present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, ... a rate of 33 nmol per min per mg of protein. Products from enzyme reactions were identified by gas .... Chemical shifts for.
Oxidation of Aliphatic Olefins by Toluene Dioxygenase: Enzyme Rates and Product Identification CLESTON C. LANGE
AND
LAWRENCE P. WACKETT*
Department of Biochemistry, Biological Process Technology Institute, and Center for Biodegradation Research and Informatics, Gortner Laboratory, University of Minnesota, St. Paul, Minnesota 55108 Received 16 December 1996/Accepted 14 April 1997
Toluene dioxygenase from Pseudomonas putida F1 has been studied extensively with aromatic substrates. The present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene as substrates for toluene dioxygenase. Enzyme specific activities were determined for the more water-soluble C2 to C5 compounds and ranged from