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SHIFTING OF PEA PROTEIN ISOLATE ENHANCES INTESTINAL ABSORPTION. OF VITAMIN D IN RATS. Ali M. Almajwal. 1. , Mahmoud M. A. Abulmeaty. * 1, 2.
NOVEL VITAMIN D-NANOEMULSION DEVELOPED BY SONICATION AND PHSHIFTING OF PEA PROTEIN ISOLATE ENHANCES INTESTINAL ABSORPTION OF VITAMIN D IN RATS 1 Almajwal ,

* 1, 2 Abulmeaty ,

3 Andrade , Mohamed

4 Elsadek ,

MONLB273 4 Razak

Ali M. Mahmoud M. A. Juan E. F. Suhail and project no: 12-NAN 2576-02 funded by the national plan of science, technology and innovation, King AbdulAziz City for Science & Technology (KACST) 1Department

of Community health sciences, Clinical nutrition program, King Saud University, Riyadh, Saudi Arabia, 2Obesity research and management unit, Medical Physiology Department, Faculty of Medicine, Zagazig University , Zagazig , Egypt, 3Department of Food Science and Human Nutrition, University of Illinois, Urbana-Champaign, United States, 4community health sciences, clinical nutrition program, KSU, Riyadh, Saudi Arabia

Introduction: Limited dispersion, instability, and bioavailability of Vitamin D (VD) requires improved formulation. In this study, the absorptive ability of a novel VD (cholecalciferol) containing nanoemulsion (VDN) developed by sonication and pH-shifting of pea protein isolate and canola oil vs. a commercial oral VD form in rats was evaluated. Methods: Twenty-four, adult male albino rats (289±43 g) were housed in single cages and fed AIN-93M diets ad libitum. All single oral doses were delivered by gavage. The four treatment groups (n=6 rats each) were:

a) a 3 ml of VDN solution containing 27 ug/ml VD (3,240 IU), b) the nanoemulsion without VD, c) the same VD dose using the commercial VD with 3 ml of canola oil, and d) a 3 ml dose of canola oil without VD. Serial blood samples (n=10) were automatically withdrawn (0, 0.5, 1, 2, 4, 6, 8, 10, 12, and 24 h) from surgically inserted carotid catheter in each rat using an automated blood 1 sampling device . The zero time represents the basal level after recovery from surgery and just before ingestion of treatment. Plasma 25-hydroxy vitamin D (25OHVD) was measured by 2 ELISA .

Results: At all time-points, VDN treatment showed higher levels 25OHVD compared to the commercial formulation or controls (P0.05

Acknowledgment: this project is funded by the national plan of science, technology and innovation, King Abdul-Aziz City for Science & Technology (KACST) project no: 12-NAN 2576-02

ESPEN2016

References: 1 Teilmann et al. Manual versus automated blood sampling: impact of repeated blood sampling on stress parameters and behavior in male NMRI mice. Lab Anim. 2014; 48(4): 278–291. 2 Kir et al. Effects of carbamazepine on serum parathormone, 25-hydroxyvitamin D, bone specific alkaline phosphatase, C-telopeptide, and osteocalcin levels in healthy rats. Bosn J Basic Med Sci. 2012 November; 12(4): 240–244. 273--MON-LB

Nutritional techniques and formulations Mahmoud ABULMEATY

DOI: 10.3252/pso.eu.ESPEN2016.2016

Poster presented at: