Annexure.1. GC-MS CHROMATOGRAM- INTERPRETATION FOR THE PEAKS OF THE SAMPLES: Samples: MS-1 and XC-1 are sample codes for the FAME from Myxosarcina sp. for GC-MS analysis. Unidentified peaks: Represented as NI- non identified peaks (Retention time – 10.7, 14.6, 15.12, 15.45, 17.82) (marked in the chromatogram attached below this document) NIIST Database matches: Untransesterified fatty acids such as Octadecene and Eicosene, corresponding to the retention time of 13.77and 15.88 respectively were also detected in the chromatogram (Mass spectrum data- Annexure. 3) #NOTE: Hence both the retention time of Supelco standard and NIIST database were used to interpret the compounds. The following table reveals the interpretation of the peaks of the chromatogram of the sample.
NI NI
NI
Fig.1. GC-MS chromatogram showing each peaks and NI denotes non identified peaks
Table.1. Retention times, m/z value, carbon number and identification of each peak by comparing with NIIST database and Supelco standard. Retention time
Compound
m/Z
Carbon no.
Compared to
10.7
NI
NI
NI
NI
13.0
Methyl tetradecanoate
242
C14:0
Both Supelco and database
13.77
3 - Octadecene
252
C18:1
NIIST database
14.6
NI
NI
NI
NI
15.02
Hexadecenoic acid methyl ester
268
C16:1
Supelco standard
15.27
Hexadecanoic acid methyl ester
270
C16:0
Both Supelco and database
15.88
9- Eicosene
280
C20:2
NIIST database
17.02
8 - Octadecenoic methyl ester
296
C18:1
Both Supelco and database
17.22
Octadecanoic methyl ester
298
C18:0
Both Supelco and database
17.82
NI
NI
NI
Table.2. Properties of sugar industry waste (SIW). Parameters Values Total dissolved solids (TDS) Reducing sugars Protein pH
Fig.2. FT-IR analysis of SIW sample with evidence of organic nitrogen and carbon compounds (Shakambari Ganeshan et al., 2015).
Reference: 1. John Coates. Encyclopedia of Analytical Chemistry, 2000. Interpretation of Infrared Spectra, A Practical Approach. R.A. Meyers (Ed.). pp. 10815–10837. Ó John Wiley & Sons Ltd, Chichester
2. Shakambari Ganeshan, Sumi M Babu, Ashokkumar Balasubramaniem, Palanivelu Peramachi and Varalakshmi Perumal. Industrial effluent as a substrate for glutaminase free L-asparaginase production from Pseudomonas plecoglossicida strain RS1; media optimization, enzyme purification and its characterization, RSC Advances, 2015, DOI: 10.1039/C5RA05507E.
