40. UO.614. LP1.60h..014 UpI. Op 0. 61. SO h.1.LP0. 61. 1.H1.kII Up0. 614 00. 0.1.. .C . 040...0..O . 0.0 . 06~ÃT.C .T..6 aC.4... R. ..0. C. T.0..M. ..UT.0.CWT.. C.4.
Nucleic Acids Research
Volume 17 Number 4 1989
Nucleotide sequence of the Candida albicans aspartyl proteinase gene
T.J.Lott*, L.S.Page, P.Boiron , J.Bensont and E.Reiss Division of Mycotic Diseases, Building 5/B12 G- 1I, 'Division of Host Factors, Centers for Disease Control, 1600 Clifton Road, Aflanta, GA 30333, USA and 2MyCology Unit, Pasteur Institute, France EMBL accession no. X13669 Submitted December 7, 1988
An EMBL3 clone containing the -C. alb.icAns aspartyl proteinase (PrA) gene has been identified through hybridization with a S.. jmexyialiae ErA probe. We report here the sequence of the C. tsa.14an gene and flanking regions. NUcleotide sequence conservation between the yeast and.C. .aJbLcAn genes (1) is 72% within the structural gene. The yeast sequence is given above, where dots represent identical nucleotides. There is an 85% homology at the amino acid level, and the overall codon bias is similar (2). However, the NM2 terminal signal peptide (3) of 5.. *r.eWiau is Completely different than the amino terminus of C. ..a2b.ican , suggesting a different targeting of these analogous proteins. In addition there is a 37 amino acid open reading frame which terminates immediately adjacent to the first initiation codon of thefjC). gene (beginning at np 521). Upstream from the small open reading frame is an AfT rich region, typical of yeast pronotors. Studies are in progress to determine the possible e expression of this gene in.fi. .rxMjpr 0.0 00S..0. 0..L.0.-01 0..TOoII
Lo101
II.1.
TW101.L.o 01.L.. II M0.0.
. 0.I..0.0. 61loTO
Ni .-0o0.6
pT.04
.
.
o O
11.lC1 0.1
. 1.OOV . Tp014.0 RBG MUp UpTOo
.
UT UT UTT UTC noU TOO UTTOT MC UT
Hat..01 o
01 0T h 0040040R TT
..4.0.0. 4.64
.o0.Co 0 ol Lvrr
0.01OpCCr 01 ItO 11. 01. Up 0.1 L. 1. . . 01CTTCT 0..rg 010 0...0...0...614.Op C
0. N
A11.01.0..C0, Op90 0.I . TOprTT 61RTC40To oo4M p
FLo 01.
. 0 0.H. 1 220oL.o0
0 no UT T UTC UCC
TO.
001. 0
.Lo .O.U.
61TTTTUpL.o00
6.0..01L.o0'.0,61 260
1
. 0.0. Up1C1 0 ER1.
1
1.4..0401
UT UCT UTE UT UT TC UNT UWT UMFTU
p0'
pLo
1.6.01
RT CAR04 UT UT UT UTrT
060 0 1 0.O . 0. 0. U Up014l 00. 0.Up614 Op f 0..h 0..614 01al604aL.oAl.1. Al01. Lo.pSUpAl1. -Ty L.ooy0 1..61t4 iO-
UT no UT UT UT UT UT UTC UT UTB UTUT UT 1. T0gLop 0 A.Op Top ROI.. Topi.o40, A 1Ph1.b
0
UTC
MET0
no UT UT UTC 00M UTU TUTUTY 040 UTUTnoU UT UT UT UTUT 00 URT UTUT UTC UT no 40. UO. 614 P1.60 014 UpI. Op 0. 61. SO h.1. LP0. 61 1. H1.kII Up0. 614 00. 0.1.. h.. L
.C . 040...0..O . 0.0 . 06~~~~OT.C .T..6 aC.4... ..0C R. . T.0..M . ..UT .0.C WT.. C.4.. . ..0 n TUnnF TU TU UTU iU 4 UTC UTU cU MTT 000C UT UT UT UT OCCr UT UT ono UT UTUT UTac no40U T TUT noI UTT 1 . o1. 1 UpUp lop Up 1. Lop0.r TV- L- 604 RI. Op. 6y 00.hTr1. - l 40. UPrPh.o00 Al. 01. Vl. P0. 0h. 0.l T..o 00. 01. 1 1. T604 ly 0.r yo
Oo,Ri,P
. 00 . T . . ... ... ..C TUT..T..1 .40.0.0T..0.T ..A..T U ..R..RUTC. --T40.04 noU oU TU U fT UT UT UT 006 UTM UT U .T ..T 000 UT06C 0 TU oU 0U. T00.T0040U .COU TUT UTTOU 00 01. 614 Op0.r4ly 00r .o 00. 40. Op Upl B 0. 614 0 I.Cop Ph. 0. M0.1 Al. 000 40.. 0.0 p W. 40M RI. 40.r Sr 614 Up, L.o A1. hr0. olo S O-S 6.p Op
L4 MTiU iC -T i UTU
p
U
0 l.a1 IV
I=~ 0TO
Sgx
al.
OUC T
400004
O W 1 1 ..C OTc OU a nwr TU TUT Tn UT no' UT UT UTi UT UT 00 --T C UT UT UTf 6000000110 00M000040609000300 Op yUp U Hi. Lop 0..004p0.1614 I. 01. r 40. 0. Lop ft1 1. l l A h l 1 I r r1. p
OUT-
TT T 01 n UT U TCUTU 01 40r L.o8. V0 Lop Op Opr 0.y Ph
ett 1r I.Woo. l h.Ap l C1.A., A. lford,yLy O
h
al 1.SrVlA i.(96MolVP-P-I.
3..Klons.yD..MJM , et.al (188 .olT © IRLPress
h
10T0TU0T4I0 UT
A
Cell. Biol.. sl
Cell. Bio.TFI. A.8
21052116
IB 34 17794 Ia 10
