Molecular-Weight Markers

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OneSTEP Ladder 500. (0.5-5 kbp). OneSTEP Ladder 1 kb. (1-10 kbp). OneSTEP Ladder. Supercoiled Plasmid. Gene Ladder 100. (0.1-2 kbp). Loading: 5µL/lane.
・DNA Step Ladders ・OneSTEP Ladders ・Ladders for 10minute separation ・Wide Range Ladders ・Markers & OneSTEP Markers ・DGGE Markers ・PCR Marker Makers

Size St Standard Markers d ・RNA Si ・RNA Step Ladder ・Loading Buffers

Molecular-Weight Markers ・Prestain markers ・Western marker ・Biotinylated marker ・Markers for CBB dyeing

・Easy SeparatorTM ・Negative Gel Stain MS Kit ・SuperSepTM Ace ・SuperSepTM DNA

DNA Step Ladder (1~6)

Ready With dye and glycerol already to added, it is ready to be applied to use the agarose gel.

for low-molecular-range analysis.

100 90 80 70 60 50 40 30 20 10

6xBlue/ Orange Loading Dye

1% Agarose

Loading: 1µL/lane

1

(bp) −20℃ 300 275 250 225 200 175 150 125 100 Loading: 5µL/lane 75 50 25

−20℃

6x Loading Dye

Loading: 5µL/lane 2% Agarose

(bp)

−20℃

25 bp DNA Step Ladder (25-500 bp)

2

3

50 bp DNA Step Ladder (50-800 bp) (bp)

(bp) (ng) −20℃ 500 60 400 60 300 60 6xBlue/ Orange 250 60 Loading 200 120 Dye 175 80 150 80 125 80 Loading: 100 120 5µL/lane 75 80 50 80 25 80

800 700 600 500 400 300 200 150 100 50

2% Agarose

25 bp DNA Step Ladder (25-300 bp)

3.5% Agarose   

10 bp DNA Step Ladder (10-100 bp)

4

OneSTEP Ladder (7~12) for simultaneous measurement of DNA size and amount.

5% Agarose 21

6x Loading Dye

5

717 600 500 400

6

Loading: 5-10µL/lane

OneSTEP Ladder 500 (0.5-5 kbp) (bp) 5,000 4,500 4,000 3,500 3,000 2,500 2,000

Ready to use

6x Loading Dye

286 200 100

7

Loading: 5µL/lane

OneSTEP Ladder 1 kb (1-10 kbp)

9

each fragment: 50ng/5µL

1% Agarose S

1,000 500

10

8

Concentration: 0.69µg/5µL

(kbp)

Ready to use

10.0 9.0 8.0 7.0 6.0 5.0 4.0 3.0

1,500

50 100

OneSTEP Ladder Supercoiled Plasmid

(kbp)

Ready to use

Ready to use

Gene Ladder 100 (0.1-2 kbp) (bp) (ng/5µL)

Ready to use

2,000 100 1,500 75

10.0 9.0 8.0 7.0 6.0 5.0

−20℃

4.0

2.0

1.0

11

each fragment: 50ng/5µL

2

each fragment: 30ng/5µL

(bp) (ng/5µL) 2,000 100 1,800 90 1,600 80 1,400 70 1,200 60 1,000 100 900 45 800 40 700 35 600 30 500 50 400 20 300 15 200 10 100 10

Concentration: 0.755µg/5µL

2% Agarose S

100 75 50 35 25 20 15 10

OneSTEP Ladder 100 (0.1-2 kbp)

(bp) (ng/5µL) 2,000 100 1,500 75 1,000 50 900 45 800 40 700 35 600 30 500 75 400 22.5 450 20 350 17.5 300 15 250 37.5 200 10 150 7.5 100 10

Ready to use

3% Agarose 21

200 150

−20℃

(bp) 1,500 1,000 900 800

−20℃

1% Agarose S

(bp) 300

Ready to use

OneSTEP Ladder 50 (0.05-2 kbp)

3% Agarose 21

100 bp DNA Step Ladder (0.1-1.5 kbp)

2% Agarose

DNA Step Ladder (10-300 bp)

1% Agarose S

DNA Ladder

DNA Ladder

3.0

12

1,000 900 800 700 600 500 400 300 200 100

Concentration: 0.64µg/5µL

50 45 40 35 30 75 40 30 20 100

Ladder for 10minute separation (13~18) (bp)

Ready to use

−20℃

(bp)

Ready to use

−20℃ 200 100

6x Loading Dye

50

(bp)

Ready to use

−20℃

5,000 2,000

1,500 6x Loading Dye

850

6x Loading Dye

850

DNA Step Ladder (500-10,000bp)

6x Loading Dye

Loading: 20µL/lane

14

100

25 25 25 25

0.1µg/5µL

Loading: 20µL/lane

15

2,000 1,000 500 100

17 Concentration:

2% Agarose

2% Agarose

50

(bp) (ng/5µL)

1,000 500

200

Ready to use

10,000 4,000 2,000

−20℃

400

400

10

2% Agarose

4% Agarose 21

Gene Ladder Fast 1 (4 bands:0.1-2 kbp)

(bp)

Ready to use

20

Loading: 20µL/lane

13

DNA Step Ladder (100-5,000 bp)

1% Agarose S

DNA Step Ladder (50-1,500 bp)

Loading: 20µL/lane

16

Gene Ladder Fast 2 (6 bands:0.1-10 kbp) Ready to use

1% Agarose S

DNA Step Ladder (10-200 bp)

−20℃       : Store at ー20℃ NO DISPLAY: Store at 2∼10℃

(bp) (ng/5µL) 10,000 4,000 2,000 1,000 500 100

25 25 25 25 25 25

18 Concentration: 0.15µg/5µL

Number

Product name

Code No.

Package Size

1

10 bp DNA Step Ladder (10-100 bp)

040-28721

32.5 μg (50 μL)

2

25 bp DNA Step Ladder (25-300 bp)

292-69001

100 μg

3

25 bp DNA Step Ladder (25-500 bp)

044-30711

500 μL

4

50 bp DNA Step Ladder (50-800 bp)

544-02311

85 μg (250 μL)

5

DNA Step Ladder(10-300 bp)

298-66301

50 μg

6

100 bp DNA Step Ladder(0.1-1.5 kbp)

296-68801

30 μg

7

8

9

OneSTEP Ladder 50(0.05-2.0 kbp)

OneSTEP Ladder 100(0.1-2 kbp)

OneSTEP Ladder 500 (0.5-5 kbp)

319-06044

25 μL

315-06041

500 μL

311-06043

500 μL ×2

317-05244

25 μL

313-05241

500 μL

319-05243

500 μL ×2

317-05364

25 μL

313-05361

500 μL

319-05363

500 μL × 3

314-05374

25 μL

310-05371

500 μL

10

OneSTEP Ladder 1 kb(1-10 kbp)

316-05373

500 μL × 3

11

OneSTEP Ladder Supercoiled Plasmid

317-05381

500 μL

12

Gene Ladder 100(0.1-2 kbp)

316-06951

500 μL

13

DNA Step Ladder(10-200 bp) , Fast Separation Type

292-66201

10 μg(0.5mL)× 2

14

DNA Step Ladder(50-1,500 bp) , Fast Separation Type

290-61001

10 μg(0.5mL)× 2

15

DNA Step Ladder(100-5,000 bp) , Fast Separation Type

296-61101

10 μg(0.5mL)× 2

16

DNA Step Ladder(500-10,000 bp) , Fast Separation Type

292-61201

10 μg(0.5mL)× 2

17

Gene Ladder Fast 1 (4 bands:0.1-2 kbp)

317-06981

500 μL× 2

18

Gene Ladder Fast 2(6 bands:0.1-10 kbp)

314-06991

500 μL× 2

Note

: Nippon Gene s products

3

DNA Ladder for wide-range and simultaneous analysis l i off b both th DNA size i and d amount. t

Marker

1,500

32

1,030 900 800 700 600 500 400 300 200 100 80

200 180 160 140 120 200 80 60 40 20 16

−20℃

20

(bp) (ng/5µL)

Ready to use

20,000 100 10,000 50 7,000 35 5,000 75 4,000 40 3,000 30

(bp) (ng/5µL)

Ready to use

2,000 100 1,500 30 1,300 26 1,000 100

21

700 35 500 75 400 40 300 30 200 20 100 100

& the OneSTEP Marker

−20℃

Gene Ladder Wide 2 (0.1-20 kbp)

Ready to use

22

1% Agarose

1% Agarose

6x Loading Dye

Gene Ladder Wide 1 (0.1-20 kbp)

(bp) (ng/5µL) 10,000 100 8,000 80 6,000 60 5,000 50 4,000 40 3,000 30 2,500 25 2,000 20 1,500 15 1,000 100 800 80 600 60 400 40 200 20

Ready to use

1% Agarose S

(bp)(ng/20µL) 10,000 200 8,000 160 6,000 120 5,000 100 4,000 80 3,000 60 2,500 50 2,000 40

−20℃

19

Smart Ladder (0.2-10 kbp)

1% Agarose

DNA Step Ladder Mix (0.08-10 kbp)

20,000 10,000 7,000 5,000 4,000 3,000

100 50 35 75 40 30

2,000 1,500 1,000 900 800 700 600 500 400 200 100

100 30 50 45 40 35 30 75 40 20 100

(23~33)

DNA molec molecular-weight lar eight markers that set si size e standards for linear do double-strand ble strand DNA DNA. E Each One-Step Marker comes with pre-added dye and glycerol, so they can be applied directly to the agarose gel. Marker 2 (λ/Hind Ⅲ・EcoRⅠ) (kbp) 23.13

(kbp) 21.23

9.42 6.56

5.15

4.36

4.27

Marker 3 (λ/Hind Ⅲ+λ/EcoRⅠ)

4.98 3.52 2.02

Marker 5 (φX174/Hinc Ⅱ)

1.38 0.95

25

0.83

Marker 6 (λ/StyⅠ)

(bp) 1,057 770 612 495 392 345 341 335 297 291 210 162

(kbp) 19.33

(bp) 1,353 1,078 872 603

26

(kbp)

(kbp) 165.65 56.74

165.65 50.31 41.77

7.74 6.22

23.45 21.10 17.70

4.26 3.47 2.69 1.88

28

1.49 0.93

29 4

310 281 271 234 194 118 72

Marker 8GT (T4 GT7 + T4 GT7/Bgl Ⅱ)

10.06

30

0.3% Agarose H

79

(kbp) 23.13 21.23 9.42 7.42 6.56 5.81 5.65 4.88 4.36 3.52 2.32 2.02

Marker 7GT (T4 GT7 + T4 GT7/BglⅠ)

0.3% Agarose H

27

1.58

1% Agarose S

24

1% Agarose S

1.91

1% Agaose S

23

1% Agarose S

2.32 2.02

Marker 4 (φX174/Hae Ⅲ)

3% Agarose 21

Marker 1 (λ/Hind Ⅲ)

3% Agarose 21

DNA Ladder

Wide Range Ladder der (19~22)

17.82 17.70 15.71 14.28 10.65 8.03 5.59 5.51

Marker 10 (pBR322/MspⅠ)

Number

32

Marker 11 (pUC19/MspⅠ) (bp) 622 527 404 307 242 238 217 201 190 180 160 160 147 147 123 110 90 76 67

(bp)

33

Product name

19

DNA Step Ladder Mix(0.08-10 kbp)

20

Smart Ladder (0.2-10 kbp)

21 22

Code No.

Package Size

292-68401

1 mL 25 μL

Gene Ladder Wide 1(0.1-20 kbp)

313-06961

500 μL × 2

Gene Ladder Wide 2(0.1-20 kbp)

310-06971

OneSTEP Marker 2 (lambda/ dⅢ+

d Ⅲ digest)

Marker 2 (lambda/

dⅢ+

d Ⅲ digest)

R I double digest) R I double digest)

OneSTEP Marker 3 (lambda/ d Ⅲ + lambda/ Marker 3 (lambda/

d Ⅲ + lambda/

R I digest mixture) R I digest mixture) Ⅲ digest)

26 Marker 4(phiX174/

c Ⅱ digest)

Ⅰdigest)

Marker 6(lambda/

Ⅰdigest)

Marker 7 GT Marker 8 GT OneSTEP Marker 9(phiX174/ Marker 9(phiX174/

fⅠdigest)

fⅠdigest)

80 μg

315-00566

80 μg × 5

314-05271

1500 μL

310-05273

1500 μL × 5

316-00574

80 μg

312-00576

80 μg × 5

318-05791

375 μL

314-05793

375 μL × 5

Ⅰdigest)

317-05803

375 μL × 5

OneSTEP Marker 11(pUC19/

Ⅰdigest)

Ⅰdigest)

15 μg 15 μg × 5

311-05281

1500 μL

317-05283

1500 μL × 5

313-00964

80 μg

319-00966

80 μg × 5

315-03981

10 μg

311-03983

10 μg × 5

312-03991

10 μg 10 μg × 5

318-05811

375 μL

314-05813

375 μL × 5 15 μg 15 μg × 5

315-05821

375 μL

311-05823

375 μL × 5

313-01503

33

15 μg × 5 375 μL

317-01501

Ⅰdigest)

15 μg

311-05801

310-01493

OneSTEP Marker 10(pBR322/

Marker 11(pUC19/

1500 μL × 5

319-00564

314-01491

32 Marker 10(pBR322/

311-05264

318-03993

31

120 μg × 5 1500 μL

318-00676

28

120 μg

317-05261

312-00674

c Ⅱ digest)

OneSTEP Marker 6(lambda/

1500 μL × 5

311-00666

27 Marker 5(phiX174/

314-05254

315-00664

Ⅲ digest)

OneSTEP Marker 5(phiX174/

1500 μL

312-00456

Note

500 μL × 2

310-05251 316-00454

OneSTEP Marker 4(phiX174/

30

OneSTEP Marker:  Store at 2∼10℃

500 μL

Marker 1(lambda/

29

−20℃

317-03941

OneSTEP Marker 1(lambda/

25

Storage conditions DNA MW Marker: 

313-03943

23

24

501 489 404 331 242 190 147 111 110

3% Agarose 21

(bp) 726 713 553 500 427 417 413 311 249 200 151 140 118 100 82 66 66 48

3% Agarose 21

31

3% Agarose 21

Marker 9 (φX174/HinfⅠ)

15 μg 15 μg × 5

312-05831

375 μL

318-05833

375 μL × 5

319-03261

15 μg

315-03263

15 μg × 5

: Nippon Gene s products

5

DNA Ladder DNA markers for DGGE (dena (denaturing aturing grad gradient gel electrophoresis) electrophoresis). A CG clamp is a added at the end of one side of each fragment to enable separation / inspection by DGGE.

1 2 3

34

65%

5

35

65%

8% Acrylamide

4

1 2 3 4 5 6 7 8 9 10

DGGE Marker Ⅳ (8 fragments)

Denaturant 50% −20℃

36

70%

Denaturant 20% −20℃ 1 2 3 4 5 6 7 8 9 10

37

45%

No. of fragments (Conc.) Application

5 (50ng/5µL) For bacterial 16S rDNA

10 (100ng/5µL) For bacterial 16S rDNA

10 (100ng/5µL) For bacterial 16S rDNA

Constant voltage Electrophoresis time, Temp.

120V 8 hours, 60℃

120V 8 hours, 60℃

50V 18 hours, 58℃

PCR Marker Maker (39~42)

DGGE Marker Ⅴ (9 fragments) Denaturant 20% −20℃ 1 2 3 4 5 6 7 8

38 50%

1 2 3 4 5 6 7 8 9

6% Acrylamide

Denaturant 35% −20℃

DGGE Marker Ⅲ (10 fragments)

7% Acrylamide

Denaturant 35% −20℃

DGGE Marker Ⅱ (10 fragments)

6% Acrylamide

DGGE MarkerⅠ (5 fragments)

8% Acrylamide

DNA Ladder

4~38) DGGE Marker (34~38

8 (80ng/5µL) 9 (50ng/5µL) For fungal bacteria16S rDNA For fungal bacteria 16S rDNA 50V 20 hours, 60℃

75V 16 hours, 60℃

−20℃

for preparing DNA size markers markers. Consisting of a mixt mixture of template DNA, primer, and reaction buffer, the reagent initiates a PCR with the simple addition of a Tag DNA polymerase “Gene Tag.” It can also be used as a positive control while the PCR is under way. (bp) 1057 770 612 495 392 345 341 335 297 291 210 162 79 Number 34

35

36

37

38

39 40 41 42

M

39

40

41

42

43

(bp)

0.5 µg M:Marker 5(φX174/ c Ⅱ) 3 µL 39:100L(101 bp, 200 bp, 299 bp, 398 bp, 502 bp) 3 µL 40:100H(601 bp, 702 bp, 800 bp, 906 bp, 1,005 bp) 3 µL 41:200U(101 bp, 299 bp, 502 bp, 702 bp, 906 bp) 3 µL 42:200E(200bp, 398 bp, 601 bp, 805 bp, 1,005 bp) 3 µL each 43:Mixture of products from a 100L & 100H PCR

1005 905 800 702 601 502 398 299 200 101

Product name DGGE MarkerⅠ(5 fragments)

DGGE Marker Ⅱ (10 fragments)

DGGE Marker Ⅲ (10 fragments)

DGGE Marker Ⅳ (8 fragments)

DGGE Marker Ⅴ (9 fragments) PCR Marker Maker 100L PCR Marker Maker 100L, Gene

attached

PCR Marker Maker 100H PCR Marker Maker 100H, Gene

attached

PCR Marker Maker 200U PCR Marker Maker 200U, Gene

attached

PCR Marker Maker 200E PCR Marker Maker 200E, Gene

attached

Code No. 318-06411

Package Size 30 μL(for 6 applications)

314-06413

150 μL(for 30 applications)

312-06414

150 μL × 5(for 150 applications)

311-06401

30 μL(for 6 applications)

317-06403

150 μL(for 30 applications)

315-06404

150 μL × 5(for 150 applications)

315-06921

30 μL(for 6 applications)

311-06923

150 μL(for 30 applications)

319-06924

150 μL × 5(for 150 applications)

312-06931

30 μL(for 6 applications)

318-06933

150 μL(for 30 applications)

316-06934

150 μL × 5(for 150 applications)

319-06941

30 μL(for 6 applications)

315-06943

150 μL(for 30 applications)

313-06944

150 μL × 5(for 150 applications)

317-03061

For 10 applications

314-03071

For 10 applications

311-03081

For 10 applications

318-03091

For 10 applications

318-03111

For 10 applications

315-03121

For 10 applications

312-03131

For 10 applications

319-03141

For 10 applications

Note

: Nippon Gene s products

6

RNA Ladder

RNA Ladder RNA Size Standard Marker (1~4) RNA molecular-weight markers prepared by in vitro transcription.

(k base) 9

−70℃

(base)

−70℃

1% formaldehyde Agarose

6 5 4

6,583 4,981 3,638

300

2,604

1.5 1

2

200

100

3

RNA Ladder (0.125-6 kb)

(base)

−80℃

1,000 750 500 400

5% polyacrylamide-urea

1

3 2.5 2

0.5

RNA Size Standard Marker Ⅳ (0.28-6.58 kb)

(base)

−70℃

6,000 4,000 3,000 2,000 1,500 1,000 500 250 125

1% formaldehyde Agarose

RNA Size Standard Marker Ⅲ (0.1-1 kb)

1,908

1% formaldehyde Agarose

RNA Size Standard Marker Ⅱ

1,383 955 623 281

4

RNA Step Ladder (0.1–1 kb) (5)

Loading Buffer

an RNA molecular-weight l l i ht marker k can b be di directly tl applied li d to native gel, denatured gel, or polyacrylamide gel.

A sample can be easily applied during DNA gel electrophoresis to effectively predict the migration point. a

b

c

d

(Store at room temp.)

e

f

a

b

c

d

e

f

RNA Step Ladder (0.1-1 kb) Xylene Cyanol FF

(base)

2X Loading Dye

1,000 800 600

1,000 800 600

5

400 300 200 100

Number

2% Formaldehyde Agarose

(base)

2% Native Agarose

−80℃

Bromophenol Blue Orange G

400 300

a b c d e f

200 100

Marker 4 (φX173/Hae Ⅲ) Product PCR (299 bp) 50 ng TE Buffer

1 % agarose S 2 % agarose S 3 % agarose 21

Product name

6 × Loading Buffer Triple Dye 6 × Loading Buffer Orange G 6 × Loading Buffer Triple Dye 6 × Loading Buffer Orange G 6 × Loading Buffer Triple Dye 6 × Loading Buffer Orange G

Orange G Bromophenol Blue Xylene Cyanol FF 100 bp (approx.) 900 bp (approx.) 5,000 bp (approx.) 30 bp (approx.) 300 bp (approx.) 1,500 bp (approx.) 10 bp (approx.) 100 bp (approx.) 500 bp (approx.)

Code No.

Package Size

1

RNA Size Standard Marker Ⅱ(0.5-9 kb)

542-00651

50 μg

2

RNA Size Standard Marker Ⅲ(0.1-1 kb)

545-01621

50 μg

3

RNA Size Standard Marker Ⅳ(0.28-6.58 kb)

188-01831

50 μL

311-06261

25 μL

317-06263

25 μL × 2

4

RNA Ladder(0.125-6 kb)

5

RNA Step Ladder(0.1-1 kb)

298-61301

40 μL × 5



Loading Buffer

313-90111

10 mL



6 × Loading Buffer OrangeG

317-90251

1 mL × 3



6 × Loading Buffer Double Dye

313-90351

1 mL × 3



6 × Loading Buffer Triple Dye

314-90261

1 mL × 3

Note

: Nippon Gene s products

7

Protein Size Marker

Protein Size Marker Prestained Marker (1, 2) : With dye combined in its recombinant protein content. protein G with the ability to combine with the Fc region Western Marker (3) : Contains of immunoglobulin. Reacts with both primary and secondary antibodies in a western blot. biotinylated protein. Capable of both chromogenetic and Biotinylated Marker (4) : Contains photogenetic detection by reacting with avidin HRP or avidin AP. (kDa)

−20℃

140

(kDa) 240 140

100

100

−20℃

70 50 40

−20℃

60 50 42 35

42

31 21

25

15

14

16

2

Prestained

79

50

15

1

150 120 100 80

25 20

20

(kDa)

70

35

30

(kDa)

7

3

PrestainedⅡ

4

Western

Biotinylated

Marker for CBB Dyeing (5~8) Optimized for CBB dyeing. Obtains sharp, uniform bands because of a reduction alkyl process. (kDa)

(kDa)

(kDa)

(kDa)

200

180 116 97 79

116

30

20 17 6.5 3.5

For low molecular range

79

42

42

30

30

20 14

17

42

42

5

79

30 20 14 6.5

6

7

For middle molecular range

For high molecular range

8

Wide range −20℃       : Store at ー20℃ NO DISPLAY: Store at 2∼10℃

Number

Wako Cat. No.

Package Size

1

WIDE-VIEW TM Prestained Protein Size Marker

Product name

230-02221

500 μL

2

WIDE-VIEW TM Prestained Protein Size Marker 2

239-02291

500 μL

3

WIDE-VIEW TM Western Size Marker

233-02211

250 μL

4

WIDE-VIEW TM Biotinylated Size Marker

233-02331

1.5 mL

5

Molecular Weight Marker, Low Range

294-63101

1 mL

6

Molecular Weight Marker, Middle Range

131-14511

1 mL

7

Molecular Weight Marker, High Range

134-14501

1 mL

8

Molecular Weight Marker, Wide Range

296-63301

1 mL

8

Easy SeparatorTM (Wako Catalog No.058-07681 (1 set ) ) Easy SeparatorTM is an electrophoresis tank specially designed for “SuperSep” products to enable easy setting and removal of the gel plate.

Features 1 Easy to operate. 2 Requires only a small amount (about 250-350ml) of

running buffer for electrophoresis. 3 Allows the gel plate to be removed without removing the

running buffer after electrophoresis has been completed.

Easy Set

Easy Remove

①Insert a gel.

②Turn the knobs toward you.

③Setting is completed.

Negative Gel Stain MS Kit

①Remove the cover ②Turn the knobs after electrophoresis away from you. has been completed.

③Remove the gel without removing the running buffer.

(Store at room temperature)

Protein bands separated by SDS/polyacrylamide gel electrophoresis (SDS-PAGE) can be visualized as stable, clear and transparent bands against the background of milky white gel stained by negative gel stain containing our improved imidazol derivative reagent in as little as 10 minutes. The staining technique is useful to obtain the clear and sensitive resolution pattern of the gel before immunoblotting as well as to excise and purify the band of interest from the gel without significant deterioration of amino acid residues for the subsequent studies of protein such as sequencing and mass analysis of peptide.

Featureas

Procedure SDS-PAGE gel

1 Detectable limit of protein: 1 ng

Staining Sol. A Shake 5 Wash 5

2 Sensitive and Simple procedure with 2-step staining in

10 min. 10 sec. x 3

5~10 minutes 3 Optimized the staining intensity by either de-staining the

Staining Sol. B

excess stain or re-staining following de-stain of the gel.

Shake 10 60 sec. Wash 1 min. x 3

Mass Spectrometry

4 Applicable to proteome analysis by mass spectrometry

and sequencing Check staining intensity of the gel against a sheet of black paper

MALDI-TOF/MS analysis

SDS-PAGE analysis 1

2

3

a

Figure 1.

b

SDS-PAGE analysis of proteins using Negative Gel Stain MS Kit

c

d

a : rat phosphorylase (97k); b : bovine serum albumin (68k); c : hen egg white ovalbumin (45k); d : bovine carbonic anhydrase (31k). Lane 1: 50 ng; Lane 2: 100 ng; Lane 3: 150 ng

relative abundance

rat muscle glycogen phosphorylase (EC2.4.1.1)

Figure 2.

MALDI-TOF/MS of 1000 1100 1200 1300 1400 1500 1600 1700 1800 1900 2000 rat phosphorylase m/z The band was excised and treated with trypsin. Following the in-gel digestion and preparation, the sample was analyzed on MALDI-TOF mass spectrometer. (These data were provided by Dr. Y. Wada at Osaka Medical Center, Japan.)

Product name

Wako Cat. No.

Package Size

293-57701

20 tests

Negative Gel Stain MS Kit Kit Contents: 1) Staining Solution A (1 × 500 mL); 2) Staining Solution B (1 × 500 mL); 3)De-staining Solution (1 × 500 mL)

9

Electrophoresis-related Products

Electrophoresis-related Products

Electrophoresis-related Products Electrophoresis-related Products

SuperSepTM Ace SuperSep™ Ace is a polyacryla polyacrylamide amide gel of the highest quality quality, designed to resolve the problems of existing products (SuperSep™ and SuperSep™ HG). Tris-glycine buffer is used in the gel buffer, similar to existing products, but because its pH is neutralized, the gel’s chemical storage stability is improved. Physical storage stability is also improved by using vacuum packaging. And because it does not contain SDS, it can be used in SDS-PAGE and Native-PAGE by changing the running buffer.

Features

13 well

17 well

t enien Conv er for stick el g n! catio i f i t n ide

1 Good separation and uniform bands 2 Can be used with a multi-channel pipette. 3 Reasonably priced and capable of long-term storage (for 6 months) 4 Box sticker allows easy identification of type of gel. 5 Comes in 13-well and 17-well types.

100 × 100 × 3(mm)

oved Impr ation r sepa center! e h t near

90 × 85 × 1(mm)

(kDa)

Product specifications Plate size Gel size

13 wells

17 wells

30 μL

25 μL

200 150

3.3 ∼ 6.5 μg

1.3 ∼ 3.9 μg

100 75

Number of wells Well volume Total amount of protein* (/well)

SuperSepTM

SuperSepTM Ace

*Indicates total amount of protein that can be clearly separated.

50

rm Unifo d! ban

37

① SuperSepTM: 10% 0

1.5

3

4.5

6

7.5

9

10.5

12 (hours) 25

TM

②SuperSep

Ace: 10%

20

(Store at 2∼10℃)

Product name

Wako Cat. No.

Package Size

SuperSepTMAce, 6%,

13well

195-15171

10 ea.

SuperSepTMAce, 6%,

17well

192-15181

10 ea.

SuperSepTMAce, 7.5%,

13well

198-14941

10 ea.

SuperSepTMAce, 7.5%,

17well

191-14931

10 ea.

SuperSepTMAce, 10%,

13well

195-14951

10 ea.

SuperSepTMAce, 10%,

17well

192-14961

10 ea.

SuperSepTMAce, 12.5%,

13well

199-14971

10 ea.

SuperSepTMAce, 12.5%,

17well

196-14981

10 ea.

SuperSepTMAce, 15%,

13well

193-14991

10 ea.

SuperSepTMAce, 15%,

17well

190-15001

10 ea.

SuperSepTMAce, 5-12%, 13well

199-15191

10 ea.

SuperSepTMAce, 5-12%, 17well

192-15201

10 ea.

SuperSepTMAce, 5-20%, 13well

197-15011

10 ea.

SuperSepTMAce, 5-20%, 17well

194-15021

10 ea.

SuperSepTMAce, 10-20%, 13well

191-15031

10 ea.

SuperSepTMAce, 10-20%, 17well

198-15041

10 ea.

SuperSepTMAce, 15-20%, 13well(Tricine Gel)

198-15301

10 ea.

10

SuperSepTM DNA (15%, 17 wells) (Wako Catalog No.190-15481 (10 ea.) )

(Store at 2∼10℃)

Polyacrylamide gel is used in the separation of low-molecular-weight DNA because it has a finer gel mesh structure than agarose. However, the DNA size will vary widely depending on the acrylamide concentration and the degree of cross-linkage of the gel. In SuperSepTM DNA, the acrylamide concentration and the degree of cross-linkage of the gel have been optimized for the separation of low-molecular-weight DNA. This product is therefore effective for the separation of samples with a DNA size from 20-200 bp.

Features

Product specifications

1 The acrylamide concentration, degree of

Plate size

cross-linkage, and number of wells are optimized for the electrophoresis of low-molecular-weight DNA.

100 × 100 × 3(mm) 90 × 85 × 1(mm)

Gel size

17 wells

Number of wells

2 Cleaner separation image than that obtained with

25 μL

Well volume

agarose gel. 3 Ideal for 20-200 bp separation. (bp)

(bp) 100 90 80 70 60 50 40 30 20

(bp)

(bp)

200 100 90 80 70 60 50

150

200 150

100

100

75

75

50

50

25

40 30

25 20 SuperSepTM DNA (15%, 17 well)

1% Agarose

2.5% Agarose

SuperSepTM DNA (15%, 17 well)

Example of electrophoresis 20-200 bp DNA was clearly separated by using SuperSepTM DNA and Tris-glycine buffer. PCR products under 200 bp can be used for separation from gel, especially when cloning small RNA. 30-200 bp DNA was clearly separated with TBE buffer. ① With Tris-Glycine running buffer (bp)

② With TBE running buffer (bp)

(bp)

(bp)

200 150

200 150

100 100 50 50 30 25

20

30 20

EtBr staining

25

EtBr staining

Staining Reagent Product name

Wako Cat. No.

Package Size

EtBr Solution

315-90051

10 mL

11

Selection Guide 【How to read the chart】 1 , p1 ● Number Reference page

【Display codes】 NO DISPLAY Store at 2∼10℃

−80℃ Store at or below ー80℃

−20℃ Store at or below ー20℃

−70℃ Store at or below ー70℃

DNA Ladders 10 bp

1 kbp

100 bp

100 kbp 200 kbp

10 kbp

1 , p2 ● 2 , p2 ● 3 , p2 ● 4 , p2 ● 5 , p2 ● 6 , p2 ● 7 , p2 ● 8 , p2 ●

9 , p2 ● 10 , p2 ● 11 , p2 ●

12 , p2 ● 13 , p3 ● 14 , p3 ● 15 , p3 ● 16 , p3 ● 17 , p3 ● 18 , p3 ● 19 , p4 ● 20 , p4 ● 21 , p4 ● 22 , p4 ● 23 , p4 ● 24 , p4 ● 25 , p4 ● 26 , p4 ● 27 , p4 ● 28 , p4 ● 29 , p4 ● 30 , p4 ● 31 , p5 ● 32 , p5 ● 33 , p5 ● 39 , p6 ● 40 , p6 ● 41 , p6 ● 42 , p6 ●

10 bp

1 kbp

100 bp

100 kbp 200 kbp

10 kbp

RNA Ladders 10 b

10000 b

1000 b

100 b

1 , p7 ● 2 , p7 ● 3 , p7 ● 4 , p7 ● 5 , p7 ●

10 b

10000 b

1000 b

100 b

Protein Size Markers 10 kDa

1000 kDa

100 kDa 1 , p8 ● 2 , p8 ● 3 , p8 ● 4 , p8 ● 5 , p8 ● 6 , p8 ● 7 , p8 ● 8 , p8 ●

10 kDa

100 kDa

1000 kDa

・This brochure may contain products that cannot be exported to your country due to regulations. ・Listed products are intended for laboratory research use only, and not to be used for drug, food or human use.

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08X05開01 09Y3IBF