Purification, cloning and expression of spinach leaf

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of spinach leaf sucrose-phosphate synthase in Escherichia coli. U. Sonnewald1 .... Extraction bulTer contained 50 mM 4-(2-hydroxyethyl)-l-pipcra-.
Planta (1993) 189: 174-181

Planta

'.)'.:: Springer-Verlag 1993

Purification, cloning and expression of spinach leaf sucrose-phosphate synthase in Escherichia coli U. Sonnewald 1 , W.P. Quick 2 *, E. MacRaeUd:, K.-P. Krause 2 **\ and Mark 1 2

Stitt 2 *~'*

lnstitut flir Genbiologische Forschung, lhnestrasse 63. W-1000 Berlin 33, FRG Lehrstuhl flir Pflanzenphysiologie, UniversiHit Bayreuth. Postfach 101251, W-8580 Bayreuth, FRG

Received 2 May: accepted 30 June 1992

Abstract. Sucrose-phosphate synthase (SPS) from leaves of spinach (Spinacia olerncea L.) has been purified to homogeneity by a procedure involving precipitation with polyethylenglycol and chromatography over diethyl(/) Q)

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