Poster Abstracts

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doi: 10.1111/j.1600-051x-2012.01891.x

Poster Abstracts

Topic: Aetiology and pathogenesis P0001 A Retrospective Analysis of the Etiology of PeriImplantitis and Implant Failure C.P. Hamm1, A. Piccini1, D.M. Lavoie1, S.M. Sabo1, L. Dakin1, W. Ward2, P.C. Fritz1 1Fonthill/Canada, 2St. Catharines/Canada

Aim: Peri-implantitis, a serious complication of implant dentistry, poses a challenging problem for the clinician. A major issue for the near future is the growing incidence of peri-implantitis. Published studies suggest 25-53% of patients with implants have peri-implantitis.. Peri-implantitis is a multifactorial disease with the etiology sharing many similarities with periodontitis including the need for maintenance for disease prevention. By developing a protocol to identify and predictably treat patients with peri-implant mucositis and implantitis, the patient benefits from improved peri-implant health. Material and Methods: As a referral center for patients with implant complications, we have developed a protocol and algorithm for the identification of peri-implant diseases as well the treatment of peri-implant mucositis and implantitis. A retrospective analysis of 25 consecutive patients with periimplantitis was undertaken. Patients were followed on average for 8.5 years.

and 2008, respectively. Recently, a clinical strain TDC60 was isolated and genome-sequenced in Japan. The aim of this study was to compare the genomic sequence of clinical virulent P. gingivalis strains PGC1 and TDC60 with type strains W83 and ATCC 33277. Material and Methods: In this study, P. gingivalis strain PGC1 was isolated from subgingival plaque of a patient with severe chronic periodontitis and identified with 16s rDNA sequencing. The virulent properties of the PGC1 was analyzed with a mouse subcutaneous soft tissue abscess model. Draft genome of P. gingivalis PGC1 was sequenced by high-throughput Solexa sequence analyzer. Finally, the protein-coding sequences (CDSs) of PGC1 was compared with those of TDC60 , W83, and ATCC 33277. Results: In the animal model, strain PGC1 showed typical virulent properties, which are comparable with strain W83. In the genomic analysis, 2,111 CDSs were predicted. Further genomic comparison revealed the number and function categories of genes unique, shared, or core between the strains PGC1, TDC60 , W83, and ATCC 33277. Conclusion: This study provides new insight in virulence study of P. gingivalis.

Topic: Aetiology and pathogenesis

P0004

Results: The etiology of peri-implantitis cases was determined to be ineffective cement or calculus removal in 36% of patients. Open contacts and incomplete seating of the crown resulted in osteitis around the implant in 54% of cases. Moreover, a reported allergy to penicillin was associated with 56% of overt implant failures.

Gingival crevicular fluid levels of interleukin-33 (IL-33), thymic stromal lymphopoietin (TSLP), interferon-γ (IFN-γ), and interleukin-4 (IL-4) in periodontal health and disease.

Conclusion: Two factors under the control of the clinician ineffective implant maintenance and the incomplete removal of cement at the time of crown cementation - are positively associated with peri-implantitis. A correlation of a penicillin allergy with implant failure may suggest a link with the innate osteoimmunity of the patient.

E. Papathanasiou1, F. Teles2, T. Theoharides1, E. Arguello1, T. Griffin1, J. Hanley1

Topic: Aetiology and pathogenesis

P0002 Genome comparison of clinical virulent Porphyromonas gingivalis strains PGC1 and TDC60 with type strains W83 and ATCC 33277 D. Liu, R. Shu Shanghai/China Aim: Porphyromonas gingivalis is a major pathogen of chronic periodontitis. In mouse abscess model, P. gingivalis strains show virulent and less-virulent properties. Two widely studied type strains of P. gingivalis, virulent strain W83 and less-virulent strain ATCC 33277, have been genome-sequenced in 2003

1Boston/United States of America, 2Cambridge/United States of America

Aim: Interleukin-33 (IL-33) and thymic stromal lymphopoietin (TSLP) are novel cytokines, highly involved in the immunoregulation of several inflammatory diseases. The goals of this study are to investigate the presence and levels of IL-33 and TSLP, as well as IFN-γ and IL-4 in gingival crevicular fluid (GCF) in periodontal health and disease. Material and Methods: Periodontally healthy (n= 14), gingivitis (n=17) and periodontitis (n=11) subjects were recruited. Clinical data performed and GCF samples were collected from healthy (H), gingivitis (G) and periodontitis (P) sites in each subject category (i.e., PH, PG, PP in periodontitis subjects; GH, GG in gingivitis subjects and HH in healthy subjects). IFN-γ, IL-4, IL-33, and TSLP levels were evaluated using a multiplex bead immunoassay (Luminex). Differences between and within groups were sought using the Mann Whitney and Wilcoxon tests, respectively. Results: Inflamed sites in gingivitis and periodontitis patients showed higher GCF volume than non-inflamed sites in all patient categories (p0.05). For both regimens a significant decrease was obtained for the Oral Chroma™ H2S and CH3SH readings after the intervention. For CH3SH both regimens resulted in approximately a 60% reduction, where as for H2S, the reduction was between 30-50%. Overall, no difference was noted between the drinking or rinsing group for any of the parameters. Conclusion: Drinking a glass of water or rinsing with 15ml water had a statistically significant effect on the MBB outcome. There was no significant difference between the regimens. The

© 2012 European Federation of Periodontology

P0345 IMMUNE MODULATORY AND ANTIOXIDANT EFFECTS OF MELATONIN IN PERIODONTITIS INDUCED RATS C.F. Canakci, S. Akman, S. Yildirim, H.H. Alp, A. Kara Erzurum/Turkey Aim: Melatonin is a hormone (N-acetyl-5 methoxytryptamine) synthesis from pineal gland especially in night. Periodontal disease causes of the oxidative stress to periodontal tissues. We aim to investigate the immune modulatory and antioxidant role of melatonin in treatment of periodontitis. Material and Methods: The rat’s mandibular first molar teeth were ligatured in submarginal position for 4 weeks to induce periodontitis and then 4 weeks after the ligature was removed. 24 animals were randomly divided into 3 groups (Control, PED, MEL+PED). Therefore, the melatonin administration were applied to only MEL+PED group at a i.p. dose of 10 mg/kg of body weight for 2 weeks in the night. Results: The results of present study suggest that administration of melatonin prevent oxidatively modified DNA and oxidative damages in periodontal tissues. In histologic examination, melatonin administration group collagen density and epithelium structure nearly were similar to control group tissues. Conclusion: According to our data, melatonin has reduced the oxidative damage in the periodontitis-induced rats periodontal tissue via inhibition of inflammatory effects and activate ascorbic acid-mediated glutathione.

Topic: Clinical Research: Periodontal Therapy

P0346 IMMUNE MODULATORY AND ANTIOXIDANT EFFECTS OF MELATONIN IN PERIODONTITIS INDUCED RATS S. Akman, C.F. Canakci, S. Yildirim, H.H. Alp, A. Kara Erzurum/Turkey Aim: Melatonin is a hormone (N-acetyl-5 methoxytryptamine) synthesis from pineal gland especially in night. Periodontal disease causes of the oxidative stress to periodontal tissues. We aim to investigate the immune modulatory and antioxidant role of melatonin in treatment of periodontitis. Material and Methods: The rat’s mandibular first molar teeth were ligatured in submarginal position for 4 weeks to induce periodontitis and then 4 weeks after the ligature was removed. 24 animals were randomly divided into 3 groups (Control, PED, MEL+PED). Therefore, the melatonin administration were applied to only MEL+PED group at a i.p. dose of 10 mg/kg of body weight for 2 weeks in the night. Results: The results of present study suggest that administration of melatonin prevent oxidatively modified DNA and oxidative

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damages in periodontal tissues. In histologic examination, melatonin administration group collagen density and epithelium structure nearly were similar to control group tissues Conclusion: According to our data, melatonin has reduced the oxidative damage in the periodontitis-induced rats periodontal tissue via inhibition of inflammatory effects and activate-ascorbic acid mediated glutathione.

Topic: Clinical Research: Periodontal Therapy

P0347 Retrospective evaluation of non-surgical periodontal therapy in undergraduate education in Switzerland – an extended case series S. Buset, N.U. Zitzmann, L. Thillainathan, R. Weiger, C. Walter Basel/Switzerland Aim: Periodontal treatment conducted by dental students may be a suitable approach for patients with lower socioeconomic status. Objective was to analyse the efficacy of non-surgical treatment of moderate to advanced periodontal diseases in undergraduate education. Material and Methods: In a retrospective analysis, the records of patients treated by undergraduate dental students at the University of Basel, Switzerland, were consecutively screened during February 2010 and October 2011. Patients ≤ 40 years of age, treated for moderate to advanced periodontal diseases with complete documentation and at least one reevaluation appointment after non-surgical periodontal treatment were included. Periodontal, medical and socioeconomic variables were analysed. Results: The sample consisted of 19 participants, with 10 women (average age 34.2±5.2 yrs) and 9 men (34.2±5.2 yrs). Eight patients received an adjunctive antibiotic medication after mechanical instrumentation of the diseased sites. The number of multirooted teeth did not differ significantly between baseline and the second reevaluation (p=0.474). The mean number of sites with probing pocket depth (PPD) 7 mm). Results: The sample included 130 patients (41% males; 59% females). Ethnicities were 40% African, 34.6% East Indian and 25.4% of mixed descent. The mean site gingival recession was 1.56 ± 1.42. Mean gingival recessions for all six sites were significantly greater among Indo-Trinidadians versus AfroTrinidadians (p 0.05). Conclusion: The results of this study provide evidence that periodontitis is associated with increased systolic blood pressure among Brazilian Indians.

Vienna/Austria Aim: All patients at the Bernhard Gottlieb University Dental Clinic are screened for periodontal disease at first visit. The PGU (parodontale Grunduntersuchung) is used. Patients with PGU indices >3 are referred for periodontal treatment and full mouth measurements are taken: The aim was 1. to assess the prevalence of periodontal disease in patients seeking dental treatment and 2. to assess the correlation of PGU scores with the deepest probing pocket depth in the respective sextant in two WHO cohorts (35-44 and 65-74 yrs of age). Material and Methods: Data from records of all patients 18-74yrs, seeking treatment at the BGZMK during 2008 and 2010 were extracted: chief complaint, age, gender, smoking status, number of teeth; and descriptive statistics performed. In the two cohorts Cohens Kappa Coefficient was used to describe the correlation of PGU and pocket depth in the respective sextants for males/females, smokers/nonsmokers. Results: 5.350 patients were eligible: 2.899 females and 2.451males. Reported smoking status was 41 vs. 53%. Chief complaint was pain or periodontal problems (27 resp. 17%). In the younger cohort were 131, in the older 52 patients. Correlation between PGU scores and probing pocket depths values showed little to moderate correlation. Correlation was higher in frontal sextants, but only in the younger cohort. PGU score 2 and 3 values underestimated the parodontal situation, PGU score 4 values showed the highest correlation, but tended to overestimate the parodontal treatment necessity. Conclusion: Prevalence of periodontal disease in this population is comparable with other European countries. PGU underestimates periodontal treatment necessity more than it overestimates it.

Topic: Host microbial interactions

P0700 Topical application of sonicated and heat-treated Staphylococcus aureus or Aggregatibacter actinomycetemcomitans into rat gingival sulcus induces periodontal tissue destruction after immunization F. Nagano, Y. Yoshinaga, T. Kaneko, A. Kuramoto, S. Nakatsu, Y. Takamori, T. Kishimoto, S. Noguchi, Y. Hara Nagasaki/Japan Aim: Periodontitis is generally accepted that bacteria and host defense system influence its onset and progression. However,little is concretely known about their interaction on periodontal destruction. Recently we reported that immune complexs of LPS and its specific antigen forming in gingival sulcus induce periodontal destruction(Kuramoto, J Periodontal Research, in press).In this study,we used sonicated and heat-treated Staphyrococcus aureus(S. a.)or Aggregatibactor actinomycetemcomitans(A. a.) after immunization and then histopathologically examined their influences on the periodontal tissue. Material and Methods: Rats previously immunized with S. a. or A. a. were used as the immunized groups. The non-immune group received sterile PBS. Each sample was applied topically into the palatal gingival sulcus of first molars in both groups every 24 hours for 10 days. The first molar regions were resected and observed histolopathogically. Blood samples were collected from the rats on 0 day, 5 days and 10 days in the experimental period and the serum level of anti-S. a. or A. a. antibodies were determined by ELISA. Results: Significant attachment loss, increasing apical migration of


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junctional epithelium and inflammatory reactions such as neutrophils’s infiltration were observed in the immunized groups. The serum levels of anti-S. a. and -A. a. antibodies in the non-immunized groups showed no significant change. Conclusion: S. a. and A. a. could induce attachment loss in immunized rat. The results suggest that not only Gram-negative but also Gram-positive bacteria involve in periodontal destruction under the existence of high serum level of their specific antibody.


P0701 Clinical and histological characteristics in Bisphosphonate- Related Osteonecrosis of the Jaws associated with Actinomyces: Comparison between patients on PO and IV Bisphosphonates K. Anavi Lev1, G. Chaushu2, Y. Anavi2, I. Kaplan2 1Jerusalem/Israel, 2Petah-Tiqva/Israel

Aim: To compare clinical and histological findings of patients on PO versus IV Bisphosphonates with Bisphosphonate- Related Osteonecrosis of the Jaws (BRONJ) associated with Actinomyces, and to correlate the clinical course and treatment requirements with the finding of histomorphometric analysis. Material and Methods: The study was a 10-year retrospective analysis of archived cases with BRONJ. Actinomyces colonies were identified using hematoxylin-eosin, Gram, and periodic acid–Schiff stains, exhibiting filamentous morphology with color variation between center and periphery. Only colonies with adjacent tissue reaction (inflammation, fibrosis) were analyzed. Actinomyces density (AD) was calculated by dividing the total number of colonies by tissue surface, Actinomyces relative surface (ARS) was calculated by dividing total bacterial colony surface by tissue surface. Results: The study includes 53 patients (39 on IV and 14 on PO Bisphosphonates). The average AD was 4.25 ± 6.72 in patients on IV Bisphosphonates and 7.25 ± 11.81 in patients on PO Bisphosphonates, with no significant differences between groups. The average ARS was 0.06 ± 0.10 in patients on IV Bisphosphonates and 0.11 ± 0.13 on patients on PO Bisphosphonates, with no significant differences between groups. No significant differences were found in the duration of antibiotic treatment nor the duration of healing between groups. Conclusion: Although Actinomyces was abundant in BRONJ in both IV and PO groups, there were no significant differences in duration of antibiotic treatment or the duration of healing, that could indicate that Actinomyces may have a detectable impact on disease duration or outcome.


P0702 Lactobacillus reuteri modulates the secretion of pro-inflammatory cytokines by human macrophage-like cell line U-937 F. Brito1, C.M. Figueredo1, A. Gustafsson2, A.C.D.P. Rosa1, A.L.D.M. Guaraldi1, R.H. Junior1 1Rio De Janeiro/Brazil, 2Stockholm/Sweden

Poster abstracts

Aim: To assess the cytokine response patterns observed following stimulation of the L. reuteri with human macrophage-like cell line U-937. Material and Methods: L. reuteri Prodentis obtained from a commercial lozenge: Periobalance (BioGaia AB, Sweden) and a potentially pathogenic bacterium Escherichia coli (E. coli) (strain EAEC I49) were used to stimulate the human macrophage-like cell line U-937. The experiment was performed in two steps. In the first step, each bacterium was used to stimulate U-937 cells separately. In the second step, U-937 cells were first stimulated with the probiotic L. reuteri and then stimulated with E. coli. Non stimulated U-937 cells represented the negative controls. Cytokine concentrations of IL-6, IL-8, IL-10, IFNg and TNF-α were measured from culture supernatants using a commercially available Lincoplex kit. Results: E. coli induced the production of higher quantities of cytokines, whereas both negative control and L. reuteri induced similar productions of IL-6, IL-8, INFg and TNF-α by human macrophage-like U937. Pre-incubation of U937 cells with L. reuteri and subsequent incubation with E. coli, induced lower concentrations of cytokines, when compared to negative control. Conclusion: Lactobacillus reuteri is a probiotic that downregulates the production of pro-inflammatory cytokines by human macrophage-like cell line U-937.


P0703 Heat shock protein 27 expression in oral lichen planus lesions from different oral epithelium and control mucosa V. García-García1, A. Bascones-Martínez1, A.I.G. Kass1, C.P. Martinelli-Kläy2, R. Küffer2, T. Lombardi2 1Madrid/Spain, 2Geneve/Switzerland

Aim: Oral lichen planus (OLP) has been considered a chronic disease .There have been described precipitating factors such as dental plaque. There exists the possibility of a role for the Heat-shock proteins (HSPs) in the beginning or persistence of the lymphocytic response that takes place in the pathogenesis of this entity. The aim of this study is to highlight the not very known dynamic evolution of OLP and to analyze the expression of hsp27 in normal and lichen planus-affected oral mucosae and to compare the expression in the diverse stages of OLP lesions in respect to the healthy mucosa in order to evaluate the biological and/or prognostic role of hsp27 in OLP pathogenesis. The possible interactions between HSPs and periodontal disease described in the literature will be also presented. Material and Methods: 36 cases of OLP and 10 cases of health patients were selected. hsp 27 expression was analysed by means of immunohistochemistry. Influence of sex, age and anatomical location was also studied. Results: Hsp 27 expression varied between the different groups and there were statistically significantly differences. Conclusion: Altogether, the present data suggest that hsp27 may have any role in the pathway of OLP pathogenesis. In relation with the different stages of OLP, our study demonstrates that there seems to be differences between the three OLP groups.


Poster abstracts


P0704 Clinical, microbiological and immunological determinants in patients with different forms of periodontitis Y.G. Chumakova Odessa/Ukraine Aim: The aim of the present study was to identify clinical, radiographic, bacterial and immunological characteristics of chronic periodontitis (CP) and aggressive periodontitis (AP). Material and Methods: Clinical and radiographic examination of 272 patients with severe CP (age 18-58 years) and 53 patients with AP (22 – juvenile periodontitis and 31 – rapidly progressive periodontitis of adults) (age 14-35 years) was conducted. Pocket probing depth and relative attachment level were measured with Florida probe and gingival bleeding index. Microbiological identification of periodontopathogens was carried out using established anaerobic culture techniques. Immunological examination included the study of cytokines regulation in blood and gingival fluid (IL-1β, IL-4, IL-6, IL-10, TNFα levels) and immunological analysis in blood (CD2, CD3, CD4, CD8, CD4/CD8, CD19, CD22, CD57 lymphocytes, IgA, IgG, LKT, C3-complement component), saliva and gingival fluid (SIgA, IgA, IgG, lysozyme, C3-complement component). Results: Clinical-laboratory features of CP and AP and main pathogenic factors were determined. The role of local factors of an oral cavity, microflora a periodontal pocket, infringements in system of local immunity of an oral cavity and periodontal tissue, cytokine disbalance are investigated. Diagnostic and treatment planning protocol of different forms of periodontitis was developed. Efficiency of various methods of antimicrobial treatment, including systemic antibiotic therapy, was evaluated. Conclusion: Long-term results of complex treatment (up to 10 years) of patients with chronic and aggressive periodontitis using non-surgical therapy, periodontal surgery, orthodontic and prosthetic methods will be shown.


P0705 Haplotype analysis of the IL-10 promoter polymorphisms and microbial composition in patients with chronic and aggressive periodontitis L. Izakovicova Holla, P. Linhartová, D. Buckova, J. Vokurka, A. Fassmann Brno/Czech Republic Aim: Interleukin-10 (IL-10) is a key regulatory cytokine that has a significant effect on both the innate and adaptive immune response. The aim of this study was to analyze polymorphisms in the IL-10 gene and their associations with microbial composition and clinical manifestation of chronic (CP) or aggressive (AgP) periodontitis. Material and Methods: Three polymorphisms [-1082G/A (rs1800896), -819C/T (rs1800871) and -592C/A (rs1800872)] in


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the IL-10 gene were studied in 256 patients with CP, 67 patients with AgP and 267 unrelated controls. All polymorphisms were detected using the PCR-RFLP methods. Subgingival bacterial colonization (occurrence of A. actinomycetemcomitans, P. gingivalis, P. intermedia, T. forsythia, T. denticola, P. micros, F. nucleatum in subgingival pockets) was investigated by the DNA-microarray based detection kit. Results: Despite significant differences determined in the occurrence of periodontal bacteria between patients with CP/ AgP and healthy controls (from p