Quantitative phosphoproteomics reveals the role of SnRK1 as metabolic master regulator under energy deprivation Ella Nukarinen1,*, Thomas Nägele1,2,*, Lorenzo Pedrotti3,*, Bernhard Wurzinger1, Andrea Mair1, Ramona Landgraf4, Frederik Börnke4,5, Johannes Hanson6, Markus Teige1, Elena BaenaGonzalez7, Wolfgang Dröge-Laser3, Wolfram Weckwerth1,2 1
Department of Ecogenomics and Systems Biology, University of Vienna, Vienna, Austria
2
Vienna Metabolomics Center (VIME), University of Vienna, Vienna, Austria
3
Julius-von-Sachs-Institut, Julius-Maximilians-Universität Würzburg, Würzburg, Germany
4
Plant Health, Plant Metabolism Group, Leibniz-Institute of Vegetable and Ornamental Crops, Großbeeren, Germany 5
Institute of Biochemistry and Biology, University of Potsdam, Potsdam, Germany
6
Department of Plant Physiology, Umeå University, Umeå, Sweden
7
Instituto Gulbenkian de Ciência, Oeiras, Portugal
*
These authors contributed equally to this work
Correspondence and requests for materials should be addressed to W.W. (email:
[email protected])
Supplementary File S1 LOCUS OCT-2015 SOURCE ORGANISM COMMENT
Atraptor\1b\in\p
16714 bp
DNA
circular
13-
This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|747660692| COMMENT VNTDBDATE|747661067| COMMENT LSOWNER| COMMENT VNTNAME|Atraptor 1b in pBIN19 YFP (ML747)| COMMENT VNTAUTHORNAME|Bernhard Wurzinger| FEATURES Location/Qualifiers promoter 12119..12535 /vntifkey="29" /label=1x35\Promoter enhancer 12544..12674 /vntifkey="9" /label=TL\(TEV\Leader) misc_feature 12525..12567 /vntifkey="21" /label=möglicherweise\kein\XhoI terminator 747..950 /vntifkey="43" /label=Terminator\(CaMV) polyA_signal 916..922 /vntifkey="25" /label=Poly(A)\Signal misc_feature 7..735 /vntifkey="21" /label=YFP misc_feature 730..765 /vntifkey="21" /label=XbaI\nicht\da!!! primer_bind complement(70..88) /vntifkey="28" /label=EGFP-N1-R rep_origin complement(7127..7744) /standard_name="oriV" /vntifkey="33" /label=oriV /note="shows similarity to GenBank Accession Number M20134" misc_feature complement(6781..7052) /vntifkey="21" /label=kilA /note="shows similarity to kilA: GenBank Accession Number M62846" CDS complement(6430..6780) /vntifkey="4" /label=NPTIII /note="shows similarity to NPTIII gene: GenBank Accession Number V01547" transposon complement(5660..6429) /vntifkey="44" /label=IS1 /note="shows similarity to transposable element IS1: GenBank Accession Number X58999" misc_feature complement(3185..4666) /vntifkey="21" /label=trfA
/note="shows similarity to trfA: GenBank Accession Number X00713" misc_feature
Number L13842" CDS
Number J01636" rep_origin
X02513" CDS
complement(2142..3184) /vntifkey="21" /label=tetA /note="shows similarity to tetA: GenBank Accession complement(1424..1554) /vntifkey="4" /label=lacI /note="shows similarity to lacI: GenBank Accession complement(1123..1423) /standard_name="M13 ori" /vntifkey="33" /label=M13\ori /note="shows similarity to GenBank Accession Number complement(11837..11977) /vntifkey="4" /label=lacI /note="shows similarity to lacI: GenBank Accession
Number J01636" misc_feature
complement(11395..11836) /vntifkey="21" /label=geneIII /note="shows similarity to Accession Number V00604" repeat_region complement(10101..10262) /vntifkey="34" /label=RB /note="shows similarity to GenBank Accession Number J01826" misc_feature complement(8905..10088) /vntifkey="21" /label=tetA /note="shows similarity to Number X75761" rep_origin complement(8534..8912) /standard_name="ColE1 ori" /vntifkey="33" /label=ColE1\ori /note="shows similarity to V00268" misc_feature complement(7757..8540) /vntifkey="21" /label=traF /note="shows similarity to Number X54459" repeat_unit complement(1555..1702) /vntifkey="35" /label=LB CDS complement(4667..5659) /vntifkey="4" /label=NPTIII primer_bind complement(12038..12052) /vntifkey="28" /label=M13rev primer_bind complement(974..990) /vntifkey="28"
geneIII: GenBank
T-DNA right border:
tetA: GenBank Accession
GenBank Accession Number
traF: GenBank Accession
/label=M13fwd 1266..1287 /vntifkey="28" /label=LBa1\primer primer_bind 1465..1486 /vntifkey="28" /label=LBb1\primer CDS 10568..11116 /vntifkey="4" /label=Basta-R misc_feature 12680..16713 /vntifkey="21" /label=Atraptor1b BASE COUNT 3810 a 4583 c 4305 g 4016 t ORIGIN 1 ggccgcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag 61 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc 121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg 181 cccaccctcg tgaccacctt cggctacggc ctacagtgct tcgcccgcta ccccgaccac 241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc 301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac 361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg 421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag 481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag 541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 601 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac 661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 721 aagagcgggc gctaggctct agagtccgca aaaatcacca gtctctctct acaaatctat 781 ctctctctat tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc 841 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg 901 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctactag 961 tgagctcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 1021 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 1081 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgcccgc tcctttcgct 1141 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 1201 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg 1261 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg primer_bind
1321 gagtccacgt caaccctatc 1381 tcgggctatt atcaaacagg 1441 attttcgcct cagggccagg 1501 cggtgaaggg accccagtac 1561 attaaaaacg tacaccacaa 1621 tatatcctgc aaaatcacca 1681 ctcgatacag tgtaaggcgg 1741 cagactttgc gccgggtttg 1801 aaacacggat agcgttgctg 1861 cctgtgatca ttattcattt 1921 ctcgcttaac aggaaatcgc 1981 tggataaagc cgttgacgat 2041 atcaactccc agttccgact 2101 gtcggcctga agcccagtaa 2161 ggaaacaact aggttaaacc 2221 cgctccgatc gcatcgggat 2281 tggcggatca ccagttgcca 2341 ggcggtaaag cggttccgaa 2401 cgccatggaa gcgctgcgtt 2461 tggtgtcaac ggaccgccat 2521 caatcgtatc gcggctgcac 2581 agcgcctacc acaacaagct 2641 ccagaatagc agattcccgt 2701 tggaatctgt gcagcagcat 2761 accggcgacc gatgcgcctt 2821 gtgagcgtcc tctcgccgtc 2881 gatgtaggcg ccatgggctt 2941 tttctcctcg gggccgacaa 3001 tcggatctcg gagccttaat 3061 cacaattgtc cgtgcgtccc
tctttaatag tggactcttg ttccaaactg gaacaacact cttttgattt ataagggatt ttgccgattt cggaaccacc gctggggcaa accagcgtgg accgcttgct gcaactctct caatcagctg ttgcccgtct cactggtgaa aagaaaaacc tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt caccagccag ccaacagctc cccgaccggc agctcggcac gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tcatgttacc gatgctattc ggaagaacgg caactaagct gatctcgcgg agggtagcat gttgattgta acgatgacag aatatcatct ccctcgcaga gatccgaatt atcagccttc cgtgacaggc tgtcgatctt gagaactatg ccgacataat cgctgaggaa gctgagtggc gctatttctt tagaagtgaa ctatccattg ctcaccgaat ggtacaggtc ggggacccga tgcatccccg gctgatcgac cccagatctg gggctgagaa gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggccgagcc acgccaggcc gagaacattg gttcctgtag aacactaaag ctactggaac gagcagaagt cctccggccg gtgagcagag gcacgggagg ttgccacttg cgggtcagca accgcccccg ccaggcccgc tgcgacgccg acaggatcta accaacagcg ccacgcccgc agttccgcaa atagccccca gggctaccta gcagagcggc agagatgaac acgaccatca gtcgccgcga ccccgcccgg caggcggtag accgaaataa gaaatattaa gtgcgccgag gatgaagatg cgcatccacc cggacgatca tcacgagcaa taaacccgcc ggcaacgccc cctcggcctc gctgttcggg ctccacgaaa acgccggaca ttggggccgt cctcctgttt gaagaccgac agcccaatga ccgaatgcca cggcatctcg caaccgttca gcgaacgcct tgctcgtaaa cggacccgaa catctctgga gctttcttca cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct aattttaatc ctctgtttat cggcagttcg tagagcgcgc
3121 gagcgatact aaatgccagt 3181 aaagcgctgg gcgtttgcaa 3241 tgcaccaggt caactcttcg 3301 caggcttcgc tccgcacatg 3361 aggcggaagg atagtcgaac 3421 atccgtcggg cgtgtagtgg 3481 tcgccagcaa ggacgttttc 3541 ttgccacggt gtgcccaacg 3601 cggtcggacg ctcggccttc 3661 gtgtaatacc gaacgtgaag 3721 gtgatcggct ccacaccagt 3781 tcgtcatcgt cttgttgacg 3841 tggaaaatga cgtggtgaac 3901 agggcagagc cggcgcaata 3961 tcgaacaagg caacgcggcc 4021 tgcttggcct cttcttggtc 4081 gtcatagttc ctcctgttcg 4141 agacgacgcg agccagttgc 4201 acgctgtcgc ggactggaag 4261 gtttcgcggg ctcggcggaa 4321 aaccccgcgt attcattcac 4381 cctccttgcg ctgatttgac 4441 ccgcctggtg ggtcccgtag 4501 accgtctggc gaataccagc 4561 gaccccttgc cttgatgcgg 4621 aagaagtcgg ggtactaaaa 4681 caattcatcc tccccagtaa 4741 gtcaaaaaat accggacgca 4801 gaaggcaatg taaagccact 4861 tactttgcca aaaagacaag
gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg ctgctgaacc cccagccgga actgacccca caaggcccta catcattgac ccaggcgtgt tccaccaggc cgctgcctcg cgacctgctc gcgccacttc ttcacgcggg tggaatccga tttccagctt gagcgggtac ggctcccggt gcgagctgaa ccgtcggcga cagcttgcgg tacttctccc atatgaattt acagcacgac gatttcctcg tcgatcagga cctggcaacg ccaggacgcg gaagcggtgc agcagcgaca ccgattccag tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc ggccattgat cgaccagccc aggtcctggc aaagctcgta cgccgatagg ggtgcgcttc gcgtactcca acacctgctg cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag cgctgacctg ttttgccagg tcctcgccgg cggtttttcg ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc aacgctccac ggcggccgat ggcgcgggca gggcaggggg gctcgatctt ggccgtagct tgctggacca tcgagccgac gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc cgatcagttc ttgcctgtat gccttccggt caaacgtccg ggattgcccc gactcacgcc ggggcaatgt gcccttattc ccttggtgtc cagataatcc accttatcgg caatgaagtc cgtccttctc gtacttggta ttccgaatct tgccctgcac ccaaatactt gccgtgggcc tcggcctgag agccaaaaca tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta agtaaaatat aatattttat tttctcccaa tcaggcttga agctcgacat actgttcttc cccgatatcc tccctgatcg tcataccact tgtccgccct gccgcttctc ccaagatcaa tctttcacaa agatgttgct gtctcccagg tcgccgtggg
4921 ttcctcttcg ctttaaatgg 4981 agtgtcttct gtaagtaatc 5041 caattcggct tgtcgatgga 5101 gtgaaagagc tttgttcatc 5161 ttcatactct tgctccagcc 5221 atcatgccgt gccatagcat 5281 catgtccttt ccgtcatttt 5341 taaatatagg acattccttc 5401 cgtatctttt atattctcat 5461 tttagccatt ccaagaagct 5521 aattataaca taaataccag 5581 aaaacagctt gacggagccg 5641 attttgaaac atgatggtgt 5701 ttttgaggtg agctactgac 5761 ggggtggtgc ctctcactgc 5821 cgtaaaacat gcaccagaaa 5881 atcattgata tatgggcgtt 5941 ggcctcaaca acctcgcgca 6001 tacagccggg ggctatgtcg 6061 gggctaaatc aagacggttg 6121 ttgcgcacgt agcctgctgt 6181 caccctttga tcccgcctga 6241 agggaaagct cataacctga 6301 atctgaggca aaatcggtgg 6361 agctgcatga taagttggag 6421 tcattaccca gtttcaagca 6481 ttagtccatg agcgcgctgc 6541 ctatgccttg aagatatatt 6601 atcttatcag ttcatcctct 6661 tcgtcttggt ggtccaattc
ggcttttccg tctttaaaaa atcatacagc tcgcgcggat tcccagtttt cgcaatccac atcggccaga tcgttattca aagcggctgt ctaagctatt cgtataggga caatccgata ctgatgcact ccgcatacag ctcgataatc ttttcagggc tccgagcaaa ggacgccatc ggcctcactc atgagcagat tcaaagtgca ggacctttgg aacaggcagc tttccttcca tcccgttcca catcataggt ggtcccttta taccggctgt ttttcatttt ctcccaccag cttatatacc ttagcaggag acgcagcggt atttttcgat cagttttttc aattccggtg tattatttcc ttcctctttt ctacagtatt taaagatacc agacgaactc caattcactg ttccttgcat tctaaaacct tttcaaagtt gttttcaaag ttggcgtata acatagtatc cacaattatg ggtgatgctg ccaacttact gatttagtgt ctccagtggc ttctgtgtct atcagctgtc cctcctgttc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ggcaactgca gttcacttac accgcttctc aacccggtac tggccatgaa tggcgttgga tgccgggcaa cagcccgcat cgattttacg tcacttaaaa aactcaggcc gcagtcggta cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg gcgccagcgc tggctgtttt acgcgtatga cagtctccgg attcggtgaa cgcactatgg cgacgctggg gcgtcttatg cgtggtgata tggatgacgg atggctggcc gctgtatgaa gcacgtaatc agcaagcgat atacgcagcg aattgagcgg gcacctggca cggctgggac ggaagtcgct gtcgttctca caaagtcatc gggcattatc tgaacataaa acactatcaa attatgatag aatttacaag ctataaggtt attgtcctgg caagttttta tgctttgccc attctataga tatattgata ccccctgaaa tccttacata cggcgatatc ttctatataa tattgtcaat atattcaagg caatctgcct cctcatcctc agctttttaa atatggcgct tcatagagta attctgtaaa
6721 tcgttttcat gggtttatcg 6781 cacccccgaa aaggtaaaaa 6841 ttgccggccc ggcaggccgc 6901 cacccaggcc gccagcacct 6961 gcggcacgtc gttactgccc 7021 cgatcccggc ccgttcgcgg 7081 ccgaggggcg gagggttcga 7141 gaaggggggg gccctggtta 7201 aaaacaaggt gcggtggccg 7261 aaaaacgggc cccctcaaat 7321 gtcaataggt ggatcgcgcc 7381 cctcatctgt tatccccagg 7441 cttgtccaca gatttgcgag 7501 gctggccagc caacgccgcg 7561 ccgggtgagt gagggccaag 7621 ttttccgcga cttcgacggc 7681 gtttctggcg ggcaaccagc 7741 ccggtgagcg acttcaccag 7801 ctccgcgaag tcacgcgcac 7861 cccccggccg accacgccca 7921 tcatgacctt gcgaggatcg 7981 tggcatcacc agcaggccgc 8041 ccaggcggcc tagtccacga 8101 cgcccgtgat ctcatgccgg 8161 ccgccgccgc accttgatag 8221 gtgggctgcc tcatctgtta 8281 cgccggcggt gccaggtgcg 8341 aataagggac cctatcctgc 8401 ccggctgacg caaaatcctg 8461 tatatcgtgc ccgaagcagg
acctcggtat aatcttacct atcacctcaa atggttcgct cacgagcacg gcacccgcga ccactatgcc aagaatgccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag gccgccctca ctgcccggca cctggtcgct gaatgtcgat aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc aatggcaagg actgccagcg ctgccatttt tggggtgagg cagcccctgg ggggatggga ggcccgcgtt agcgggccgg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca ttataaatat tggtttaaaa gcaggttaaa agacaggtta ggaaaccctt gcaaatgctg gattttctgc ctgtggacag gcgcccctca tctgtcagca ctctgcccct caagtgtcaa cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt cggcccctca agtgtcaacg tccgcccctc atctgtcagt ggtatccaca acgccggcgg gcgcggtgtc tcgcacacgg cgtttgcagg gccatagacg gccgccagcc cagcggcgag tcgcaaaggc gctcggtctt gccttgctcg tcggtgatgt tcgctcttct tgatggagcg catggggacg tgcttggcaa ttttagcggc taaaaaagtc atggctctgc cctcgggcgg gccaagctcg tcctgcttct cttcgatctt cgccagcagg gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tttgtagccc tggccgacgg ccagcaggta ggccgacagg cttttcctca atcgctcttc gttcgtctgg aaggcagtac cttcctggtt ggcttggttt catcagccat ccgcttgccc agccggccag cctcgcagag caggattccc gttgagcacc agtgaagaag gaacacccgc tcgcgggtgg gcctacttca ccgttggata caccaaggaa agtctacacg aaccctttgg gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
8521 gttatgcagc gtatccggta 8581 agcggcaggg cgcctggtat 8641 ctttatagtc gtgatgctcg 8701 tcaggggggc gttcctggcc 8761 ttttgctggc tgtggataac 8821 cgtattaccg cgagcgcagc 8881 gagtcagtga cgagcgcggc 8941 cgtgaggctt ctacgggcgt 9001 ctgacgcggt gtgagtgggt 9061 tgcgctccgg acgttcctga 9121 caacgagcct tcgaacgctg 9181 cgtccggacc gagagcggag 9241 cctgttcaac tcctcaagca 9301 cggccccaac ccggccgaaa 9361 aacccgcctc ggtgcgcccg 9421 gtcgcgtgcc tgcctgaagc 9481 tgcgggcatt accgaatgcg 9541 tatgattctc ttgttcctga 9601 agtgccagta cgtgtcgtca 9661 gaccgtctac gtattcggct 9721 gcaactttgt ccaacttatc 9781 agtgataaag ggccagacgt 9841 gaaacccaac ctgtcggcat 9901 cggcctgatt cgaacgacgt 9961 caccgcccac cctgcgcacc 10021 tgtgctgggc tctcgctggc 10081 cggcgccaga gaacggataa 10141 accttttcac ctcttaggtt 10201 tacccgccaa aaacgacaat 10261 ctgatcatga acgcgggaca
ggaaaagcgc cacgcttccc gaagggagaa aggcggacag tcggaacagg agagcgcacg agggagcttc cagggggaaa ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg cttttgctca catgttcttt cctgcgttat cccctgattc cctttgagtg agctgatacc gctcgccgca gccgaacgac gcgaggaagc ggaagagcgc cagaaggccg ccagagaggc ggacgctagg gcagggcatg aaaaagcccg tagcgggctg ggaaaggggg aggggatgtt gtctacatgg ctctgctgta cagcggtcct gatcaatcgt caccctttct cggtccttca ccttttcgcc aatccatcga caatcaccgc gagtccctgc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg gccgacctcg ttcaacaggt ccagggcggc acggatcact catgcttgac actttatcac tgataaacat aatatgtcca aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga atacccctga tcgtaattct gagcactgtc gcgctcgacg atgccggtgc tgccgggcct cctgcgcgat ctggttcact tatggcattc tgctggcgct gtatgcgttg gtgcaatttg gcgctgtcgg atcgtttcgg gcggcggcca atcttgctcg tctggggaac cctgtggttg gcatgcacat acaaatggac gcccttttaa atatccgatt attctaataa acgctctttt tatatcctgt caaacactga tagtttaaac tgaaggcggg gcggagaatt aagggagtca cgttatgacc cccgccgatg
10321 agccgtttta cagccgcggg 10381 tttctggagt gttcaaaagt 10441 cgcctaaggt ctgacgttcc 10501 ataaattccc atcgaggggg 10561 atctaccatg aggcggacat 10621 gccggcggtc acttccgtac 10681 cgagccgcag agcgctatcc 10741 ctggctcgtc gtccctggaa 10801 ggcacgcaac cccgccacca 10861 gcggacggga aggcacaggg 10921 cttcaagagc gcatgcacga 10981 ggcgctcgga acgggaactg 11041 gcatgacgtg gtccggtcct 11101 gcccgtcacc atttggcaat 11161 aaagtttctt taatttctgt 11221 tgaattacgt atgagatggg 11281 tttttatgat aaaatatagc 11341 gcgcaaacta cgggcctcct 11401 gtcaatgctg tggtggctct 11461 gagggtggcg tggctctggt 11521 tccggtgatt gaccgaaaat 11581 gccgatgaaa cgctactgat 11641 tacggtgctg tggtaatggt 11701 gctactggtg cggtgataat 11761 tcacctttaa ggttgaatgt 11821 cgcccttttg gccgattcat 11881 taatgcagct caacgcaatt 11941 aatgtgagtt tccggctcgt 12001 atgttgtgtg tgaccatgat 12061 tacgccaagc cgtccgtcaa
cgtttggaac tgacagaacc gcaacgttga aggagccact ttaatgagct aagcacatac gtcagaaacc attattgcgc cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctcggtatcc aattagagtc tcatattcac tctcaactcg agcccagaac gacgcccggc cgacatccgc cgtgccaccg tgcaccatcg tcaaccacta catccagaca agcacggtca gaaccgcagg agtggacgga cgacctcgtc cgtctgcggg gccgaggtgg acggcgaggt cgccggcatc gcctacgcgg gcctacgact ggacggccga gtcgaccgtg tacgtctccc ctgggctcca cgctctacac ccacctgctg aagtccctgg gtggtcgctg tcatcgggct gcccaacgac ccgagcgtgc tatgcccccc gcggcatgct gcgggcggcc ggcttcaagc ggtttctggc agctggactt cagcctgccg gtgccgcccc gaaatctgat gacccctaga gtcaagcaga tcgttcaaac aagattgaat cctgttgccg gtcttgcgat gattatcata taagcatgta ataattaaca tgtaatgcat gacgttattt tagagtcccg caattataca tttaatacgc gatagaaaac ggataaatta tcgcgcgcgg tgtcatctat gttactagat gcggcggctc tggtggtggt tctggtggcg gctctgaggg gttctgaggg tggcggctct gagggaggcg gttccggtgg ttgattatga aaagatggca aacgctaata agggggctat acgcgctaca gtctgacgct aaaggcaaac ttgattctgt ctatcgatgg tttcattggt gacgtttccg gccttgctaa attttgctgg ctctaattcc caaatggctc aagtcggtga tgaataattt ccgtcaatat ttaccttccc tccctcaatc tctttggccc aatacgcaaa ccgcctctcc ccgcgcgttg ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg agctcactca ttaggcaccc caggctttac actttatgct gaattgtgag cggataacaa tttcacacag gaaacagcta ttgcatgcct gcaggtcgac tctagaggat ccccgggtac
12121 catggtggag tctcagaaga 12181 ccaaagggca tcggattcca 12241 ttgcccagct gctcctacaa 12301 atgccatcat acagtggtcc 12361 caaagatgga caaccacgtc 12421 ttcaaagcaa cacaatccca 12481 ctatccttcg agaggacctc 12541 gacctcaaca tctacttcta 12601 ttgcagcaat ttttcaccat 12661 ttacgaacga tctcgcaatc 12721 ttctgtttct gtcacgatga 12781 cggtgattcc ggaatggtac 12841 aacggaggga ttgtgctctg 12901 cgagcttaga agatcgtatt 12961 ggtccccaaa tagttctgtg 13021 tttaaacatt ctagaatcga 13081 ggcatggata ttggaaaaaa 13141 tttgagcact agcttgatcc 13201 gacagtagat agaccgagag 13261 agttctattc gtgaaatttg 13321 ggtatttaac ttgattcctg 13381 gttgaagaca ttctgaatgc 13441 ctttgctgag gggactgcat 13501 tctacttgct ttccagctga 13561 tgtttttacg tctgcaggcg 13621 ttcgcttctg gccggcaaaa 13681 cgatcgtaag cggatacaat 13741 tgcttggaat acttgttggt 13801 cgctagcctt caaactgtaa 13861 tccaatatct atgcatggga
cacgacacac ttgtctactc caaaaatatc aaagatacag attgagactt ttcaacaaag ggtaatatcc ggaaacctcc atctgtcact ttattgtgaa gatagtggaa aaggaaggtg tgcgataaag gaaaggccat cgttgaagat gcctctgccg cccccaccca cgaggagcat cgtggaaaaa gaagacgttc gtggattgat gtgatatctc cactgacgta agggatgacg caagaccctt cctctatata aggaagttca tttcatttgg caacatatac aaaacaaacg aatctcaagc aatcaagcat ttaaatcatt tcttttaaag caaaagcaat tttctgaaaa tagccgggga tggcattagg agacttaatg gtgtctcggt ttggtctcca atcaccgtta cgacgaagac tgtgtctcta cggaggaagg attctgaggc taagagcagt agtagctacg gccgccaccg ccaccagcat ggcttacttg cctcagacta cacgacgcat ccgaggcttc tgctcccttg gggacttctg tggcggctta aagaacgaat gaaaacagga tgtgtagctt actgttgatc cgccggatgt tataaagata tctccttgtg gatccatttt ctatggcacc gcctaaagct ctcgagacaa cagtatgaga gatggcaacc cagggcccgc tataaggttc gaggtgagga agctgtgctt gacttgtcgg aaatatgcaa cattataatg ggcatggtgt gccaaaacct acagctaatg aagagttata cacagtacat tcccttgcca atcagtgaac ccctccatct atgttttcga ctgctctgct gctaggatga cttcatgatt ggggttcttc tggttcctct ggatcctcaa gcttgtgatg tacatgagac acttcctcag agcgttgagt tcttgcctga caacgcccat taaaatggcg ttgaaatggt aaagaaatta tcgatgaatc acttatcgac aggattccag acattgttag gggagttgaa ctggattttc acagcagtga gtgcttcctc atgaactttt ccagagatta ttcagacagg ttccggaatt tcttacttgc tgagagaata atgcggtccg caccctatgc tgcctcctac gcatcaacat catatgtggg
13921 catggctgct caagcacaga 13981 gttccagcca ggcttgatca 14041 tggatctgag tgttacttag 14101 ccagtgccat tgggttcatg 14161 ggctgtggat ttctgcaaac 14221 aacaacgaat ttgcacttga 14281 caagtcatgt tacgatttct 14341 agatagctcg tggctgtcat 14401 tgtggatgga ttggtgtttg 14461 tctcgggcac tgtttctaca 14521 atggctttgt aaataatggg 14581 cagggaggca aacctgaggt 14641 aagggctgct actctaataa 14701 aagtgtggtg acgctatcat 14761 taaaagtctc aggttgccgt 14821 agctcttgca cagcttcata 14881 ttggaagcct aattccatga 14941 tcctgggagt ctagcacgga 15001 tagccaacca cctctgggct 15061 gatgcaagga gaatgatgca 15121 tgacagtgtc ctgtttattc 15181 gcagtgcgtc ttgcaagtct 15241 cggacggcgt cctcgaagcc 15301 cactggccga gcctagctcg 15361 ttcatcctca ggactccccc 15421 ggtcagcccc cattagagtt 15481 caggcctcat taggcgccag 15541 tggatctgaa gtgggcactt 15601 ttctaagcca aaagagaaga 15661 gaaagaaaaa ttagcaagct
gaaatctgtc tttctcagct tccccaactt gttctggacc agtccgtttt ttactgagca actgacagct tttgaggtgt cataagaagc caccggagca gttacctatt gttcttcagg cggtttcgtg ctcttgtact tcttggaaga tttcttgata ctggccttgt ctgttggaat attcccatat gtgctgaagc gagctaagac agatcctggt tttcatatgg acaaaaattc caaattgatc ttgtgaagga tggggggcat acatacttca ggtgcatttc cagaacaacg agctatggct gcttttgttc catcgacgag gccaggaagc atgtcttgaa gctaatttaa cttgaagcat ccagaccaag tgatccacaa ccagaaccat ctttgtcttg gaaagttatg ggaagatttt atggaggccc aatgcttttg aaaagttggc acctctgctt tccgagcccc gcagtttttg ccctgggtac cttgcttgat attgggtttg gaggatgaat ttgatgatga tgaaaagatt agagccgaag ttagatgtag tttcagatgg gagtccactt gtccgagcag cgttttgcct ttggccacaa acagcacctt aagttagccg cagtcaagtt ctttgcttac ttctctacct tcaatagcta gcaacaattg tttctttaca catgagtcct ctgactagag gtggctcgtg agtctaggat ctcaagcagc cctcttggct tctccattat ctgatgattc ttcgttacat tctgattctg agcaatggag cggtccatca gccaagactg ttggataatg cgagctatgt ttgcattagc taaagatcca tctccacgta gtgctttcta ttattggaat cgaacaggtt gttgcgaaac ccaggggaag ctgcaacgac atctcacact ccacttgctg tggtttgata tgcatgcagg taatctgcct ttaagtttta cctagaacaa actacctgag tggattgagg agagtttgtt ctattgggtt cacccgactc aggattggct gatccgcttt cggagtttgc ttccactatc aactatctac ggctggagtt cttcttggtg gtgcggatgc tagtcaagag attgcagcca ttcgcacttg agcatattgc aaaatgccag cactcttcta
15721 caacaataat agacagccct 15781 ccttcaccca tcagagtgtg 15841 gaactatgag ctgacaaagg 15901 aatttcaaag ttgcatcatg 15961 cgatgggtcg agcttgttac 16021 tgggttttct ctgtcgtgga 16081 ctggcaacaa tcacactttg 16141 ggacctggag gtggagttac 16201 agcactttct ctgatggatc 16261 tttgagactc ggcctcatca 16321 gaaagttgaa caaaggtggt 16381 gagtgcatca gggacacata 16441 cctgacgatt gacacgctcc 16501 aataatcgcg aaggggaaca 16561 actagggata cagtgagttg 16621 cctcacattt catttgtctc 16681 catatacacc //
cctattgcca actgggatac aaggtttgaa acgggaacaa ttctctccta ttgtagttgc tgcagacgag aatgaacgga gaagcaactc ttctcaatgg ctttgacaat catgattttc ctctgcctca tcaatgaact tgacgactct ctgctacttg gtccggatat ggaaaaacta tgcaactaag ggtaaacaaa tcaatccagg gtcacaagcc cggtgcccgt gacttgaacg cagtccggtt acctgtatgc ttctggggag acgtcaacag aaagaacagc ttgtcagatc tgttccctct gaatcagaat gcttctcaag tgcacggagg ccaactcgct gctggttttg tatgatgttc ggtcacctga accgcttgtc tgcgcgactc agggtggttg gcctcagttt tcaacctgga cttgaccccg caggcgggtg acatacagtt tcttgacctt agaacaacaa gatgcacaca ggggttcact cacggcctta gctgttcaca agtggatctg caaaacagct cattaaagta ttcagtcttc atccgctact acccatcctt catggctcaa aagattggat catccgtacc aggttctgct agcagctgga gctgctgact cacgacaact cgcaagcaag atgc
Supplementary File S2 LOCUS FEB-2015 SOURCE ORGANISM COMMENT
pBIN19\AKIN10\mC
14196 bp
DNA
circular
11-
This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|725363803| COMMENT VNTDBDATE|725363803| COMMENT LSOWNER| COMMENT VNTNAME|pBIN19 AKIN10 mCherry (355)| COMMENT VNTAUTHORNAME|lala| FEATURES Location/Qualifiers rep_origin complement(7107..7724) /standard_name="oriV" /vntifkey="33" /label=oriV /note="shows similarity to GenBank Accession Number M20134" misc_feature complement(6761..7032) /vntifkey="21" /label=kilA /note="shows similarity to kilA: GenBank Accession Number M62846" CDS complement(6410..6760) /vntifkey="4" /label=NPTIII /note="shows similarity to NPTIII gene: GenBank Accession Number V01547" transposon complement(5640..6409) /vntifkey="44" /label=IS1 /note="shows similarity to transposable element IS1: GenBank Accession Number X58999" misc_feature complement(3165..4646) /vntifkey="21" /label=trfA /note="shows similarity to trfA: GenBank Accession Number X00713" misc_feature complement(2122..3164) /vntifkey="21" /label=tetA /note="shows similarity to tetA: GenBank Accession Number L13842" CDS complement(1404..1534) /vntifkey="4" /label=lacI /note="shows similarity to lacI: GenBank Accession Number J01636" rep_origin complement(1103..1403) /standard_name="M13 ori" /vntifkey="33" /label=M13\ori /note="shows similarity to GenBank Accession Number X02513" CDS complement(11817..11957) /vntifkey="4" /label=lacI /note="shows similarity to lacI: GenBank Accession Number J01636" misc_feature complement(11375..11816)
/vntifkey="21" /label=geneIII /note="shows similarity to geneIII: GenBank Accession Number V00604" repeat_region complement(10081..10242) /vntifkey="34" /label=RB /note="shows similarity to T-DNA right border: GenBank Accession Number J01826" misc_feature complement(8885..10068) /vntifkey="21" /label=tetA /note="shows similarity to tetA: GenBank Accession Number X75761" rep_origin complement(8514..8892) /standard_name="ColE1 ori" /vntifkey="33" /label=ColE1\ori /note="shows similarity to GenBank Accession Number V00268" misc_feature complement(7737..8520) /vntifkey="21" /label=traF /note="shows similarity to traF: GenBank Accession Number X54459" repeat_unit complement(1535..1682) /vntifkey="35" /label=LB CDS complement(4647..5639) /vntifkey="4" /label=NPTIII primer_bind complement(12018..12032) /vntifkey="28" /label=M13rev primer_bind complement(954..970) /vntifkey="28" /label=M13fwd primer_bind 1246..1267 /vntifkey="28" /label=LBa1\primer primer_bind 1445..1466 /vntifkey="28" /label=LBb1\primer CDS 10548..11096 /vntifkey="4" /label=Basta-R misc_feature 7..717 /vntifkey="21" /label=mCherry misc_feature 12501..12543 /vntifkey="21" /label=möglicherweise\kein\XhoI enhancer 12520..12650 /vntifkey="9" /label=TL\(TEV\Leader) promoter 12095..12511 /vntifkey="29" /label=1x35\Promoter misc_feature 12658..14193 /vntifkey="21"
/label=AKIN10\splicing\form\1\(und3) BASE COUNT 3227 a 3961 c 3723 g 3285 t ORIGIN 1 ggccgcatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 61 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 121 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc 181 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg 241 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg 301 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg 361 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc 421 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag 481 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac 541 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac 601 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag 661 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaatct 721 agagtccgca aaaatcacca gtctctctct acaaatctat ctctctctat tttctccaga 781 ataatgtgtg agtagttccc agataaggga attagggttc ttatagggtt tcgctcatgt 841 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa 901 aatttctaat tcctaaaacc aaaatccagt gacctactag tgagctcgaa ttcactggcc 961 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 1021 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 1081 caacagttgc gcagcctgaa tggcgcccgc tcctttcgct ttcttccctt cctttctcgc 1141 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 1201 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg 1261 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 1321 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt 1381 ataagggatt ttgccgattt cggaaccacc atcaaacagg attttcgcct gctggggcaa 1441 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg 1501 ttgcccgtct cactggtgaa aagaaaaacc accccagtac attaaaaacg tccgcaatgt 1561 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag 1621 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
1681 agtccgggac tcatgttacc 1741 gatgctattc gatctcgcgg 1801 agggtagcat aatatcatct 1861 ccctcgcaga cgtgacaggc 1921 tgtcgatctt cgctgaggaa 1981 gctgagtggc ctatccattg 2041 ctcaccgaat tgcatccccg 2101 gctgatcgac gtaggttcga 2161 gtcgcgagat aggccgagcc 2221 acgccaggcc aacactaaag 2281 ctactggaac gtgagcagag 2341 gcacgggagg accgcccccg 2401 ccaggcccgc accaacagcg 2461 ccacgcccgc gggctaccta 2521 gcagagcggc gtcgccgcga 2581 ccccgcccgg gaaatattaa 2641 gtgcgccgag cggacgatca 2701 tcacgagcaa cctcggcctc 2761 gctgttcggg ttggggccgt 2821 cctcctgttt ccgaatgcca 2881 cggcatctcg tgctcgtaaa 2941 cggacccgaa cggaaatcct 3001 gcacgtcggc aattttaatc 3061 ctctgtttat gagcgaagca 3121 agtgcgtcga ctgctgaacc 3181 cccagccgga catcattgac 3241 ccaggcgtgt cgacctgctc 3301 gcgccacttc tttccagctt 3361 gagcgggtac ccgtcggcga 3421 cagcttgcgg acagcacgac
ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc ggaagaacgg caactaagct gccgggtttg aaacacggat gttgattgta acgatgacag agcgttgctg cctgtgatca gatccgaatt atcagccttc ttattcattt ctcgcttaac gagaactatg ccgacataat aggaaatcgc tggataaagc gctatttctt tagaagtgaa cgttgacgat atcaactccc ggtacaggtc ggggacccga agttccgact gtcggcctga cccagatctg gggctgagaa agcccagtaa ggaaacaact cccccggaac caaaggaagt aggttaaacc cgctccgatc gagaacattg gttcctgtag gcatcgggat tggcggatca gagcagaagt cctccggccg ccagttgcca ggcggtaaag ttgccacttg cgggtcagca cggttccgaa cgccatggaa tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac agttccgcaa atagccccca ggaccgccat caatcgtatc agagatgaac acgaccatca gcggctgcac agcgcctacc caggcggtag accgaaataa acaacaagct ccagaatagc gatgaagatg cgcatccacc agattcccgt tggaatctgt taaacccgcc ggcaacgccc gcagcagcat accggcgacc ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc gaagaccgac agcccaatga tctcgccgtc gatgtaggcg caaccgttca gcgaacgcct ccatgggctt tttctcctcg catctctgga gctttcttca gggccgacaa tcggatctcg cgctccaagc cgtcgaatct gagccttaat cacaattgtc cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg actgacccca caaggcccta gcgtttgcaa tgcaccaggt tccaccaggc cgctgcctcg caactcttcg caggcttcgc ttcacgcggg tggaatccga tccgcacatg aggcggaagg ggctcccggt gcgagctgaa atagtcgaac atccgtcggg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa
3481 gatttcctcg ccaggacgcg 3541 gaagcggtgc tgaagcccat 3601 cgccgtcgcc ggccattgat 3661 cgaccagccc cgccgatagg 3721 ggtgcgcttc cggcccgcag 3781 ctcgacgccg ccttgttttg 3841 cagcgcctcg gggccgtgtc 3901 gtttggcatc aaagctgcat 3961 ttccttgatc cgctgacctg 4021 ttttgccagg ctcgcgtgtc 4081 gatggtcatc aacgctccac 4141 ggcggccgat gctcgatctt 4201 ggccgtagct gcgcacgcat 4261 gacggtgcgg cgatcagttc 4321 ttgcctgtat ggattgcccc 4381 gactcacgcc ccttggtgtc 4441 cagataatcc cgtccttctc 4501 gtacttggta ccaaatactt 4561 gccgtgggcc tgcgctcctg 4621 cttgtcgccg agtaaaatat 4681 aatattttat agctcgacat 4741 actgttcttc tcataccact 4801 tgtccgccct tctttcacaa 4861 agatgttgct ggcttttccg 4921 tctttaaaaa tcccagtttt 4981 cgcaatccac aagcggctgt 5041 ctaagctatt ctgatgcact 5101 ccgcatacag tccgagcaaa 5161 ggacgccatc tcaaagtgca 5221 ggacctttgg tcccgttcca
tcgatcagga cctggcaacg ggacgttttc ttgccacggt agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct gcgtactcca acacctgctg ccacaccagt tcgtcatcgt gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct tcctcgccgg cggtttttcg cttcttggtc gtcatagttc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg ggcgcgggca gggcaggggg agccagttgc acgctgtcgc tgctggacca tcgagccgac ggactggaag gtttcgcggg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt gccttccggt caaacgtccg attcattcac cctccttgcg ggggcaatgt gcccttattc ctgatttgac ccgcctggtg accttatcgg caatgaagtc ggtcccgtag accgtctggc ttccgaatct tgccctgcac gaataccagc gaccccttgc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg gcatcgttgc gccacatcta ggtactaaaa caattcatcc tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat cccgatatcc tccctgatcg accggacgca gaaggcaatg gccgcttctc ccaagatcaa taaagccact tactttgcca gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg atcatacagc tcgcgcggat ctttaaatgg agtgtcttct atcggccaga tcgttattca gtaagtaatc caattcggct cgtataggga caatccgata tgtcgatgga gtgaaagagc ctcgataatc ttttcagggc tttgttcatc ttcatactct ggcctcactc atgagcagat tgctccagcc atcatgccgt aacaggcagc tttccttcca gccatagcat catgtccttt
5281 catcataggt ttttcatttt 5341 ctcccaccag acgcagcggt 5401 atttttcgat tattatttcc 5461 ttcctctttt agacgaactc 5521 caattcactg tttcaaagtt 5581 gttttcaaag cacaattatg 5641 ggtgatgctg ctccagtggc 5701 ttctgtgtct gtaacggcaa 5761 aagcaccgcc ggcaactgca 5821 gttcacttac tggccatgaa 5881 tggcgttgga cgattttacg 5941 tcacttaaaa cagtgacgtc 6001 atcgtctgcg gcgccagcgc 6061 tggctgtttt attcggtgaa 6121 cgcactatgg cgtggtgata 6181 tggatgacgg gcacgtaatc 6241 agcaagcgat gcacctggca 6301 cggctgggac caaagtcatc 6361 gggcattatc attatgatag 6421 aatttacaag caagttttta 6481 tgctttgccc ccccctgaaa 6541 tccttacata tattgtcaat 6601 atattcaagg agctttttaa 6661 atatggcgct acctcggtat 6721 aatcttacct cacgagcacg 6781 gcacccgcga cgccatgaag 6841 tccgtgaatg gccgccctca 6901 ctgcccggca aatgcttccg 6961 ggcgtcgcgc aatggcaagg 7021 actgccagcg cagcccctgg
ggtcccttta taccggctgt ccgtcatttt taaatatagg cttatatacc ttagcaggag acattccttc cgtatctttt cagttttttc aattccggtg atattctcat tttagccatt ctacagtatt taaagatacc ccaagaagct aattataaca ttccttgcat tctaaaacct taaataccag aaaacagctt ttggcgtata acatagtatc gacggagccg attttgaaac ccaacttact gatttagtgt atgatggtgt ttttgaggtg atcagctgtc cctcctgttc agctactgac ggggtggtgc ggacatcagc gctatctctg ctctcactgc cgtaaaacat accgcttctc aacccggtac gcaccagaaa atcattgata tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca aactcaggcc gcagtcggta acctcgcgca tacagccggg cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc acgcgtatga cagtctccgg aagacggttg ttgcgcacgt cgacgctggg gcgtcttatg agcctgctgt caccctttga atggctggcc gctgtatgaa tcccgcctga agggaaagct atacgcagcg aattgagcgg cataacctga atctgaggca ggaagtcgct gtcgttctca aaatcggtgg agctgcatga tgaacataaa acactatcaa taagttggag tcattaccca ctataaggtt attgtcctgg gtttcaagca ttagtccatg attctataga tatattgata agcgcgctgc ctatgccttg cggcgatatc ttctatataa aagatatatt atcttatcag caatctgcct cctcatcctc ttcatcctct tcgtcttggt tcatagagta attctgtaaa ggtccaattc tcgttttcat atcacctcaa atggttcgct gggtttatcg cacccccgaa ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc cctggtcgct gaatgtcgat gccagcacct gcggcacgtc tcgggctgat cgcccatccc gttactgccc cgatcccggc ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg
7081 ggggatggga cacccccctt 7141 cggcgtgcgc ttataaatat 7201 tggtttaaaa ggaaaccctt 7261 gcaaatgctg gcgcccctca 7321 tctgtcagca cagtagtcgc 7381 gcccctcaag tcatctgtgg 7441 gaaactcgcg tccacgtcgc 7501 cggccgaaat cggcccctca 7561 agtgtcaacg ggtatccaca 7621 acgccggcgg cgtttgcagg 7681 gccatagacg tcgcaaaggc 7741 gctcggtctt tcgctcttct 7801 tgatggagcg ttttagcggc 7861 taaaaaagtc gccaagctcg 7921 tcctgcttct gaaccgcgcc 7981 gtgcgcgggt caggtcgcca 8041 ttgatgcggg tttgtagccc 8101 tggccgacgg cttttcctca 8161 atcgctcttc cttcctggtt 8221 ggcttggttt agccggccag 8281 cctcgcagag agtgaagaag 8341 gaacacccgc ccgttggata 8401 caccaaggaa gaaaaaggat 8461 ggatataccg ggaaaagcgc 8521 cacgcttccc tcggaacagg 8581 agagcgcacg ctgtcgggtt 8641 tcgccacctc ggagcctatg 8701 gaaaaacgcc cttttgctca 8761 catgttcttt cctttgagtg 8821 agctgatacc gcgaggaagc
ggcccgcgtt agcgggccgg gagggttcga gaaggggggg ggtcacgcgc acagggcgca gccctggtta aaaacaaggt gcaggttaaa agacaggtta gcggtggccg aaaaacgggc gattttctgc ctgtggacag cccctcaaat gtcaataggt ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt tgtcaatacc gcagggcact tatccccagg cttgtccaca taaaatcagg cgttttcgcc gatttgcgag gctggccagc cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt tccgcccctc atctgtcagt gagggccaag ttttccgcga gcgcggtgtc tcgcacacgg cttcgacggc gtttctggcg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg gccttgctcg tcggtgatgt acttcaccag ctccgcgaag catggggacg tgcttggcaa tcacgcgcac cccccggccg atggctctgc cctcgggcgg accacgccca tcatgacctt cttcgatctt cgccagcagg gcgaggatcg tggcatcacc cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc ccagctcgcg gacgtgctca tagtccacga cgcccgtgat ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc gttcgtctgg aaggcagtac accttgatag gtgggctgcc catcagccat ccgcttgccc tcatctgtta cgccggcggt caggattccc gttgagcacc gccaggtgcg aataagggac tcgcgggtgg gcctacttca cctatcctgc ccggctgacg agtctacacg aaccctttgg caaaatcctg tatatcgtgc aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc gaagggagaa aggcggacag gtatccggta agcggcaggg agggagcttc cagggggaaa cgcctggtat ctttatagtc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cctgcgttat cccctgattc tgtggataac cgtattaccg gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
8881 ggaagagcgc ggacgctagg 8941 gcagggcatg ggaaaggggg 9001 aggggatgtt cagcggtcct 9061 gatcaatcgt ccttttcgcc 9121 aatccatcga ggcttcgtcg 9181 aaggcgtcta ggtgccgccg 9241 cgctcgccgg agtgaagtag 9301 ctgattgtca gcagaggaag 9361 cgaagctgcg ggcatggatg 9421 cgcgcgccat cccgatcaga 9481 aatgagcgcc cgccagcatg 9541 gcttcggcca aagcgccggc 9601 tgctgaaccc gccgacctcg 9661 ttcaacaggt catgcttgac 9721 actttatcac aatccgcgcg 9781 ttcaatcgga atacccctga 9841 tcgtaattct atgccggtgc 9901 tgccgggcct tatggcattc 9961 tgctggcgct gcgctgtcgg 10021 atcgtttcgg tctggggaac 10081 cctgtggttg gcccttttaa 10141 atatccgatt tatatcctgt 10201 caaacactga gcggagaatt 10261 aagggagtca cgtttggaac 10321 tgacagaacc ttaatgagct 10381 aagcacatac cactatcagc 10441 tagcaaatat ctcggtatcc 10501 aattagagtc agcccagaac 10561 gacgcccggc tgcaccatcg 10621 tcaaccacta gaaccgcagg
cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg caccctttct cggtccttca acgttcctga caacgagcct caatcaccgc gagtccctgc tcgaacgctg cgtccggacc tcgcggcccg caacagcggc gagagcggag cctgttcaac catcgctgtc gccggcctgc tcctcaagca cggccccaac tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt agtcgtcgtc ggctctcggc accgaatgcg tatgattctc gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac ccagggcggc acggatcact gtattcggct gcaactttgt tgataaacat aatatgtcca ccaacttatc agtgataaag ccagcggagg ctggtccgga ggccagacgt gaaacccaac gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt cctgcgcgat ctggttcact cgaacgacgt caccgcccac gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc gcggcggcca atcttgctcg tctcgctggc cggcgccaga gcatgcacat acaaatggac gaacggataa accttttcac attctaataa acgctctttt ctcttaggtt tacccgccaa tagtttaaac tgaaggcggg aaacgacaat ctgatcatga cgttatgacc cccgccgatg acgcgggaca agccgtttta gcaacgttga aggagccact cagccgcggg tttctggagt gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc tcatattcac tctcaactcg atcgaggggg atctaccatg cgacatccgc cgtgccaccg aggcggacat gccggcggtc catccagaca agcacggtca acttccgtac cgagccgcag
10681 agtggacgga gccgaggtgg 10741 acggcgaggt gcctacgact 10801 ggacggccga ctgggctcca 10861 cgctctacac gtggtcgctg 10921 tcatcgggct tatgcccccc 10981 gcggcatgct ggtttctggc 11041 agctggactt gaaatctgat 11101 gacccctaga aagattgaat 11161 cctgttgccg taagcatgta 11221 ataattaaca tagagtcccg 11281 caattataca ggataaatta 11341 tcgcgcgcgg gcggcggctc 11401 tggtggtggt gttctgaggg 11461 tggcggctct ttgattatga 11521 aaagatggca acgcgctaca 11581 gtctgacgct ctatcgatgg 11641 tttcattggt attttgctgg 11701 ctctaattcc tgaataattt 11761 ccgtcaatat tctttggccc 11821 aatacgcaaa ggcacgacag 11881 gtttcccgac agctcactca 11941 ttaggcaccc gaattgtgag 12001 cggataacaa ttgcatgcct 12061 gcaggtcgac acacacttgt 12121 ctactccaaa agacttttca 12181 acaaagggta gtcactttat 12241 tgtgaagata ataaaggaaa 12301 ggccatcgtt cacccacgag 12361 gagcatcgtg attgatgtga 12421 tatctccact acccttcctc
cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc cgccggcatc gcctacgcgg gtccctggaa ggcacgcaac gtcgaccgtg tacgtctccc cccgccacca gcggacggga ccacctgctg aagtccctgg aggcacaggg cttcaagagc gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg cagcctgccg gtgccgcccc gtccggtcct gcccgtcacc gtcaagcaga tcgttcaaac atttggcaat aaagtttctt gtcttgcgat gattatcata taatttctgt tgaattacgt tgtaatgcat gacgttattt atgagatggg tttttatgat tttaatacgc gatagaaaac aaaatatagc gcgcaaacta tgtcatctat gttactagat cgggcctcct gtcaatgctg tctggtggcg gctctgaggg tggtggctct gagggtggcg gagggaggcg gttccggtgg tggctctggt tccggtgatt aacgctaata agggggctat gaccgaaaat gccgatgaaa aaaggcaaac ttgattctgt cgctactgat tacggtgctg gacgtttccg gccttgctaa tggtaatggt gctactggtg caaatggctc aagtcggtga cggtgataat tcacctttaa ttaccttccc tccctcaatc ggttgaatgt cgcccttttg ccgcctctcc ccgcgcgttg gccgattcat taatgcagct tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt caggctttac actttatgct tccggctcgt atgttgtgtg tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagaggat ccccgggtac cgtcaacatg gtggagcacg aatatcaaag atacagtctc agaagaccaa agggcaattg atatccggaa acctcctcgg attccattgc ccagctatct gtggaaaagg aaggtggctc ctacaaatgc catcattgcg gaagatgcct ctgccgacag tggtcccaaa gatggacccc gaaaaagaag acgttccaac cacgtcttca aagcaagtgg gacgtaaggg atgacgcaca atcccactat ccttcgcaag
12481 tatataagga atatacaaaa 12541 caaacgaatc atcatttctt 12601 ttaaagcaaa cgggcccatg 12661 gatggatcag ttacaagctt 12721 gggagaactc tgcattgaca 12781 ggacataagg ggagatggag 12841 gagaaagtga catcatccgt 12901 ctctatgagg tgtgaactct 12961 ggtgagctat ggcgaggaac 13021 ttttttcagc ggttcacaga 13081 gacctcaagc tgctgatttt 13141 ggcctgagca aagtccaaat 13201 tatgccgctc agatgtctgg 13261 agctgtggtg tgatgaaaac 13321 attcccaacc ccatttatct 13381 cctggtgcta acgagtaacc 13441 atccctgaga tttagctgtt 13501 cctcctccag ccaagaagtt 13561 atcaatatgg aacccagaat 13621 gatggcactg tagtggttat 13681 ctcggggctg agcagaaagc 13741 gttgcttcac agttggcttg 13801 agatctcaat ggctcatccc 13861 cgtgaaataa ttggaagaag 13921 atagggcact tggtatgctc 13981 agtaactcga agagaacgaa 14041 gcagctgtta aactcgggac 14101 gacaagtatc cttggatctg 14161 tgtgctgctt //
agttcatttc atttggagag gacctcgacc tcaacacaac tcaagcaatc aagcattcta cttctattgc agcaatttaa agcaattttc tgaaaatttt caccatttac gaacgatagc gcacaggcag tagaagtggg gtagaatcga ttctaccaaa ttggtattgg ttcctttggt agggtgaaga tagctgagca ttgctatcaa gatcctcaat cgtcgcaaaa tcaagaacat ggagagagat caaaatcttg agactattta tgcatcctca ttatagagac tcccacagat atttatcttg tcatggagta ttgactatat tgttgagaag ggtagattgc aggaggatga agataatatc aggagtggaa tactgccatc gaaacatggt ctgaaaactt gcttttggac tctaaatgca atgtaaagat acataatgcg agatggtcat tttttgaaga caagttgtgg cagaggtaat ttcgggcaag ttatatgctg gccctgaagt tgatactcta cgctcttctc tgtgggacgc ttccatttga tttttaagaa gataaaggga gggatataca cattacctag gagatttgat cccccggatg cttgtagttg accccatgaa tccggcaaca cccttggttc caagctcatc ttccgaggta atactgtgca acaggcaaaa aagattgacg aggagattct gatttgacag aaaccacctc atcgaatcgc tccgcaaccg tgacgtacta tctgatactg gacaatcgtt tccgtgcctc agtttcaaga gaccatggaa ggtactcccc gtatgcatcc ctgttagcca tcggcttcca ggactgatgg aatatcaagg accctgttga gagaaaatgg gctcttggac ttcagtctcg tgacggaagt cctgaaagcc ctgcaagatt tgaatgtatg acaacatgaa gtgcagatgg gttcctaaca gcagcgcaga tgcacgataa caactacttt ggagacgagt ccagcataat agtcgcccaa tgttgtcaag tttgaaattc agttgtataa tactggattt gcagagagta caaggtcctc agttcttgtt ttcttgctca gctccgagtc ctctgc
Supplementary File S3 LOCUS JUL-2015 SOURCE ORGANISM COMMENT
Atraptor1b\YFP\i
9501 bp
DNA
circular
1-
This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|738342882| COMMENT VNTDBDATE|738342882| COMMENT LSOWNER| COMMENT VNTNAME|Atraptor1b YFP in pQE80L fw (ML742)| COMMENT VNTAUTHORNAME|Bernhard Wurzinger| FEATURES Location/Qualifiers misc_feature 6..4040 /vntifkey="21" /label=Atraptor1b misc_feature 8775..9500 /vntifkey="21" /label=YFP promoter 8631..8711 /vntifkey="30" /label=T5\promoter/lac\operator\element misc_feature 8685..8685 /vntifkey="21" /label=T5\transcription\start misc_feature 8751..8768 /vntifkey="21" /label=6x\His\tag\coding\sequence terminator 4081..4175 /vntifkey="43" /label=lambda\t0\transcriptional\termination\region terminator 4937..5034 /vntifkey="43" /label=rrnB\T1\transcriptional\termination\region misc_feature complement(5125..6206) /vntifkey="21" /label=lacI rep_origin 6801..6801 /vntifkey="33" /label=ColE1\origin\of\replication misc_feature complement(7559..8419) /vntifkey="21" /label=Beta-lactamase\CDS\(AmpR) misc_feature complement(5122..6081) /vntifkey="21" /label=lac\repressor\true\CDS BASE COUNT 2353 a 2347 c 2367 g 2434 t ORIGIN 1 ccgggatggc attaggagac ttaatggtgt ctcggttctc gcaatcttct gtttctttgg 61 tctccaatca ccgttacgac gaagactgtg tctctagtca cgatgacggt gattcccgga 121 ggaaggattc tgaggctaag agcagtagta gctacgggaa tggtacaacg gagggagccg 181 ccaccgccac cagcatggct tacttgcctc agactattgt gctctgcgag cttagacacg 241 acgcatccga ggcttctgct cccttgggga cttctgagat cgtattggtc cccaaatggc 301 ggcttaaaga acgaatgaaa acaggatgtg tagctttagt tctgtgttta aacattactg
361 ttgatccgcc tggatagatc 421 cattttctat agcactcagt 481 atgagagatg gtagatgagg 541 tgaggaagct ctattccatt 601 ataatgggca tttaacaaga 661 gttatacaca aagacaccct 721 ccatctatgt gctgagcttc 781 atgattgggg cttgctgctt 841 gtgatgtaca tttacgtctt 901 gcctgacaac cttctgaaag 961 aaattatcga cgtaagacat 1021 tgttagggga tggaatgtgc 1081 ttcctcatga agccttttcc 1141 ggaatttctt atatctcacc 1201 ctatgctgcc gctgctgaaa 1261 tctgtctttc cagccaagtc 1321 cgttttttac tctgagcata 1381 agaagccacc tgccatcggt 1441 ttcgtgctct gtggatctgg 1501 ccttgtctgt acgaatgagc 1561 taagacagat tcatgtcaaa 1621 ttgatcttgt agctcgggtg 1681 catttccaga gatggacatc 1741 gacgaggcca gggcaccttg 1801 aagcatccag ctttgtcttt 1861 gtcttggaaa gaggcaaatg 1921 cttttgaaaa gctgctgcag 1981 tttttgccct gtggtggagg 2041 atgaatttga agtctcttag 2101 atgtagtttc cttgcacgtt
ggatgttata aagatatctc cttgtgctag aatcgaggca ggcaccgcct aaagctctcg agacaattgg aaaaaatttg gcaacccagg gcccgctata aggttcagct tgatccgaca gtgcttgact tgtcggaaat atgcaaagac cgagagagtt tggtgtgcca aaacctacag ctaatggtga aatttgggta gtacattccc ttgccaatca gtgaacttga ttcctggttg tttcgactgc tctgctgcta ggatgattct gaatgccttt ttcttctggt tcctctggat cctcaaggga ctgcattcta tgagacactt cctcagagcg ttgagtttcc agctgatgtt gcccattaaa atggcgttga aatggttctg caggcgttcg tgaatcactt atcgacagga ttccaggccg gcaaaacgat gttgaactgg attttcacag cagtgacgga tacaattgct acttttccag agattattca gacaggactt gttggtcgct acttgctgag agaataatgc ggtccgcaaa ctgtaatcca tcctacgcat caacatcata tgtgggatgc atgggacatg tcagcttccc caacttgttc tggacccaag cacagagttc tgagcaactg acagcttttg aggtgtggct tgatcatgga ggagcagtta cctattgttc ttcaggtgtt acttagccag tgtacttctt ggaagatttc ttgatatggg ttcatgggct tggaatattc ccatatgtgc tgaagcttct gcaaacaaca cctggttttc atatggacaa aaattcttgc acttgacaag gaaggatggg gggcatacat acttcatacg atttctagat acaacgagct atggctgctt ttgttctggc tgtcattgtg ggaagcatgt cttgaagcta atttaattgg tgtttgtctc accaagtgat ccacaaccag aaccattgtt tctacaatgg gttatgggaa gattttatgg aggcccaaat aatgggcagg gttggcacct ctgctttccg agccccaacc tgaggtaagg gggtaccttg cttgatattg ggtttgactc taataaaagt tgatgatgaa aagattagag ccgaagacgc tatcattaaa agatgggagt ccacttgtcc gagcagaggt tgccgtagct
2161 ttgcctttgg aagcctcagt 2221 caagttcttt gggagtgcaa 2281 caattgtttc caaccagtgg 2341 ctcgtgagtc caaggatctc 2401 cattatctga agtgtcagca 2461 atggagcggt tgcgtccgag 2521 ctatgtttgc cggcgtgtgc 2581 tttctattat ggccgaccag 2641 gggaagctgc tcctcatggt 2701 ttgatatgca agccccccta 2761 gaacaaacta cctcatctat 2821 tgggttcacc tctgaacgga 2881 gtttgcttcc aagccacttc 2941 ttggtggtgc gaaaaattcg 3001 cacttgagca aataatccta 3061 ttgccaactg cacccattct 3121 ctcctattgt tatgaggaag 3181 caactcttct tcaaagctct 3241 gcctcatcaa gggtcggtcc 3301 ggatatggaa ttttcttcaa 3361 tccagggtca caacaacagt 3421 ccggttacct ctggagaaag 3481 aacagcttgt ctttctgctt 3541 ctcaagtgca agactctatg 3601 atgttcggtc gttgaaaggg 3661 tggttggcct gcatcacagg 3721 cgggtgacat acgattgatg 3781 cacacagggg atcgcgagtg 3841 gatctgcaaa gggataatcc 3901 gctactaccc acatttcatc
ccacaaacag caccttaagt tagccgcagc ttcatattgg gcttacttct ctaccttcaa tagctaaatt ccatgatcct tttacacatg agtcctctga ctagagctag cacggatagc taggatctca agcagccctc ttggctcctc tgggctgatg tgattcttcg ttacattctg attctggaat gatgcatgac ccatcagcca agactgttgg ataatgctgt ttattcgcag attagctaaa gatccatctc cacgtattgc aagtctcgga tggaatcgaa caggttgttg cgaaaccctc gaagcccact aacgacatct cacactccac ttgctggcct agctcgttca tgcaggtaat ctgcctttaa gttttaggac tcccccggtc cctgagtgga ttgaggagag tttgttcatt agagttcagg cgactcagga ttggctgatc cgcttttagg cgccagtgga actatcaact atctacggct ggagttgtgg gcacttttct ggatgctagt caagagattg cagccaaaag agaagagaaa tattgcaaaa tgccagcact cttctattag caagctcaac ggatacaagg tttgaaacgg gaacaaagac agccctcctt agttgctgca gacgagaatg aacggatcag agtgtggaac caatggcttt gacaatcatg attttcctga caaaggaatt tgaacttgac gactctctgc tacttgttgc atcatgcgat aaactatgca actaagggta aacaaaagct tgttactggg caagcccggt gcccgtgact tgaacgctgt cgtggactgg gtatgcttct ggggagacgt caacagtcac actttgggac cagatctgtt ccctctgaat cagaatgtgg agttacagca cggaggccaa ctcgctgctg gttttgctga tggatctttg acctgaaccg cttgtctgcg cgactcggcc tcatcagaaa cagttttcaa cctggacttg accccgcaaa ggtggtgagt acagtttctt gaccttagaa caacaaggga cacatacctg ttcactcacg gccttagctg ttcacagaca cgctccaata acagctcatt aaagtattca gtcttcaagg ggaacaacta atccttcatg gctcaaaaga ttggatcagt gagttgcctc
3961 cgtaccaggt tacacccacg 4021 acaactcgca gctgagcttg 4081 gactcctgtt ttcagaacgc 4141 tcggttgccg gagattttca 4201 ggagctaagg tgatatatcc 4261 caatggcatc tacctataac 4321 cagaccgttc taagcacaag 4381 ttttatccgg ggaatttcgt 4441 atggcaatga ttacaccgtt 4501 ttccatgagc cgatttccgg 4561 cagtttctac ggcctatttc 4621 cctaaagggt gagtttcacc 4681 agttttgatt caccatgggc 4741 aaatattata tcatcatgcc 4801 gtttgtgatg ctgcgatgag 4861 tggcagggcg gcctggggta 4921 atgactctct actgggcctt 4981 tcgttttatc cgccctctag 5041 attacgtgca agtgagctaa 5101 cttacattaa gtcgtgccag 5161 ctgcattaat gcgccagggt 5221 ggtttttctt cctggccctg 5281 agagagttgc cctgtttgat 5341 ggtggttaac ccactaccga 5401 gatatccgca ccagcgccat 5461 ctgatcgttg tttgcatggt 5521 ttgttgaaaa gctgaatttg 5581 attgcgagtg cagaacttaa 5641 tgggcccgct ccacgcccag 5701 tcgcgtaccg cagagacatc
tctgctagca gctggagctg ctgactcatt tgtctccata agcaagatga cccgggtcga cctgcagcca agcttaatta gatagatcca gtaatgacct cagaactcca tctggatttg ccgggcgttt tttattggtg agaatccaag ctagcttggc aagctaaaat ggagaaaaaa atcactggat ataccaccgt gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg agctggatat tacggccttt ttaaagaccg taaagaaaaa cctttattca cattcttgcc cgcctgatga atgctcatcc aagacggtga gctggtgata tgggatagtg ttcacccttg aaactgaaac gttttcatcg ctctggagtg aataccacga acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ttattgagaa tatgtttttc gtctcagcca atccctgggt taaacgtggc caatatggac aacttcttcg cccccgtttt cgcaaggcga caaggtgctg atgccgctgg cgattcaggt gcttccatgt cggcagaatg cttaatgaat tacaacagta gggcgtaatt tttttaaggc agttattggt gcccttaaac agcttgaggc atcaaataaa acgaaaggct cagtcgaaag tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc gtcgatgata agctgtcaaa catgagaatt gtgcctaatg ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ttcaccagtg agacgggcaa cagctgattg cccttcaccg agcaagcggt ccacgctggt ttgccccagc aggcgaaaat ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc gcaaccagca tcgcagtggg aacgatgccc tcattcagca ccggacatgg cactccagtc gccttcccgt tccgctatcg agatatttat gccagccagc cagacgcaga cgcgccgaga aacagcgcga tttgctggtg acccaatgcg accagatgct tcttcatggg agaaaataat actgttgatg ggtgtctggt
5761 aagaaataac cctggtcatc 5821 cagcggatag gcaccgccgc 5881 tttacaggct cacccagttg 5941 atcggcgcga ccagactgga 6001 ggtggcaacg cgcggttggg 6061 aatgtaattc cagaaacgtg 6121 gctggcctgg actctgcgac 6181 atcgtataac ccgggcgcta 6241 tcatgccata gggcagcgtt 6301 gggtcctggc tgatgacggt 6361 gaaaacctct agcggatgcc 6421 gggagcagac gggcgcagcc 6481 atgacccagt gcatcagagc 6541 agattgtact gtaaggagaa 6601 aataccgcat tcggtcgttc 6661 ggctgcggcg acagaatcag 6721 gggataacgc aaccgtaaaa 6781 aggccgcgtt cacaaaaatc 6841 gacgctcaag gcgtttcccc 6901 ctggaagctc tacctgtccg 6961 cctttctccc tatctcagtt 7021 cggtgtaggt cagcccgacc 7081 gctgcgcctt gacttatcgc 7141 cactggcagc ggtgctacag 7201 agttcttgaa ggtatctgcg 7261 ctctgctgaa ggcaaacaaa 7321 ccaccgctgg agaaaaaaag 7381 gatctcaaga aacgaaaact 7441 cacgttaagg atccttttaa 7501 attaaaaatg tctgacagtt
gccggaacat tagtgcaggc agcttccaca gcaatggcat ttaatgatca gcccactgac gcgttgcgcg agaagattgt tcgacgccgc ttcgttctac catcgacacc accacgctgg gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccaatcagca acgactgttt gcccgccagt tgttgtgcca agctccgcca tcgccgcttc cactttttcc cgcgttttcg ttcaccacgc gggaaacggt ctgataagag acaccggcat gttactggtt tcacattcac caccctgaat tgactctctt ccgcgaaagg ttttgcacca ttcgatggtg tcggaatttc cacgggtgcg catgatctag agctgcctcg cgcgtttcgg gacacatgca gctcccggag acggtcacag cttgtctgta aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg cacgtagcga tagcggagtg tatactggct taactatgcg gagagtgcac catatgcggt gtgaaatacc gcacagatgc caggcgctct tccgcttcct cgctcactga ctcgctgcgc agcggtatca gctcactcaa aggcggtaat acggttatcc aggaaagaac atgtgagcaa aaggccagca aaaggccagg gctggcgttt ttccataggc tccgcccccc tgacgagcat tcagaggtgg cgaaacccga caggactata aagataccag cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg cgttcgctcc aagctgggct gtgtgcacga accccccgtt atccggtaac tatcgtcttg agtccaaccc ggtaagacac agccactggt aacaggatta gcagagcgag gtatgtaggc gtggtggcct aactacggct acactagaag gacagtattt gccagttacc ttcggaaaaa gagttggtag ctcttgatcc tagcggtggt ttttttgttt gcaagcagca gattacgcgc agatcctttg atcttttcta cggggtctga cgctcagtgg gattttggtc atgagattat caaaaaggat cttcacctag aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
7561 accaatgctt tcatccatag 7621 ttgcctgact tctggcccca 7681 gtgctgcaat gcaataaacc 7741 agccagccgg tccatccagt 7801 ctattaattg ttgcgcaacg 7861 ttgttgccat gcttcattca 7921 gctccggttc aaaaaagcgg 7981 ttagctcctt ttatcactca 8041 tggttatggc tgcttttctg 8101 tgactggtga ccgagttgct 8161 cttgcccggc aaagtgctca 8221 tcattggaaa ttgagatcca 8281 gttcgatgta ttcaccagcg 8341 tttctgggtg agggcgacac 8401 ggaaatgttg tatcagggtt 8461 attgtctcat ataggggttc 8521 cgcgcacatt atcatgacat 8581 taacctataa aaatcataaa 8641 aaatttattt agcggataac 8701 aatttcacac catcaccatc 8761 accatcacgg gtgcccatcc 8821 tggtcgagct gagggcgagg 8881 gcgatgccac aagctgcccg 8941 tgccctggcc gcccgctacc 9001 ccgaccacat tacgtccagg 9061 agcgcaccat gtgaagttcg 9121 agggcgacac gaggacggca 9181 acatcctggg atcatggccg 9241 acaagcagaa gaggacggca 9301 gcgtgcagct cccgtgctgc
aatcagtgag gcacctatct cagcgatctg tctatttcgt ccccgtcgtg tagataacta cgatacggga gggcttacca gataccgcga gacccacgct caccggctcc agatttatca aagggccgag cgcagaagtg gtcctgcaac tttatccgcc ttgccgggaa gctagagtaa gtagttcgcc agttaatagt tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg ccaacgatca aggcgagtta catgatcccc catgttgtgc cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg agcactgcat aattctctta ctgtcatgcc atccgtaaga gtactcaacc aagtcattct gagaatagtg tatgcggcga gtcaatacgg gataataccg cgccacatag cagaacttta acgttcttcg gggcgaaaac tctcaaggat cttaccgctg acccactcgt gcacccaact gatcttcagc atcttttact agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata aatactcata ctcttccttt ttcaatatta ttgaagcatt gagcggatac atatttgaat gtatttagaa aaataaacaa tccccgaaaa gtgccacctg acgtctaaga aaccattatt aaataggcgt atcacgaggc cctttcgtct tcacctcgag gctttgtgag cggataacaa ttataataga ttcaattgtg agaattcatt aaagaggaga aattaactat gagaggatcg atccatggtg agcaagggcg aggagctgtt caccggggtg ggacggcgac gtaaacggcc acaagttcag cgtgtccggc ctacggcaag ctgaccctga agttcatctg caccaccggc caccctcgtg accaccttcg gctacggcgt gcagtgcttc gaagcagcac gacttcttca agtccgccat gcccgaaggc cttcttcaag gacgacggca actacaagac ccgcgccgag cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gcacaagctg gagtacaact acaacagcca caacgtctat gaacggcatc aaggtgaact tcaagatccg ccacaacatc cgccgaccac taccagcaga acacccccat cggcgacggc
9361 tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc 9421 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 9481 agctgtacaa gagcgggcgc c //
Page 1 of 23 Supplementary File S4
- - -
Page 2 of 23
Page 3 of 23
-
Page 4 of 23
- - - - - k1 - - - - - -
Page 5 of 23
k2 AC Glu - - - - - - k3 Gly - - - - - k4 Ser - - - - - - k5 AC
Page 6 of 23
- - - - - - k6 Shikimate Glu - - - - - k7 Phe - - - - - - k8 Shikimate Glu
Page 7 of 23
- - - - - k9 Tyr - - - - - - k10 Shikimate Gln Ser - - - - - k11 Trp
Page 8 of 23
- - - - - - k12 AC - - - - - - k13 Suc Gol - - - - - - k14 Raf
Page 9 of 23
- - - - - - k15 Mel - - - - - - k16 Gal - - - - - - k17 Glc
Page 10 of 23
- - - - - - k18 Suc - - - - - - k19 Fru - - - - - - k20 myoIn AC
Page 11 of 23
- - - - - - k21 myoIn AC - - - - - k22 Asc - - - - - - k23 AC
Page 12 of 23
- - - - - - k24 Pyr Glu - - - - - - k25 Pyr - - - - - k26 Val
Page 13 of 23
- - - - - - k27 Pyr Glu - - - - - k28 Leu - - - - - k29 Ala - -
Page 14 of 23
- - - - k30 Pyr Oxac - - - - - - k31 Cit - - - - - - k32 KGA - - -
Page 15 of 23
- - - k33 Succ - - - - - - k34 Fum - - - - - - k35 Mal - - -
Page 16 of 23
- - - k36 Oxac Glu - - - - - - k37 Asp Gln - - - - - k38 Asn - - -
Page 17 of 23
- - - k39 Asp - - - - - - k39a Asp - - - - - k39b Lys - - - - - -
Page 18 of 23
k39c Asp - - - - - k39d Met - - - - - - k40 Thr Glu - - - - - - k40a
Page 19 of 23
Ile KGA - - - - - - k41 KGA - - - - - - k42 Glu - - - - - k43 Gln
Page 20 of 23
- - - - - - k44 Glu - - - - - k45 Pro - - - - - - k46 Glu - -
Page 21 of 23
- - - - k46a Orn Asp - - - - - - k47 Arg - - - - - - k48 Put - -
Page 22 of 23
- - - - k49 Put - - - - - - k50 Spdine - - - - - k51 Spdine - - - - -
Page 23 of 23
k52 Suc - - - - - - k53 AC
