4 '. 1996. Phosphodiesterase. Inhibitors Correct Resistance to .... The resistance is not related to an alteration ..... Model 943, Lexington,. MA). Plasma and urine.
Phosphodiesterase Natriuretic Peptides Jean-Pierre William
Valentin,
G. Couser,
Inhibitors Correct Resistance in Rats with Heymann
Wei-Zhong and
Ving,
Michael
Leonardo
A. Sechi,
W.-Z. Ying. K-I. phreys. Division of Nephrology. Hospital. University of California Francisco. CA
Ling. C. Qiu, M.H. HumSan Francisco General at San Francisco, San
Sechi, Medicine,
Department Udine, Italy
Hypertension Unit. University of Udine,
L.A.
Couser. Division of Washington. Seattle, WA
Nephrology,
W.G.
(J.
Am.
Soc.
Nephrol.
1996;
nephrotic
abnormal
sodium
of Internal
natriuretic infused
syndrome
University
of
response both atrial natriuretic
presented plasma
is characterized
reflected
to volume peptide
here examined ANP concentration
the
by
in a blunted
expansion (ANP). The
relationships and urinary
and to studies
among sodium
days
after ip injection of anti-Fx1A antiserum, rats were proteinuric and had a blunted natriuretic response to intravenous infusion of isotonic saline (2% body weight, given over 5 mm). Thirty mm after the onset of
nephritic
plasma the same
rats,
ANP concentration was inextent in both normal and
compared
with
their
respective
hydra-
penic controls. Despite this increase, UCGMPV was significantly less in nephritic rats after the saline infuslon. Accumulation of cGMP by isolated glomeruli and IMCD cells from nephritic rats after incubation with ANP and RNP was also significantly reduced,
compared with normal rats. This difference was not related to differences in either density or affinity of renal ANP receptors, but was abolished when accumulation of cGMP was measured in the presence of
1
Received
2 Portions
November of
this
1992;40:181A. 3
Correspondence
SFGH, University
15, 1995.
work
and
were
J Am Soc
Accepted
December
previously
published
Nephroi
1991 ;3:448A.
to Dr. M.H. Humphreys, of Caiifornia
at San Francisco,
in
Division
12, 1995. abstract
1046.6673/0704.0582$03.OO/O Journal of the American Society of Nephroiogy copyright © 1996 by the American Society of Nephrology
582
form
of Nephrology.
San Francisco,
(PDE).
in
din
Box CA 94143.
of cyclic
Infusion
nephritic
Qiu,
orZaprinast,
nucleotide
of Zaprinast
rats normalized
cGMP-PDE ±
32
protein,
