Novel Antibiofilm Chemotherapy Targets Exopolysaccharide ...

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Aug 21, 2012 - The use of natural products in the treatment and prevention of dental caries is an ... using selected naturally occurring food-derived agents (myricetin and tt-farnesol) ..... Amira 5.4.1 software (Visage Imaging, San. Diego, CA) ...
Novel Antibiofilm Chemotherapy Targets Exopolysaccharide Synthesis and Stress Tolerance in Streptococcus mutans To Modulate Virulence Expression In Vivo Megan L. Falsetta,a Marlise I. Klein,a José A. Lemos,a,b Bruno B. Silva,a,c Senyo Agidi,a Kathy K. Scott-Anne,a and Hyun Kooa,b Center for Oral Biology, University of Rochester Medical Center, Rochester, New York, USAa; Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, New York, USAb; and Piracicaba Dental School, University of Campinas–UNICAMP, Department of Physiologic Science, Piracicaba, Sao Paulo, Brazilc

Fluoride is the mainstay of dental caries prevention, and yet current applications offer incomplete protection and may not effectively address the infectious character of the disease. Therefore, we evaluated the effectiveness of a novel combination therapy (CT; 2 mM myricetin, 4 mM tt-farnesol, 250 ppm of fluoride) that supplements fluoride with naturally occurring, food-derived, antibiofilm compounds. Treatment regimens simulating those experienced clinically (twice daily for