NH4:NO3 nutrition influence on biomass productivity

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The solution was adjusted with NaOH or HCl to pH 5.0±0.5. Element Treatment Chemical compounds. Concentration. (µM). N. NH4Cl and NaNO3 *. 2000.00‡.
NH4:NO3 nutrition influence on biomass productivity and root respiration of poplar and willow clones Boris Rewald*, Martin E. Kunze, Douglas L. Godbold Forest Ecology, Dept. of Forest and Soil Sciences, University of Natural Resources and Life Sciences Vienna (BOKU), Peter-Jordan-Straße 82, 1190 Vienna, Austria

*Corresponding author. Phone: +43147654-4140, Fax: -4129, Email: [email protected]

Supporting information

Table S.1 Composition of the used nutrient solution (Ingestad & Lund, 1979, Woolfolk & Friend, 2003) for plant fertilisation and as solution during the respiration measurements (see text). The solution was adjusted with NaOH or HCl to pH 5.0±0.5. Element

Treatment

Chemical compounds

N

Concentration (µM) 2000.00‡

NH4Cl and NaNO3 * 50.0% 50.0% NH4Cl – 50.0% NaNO3 62.5% 47.5% NH4Cl – 62.5% NaNO3 75.0% 25.0% NH4Cl – 75.0% NaNO3 87.5% 12.5% NH4Cl – 87.5% NaNO3 K KCl 460.00 P KH2PO4 120.00 Mg MgSO4 90.00 Ca CaCl2 60.00 B H3BO3 5.30 † Fe Fe(NO3)3 3.56 Mn MnCl2 2.08 Zn Zn(NO3)2† 0.26 Cu CuCl2 × 2H2O 0.13 Co CoCl2 0.03 Mo Na2MoO4 0.04 + *The proportion of NH4 and NO3 was varied, based on molar weights, according to treatment. ‡ The maximum applied NH4+ concentration is 1 mM, the NO3- concentrations lay between 1-2 mM and are within the common range of soil solutions (Marschner, 1997, Owen & Jones, 2001). † Total NO3- concentration is not significantly affected by these concentrations. NO3NO3NO3NO3-

Reference: Ingestad T, Lund AB (1979) Nitrogen stress in birch seedlings. I. Growth techniqueand growth. Physiologia Plantarum, 45, 137–148.

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