Articles in PresS. Am J Physiol Heart Circ Physiol (February 21, 2003). 10.1152/ajpheart.00360.2001
NOS Inhibition Accelerates Atherogenesis: Reversal by Exercise Josef Niebauer, MD, PhD Andrew J. Maxwell, MD Patrick S. Lin, BS David Wang, BS
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Philip S. Tsao, PhD 1 John P. Cooke, MD, PhD 1
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Section of Vascular Medicine, Divisions of Cardiovascular Medicine, Stanford University,
CA 2
Herzzentrum der Universität Leipzig, Kardiologie, Leipzig, Germany
Short title: Niebauer et al. Exercise reverses NOS inhibition
Address for correspondence:
John P. Cooke MD, PhD Director, Vascular Medicine Falk Cardiovascular Research Center Stanford University School of Medicine 300 Pasteur Drive Stanford, CA 94305 - 5246 USA phone: (650) 725-3778 fax: (650) 725-1599 email:
[email protected]
Copyright (c) 2003 by the American Physiological Society.
ABSTRACT Background In this study we assessed the effects of chronic exercise training (12 weeks) on atherosclerotic lesion formation in hypercholesterolemic apolipoprotein-E deficient mice (n=31). Methods and Results At the age of 9 weeks mice were assigned to the following groups: sedentary (Sed; n=9); exercise (Ex; n=12); sedentary and oral L-nitroarginine (SedNA; n=4), or exercise and oral L-nitroarginine (Ex-NA; n=6). Chronic exercise training was performed on a treadmill for 12 weeks, 6x/week, 2x1hr/d, at a final speed of 22m/min, and an 8° grade. LNA was discontinued 5 days prior to final treadmill testing. The farthest distance run to exhaustion was observed in Ex-NA mice (Sed: 306±32 m; Ex: 640±87; Sed-NA: 451±109 m; Ex-NA: 820 ±49 m; all p
