Product Insert - Cayman Chemical

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13184), resulting in antithrombotic, antiallergic, and other salutary effects.1-3 When ... mediated activation of NF-κB in RAW 264.6 macrophages when given at  ...

Product Information Puerarin

Item No. 14175 OH

CAS Registry No.: 3681-99-0 Formal Name: 8-β-D-glucopyranosyl-7-hydroxy-3-(4hydroxyphenyl)-4H-1-benzopyran-4-one Synonyms: Kakonein, NPI 031G MF: C21H20O9 FW: 416.4 Purity: ≥98% Stability: ≥2 years at -20°C Supplied as: A crystalline solid UV/Vis.: λmax: 250, 305 nm

OH

HO O

HO

OH O

O OH

Laboratory Procedures For long term storage, we suggest that puerarin be stored as supplied at -20°C. It should be stable for at least two years. Puerarin is supplied as a crystalline solid. A stock solution may be made by dissolving the puerarin in the solvent of choice. Puerarin is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide, which should be purged with an inert gas. The solubility of puerarin in these solvents is approximately 5, 12.5, and 16 mg/ml, respectively. Further dilutions of the stock solution into aqueous buffers or isotonic saline should be made prior to performing biological experiments. Ensure that the residual amount of organic solvent is insignificant, since organic solvents may have physiological effects at low concentrations. Organic solvent-free aqueous solutions of puerarin can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of puerarin in PBS, pH 7.2, is approximately 0.25 mg/ml. We do not recommend storing the aqueous solution for more than one day. Puerarin is a natural isoflavone isolated from plants of the genus Pueraria used in traditional Chinese herbal medicine. It is biotransformed by intestinal bacteria to give the phytoestrogens daidzein (Item No. 10005166) and equol (Item No. 13184), resulting in antithrombotic, antiallergic, and other salutary effects.1-3 When given intraperitoneally, puerarin evokes diverse responses by modulating serotonin receptors.4,5 This compound also suppresses lipopolysaccharidemediated activation of NF-κB in RAW 264.6 macrophages when given at 20-40 μM.6 References 1. Choo, M.-K., Park, E.-K., Yoon, H.-K., et al. Antithrombotic and antiallergic activities of daidzein, a metabolite of puerarin and daidzin produced by human intestinal microflora. Biol. Pharm. Bull. 25(10), 1328-1332 (2002). 2. Jin, J.-S., Nishihata, T., Kakiuchi, N., et al. Biotransformation of C-glucosylisoflavone puerarin to estrogenic (3S)-equol in co-culture of two human intestinal bacteria. Biol. Pharm. Bull. 31(8), 1621-1625 (2008). 3. Nakamura, K., Nishihata, T., Jin, J.-S., et al. The C-glucosyl bond of puerarin was cleaved hydrolytically by a human intestinal bacterium strain PUE to yield its aglycone daidzein and an intact glucose. Chem. Pharm. Bull. 59(1), 23-27 (2011). 4. Overstreet, D.H., Kralic, J.E., Morrow, A.L., et al. NPI-031G (puerarin) reduces anxiogenic effects of alcohol withdrawal or benzodiazepine inverse or 5-HT2C agonists. Pharmacol. Biochem. Behav. 75(3), 619-625 (2003). 5. Chueh, F.-S., Chang, C.-P., Chio, C.-C., et al. Puerarin acts through brain serotonergic mechanisms to induce thermal effects. J. Pharmacol. Sci. 96, 420-427 (2004). 6. Hu, W., Yang, X., Zhe, C., et al. Puerarin inhibits iNOS, COX-2 and CRP expression via suppression of NF-κB activation in LPS-induced RAW264.7 macrophage cells. Pharmacol. Rep. 63, 781-789 (2011).

Cayman Chemical Related Products For a list of related products please visit: www.caymanchem.com/catalog/14175

WARNING: This product is for laboratory research only: not for administration to humans. Not for human or veterinary diagnostic or therapeutic use. MATERIAL SAFETY DATA This material should be considered hazardous until information to the contrary becomes available. Do not ingest, swallow, or inhale. Do not get in eyes, on skin, or on clothing. Wash thoroughly after handling. This information contains some, but not all, of the information required for the safe and proper use of this material. Before use, the user must review the complete Material Safety Data Sheet, which has been sent via email to your institution. WARRANTY AND LIMITATION OF REMEDY Cayman Chemical Company makes no warranty or guarantee of any kind, whether written or oral, expressed or implied, including without limitation, any warranty of fitness for a particular purpose, suitability and merchantability, which extends beyond the description of the chemicals hereof. Cayman warrants only to the original customer that the material will meet our specifications at the time of delivery. Cayman will carry out its delivery obligations with due care and skill. Thus, in no event will Cayman have any obligation or liability, whether in tort (including negligence) or in contract, for any direct, indirect, incidental or consequential damages, even if Cayman is informed about their possible existence. This limitation of liability does not apply in the case of intentional acts or negligence of Cayman, its directors or its employees. Buyer’s exclusive remedy and Cayman’s sole liability hereunder shall be limited to a refund of the purchase price, or at Cayman’s option, the replacement, at no cost to Buyer, of all material that does not meet our specifications. Said refund or replacement is conditioned on Buyer giving written notice to Cayman within thirty (30) days after arrival of the material at its destination. Failure of Buyer to give said notice within thirty (30) days shall constitute a waiver by Buyer of all claims hereunder with respect to said material. For further details, please refer to our Warranty and Limitation of Remedy located on our website and in our catalog. Copyright Cayman Chemical Company, 03/29/2013

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