Papaya (Carica papaya) leaf methanolic extract ...

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Animal Science Journal (2016) ••, ••–••

doi:10.1111/asj.12634

ORIGINAL ARTICLE Papaya (Carica papaya) leaf methanolic extract modulates in vitro rumen methanogenesis and rumen biohydrogenation Saeid JAFARI,1 Yong M. GOH,1,2 Mohamed A. RAJION,1 Mohammad F. JAHROMI,2 Yusof H. AHMAD1 and Mahdi EBRAHIMI1 1

Department of Veterinary Preclinical Sciences, Faculty of Veterinary Medicine, and 2Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia

ABSTRACT Papaya leaf methanolic extract (PLE) at concentrations of 0 (CON), 5 (LLE), 10 (MLE) and 15 (HLE) mg/250 mg dry matter (DM) with 30 mL buffered rumen fluid were incubated for 24 h to identify its effect on in vitro ruminal methanogenesis and ruminal biohydrogenation (BH). Total gas production was not affected (P > 0.05) by addition of PLE compared to the CON at 24 h of incubation. Methane (CH4) production (mL/250 mg DM) decreased (P < 0.05) with increasing levels of PLE. Acetate to propionate ratio was lower (P