
13
DNA Step Ladder(10-200 bp) , Fast Separation Type
292-66201
10 μg(0.5mL)× 2
14
DNA Step Ladder(50-1,500 bp) , Fast Separation Type
290-61001
10 μg(0.5mL)× 2
15
DNA Step Ladder(100-5,000 bp) , Fast Separation Type
296-61101
10 μg(0.5mL)× 2
16
DNA Step Ladder(500-10,000 bp) , Fast Separation Type
292-61201
10 μg(0.5mL)× 2
17
Gene Ladder Fast 1 (4 bands:0.1-2 kbp)
317-06981
500 μL× 2
18
Gene Ladder Fast 2(6 bands:0.1-10 kbp)
314-06991
500 μL× 2
Note
: Nippon Gene s products
3
DNA Ladder for wide-range and simultaneous analysis l i off b both th DNA size i and d amount. t
Marker
1,500
32
1,030 900 800 700 600 500 400 300 200 100 80
200 180 160 140 120 200 80 60 40 20 16
−20℃
20
(bp) (ng/5µL)
Ready to use
20,000 100 10,000 50 7,000 35 5,000 75 4,000 40 3,000 30
(bp) (ng/5µL)
Ready to use
2,000 100 1,500 30 1,300 26 1,000 100
21
700 35 500 75 400 40 300 30 200 20 100 100
& the OneSTEP Marker
−20℃
Gene Ladder Wide 2 (0.1-20 kbp)
Ready to use
22
1% Agarose
1% Agarose
6x Loading Dye
Gene Ladder Wide 1 (0.1-20 kbp)
(bp) (ng/5µL) 10,000 100 8,000 80 6,000 60 5,000 50 4,000 40 3,000 30 2,500 25 2,000 20 1,500 15 1,000 100 800 80 600 60 400 40 200 20
Ready to use
1% Agarose S
(bp)(ng/20µL) 10,000 200 8,000 160 6,000 120 5,000 100 4,000 80 3,000 60 2,500 50 2,000 40
−20℃
19
Smart Ladder (0.2-10 kbp)
1% Agarose
DNA Step Ladder Mix (0.08-10 kbp)
20,000 10,000 7,000 5,000 4,000 3,000
100 50 35 75 40 30
2,000 1,500 1,000 900 800 700 600 500 400 200 100
100 30 50 45 40 35 30 75 40 20 100
(23~33)
DNA molec molecular-weight lar eight markers that set si size e standards for linear do double-strand ble strand DNA DNA. E Each One-Step Marker comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel. Marker 2 (λ/Hind Ⅲ・EcoRⅠ) (kbp) 23.13
(kbp) 21.23
9.42 6.56
5.15
4.36
4.27
Marker 3 (λ/Hind Ⅲ+λ/EcoRⅠ)
4.98 3.52 2.02
Marker 5 (φX174/Hinc Ⅱ)
1.38 0.95
25
0.83
Marker 6 (λ/StyⅠ)
(bp) 1,057 770 612 495 392 345 341 335 297 291 210 162
(kbp) 19.33
(bp) 1,353 1,078 872 603
26
(kbp)
(kbp) 165.65 56.74
165.65 50.31 41.77
7.74 6.22
23.45 21.10 17.70
4.26 3.47 2.69 1.88
28
1.49 0.93
29 4
310 281 271 234 194 118 72
Marker 8GT (T4 GT7 + T4 GT7/Bgl Ⅱ)
10.06
30
0.3% Agarose H
79
(kbp) 23.13 21.23 9.42 7.42 6.56 5.81 5.65 4.88 4.36 3.52 2.32 2.02
Marker 7GT (T4 GT7 + T4 GT7/BglⅠ)
0.3% Agarose H
27
1.58
1% Agarose S
24
1% Agarose S
1.91
1% Agaose S
23
1% Agarose S
2.32 2.02
Marker 4 (φX174/Hae Ⅲ)
3% Agarose 21
Marker 1 (λ/Hind Ⅲ)
3% Agarose 21
DNA Ladder
Wide Range Ladder der (19~22)
17.82 17.70 15.71 14.28 10.65 8.03 5.59 5.51
Marker 10 (pBR322/MspⅠ)
Number
32
Marker 11 (pUC19/MspⅠ) (bp) 622 527 404 307 242 238 217 201 190 180 160 160 147 147 123 110 90 76 67
(bp)
33
Product name
19
DNA Step Ladder Mix(0.08-10 kbp)
20
Smart Ladder (0.2-10 kbp)
21 22
Code No.
Package Size
292-68401
1 mL 25 μL
Gene Ladder Wide 1(0.1-20 kbp)
313-06961
500 μL × 2
Gene Ladder Wide 2(0.1-20 kbp)
310-06971
OneSTEP Marker 2 (lambda/ dⅢ+
d Ⅲ digest)
Marker 2 (lambda/
dⅢ+
d Ⅲ digest)
R I double digest) R I double digest)
OneSTEP Marker 3 (lambda/ d Ⅲ + lambda/ Marker 3 (lambda/
d Ⅲ + lambda/
R I digest mixture) R I digest mixture) Ⅲ digest)
26 Marker 4(phiX174/
c Ⅱ digest)
Ⅰdigest)
Marker 6(lambda/
Ⅰdigest)
Marker 7 GT Marker 8 GT OneSTEP Marker 9(phiX174/ Marker 9(phiX174/
fⅠdigest)
fⅠdigest)
80 μg
315-00566
80 μg × 5
314-05271
1500 μL
310-05273
1500 μL × 5
316-00574
80 μg
312-00576
80 μg × 5
318-05791
375 μL
314-05793
375 μL × 5
Ⅰdigest)
317-05803
375 μL × 5
OneSTEP Marker 11(pUC19/
Ⅰdigest)
Ⅰdigest)
15 μg 15 μg × 5
311-05281
1500 μL
317-05283
1500 μL × 5
313-00964
80 μg
319-00966
80 μg × 5
315-03981
10 μg
311-03983
10 μg × 5
312-03991
10 μg 10 μg × 5
318-05811
375 μL
314-05813
375 μL × 5 15 μg 15 μg × 5
315-05821
375 μL
311-05823
375 μL × 5
313-01503
33
15 μg × 5 375 μL
317-01501
Ⅰdigest)
15 μg
311-05801
310-01493
OneSTEP Marker 10(pBR322/
Marker 11(pUC19/
1500 μL × 5
319-00564
314-01491
32 Marker 10(pBR322/
311-05264
318-03993
31
120 μg × 5 1500 μL
318-00676
28
120 μg
317-05261
312-00674
c Ⅱ digest)
OneSTEP Marker 6(lambda/
1500 μL × 5
311-00666
27 Marker 5(phiX174/
314-05254
315-00664
Ⅲ digest)
OneSTEP Marker 5(phiX174/
1500 μL
312-00456
Note
500 μL × 2
310-05251 316-00454
OneSTEP Marker 4(phiX174/
30
OneSTEP Marker: Store at 2∼10℃
500 μL
Marker 1(lambda/
29
−20℃
317-03941
OneSTEP Marker 1(lambda/
25
Storage conditions DNA MW Marker:
313-03943
23
24
501 489 404 331 242 190 147 111 110
3% Agarose 21
(bp) 726 713 553 500 427 417 413 311 249 200 151 140 118 100 82 66 66 48
3% Agarose 21
31
3% Agarose 21
Marker 9 (φX174/HinfⅠ)
15 μg 15 μg × 5
312-05831
375 μL
318-05833
375 μL × 5
319-03261
15 μg
315-03263
15 μg × 5
: Nippon Gene s products
5
DNA Ladder DNA markers for DGGE (dena (denaturing aturing grad gradient gel electrophoresis) electrophoresis). A CG clamp is a added at the end of one side of each fragment to enable separation / inspection by DGGE.
1 2 3
34
65%
5
35
65%
8% Acrylamide
4
1 2 3 4 5 6 7 8 9 10
DGGE Marker Ⅳ (8 fragments)
Denaturant 50% −20℃
36
70%
Denaturant 20% −20℃ 1 2 3 4 5 6 7 8 9 10
37
45%
No. of fragments (Conc.) Application
5 (50ng/5µL) For bacterial 16S rDNA
10 (100ng/5µL) For bacterial 16S rDNA
10 (100ng/5µL) For bacterial 16S rDNA
Constant voltage Electrophoresis time, Temp.
120V 8 hours, 60℃
120V 8 hours, 60℃
50V 18 hours, 58℃
PCR Marker Maker (39~42)
DGGE Marker Ⅴ (9 fragments) Denaturant 20% −20℃ 1 2 3 4 5 6 7 8
38 50%
1 2 3 4 5 6 7 8 9
6% Acrylamide
Denaturant 35% −20℃
DGGE Marker Ⅲ (10 fragments)
7% Acrylamide
Denaturant 35% −20℃
DGGE Marker Ⅱ (10 fragments)
6% Acrylamide
DGGE MarkerⅠ (5 fragments)
8% Acrylamide
DNA Ladder
4~38) DGGE Marker (34~38
8 (80ng/5µL) 9 (50ng/5µL) For fungal bacteria16S rDNA For fungal bacteria 16S rDNA 50V 20 hours, 60℃
75V 16 hours, 60℃
−20℃
for preparing DNA size markers markers. Consisting of a mixt mixture of template DNA, primer, and reaction buffer, the reagent initiates a PCR with the simple addition of a Tag DNA polymerase “Gene Tag.” It can also be used as a positive control while the PCR is under way. (bp) 1057 770 612 495 392 345 341 335 297 291 210 162 79 Number 34
35
36
37
38
39 40 41 42
M
39
40
41
42
43
(bp)
0.5 µg M:Marker 5(φX174/ c Ⅱ) 3 µL 39:100L(101 bp, 200 bp, 299 bp, 398 bp, 502 bp) 3 µL 40:100H(601 bp, 702 bp, 800 bp, 906 bp, 1,005 bp) 3 µL 41:200U(101 bp, 299 bp, 502 bp, 702 bp, 906 bp) 3 µL 42:200E(200bp, 398 bp, 601 bp, 805 bp, 1,005 bp) 3 µL each 43:Mixture of products from a 100L & 100H PCR
1005 905 800 702 601 502 398 299 200 101
Product name DGGE MarkerⅠ(5 fragments)
DGGE Marker Ⅱ (10 fragments)
DGGE Marker Ⅲ (10 fragments)
DGGE Marker Ⅳ (8 fragments)
DGGE Marker Ⅴ (9 fragments) PCR Marker Maker 100L PCR Marker Maker 100L, Gene
attached
PCR Marker Maker 100H PCR Marker Maker 100H, Gene
attached
PCR Marker Maker 200U PCR Marker Maker 200U, Gene
attached
PCR Marker Maker 200E PCR Marker Maker 200E, Gene
attached
Code No. 318-06411
Package Size 30 μL(for 6 applications)
314-06413
150 μL(for 30 applications)
312-06414
150 μL × 5(for 150 applications)
311-06401
30 μL(for 6 applications)
317-06403
150 μL(for 30 applications)
315-06404
150 μL × 5(for 150 applications)
315-06921
30 μL(for 6 applications)
311-06923
150 μL(for 30 applications)
319-06924
150 μL × 5(for 150 applications)
312-06931
30 μL(for 6 applications)
318-06933
150 μL(for 30 applications)
316-06934
150 μL × 5(for 150 applications)
319-06941
30 μL(for 6 applications)
315-06943
150 μL(for 30 applications)
313-06944
150 μL × 5(for 150 applications)
317-03061
For 10 applications
314-03071
For 10 applications
311-03081
For 10 applications
318-03091
For 10 applications
318-03111
For 10 applications
315-03121
For 10 applications
312-03131
For 10 applications
319-03141
For 10 applications
Note
: Nippon Gene s products
6
RNA Ladder
RNA Ladder RNA Size Standard Marker (1~4) RNA molecular-weight markers prepared by in vitro transcription.
(k base) 9
−70℃
(base)
−70℃
1% formaldehyde Agarose
6 5 4
6,583 4,981 3,638
300
2,604
1.5 1
2
200
100
3
RNA Ladder (0.125-6 kb)
(base)
−80℃
1,000 750 500 400
5% polyacrylamide-urea
1
3 2.5 2
0.5
RNA Size Standard Marker Ⅳ (0.28-6.58 kb)
(base)
−70℃
6,000 4,000 3,000 2,000 1,500 1,000 500 250 125
1% formaldehyde Agarose
RNA Size Standard Marker Ⅲ (0.1-1 kb)
1,908
1% formaldehyde Agarose
RNA Size Standard Marker Ⅱ
1,383 955 623 281
4
RNA Step Ladder (0.1–1 kb) (5)
Loading Buffer
an RNA molecular-weight l l i ht marker k can b be di directly tl applied li d to native gel, denatured gel, or polyacrylamide gel.
A sample can be easily applied during DNA gel electrophoresis to effectively predict the migration point. a
b
c
d
(Store at room temp.)
e
f
a
b
c
d
e
f
RNA Step Ladder (0.1-1 kb) Xylene Cyanol FF
(base)
2X Loading Dye
1,000 800 600
1,000 800 600
5
400 300 200 100
Number
2% Formaldehyde Agarose
(base)
2% Native Agarose
−80℃
Bromophenol Blue Orange G
400 300
a b c d e f
200 100
Marker 4 (φX173/Hae Ⅲ) Product PCR (299 bp) 50 ng TE Buffer
1 % agarose S 2 % agarose S 3 % agarose 21
Product name
6 × Loading Buffer Triple Dye 6 × Loading Buffer Orange G 6 × Loading Buffer Triple Dye 6 × Loading Buffer Orange G 6 × Loading Buffer Triple Dye 6 × Loading Buffer Orange G
Orange G Bromophenol Blue Xylene Cyanol FF 100 bp (approx.) 900 bp (approx.) 5,000 bp (approx.) 30 bp (approx.) 300 bp (approx.) 1,500 bp (approx.) 10 bp (approx.) 100 bp (approx.) 500 bp (approx.)
Code No.
Package Size
1
RNA Size Standard Marker Ⅱ(0.5-9 kb)
542-00651
50 μg
2
RNA Size Standard Marker Ⅲ(0.1-1 kb)
545-01621
50 μg
3
RNA Size Standard Marker Ⅳ(0.28-6.58 kb)
188-01831
50 μL
311-06261
25 μL
317-06263
25 μL × 2
4
RNA Ladder(0.125-6 kb)
5
RNA Step Ladder(0.1-1 kb)
298-61301
40 μL × 5
−
Loading Buffer
313-90111
10 mL
−
6 × Loading Buffer OrangeG
317-90251
1 mL × 3
−
6 × Loading Buffer Double Dye
313-90351
1 mL × 3
−
6 × Loading Buffer Triple Dye
314-90261
1 mL × 3
Note
: Nippon Gene s products
7
Protein Size Marker
Protein Size Marker Prestained Marker (1, 2) : With dye combined in its recombinant protein content. protein G with the ability to combine with the Fc region Western Marker (3) : Contains of immunoglobulin. Reacts with both primary and secondary antibodies in a western blot. biotinylated protein. Capable of both chromogenetic and Biotinylated Marker (4) : Contains photogenetic detection by reacting with avidin HRP or avidin AP. (kDa)
−20℃
140
(kDa) 240 140
100
100
−20℃
70 50 40
−20℃
60 50 42 35
42
31 21
25
15
14
16
2
Prestained
79
50
15
1
150 120 100 80
25 20
20
(kDa)
70
35
30
(kDa)
7
3
PrestainedⅡ
4
Western
Biotinylated
Marker for CBB Dyeing (5~8) Optimized for CBB dyeing. Obtains sharp, uniform bands because of a reduction alkyl process. (kDa)
(kDa)
(kDa)
(kDa)
200
180 116 97 79
116
30
20 17 6.5 3.5
For low molecular range
79
42
42
30
30
20 14
17
42
42
5
79
30 20 14 6.5
6
7
For middle molecular range
For high molecular range
8
Wide range −20℃ : Store at ー20℃ NO DISPLAY: Store at 2∼10℃
Number
Wako Cat. No.
Package Size
1
WIDE-VIEW TM Prestained Protein Size Marker
Product name
230-02221
500 μL
2
WIDE-VIEW TM Prestained Protein Size Marker 2
239-02291
500 μL
3
WIDE-VIEW TM Western Size Marker
233-02211
250 μL
4
WIDE-VIEW TM Biotinylated Size Marker
233-02331
1.5 mL
5
Molecular Weight Marker, Low Range
294-63101
1 mL
6
Molecular Weight Marker, Middle Range
131-14511
1 mL
7
Molecular Weight Marker, High Range
134-14501
1 mL
8
Molecular Weight Marker, Wide Range
296-63301
1 mL
8
Easy SeparatorTM (Wako Catalog No.058-07681 (1 set ) ) Easy SeparatorTM is an electrophoresis tank specially designed for “SuperSep” products to enable easy setting and removal of the gel plate.
Features 1 Easy to operate. 2 Requires only a small amount (about 250-350ml) of
running buffer for electrophoresis. 3 Allows the gel plate to be removed without removing the
running buffer after electrophoresis has been completed.
Easy Set
Easy Remove
①Insert a gel.
②Turn the knobs toward you.
③Setting is completed.
Negative Gel Stain MS Kit
①Remove the cover ②Turn the knobs after electrophoresis away from you. has been completed.
③Remove the gel without removing the running buffer.
(Store at room temperature)
Protein bands separated by SDS/polyacrylamide gel electrophoresis (SDS-PAGE) can be visualized as stable, clear and transparent bands against the background of milky white gel stained by negative gel stain containing our improved imidazol derivative reagent in as little as 10 minutes. The staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues for the subsequent studies of protein such as sequencing and mass analysis of peptide.
Featureas
Procedure SDS-PAGE gel
1 Detectable limit of protein: 1 ng
Staining Sol. A Shake 5 Wash 5
2 Sensitive and Simple procedure with 2-step staining in
10 min. 10 sec. x 3
5~10 minutes 3 Optimized the staining intensity by either de-staining the
Staining Sol. B
excess stain or re-staining following de-stain of the gel.
Shake 10 60 sec. Wash 1 min. x 3
Mass Spectrometry
4 Applicable to proteome analysis by mass spectrometry
and sequencing Check staining intensity of the gel against a sheet of black paper
MALDI-TOF/MS analysis
SDS-PAGE analysis 1
2
3
a
Figure 1.
b
SDS-PAGE analysis of proteins using Negative Gel Stain MS Kit
c
d
a : rat phosphorylase (97k); b : bovine serum albumin (68k); c : hen egg white ovalbumin (45k); d : bovine carbonic anhydrase (31k). Lane 1: 50 ng; Lane 2: 100 ng; Lane 3: 150 ng
relative abundance
rat muscle glycogen phosphorylase (EC2.4.1.1)
Figure 2.
MALDI-TOF/MS of 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 rat phosphorylase m/z The band was excised and treated with trypsin. Following the in-gel digestion and preparation, the sample was analyzed on MALDI-TOF mass spectrometer. (These data were provided by Dr. Y. Wada at Osaka Medical Center, Japan.)
Product name
Wako Cat. No.
Package Size
293-57701
20 tests
Negative Gel Stain MS Kit Kit Contents: 1) Staining Solution A (1 × 500 mL); 2) Staining Solution B (1 × 500 mL); 3)De-staining Solution (1 × 500 mL)
9
Electrophoresis-related Products
Electrophoresis-related Products
Electrophoresis-related Products Electrophoresis-related Products
SuperSepTM Ace SuperSep™ Ace is a polyacryla polyacrylamide amide gel of the highest quality quality, designed to resolve the problems of existing products (SuperSep™ and SuperSep™ HG). Tris-glycine buffer is used in the gel buffer, similar to existing products, but because its pH is neutralized, the gel’s chemical storage stability is improved. Physical storage stability is also improved by using vacuum packaging. And because it does not contain SDS, it can be used in SDS-PAGE and Native-PAGE by changing the running buffer.
Features
13 well
17 well
t enien Conv er for stick el g n! catio i f i t n ide
1 Good separation and uniform bands 2 Can be used with a multi-channel pipette. 3 Reasonably priced and capable of long-term storage (for 6 months) 4 Box sticker allows easy identification of type of gel. 5 Comes in 13-well and 17-well types.
100 × 100 × 3(mm)
oved Impr ation r sepa center! e h t near
90 × 85 × 1(mm)
(kDa)
Product specifications Plate size Gel size
13 wells
17 wells
30 μL
25 μL
200 150
3.3 ∼ 6.5 μg
1.3 ∼ 3.9 μg
100 75
Number of wells Well volume Total amount of protein* (/well)
SuperSepTM
SuperSepTM Ace
*Indicates total amount of protein that can be clearly separated.
50
rm Unifo d! ban
37
① SuperSepTM: 10% 0
1.5
3
4.5
6
7.5
9
10.5
12 (hours) 25
TM
②SuperSep
Ace: 10%
20
(Store at 2∼10℃)
Product name
Wako Cat. No.
Package Size
SuperSepTMAce, 6%,
13well
195-15171
10 ea.
SuperSepTMAce, 6%,
17well
192-15181
10 ea.
SuperSepTMAce, 7.5%,
13well
198-14941
10 ea.
SuperSepTMAce, 7.5%,
17well
191-14931
10 ea.
SuperSepTMAce, 10%,
13well
195-14951
10 ea.
SuperSepTMAce, 10%,
17well
192-14961
10 ea.
SuperSepTMAce, 12.5%,
13well
199-14971
10 ea.
SuperSepTMAce, 12.5%,
17well
196-14981
10 ea.
SuperSepTMAce, 15%,
13well
193-14991
10 ea.
SuperSepTMAce, 15%,
17well
190-15001
10 ea.
SuperSepTMAce, 5-12%, 13well
199-15191
10 ea.
SuperSepTMAce, 5-12%, 17well
192-15201
10 ea.
SuperSepTMAce, 5-20%, 13well
197-15011
10 ea.
SuperSepTMAce, 5-20%, 17well
194-15021
10 ea.
SuperSepTMAce, 10-20%, 13well
191-15031
10 ea.
SuperSepTMAce, 10-20%, 17well
198-15041
10 ea.
SuperSepTMAce, 15-20%, 13well(Tricine Gel)
198-15301
10 ea.
10
SuperSepTM DNA (15%, 17 wells) (Wako Catalog No.190-15481 (10 ea.) )
(Store at 2∼10℃)
Polyacrylamide gel is used in the separation of low-molecular-weight DNA because it has a finer gel mesh structure than agarose. However, the DNA size will vary widely depending on the acrylamide concentration and the degree of cross-linkage of the gel. In SuperSepTM DNA, the acrylamide concentration and the degree of cross-linkage of the gel have been optimized for the separation of low-molecular-weight DNA. This product is therefore effective for the separation of samples with a DNA size from 20-200 bp.
Features
Product specifications
1 The acrylamide concentration, degree of
Plate size
cross-linkage, and number of wells are optimized for the electrophoresis of low-molecular-weight DNA.
100 × 100 × 3(mm) 90 × 85 × 1(mm)
Gel size
17 wells
Number of wells
2 Cleaner separation image than that obtained with
25 μL
Well volume
agarose gel. 3 Ideal for 20-200 bp separation. (bp)
(bp) 100 90 80 70 60 50 40 30 20
(bp)
(bp)
200 100 90 80 70 60 50
150
200 150
100
100
75
75
50
50
25
40 30
25 20 SuperSepTM DNA (15%, 17 well)
1% Agarose
2.5% Agarose
SuperSepTM DNA (15%, 17 well)
Example of electrophoresis 20-200 bp DNA was clearly separated by using SuperSepTM DNA and Tris-glycine buffer. PCR products under 200 bp can be used for separation from gel, especially when cloning small RNA. 30-200 bp DNA was clearly separated with TBE buffer. ① With Tris-Glycine running buffer (bp)
② With TBE running buffer (bp)
(bp)
(bp)
200 150
200 150
100 100 50 50 30 25
20
30 20
EtBr staining
25
EtBr staining
Staining Reagent Product name
Wako Cat. No.
Package Size
EtBr Solution
315-90051
10 mL
11
Selection Guide 【How to read the chart】 1 , p1 ● Number Reference page
【Display codes】 NO DISPLAY Store at 2∼10℃
−80℃ Store at or below ー80℃
−20℃ Store at or below ー20℃
−70℃ Store at or below ー70℃
DNA Ladders 10 bp
1 kbp
100 bp
100 kbp 200 kbp
10 kbp
1 , p2 ● 2 , p2 ● 3 , p2 ● 4 , p2 ● 5 , p2 ● 6 , p2 ● 7 , p2 ● 8 , p2 ●
9 , p2 ● 10 , p2 ● 11 , p2 ●
12 , p2 ● 13 , p3 ● 14 , p3 ● 15 , p3 ● 16 , p3 ● 17 , p3 ● 18 , p3 ● 19 , p4 ● 20 , p4 ● 21 , p4 ● 22 , p4 ● 23 , p4 ● 24 , p4 ● 25 , p4 ● 26 , p4 ● 27 , p4 ● 28 , p4 ● 29 , p4 ● 30 , p4 ● 31 , p5 ● 32 , p5 ● 33 , p5 ● 39 , p6 ● 40 , p6 ● 41 , p6 ● 42 , p6 ●
10 bp
1 kbp
100 bp
100 kbp 200 kbp
10 kbp
RNA Ladders 10 b
10000 b
1000 b
100 b
1 , p7 ● 2 , p7 ● 3 , p7 ● 4 , p7 ● 5 , p7 ●
10 b
10000 b
1000 b
100 b
Protein Size Markers 10 kDa
1000 kDa
100 kDa 1 , p8 ● 2 , p8 ● 3 , p8 ● 4 , p8 ● 5 , p8 ● 6 , p8 ● 7 , p8 ● 8 , p8 ●
10 kDa
100 kDa
1000 kDa
・This brochure may contain products that cannot be exported to your country due to regulations. ・Listed products are intended for laboratory research use only, and not to be used for drug, food or human use.
co.jp
08X05開01 09Y3IBF