Oligonucleotides used in this study Construct Name Oligonucleotides ...

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Dec 5, 2013 - ∆2. 5'UTR-sml-. Del. GGGGGGGCATGCTGGATTATTGCTAACCCAGCAC. AG. ClaI-Umut CCCCCCATCGATGGATATATATACACTTTGCC. ∆3.
Oligonucleotides used in this study Construct

Name

Oligonucleotides (5’-3’)

1

Site 6F 5’

GAGTCTAAACAAGGGGAGC

P2D1Rev

GGGGGCATGCTATATATCATGTGATATGTTC

2

5’UTR-sml- GGGGGGGCATGCTGGATTATTGCTAACCCAGCAC Del AG ClaI-Umut

CCCCCCATCGATGGATATATATACACTTTGCC

3

DelSphIRBS

GGGGGGGCATGCTAATTATAAGGGAAAAACGAT GAAG x ClaI-Umut

OLE

RfaHPutOP-fw

GGGGGGCATGCAAGAGCATCCGCTATCAATGGCA ACGTTATTATAATTAACAG x ClaI-Umut

rfaH complement

rfaHlacUV5new

GGGGGGATCCTCTTAATCTGCGCCCATTATACGG CG

lacUV5rfaH_down

GGGGAAGCTTGGCTGGCTGCCACCACGGATG

rfaH_fw

GCCTGGCACCGATGATCACCC

rfaH_rv

GGAGCGAGCCTCACCATCGGG

Sequencing OLE

SphI-fw

GTCATATGTGAGACGGCTAGTTGAACG x ClaIUmut

OLE transcriptional fusion

Opstranscript

GGGGGATCCCCTAGTCTAGACTAGCGATGGATAT ATATACACTTTGCCTTAAGATCAATATCCG x RfaH-PutOP-fw

micA-lacZYA upstream

micA-upxbaI

GGGGTCTAGAATAACAAATGCGCGTCTTTCATAT ACTCAGACTCGCCTG

HindIIIluxS

GGGGAAGCTTTACGCGAACGCTGGCGG

micAdown-xbaI

GGGGTCTAGACATCATCCCTGAATTCAGAGATGA AATTTTGG

KpnI-gshA

GGGGGGTACCGATCTTTACGAGTACGTAGCGGG

Lac-

GGGGAAGCTTACGGGCAGACATGGCCTGCCCGG

rfaH allele check

micA-lacZYA downstream

micA-lacZYA

orientation check of lacZYA

(HindIII)down

x HindIII-luxS

lacZ-upPstI-AM

GGGGGGCTGCAGCCCACAGCCGCCAGTTCCGC x KpnI-gshA