3rd International Congress on Applied Ichthyology & Aquatic Environment
8-11 November 2018 Volos, Greece www.hydromedit.gr
PRELIMINARY RESULTS RESULTS ON ON POLYMORHISM POLYMORHISM STUDY PRELIMINARY STUDY OF OF FARMED FARMED SNAILS SNAILS Cornu aspersum maximum BASED ON MORPHOLOLOGICAL MARKERS Kougiagka E.*, Gkafas G., Exadactylos A., Hatziioannou M. Department of Ichthyology and Aquatic Environment, School of Agricultural Sciences, University of Thessaly, Fytoko Street, 38446, Νea Ionia Magnesia, Greece Abstract The aim of the present study is to evaluate authenticity of Cornu aspersum maximum snails derived from farms in Greece based on their morphological characters. Shell morphometric traits, weight, shell pattern and mantle color, were assessed in 180 snails collected in October 2017 from 9 farms in Greece. Polymorphism study based on morphological markers should be followed by genetic analysis in order to have accurate results. Keywords: Cornu aspersum maximum, polymorphism, Heliciculture *Corresponding author: Kougiagka Efkarpia (
[email protected] ) 1. Introduction Snail food products are very famous and are consumed throughout the world. Food authenticity is affected by genetic background and geographical origin (Sobolev et al. 2017). The comparison of anatomical features of organisms has been a central element in biology for centuries. Polymorphism is a basic characteristic of shell morphology, which preserves the onto-genetic record of growth, is now the main subject of a sub-field of morphometrics (Madec et al. 2003). Many phylogenetic studies have been carried out for the species of Cornu aspersum but the origin of the commercial subspecies Cornu aspersum maximum is unknown apart from few historical references (Guiller et al 2006). The aim of the present study is to evaluate authenticity of 180 C. a. maximum snails collected in 9 farms based on their morphological markers. 2. Material and Methods According to the sampling procedure, a total of 180 Cornu aspersum maximum snails from 9 farms were collected in October 2017 (Table 1). The morphological characteristics such as shell diameter (D), shell height (H), aperture diameter (d) and animal weight (W) were assessed for all snails. The collected snails were categorized according to their shell color and the existence of bands in three shell patterns (p1: light color shell with bands, p2: light color shell without bands and p3: black color shell). Finally, the mantle color was assessed as dark or light. Morphological characteristics were tested for significance at the 5% level by using one way analysis of variance (ANOVA). Post-hoc comparisons were performed using the Bonferroni test. Table 1. Region of farms used for snail collection. Code Region Elevation (m) F1 Central Macedonia 43 F2 Central Macedonia 109 F3 Western Macedonia 670 F4 F5 F6 F7 F8 F9
Τhrace Central Μacedonia Central Macedonia Western Greece Thessaly North Aegean
14 122 56 0 153 43
3. Results Figure 1 shows the mean values of the morphometric features and weight. Snails from F7 presented the highest values of all the markers and snails from F6 the lowest values, except for aperture diameter. No significant differences were recorded in traits D, H and W, except for d (F=11.506, P=0.0169). As it concerns aperture diameter, F6, F7 and F9 presented significant differences compared to other farms.
E-POSTERS | 5th Field-Inland Aquatic Ecology and Resources
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HydromediT 2018 | Book of Proceedings
3rd International Congress on Applied Ichthyology & Aquatic Environment
8-11 November 2018 Volos, Greece www.hydromedit.gr
Figure 1. Mean values ± SD of shell diameter (D), shell height (H), aperture diameter (d) and animal weight (W). Small numbers denote statistical significant difference: * p
