Supplementary data (IJSEM paper no. IJS/2012/047712)
Pseudomonas guguanensis sp. nov., a Gammaproteobacteria isolated from Guguan hot spring in Taiwan You-Cheng Liu1, Li-Sen Young2, Shih-Yao Lin1, Asif Hameed1, Yi-Han Hsu1, Wei-An Lai1,3, Fo-Ting Shen1,3, Chiu-Chung Young1,3
1
Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taichung, Taiwan
2
Department of Biotechnology, College of Applied Arts and Sciences, National Formosa University, Yunlin, Taiwan
3
Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan
Running title: Pseudomonas guguanensis sp. nov. Category: New taxa Proteobacteria
Corresponding authors: Professor Dr. Chiu-Chung Young National Chair Professor, Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, 250, Kuo Kuang Rd., Taichung, 402, Taiwan Tel./fax: +886422861495 E-mail address:
[email protected]
87 58 59
IAM 1529 T (AB039395)
Pseudomonas cremoricolorata BCRC 17510T (GQ996724) Pseudomonas parafulva BCRC 17511T (FJ418638) Pseudomonas putida
99
ATCC 12633 T (AB039451)
Pseudomonas plecoglossicida CIP 106493 T (FN554218) Pseudomonas mosselii BCRC 17518 T (FJ418640)
60
51
Pseudomonas fulva
69
Pseudomonas monteilii
BCRC 17520 T (FJ418641)
Pseudomonas agarici Pseudomonas jessenii
93
72 100
NCPPB 2289 T (AB039457) DSM 17150 T (AM293562)
Pseudomonas asplenii NCPPB 1947 T (AB039455) Pseudomonas fuscovaginae BCRC 17126 T (GQ996723)
Pseudomonas flavescens
LMG 18387 T (FN554183)
Pseudomonas borbori LMG 23199T (FN554175) Pseudomonas argentinensis LMG 22563 T (FN554170) Pseudomonas chloritidismutans AW-1T (AJ880092) Pseudomonas stutzeri IFO 14165 T (AB039393)
94 95
Pseudomonas xanthomarina
CCUG 46543 T (AM905836)
Pseudomonas oryzihabitans BCRC 16007T (FJ418636) Pseudomonas sagittaria CC-OPY-1T (JQ897956) Pseudomonas alcaligenes
BCRC 11893 T (AB039388)
Pseudomonas tuomuerensis 74 57
JCM 14085T (AB571150)
Pseudomonas guguanensis CC-G9AT (JQ864238) Pseudomonas toyotomiensis JCM 15604 T (AB494447) Pseudomonas alcaliphila DSM 17744 T (AB494446) Pseudomonas mendocina
79
99
LMG 1223 T (AJ633103)
Pseudomonas oleovorans subsp lubricantis RS1 T (EF675630) Pseudomonas indoloxydans JCM 14246T (AB548144) P. oleovorans subsp. oleovorans ATCC 8062 T (AB039396) Pseudomonas formosensis CC-CY503T (JQ864235)
0.02
Figure S1. Phylogenetic analysis of strain CC-G9AT and other Pseudomonas spp. based on amino acid encoded from gyrB gene. Distances and clustering were performed by using neighbor-joining method with the software package MEGA version 5. Numbers (>50%) at branch points are bootstrap percentages based on 1,000 replicates. Bars, 0.01 changes per nucleotide position.
54
64
Pseudomonas fulva CIP 106765 T (AJ717419) Pseudomonas parafulva CIP 107617 T (AJ717471) Pseudomonas monteilii CFML 90-58 (AJ786273) Pseudomonas cremoricolorata CIP 107616 T (AJ717476)
Pseudomonas mosselii ATCC BAA-99 T (FN554744) Pseudomonas putida LMG 2257 T (AJ717474) 89 Pseudomonas plecoglossicida CIP 106493 T (AJ717456) Pseudomonas taiwanensis BCRC 17751T (HE577797) Pseudomonas japonica JCM 21532 T (HE577800) Pseudomonas vranovensis DSM 16006 T (HE577799) ICMP 5940 T (FJ483529) 98 Pseudomonas fuscovaginae Pseudomonas asplenii LMG 2137 T (AJ717432) Pseudomonas moraviensis DSM 16007 T (FN554743) Pseudomonas agarici LMG 2112 T (AJ717477)
53
Pseudomonas reinekei CCUG 53116 T (FN554754) Pseudomonas jessenii CIP 105274 T (AJ717447) 98 89 Pseudomonas vancouverensis CIP 106707 T (AJ717473) T Pseudomonas argentinensis LMG 22563 (FN554728) 74
50 67
89
Pseudomonas flavescens CIP 104204 T (AJ717468) Pseudomonas straminea LMG 21615 T (FN554758) Pseudomonas alcaligenes BCRC 11893 T (AJ717475) Pseudomonas alcaliphila DSM 17744 T (AJ717463) Pseudomonas toyotomiensis JCM 15604 T (AB548147) Pseudomonas mendocina
63
87
99
55
82
BCRC 10458 T (KF029437)
Pseudomonas guguanensis CC-G9AT (JQ864239) Pseudomonas oleovorans subsp. oleovorans ATCC 8062 T (AJ717461) Pseudomonas borbori LMG 23199 T (FN554730) Pseudomonas oryzihabitans CIP 102996 T (AJ717470)
Pseudomonas sagittaria CC-OPY-1 T (JQ277454) Pseudomonas otitidis DSM 17224 T (FN554745) Pseudomonas tuomuerensis JCM 14085T (AB571151) Pseudomonas stutzeri CIP 11199 T (AJ717459) Pseudomonas xanthomarina CCUG 46543T (FN554765) Pseudomonas xiamenensis C10-2 T (EF667149) 99 Pseudomonas pertucinogena LMG 1874T (AJ717441) Pseudomonas formosensis CC-CY503 T (JQ864236) Pseudomonas pachastrellae CCUG 46540 T (FN554746) 95
55
Pseudomonas sabulinigri CECT 7679 T (FN908491) Pseudomonas pelagia CECT 7689 T (FN908494)
0.01
Figure S2. Phylogenetic analysis of strain CC-G9AT and other Pseudomonas spp. based on amino acid encoded from rpoB gene. Distances and clustering were performed by using neighbor-joining method with the software package MEGA version 5. Numbers (>50%) at branch points are bootstrap percentages based on 1,000 replicates. Bars, 0.05 changes per nucleotide position.
Figure S3. Cell morphology of strain CC-G9AT was observed by transmission electron micrographs. Scale bar 1 μm.
