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Sep 26, 2008 - Address: 1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK and 2Department of Cell.
Plant Methods

BioMed Central

Open Access

Methodology

High-throughput Agrobacterium-mediated barley transformation Joanne G Bartlett1, Sílvia C Alves2, Mark Smedley1, John W Snape1 and Wendy A Harwood*1 Address: 1Department of Crop Genetics, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK and 2Department of Cell and Developmental Biology, John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK Email: Joanne G Bartlett - [email protected]; Sílvia C Alves - [email protected]; Mark Smedley - [email protected]; John W Snape - [email protected]; Wendy A Harwood* - [email protected] * Corresponding author

Published: 26 September 2008 Plant Methods 2008, 4:22

doi:10.1186/1746-4811-4-22

Received: 15 August 2008 Accepted: 26 September 2008

This article is available from: http://www.plantmethods.com/content/4/1/22 © 2008 Bartlett et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background: Plant transformation is an invaluable tool for basic plant research, as well as a useful technique for the direct improvement of commercial crops. Barley (Hordeum vulgare) is the fourth most abundant cereal crop in the world. It also provides a useful model for the study of wheat, which has a larger and more complex genome. Most existing barley transformation methodologies are either complex or have low (