and HAROLD. PAPKOFF2. Hormone .... Ryan,. 1983;. Sairam,. 1983). Equine. LH. (eLH), however, is an exception to the rule. It has been ... 10 M urea as previously described. (Papkoff,. 1974). The. oLH subunits were prepared by the.
BIOLOGY
OF
REPRODUCTION
35,
Properties
493-500
(1986)
of Equine
Luteinizing
and in Combination JANET
F. ROSER,3
Hormone
with
FRANK
Alpha
Various
Alone
Beta Subunits’
CARRICK,4
N.
Subunit
and
HAROLD
PAPKOFF2
Hormone Research Laboratory University of California San
Francisco,
California
94143
ABSTRACT Previous
studies
have
monophosphate from
immature
rats.
subunit.
To
bination
with
In
shown
(cAMP)
It was
explore
this
ovine
a radioreceptor
that
induced
equine
by
also
shown
that
phenomenon
(o)
LI-I
assay
beta,
luteinizing
hormone
follicle-stimulating the
inhibitory
further, ovine
employing
the
FSH
inhibits
(FSH)
in preparations
effect
intrinsic
beta,
‘251-oFSH
(eLH)
hormone
and and
a function
FSH-like
equine testis
was
FSH
production of the
activities beta
were
membranes
from
of cyclic
of
adenosine
of seminiferous equine
eLH
alpha
evaluated
calves,
(eLH)
alone
in several
day-old
tubules
LH
and assay
eLH
alpha in comsystems.
was
twice
as
active as oFSH, eLH alpha was 6% as active as oFSH, and other subunits Whereas oLH was only 0.1% as active as oFSH, the hybrid eLH alpha-oLI-I activity of eLH alpba-FSH beta hybrids tended to be higher than the