Oxidation of Aliphatic Olefins by Toluene Dioxygenase: Enzyme Rates

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present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, ... a rate of 33 nmol per min per mg of protein. Products from enzyme reactions were identified by gas .... Chemical shifts for.
JOURNAL OF BACTERIOLOGY, June 1997, p. 3858–3865 0021-9193/97/$04.0010 Copyright © 1997, American Society for Microbiology

Vol. 179, No. 12

Oxidation of Aliphatic Olefins by Toluene Dioxygenase: Enzyme Rates and Product Identification CLESTON C. LANGE

AND

LAWRENCE P. WACKETT*

Department of Biochemistry, Biological Process Technology Institute, and Center for Biodegradation Research and Informatics, Gortner Laboratory, University of Minnesota, St. Paul, Minnesota 55108 Received 16 December 1996/Accepted 14 April 1997

Toluene dioxygenase from Pseudomonas putida F1 has been studied extensively with aromatic substrates. The present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene as substrates for toluene dioxygenase. Enzyme specific activities were determined for the more water-soluble C2 to C5 compounds and ranged from