Optimized Detection Method of Telomerase Activity in ... - Springer Link

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INTRODUCTION. Telomerase is an RNA–protein complex, which is composed of an RNA component (a template for telomere synthesis), which has a structural ...

ISSN 00271314, Moscow University Chemistry Bulletin, 2010, Vol. 65, No. 3, pp. 165–169. © Allerton Press, Inc., 2010. Original Russian Text © D.A. Skvortsov, M.E. Zvereva, M.P. Rubtsova, L.S. Pavlova, A.A. Petrenko, F.L. Kisseljov, O.A. Dontsova, 2010, published in Vestnik Moskovskogo Universiteta. Khimiya, 2010, No. 3, pp. 197–202.

Optimized Detection Method of Telomerase Activity in Cancer Diagnostics D. A. Skvortsova, M. E. Zverevaa, M. P. Rubtsovaa, L. S. Pavlovab, A. A. Petrenkob, F. L. Kisseljovb, and O. A. Dontsovaa a

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Chair of Chemistry of Natural Compounds, Faculty of Chemistry, Moscow State University, Moscow, Russia Research Institute of Carcinogenesis, Cancer Research Center, Russian Academy of Medical Sciences, Moscow, Russia email: [email protected] Received January 20, 2010

Abstract—Telomerase is a ribonucleoprotein complex; it uses an internal RNA template to synthesize telom ere DNA. Telomerase is active in 90% of cancers and can be used as a diagnostic marker. We have optimized conditions for the extraction from small tissue samples (

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