Jan 10, 2003 - independent of PLC (Agam, K., von Campenhausen, M.,. Levy, S., Ben-Ami, H. C., Cook, B., Kirschfeld, K., and. Minke, B. (2000) J. Neurosci.
THE JOURNAL OF BIOLOGICAL CHEMISTRY © 2003 by The American Society for Biochemistry and Molecular Biology, Inc.
Vol. 278, No. 21, Issue of May 23, pp. 18851–18858, 2003 Printed in U.S.A.
Rescue of Light Responses in the Drosophila “Null” Phospholipase C Mutant, norpAP24, by the Diacylglycerol Kinase Mutant, rdgA, and by Metabolic Inhibition* Received for publication, January 10, 2003, and in revised form, February 27, 2003 Published, JBC Papers in Press, March 5, 2003, DOI 10.1074/jbc.M300310200
Roger C. Hardie‡, Fernando Martin, Sylwester Chyb§, and Padinjat Raghu¶ From the Cambridge University Department of Anatomy, Cambridge CB2 3DY, United Kingdom
Light responses in Drosophila are reportedly abolished in severe mutants of the phospholipase C (PLC) gene, norpA. However, on establishing the whole-cell recording configuration in photoreceptors of the supposedly null allele, norpAP24, we detected a small (⬃15 pA) inward current that represented spontaneous light channel activity. The current decayed during ⬃20 min, after which tiny residual responses (
