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Cartilage • Glycosaminoglycans • Oral supplementation • Osteochondral lesion. Introduction .... extrapolation from the standard curve with shark chondroitin ...
Physiol. Res. 56: 243-249, 2007

Positive Effect of Oral Supplementation with Glycosaminoglycans and Antioxidants on the Regeneration of Osteochondral Defects in the Knee Joint M. HANDL1, E. AMLER2,6, K. BRÄUN3, J. HOLZHEU3, T. TRČ1, A. B. IMHOFF3, A. LYTVYNETS4, E. FILOVÁ2,6, H. KOLÁŘOVÁ5, A. KOTYK4.V. MARTÍNEK3 1

Orthopedic Clinic, Charles University, Prague, Czech Republic, 2Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic, 3Department of Orthopedic Sports Medicine, Technical University Munich, Germany, 4Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic, 5Institute of Medical Biophysics, Medical Faculty, Palacký University in Olomouc, Czech Republic and 6Institute of Biophysics, Second Faculty of Medicine, Charles University in Prague, Czech Republic Received November 29, 2005 Accepted February 27, 2006 On-line available March 23, 2006

Summary The effect of oral supplementation with glycosaminoglycans (GAG) and radical scavengers (vitamin E/selenium) on the regeneration of osteochondral defects was investigated in rabbits. After introduction of defined osteochondral defects in the knee joint, groups of ten animals were given a GAG/vitamin E/selenium mixture or a placebo (milk sugar) for 6 weeks. Following sacrifice, histological and histochemical analysis was performed. The amount of synovial fluid was increased in the placebo group, while the viscosity of the synovial fluid was significantly enhanced in the GAG group. The amount of sulfated GAG in the osteochondral regenerates (8.8 ± 3.6 % vs. 6.0 ± 5.6 %; p