hydroxylase, reductase and protein B, which is involved in enzyme .... incubated for 15 min at 30°C. The - or -naphthol formed was measured ..... Green et al.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 1999, p. 3929–3935 0099-2240/99/$04.00⫹0 Copyright © 1999, American Society for Microbiology. All Rights Reserved.
Vol. 65, No. 9
Purification and Characterization of the Soluble Methane Monooxygenase of the Type II Methanotrophic Bacterium Methylocystis sp. Strain WI 14 STEPHAN GROSSE,1 LOUISE LARAMEE,2 KARIN-DAGMAR WENDLANDT,3 IAN R. MCDONALD,4 CARLOS B. MIGUEZ,2 AND HANS-PETER KLEBER1* Institut fu ¨r Biochemie, Fakulta ¨t fu ¨r Biowissenschaften, Pharmazie und Psychologie, Universita ¨t Leipzig, D-04103 Leipzig,1 and Sektion Sanierungsforschung, Umweltforschungszentrum Leipzig-Halle GmbH, D-04318 Leipzig,3 Germany; Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec, Canada H4P 2R22; and Department of Biological Sciences, University of Warwick, Coventry, United Kingdom4 Received 24 February 1999/Accepted 17 June 1999
Methane monooxygenase (MMO) catalyzes the oxidation of methane to methanol as the first step of methane degradation. A soluble NAD(P)H-dependent methane monooxygenase (sMMO) from the type II methanotrophic bacterium WI 14 was purified to homogeneity. Sequencing of the 16S rDNA and comparison with that of other known methanotrophic bacteria confirmed that strain WI 14 is very close to the genus Methylocystis. The sMMO is expressed only during growth under copper limitation (
