Platelet-Activating Factor Mediates Phosphatidylcholine Hydrolysis by ...

BIOLOGY

OF

REPRODUCTION

59-65

47,

Platelet-Activating

(1992)

Factor

ASIF

Mediates Phosphatidylcholine in Human Endometrium1

AHMED,2’3

Department

RU!

of Obstetrics

and

of Pbysiology

ROBIN

Gynaecology,3

Robinson Department

FERRIANI,3

A.

Way,

& Pharmacology,4

PLEVIN,4

University

Cambridge

Hydrolysis

CB2

University

and

STEPHEN

of Cambridge, 25W,

by Phospholipase

K. SMITH3

Rosie

United

D

Maternity

Hospital

Kingdom

of Strathclyde,

Glasgow

G12

8QQ

United

Kingdom

ABSTRACT Human

preimplantation

choline

(PC)

preincubated

with

metrium and mm

stimulated

l3Hlbutan-1-ol.

demonstrated

that

had

13H]GPC pholipase

increased

the

to

activation. that

source

breakdown

water-soluble

(p

in

factor

endomethal of

(‘HiCho)

was

The

generation

is catalyzed synthesis

by

the

confirmed

precedes

by

the

of I5HIPBut,

with

PC

that

lmethyl.3H]choline

hydrolysis

of [3Hjcholine

in

or

secretory

endo-

(I

5H JChoP)

phosphate

D pathway. of PC in human

pathway

essential

generation

may

the

rise

it is proposed secretory

for long.term

that

PAF

by 2 Both

that PClysophos-

l#{176}H]butan-lol

in intracellular

[3HICho,

induces

endometrium. activation

suggest A, and

phospholipase

of high-specific-activity

paralleled

breakdown

an important

slower [5HICh0P due to PC-specific

incorporation which

phospholipase

D-catalyzed and

PAF-induced

of phosphatidyl-

mechanism

prelabeled

30 sec there was a rapid rise in PAF-induced [‘HiCho generation and 0.02), with no effect upon [5H]ChoP and 13H]GPC during this period.