Radioimmunoassay of Serum Follicle-stimulating Hormone and ...

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School of Marine and. Atmospheric. Science. University of Miami. Miami,. Florida .... College,. Albany,. NY. 12208. 2Present address: Department of Pathology, ...... bottlenosed dolphins. This represents the first application of RIA tech- niques to.
BIOLOGY

OF

REPRODUCTION

33,

844-85

Radioimmunoassay

of Serum

Luteinizing ALAN

3 (1985)

Follicle-stimulating

Hormone

SCHNEYER,’

ALBERT

Rosenstiel

School

Hormone

in the Bottlenosed

of

CASTRO,2

Marine

Miami,

of

and

DANIEL

Atmospheric

and

University

and

Dolphin ODELL

Science

Miami

Florida

33149

ABSTRACT Commercially

(FSH)

available

radioimmunoassay

(RIA)

kits

for

human

follicle-stimulating

hormone

and

luteinizing hormone (LH) were adapted for quantitation of these hormones in serum from bottlenosed dolphins (Tursiops truncatus). Serum samples from over 160 wild and 70 captive animals were assayed in order to determine basal concentrations of FSH and LH in these animals, as well as to detect possible differences between various groups. Mean FSH and LH levels for all animals were FSH and LU

0.22 ± 0.08 levels than

and 0.37 ± 0.18 captive ones, the

ng/ml, respectively. Although wild animals had higher differences were not statistically significant (P