Quinolone Efffux Pumps Play a Central Role in Emergence of ...

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Jun 18, 2005 - RC2 (efffux pump overexpressed) and RC4 (efffux pump overexpressed and with a Ser79Tyr mutation in parC) were also subsequently studied ...
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Jan. 2006, p. 310–317 0066-4804/06/$08.00⫹0 doi:10.1128/AAC.50.1.310–317.2006 Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Vol. 50, No. 1

Quinolone Efflux Pumps Play a Central Role in Emergence of Fluoroquinolone Resistance in Streptococcus pneumoniae Nelson L. Jumbe,1,2 Arnold Louie,1 Michael H. Miller,2 Weiguo Liu,1,2 Mark R. Deziel,1 Vincent H. Tam,1 Reetu Bachhawat,2 and George L. Drusano1* Ordway Research Institute, Albany, New York,1 and Center for Immunology and Microbial Diseases, Albany Medical College, Albany, New York 2 Received 18 June 2005/Returned for modification 14 August 2005/Accepted 29 September 2005

The preferential use of older antimicrobial agents is, in general, sound public health policy and is meant to maintain susceptibility to newer agents. In the case of fluoroquinolones, however, this strategy is flawed and may actually hasten the spread of Streptococcus pneumoniae strains resistant to newer members of the class. In a mouse thigh infection model, we were unable to isolate clones of pneumococci resistant to the newer fluoroquinolone levofloxacin at 2 ⴛ or 4 ⴛ the baseline MIC. An initial exposure in vivo to the older agent, ciprofloxacin, allowed straightforward selection of clones resistant to levofloxacin in a subsequent experiment. The original ciprofloxacin exposure generated clones without changes in the parC/E and gyrA/B quinolone target sites almost exclusively but did allow overexpression of a reserpine-responsive pump. While this caused only minimal change in the levofloxacin MIC (0.6 mg/liter to 0.8 mg/liter), it allowed a major change in the mutational frequency to resistance for levofloxacin (