Jed Y. J. Lee, Prof. Robert Hill. Institute of Dentistry www.smd.qmul.ac.uk. 1. Introduction. The need and demand for bone graft substitutes have been rising in the ...
Institute of Dentistry
Porous Bioactive Glass Scaffolds for Bone Regeneration Jed Y. J. Lee, Prof. Robert Hill
1. Introduction The need and demand for bone graft substitutes have been rising in the recent decades due to an increasing and ageing world population.
Currently, bone graft substitutes made from bioactive glasses are only commercially available in the particulate form and have not been made into porous bone scaffolds because these glass compositions crystallise easily during the sintering process required to produce the scaffold.
170 ºC
Optimal Firing Temperature 710 ºC
180 ºC
670 ºC
~99% Amorphous Adequately-Sintered
Mean Total Porosity
86.6 ± 2.6%
Pore Size
Mean Open Porosity
77.6 ± 9.4%
153–557 µm
In addition, there has been increasing interest in strontium incorporation into bioactive glasses because strontium has been shown to upregulate osteoblasts and downregulate osteoclasts, promoting bone formation.
2. Aims 1.To develop and characterise porous scaffolds of the all-calcium (SP-0Sr-35Ca) and all-strontium (SP-35Sr-0Ca) variants of Stronbone-P, a strontium-containing bioactive glass composition.
In Vitro Bioactivity Studies: • SP-0Sr-35Ca scaffolds showed clear signs of apatite-like phase development from day 3 onwards in both Tris buffer and SBF.
2.To assess the effect of full strontium substitution on the properties of the porous bioactive glass scaffolds.
• In contrast, SP-35Sr-0Ca scaffolds showed no clear signs of apatite-like phase development in both Tris buffer and SBF.
Analyse: • Degree of Sintering & Pore Structure by SEM • Crystallinity by XRD Assess In Vitro Bioactivity in Tris Buffer & SBF
Produce the Scaffold ‘Green Body’ by Polymer Foam Replication Technique Sinter Scaffolds at a Selected Firing Temperature
5. Conclusion 1. Porous scaffolds of the all-calcium (SP0Sr-35Ca) and all-strontium (SP-35Sr0Ca) variants of Stronbone-P have been successfully developed. 2. These scaffolds fulfilled the minimum requirements for supporting vascularised bone growth.1
Analyse for 3. Full strontium substitution inhibited the Surface Apatite formation of an apatite-like phase in Tris Formation by FTIR
Measure Total Porosity and Open Porosity
buffer and SBF, highlighting the importance of calcium in facilitating surface apatite formation.2
References: 1. Rahaman MN, Day DE, Bal BS, Fu Q, Jung SB, Bonewald LF, et al. Bioactive glass in tissue engineering. Acta Biomater. 2011;7(6):2355-73. 2. Sriranganathan D, Kanwal N, Hing KA, Hill RG. Strontium substituted bioactive glasses for tissue engineered scaffolds: the importance of octacalcium phosphate. J Mater Sci Mater Med. 2016;27(2):39.
