MOXIFLOXACIN LOADED SOLID LIPID NANOPARTICLES (SLN) FOR TOPICAL DELIVERY PREPARATION AND CHARACTERIZATION R. Naveen chakravarthi*1, Dr. S.K. Umadevi1, Dr. K. Jayaraj kumar1, P.Nithyaswi1, B. Lakshmi Narasamma1, S. Suresh1, M.R. Thiruganesh1, V. Shanmugam2. 1. Department of Pharmaceutics, Rao’s college of pharmacy, Nellore, AP. 2. Department of pharmaceutics, Sri Padmavathi School of Pharmacy, Tirupati, A.P. e-mail:
[email protected]
Abstract The aim of present work was to formulate Moxifloxacin loaded solid lipid nanoparticles (SLNS) using, palmitic acid (4.5%) w/v as a lipophilic material, Tween 80 (4% w/v), poloxamer (4% w/v) as surfactants and methanol, butanol (each 4% w/v) as cosolvents. The SLNs were prepared by o/w microemulsion technique which involves high speed homogenization. The obtained Nanodispersions were allowed to cool to form SLN. The SLN which shows good physical stability was selected and it was characterized for particle size analysis, zeta potential, drug content, entrapment efficiency, Transmission Electron Microscopy (TEM) photographs, Differential scanning calorimetry (DSC) and IR studies. Results indicated mixed lipid-matrix, produced nanosuspensions with low-crystallinity, smaller particle sizes, no drug-excipient incompatibility and higher drug entrapment. The optimized formulation was incorporated into various gels like carbopol (1% w/v) and xanthan gum gel (0.5 % w/v). Preliminary physical parameters of gels like viscosity, gel strength were evaluated. In vitro drug release studies were carried out for moxifloxacin SLN loaded gel preparations and % drug loading capacity was calculated. Ex vivo penetration studies were conducted for 12 hrs using Keshary Chien diffusion cell. The studies revealed a stable formulation without precipitation of drug and a sustained drug release from the gel matrix. SLN composed of moxifloxacin would prove to be a good topical drug delivery in treating bacterial infections. Keywords: Moxifloxacin, poloxamer, zeta potential, carbopol, xanthan gum, nanosuspensions.
