Research Article Synthesis, Structural, and

Hindawi Publishing Corporation Journal of Chemistry Volume 2013, Article ID 745101, 8 pages http://dx.doi.org/10.1155/2013/745101

Research Article Synthesis, Structural, and Antimicrobial Studies of Some New Coordination Compounds of Palladium(II) with Azomethines Derived from Amino Acids Monika Gupta, Sangeeta Sihag, A. K. Varshney, and S. Varshney Department of Chemistry, University of Rajasthan, Jaipur 302004, India Correspondence should be addressed to S. Varshney; saritavarshney@rediffmail.com Received 27 June 2012; Accepted 17 September 2012 Academic Editor: Andrew S. Brown Copyright © 2013 Monika Gupta et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Some new coordination compounds of palladium(II) have been synthesized by the reaction of palladium(II) acetate with azomethines in a 1 : 2 molar ratio using acetonitrile as a reaction medium. Azomethines used in these studies have been prepared by the condensation of 2-acetyl �uorene and 4-acetyl biphenyl with glycine, alanine, valine, and leucine in methanol. An attempt has been made to probe their bonding and structures on the basis of elemental analyses and IR, 1 H, and 13 C NMR spectral studies. Pd(II) compounds have been found to be more active than their uncomplexed ligands as both of them were screened for antibacterial, antifungal, and insecticidal activities.

1. Introduction Active and well-designed Schiff base ligands are considered as privileged ligands because they are easily prepared by the condensation between aldehyde or ketones and amines and able to stabilize different metals in various oxidation states. e chemistry of Schiff bases has occupied a place of considerable importance because of their well-established biological properties [1]. e Schiff base ligands are coordinated to the investigated metal ion through the azomethine nitrogen either alone or in combination with other electroactive site such as oxygen or sulphur. e N- and O-containing ligands and their complexes have become important due to their wide biological activities [2–5]. It is known that the existence of metal ions bonded to biological active material can enhance their activity [6]. Amino acids and their compounds with different metal ions play an important role in biology, pharmacy, and industry [7–12]. It has been reported that metal complexes of amino acid Schiff bases with transition metals possess anticarcinogenic [13], antimicrobial [14], and antitumor [15] activity.

erefore, the present study was focused on the synthesis, spectral studies of Schiff bases containing, for instances, 2acetyl�uoreneglycine, 2-acetyl�uorenealanine, 2-acetyl�uorenevaline, 2-acetyl�uoreneleucine, 4-acetylbiphenylglycine, 4-acetylbiphenylalanine, 4-acetylbiphenylvaline, and 4-acetylbiphenylleucine moiety and their complexes with Pd(II). e synthesized amino acid derived compounds (L1 H–L8 H) have been exposed to act as bidentate towards divalent metal atom solely through the azomethine nitrogen and carboxylate oxygen forming a stable �ve-membered chelate ring.

2. Experimental 2.1. Analytical Methods and Physical Measurements. All the chemicals used in this work were of AR grade and solvents were dried by a standard method. e reactions were carried out under strictly anhydrous conditions. Nitrogen was estimated by Kjeldahl’s method. IR spectra in the range 4000–250 cm−1 were recorded on a Nicolet Protége 460 FT-IR spectrometer as KBr pellets. A Jeol AL 300 MHz spectrometer was used to obtain the 1 HNMR and 13 C NMR spectra using

2 DMSO-d6 as a solvent. e chemical shis are reported in ppm and trimethylsilane (TMS) is used as a reference compound. Molecular weight determinations were carried out by the Rast camphor method 16. 2.2. Synthesis of Ligands. e azomethine were synthesized by the condensation of 2-acetyl�uorene and 4-acetylbiphenyl with amino acids (glycine, alanine, valine, and leucine) in 1 : 1 molar ratio using methanol as a reaction medium. e solution was re�uxed on a water bath from 5 to 7 h and then allowed to cool at room temperature. e crystalline solids were separated out and puri�ed by recrystallization from the same solvent. e physical properties and analytical data are recorded in Table 1. 2.3. Synthesis of Complex. Azomethine of amino acids (0.416 g–0.642 g 2 mmol) was dissolved in 10 mL of dry acetonitrile in a round bottom �ask. At the same time, palladium(II) acetate (0.225 g, 1 mmol) was dissolved separately in 10 mL of dry acetonitrile. en, the metal solution was added dropwise into the �ask containing the ligand solution. e contents were re�uxed for about 5 hours. e solid derivative obtained was �ltered, washed repeatedly with ethanol, and dried in vacuo. e purity of the compounds was checked by TLC using silica gel-G as an adsorbent. e physical properties and analysis of these complexes are listed in Table 2. 2.4. Biological Activity 2.4.1. Antibacterial Activity. All the synthesized ligands and their corresponding palladium(II) complexes were screened in vitro for their antibacterial activity against two Gramnegative (Escherichia coli and Proteus mirabilis) and two Gram-positive (Bacillus thuringiensis and Staphylococcus aureus) bacterial strains using a paper disc plate method [16, 17]. e nutrient agar medium (peptone, beef extract, NaCl and agar-agar) and 5 mm diameter paper discs of �hatman �lter paper no. 1 were used. e compounds under investigation were dissolved in methanol to give concentrations of 500 and 1,000 ppm. e �lter paper discs were soaked in these solutions, dried, and then placed in petri plates previously seeded with the test organisms. e plates were incubated for 24 h at 28 ± 2∘ C and the inhibition zone around each disc was measured. e antibacterial activity displayed by various compounds is shown in Table 3. 2.4.2. Antifungal Activity. e antifungal activity was evaluated against Aspergillus �avus, Fusarium oxysporum, Aspergillus niger, and Rhizopus phaseoli by the agar plate technique. Solutions of the compounds in different concentrations in DMF were then mixed with the medium. e linear growth [18] of the fungus was recorded by measuring the diameter of colony aer 96 h, and the percentage inhibition was calculated as 100(𝐶𝐶 𝐶 𝐶𝐶𝐶𝐶𝐶𝐶, where 𝐶𝐶 and 𝑇𝑇 are the diameters of the fungus colony in the control and test plates, respectively (Table 4).

Journal of Chemistry 2.4.3. Insecticidal Activity. H. armigera has a long history of insecticide resistance to DDT, pyrethroids, carbonates, organophosphates, and endosulfan. But against endosulfan, it shows less resistance [19]. Hence, the present study is a humble effort in the direction of accomplishing to make new insecticides, which shows less resistance to Helicoverpa armigera like endosulfan. Four synthesized novel azomethine derivatives of amino acids and their complexes have been screened for their insecticidal activity against Helicoverpa armigera, and results are presented in Table 5. e study has been conducted on the second and third instar larval stages of the said insect. Two concentrations, namely, 0.05% and 0.075%, of the test compounds were selected along with the standard check, the endosulfan 35, together with an untreated control. e mortality counts of the insect pests were recorded daily up to ten days.

3. Result and Conclusion Bimolar reaction of palladium(II) acetate with the aforementioned ligand of amino acids in 1 : 2 molar ratio in the presence of acetonitrile can be represented as follows: acetonitrile Pd(CH3 COO) 2 + 2(N OH)

1:2

Pd(N O) 2 + 2CH3 COOH.

e acetic acid formed in these reactions remains soluble in the reaction mixture and solid complexes could be separated by �ltration. e newly synthesized complexes are brightly colored solid and soluble in DMF and DMSO. e molecular weight determined by the Rast Camphor method showed them to be monomer. e molar conductance measurement in DMF at room temperature shows the value in the range 10–15 Ω−1 cm2 mol−1 indicating nonelectrolyte natures of the complexes. 3.1. Spectroscopic Characterization 3.1.1. Signi�cance IR Bands of Starting �aterial� Ligand� and Complex. e IR spectrum of a starting material amino acids shows two bands at 3374 cm−1 and 3308 cm−1 which are characteristics of the NH2 stretching mode while the carbonyl group shows a strong absorption band at 1635 cm−1 which is assigned to the >C=O stretching band. From IR spectrum of Schiff base ligand, new strong and sharp absorption band can be observed at 1618 cm−1 which is assigned for azomethine group >C=N [20] stretching mode. Besides, the absorption bands for NH2 stretching mode and C=O stretching mode have totally disappeared con�rming the formation of azomethine compound. e IR spectrum of palladium(II) complex shows that the absorption band for >C=N stretching mode has been shied from 1618 cm−1 in free ligand to the strong and sharp absorption band at 1595 cm−1 in the complex indicating that the nitrogen atom is involved in bond formation with the metal ion. Besides, the broad O–H band at 3428 cm−1 in the ligand has been disappeared in the complex, suggesting the possible

Journal of Chemistry

3 T 1: Analytical and physical data of the ligands.

Reactant

Elemental analysis Product

MP∘ C

Color

Carbonyl compd.

Amino acids

2-Acetyl�uorene

Glycine

2-Acetyl�uoreneglycine Cream powdery Solid C17 H15 NO2

2-Acetyl�uorene

Alanine

2-Acetyl�uorenealanine C18 H17 NO2

2-Acetyl�uorene

Valine

2-Acetyl�uorenevaline C20 H21 NO2

2-Acetyl�uorene

C found H found N found (Calcd) (Calcd) (Calcd)

Molecular weight found (Calcd)

123

77.09 (76.96)

5.87 (5.67)

5.45 (5.27)

267.67 (265.31)

White Solid

132

77.59 (77.40)

6.19 (6.13)

5.18 (5.01)

280.98 (279.33)

Shining cream Crystal solid

138

77.87 (78.14)

6.57 (6.89)

4.13 (4.56)

305.97 (307.40)

Leucine

2-Acetyl�uoreneleucine White shining Crystal solid C21 H23 NO2

119

78.01 (78.47)

7.09 (7.21)

4.23 (4.36)

320.98 (321.43)

4-Acetylbiphenyl

Glycine

4-Acetylbiphenylglycine Shining cream Powdery solid C16 H15 NO2

114

75.97 (75.87)

6.03 (5.97)

5.68 (5.53)

255.76 (253.30)

4-Acetylbiphenyl

Alanine

4-Acetylbiphenylalanine C17 H17 NO2

White Solid

126

76.58 (76.38)

6.52 (6.41)

5.69 (5.24)

268.98 (267.32)

4-acetylbiphenyl

Valine

4-Acetylbiphenylvaline C19 H21 NO2

Off white Crystal solid

106

78.19 (77.25)

7.20 (7.17)

4.77 (4.74)

296.97 (295.37)

4-Acetylbiphenyl

Leucine

4-Acetylbiphenylleucine Dull off white Shining C20 H23 NO2

99

77.59 (77.64)

7.39 (7.49)

4.38 (4.53)

307.33 (309.41)

T 2: Analytical and physical data of the Pd(II) complexes. Reactant

Elemental analysis Molar ratio

Product

Color

MP∘ C

Pd(OOCCH3 )2 C17 H15 NO2

1:2

Pd(C17 H14 NO2 )2

Reddish brown Solid

186

64.19 (64.31)

4.29 (4.44)

4.36 (4.41)

16.55 (16.76)

634.44 (635.02)


1:2

Pd(C18 H16 NO2 )2

Brown solid Solid

196

65.11 (65.21)

4.59 (4.86)

4.02 (4.22)

15.93 (16.05)

661.66 (663.07)


1:2

Pd(C20 H20 NO2 )2

Dark brown Solid

204

66.56 (66.80)

5.49 (5.61)

3.79 (3.90)

14.56 (14.75)

717.96 (719.18)


1:2

Pd(C21 H22 NO2 )2

Brown green Solid

174

67.19 (67.51)

5.75 (5.93)

3.53 (3.75)

14.01 (14.24)

746.15 (747.24)


1:2

Pd(C16 H14 NO2 )2

Dark brown Solid

165

62.74 (62.90)

4.55 (4.62)

4.42 (4.59)

17.29 (17.42)

609.56 (611.00)


1:2

Pd (C17 H16 NO2 )2

Dark brown Solid

154

63.74 (63.90)

4.95 (5.05)

4.23 (4.38)

16.49 (16.65)

638.45 (639.05)


1:2

Pd(C19 H20 NO2 )2

Brown Solid

145

65.41 (65.66)

5.69 (5.80)

3.94 (4.03)

15.25 (15.31)

694.51 (695.16)


1:2

Pd(C20 H22 NO2 )2

Green Solid

137

66.23 (66.43)

6.04 (6.13)

3.69 (3.87)

14.55 (14.72)

722.45 (723.21)

Metal

Ligand

C found H found N found Pd found (Calcd) (Calcd) (Calcd) (Calcd)

Molecular weight found (Calcd)

4


T 3: Antibacterial screening data of the azomethine derivatives of amino acid and their Pd(II) complexes. Inhibition zone (mm) aer 24 h (concentration in ppm). Compounds C17 H15 NO2 C18 H17 NO2 C16 H15 NO2 C17 H17 NO2 Pd(C17 H14 NO2 )2 Pd(C18 H16 NO2 )2 Pd(C16 H14 NO2 )2 Pd(C17 H16 NO2 )2 Streptomycin

Staphylococcus aureus (+) 500 ppm 1000 ppm 9 10 10 11 8 9 9 10 13 14 12 13 14 15 10 11 16 18

Diameter of inhibition zone (mm) Proteus milamilis (−) Escherichia coli (−) 500 ppm 1000 ppm 500 ppm 1000 ppm 8 10 5 8 10 11 6 8 7 9 6 7 9 12 8 9 8 10 9 10 9 11 10 12 10 13 8 11 10 11 9 10 14 16 15 16

Bacillus thuringiensis (+) 500 ppm 1000 ppm 8 10 9 9 7 11 13 10 10 11 11 13 12 13 13 14 15 17

T 4: Antifungal screening data of azomethine derivatives of amino acid and their Pd(II) complexes. Inhibition (%) aer 96 h concentration 50, 100, and 200 ppm at 25 ± 2∘ C. Compounds C17 H15 NO2 C18 H17 NO2 C16 H15 NO2 C17 H17 NO2 Pd(C17 H14 NO2 )2 Pd(C18 H16 NO2 )2 Pd(C16 H14 NO2 )2 Pd(C17 H16 NO2 )2 Mycostatin

Diameter of inhibition zone (mm) Organism Organism Organism Organism Aspergillus �a�us Fusarium oxysporum Aspergillus niger Rhizopus phaseoli 50 ppm 100 ppm 200 ppm 50 ppm 100 ppm 200 ppm 50 ppm 100 ppm 200 ppm 50 ppm 100 ppm 200 ppm 45 52 61 54 62 75 53 65 73 63 73 86 48 56 65 56 61 71 54 72 91 78 89 79 51 61 63 59 76 79 59 77 86 62 69 89 31 58 57 40 56 63 66 73 96 37 69 76 30 41 61 60 63 74 75 81 84 65 76 83 48 55 66 56 72 73 56 73 86 60 66 83 45 51 72 67 71 84 74 83 86 62 80 63 47 52 74 70 73 85 76 85 88 65 75 76 69 86 98 72 82 96 70 91 100 71 86 100

deprotonation on complexation and the formation of Pd–O bond. e appearance of new and strong medium intensity bands in the spectra of complexes in the region 360–365 cm−1 and may be attributed to 𝑣𝑣(Pd–N) [21] 400–410 cm−1 due to (Pd–O) [22], respectively. 3.2. 1 H NMR Spectra. e coordination of the metal to nitrogen and oxygen atoms is further supported by comparison of the 1 H NMR spectral data of the ligand and its complexes. In the proton magnetic resonance spectra of the ligands, a sharp signal at 𝛿𝛿1.80 ppm is observed due to –C(CH3 )=N–. is moves down�eld (𝛿𝛿1.98 ppm) in the complexes in comparison to its original position in the ligands due to coordination of azomethine nitrogen to the metal atom [23]. e ligands show the OH proton signal at 𝛿𝛿11.10 ppm. However, in the complexes, these signals disappear showing the chelation through the carboxylic group. e ligand shows a complex multiplet signal in the region at 𝛿𝛿7.28–8.39 ppm for the aromatic protons and it remain almost at the same position in spectra of the metal complexes. e results are given in Table 6.

3.3. 13 C NMR Spectra. e 13 C NMR spectral data for ligands and its corresponding Pd complexes in dry DMSO have been recorded in Table 7 and Scheme 1. e 13 C NMR spectrum showed the displacement of azomethine carbon (>C=N–) from 𝛿𝛿175.13 in the noncoordinated ligand to the down�eld 𝛿𝛿219.15 in the complex due to the coordination of azomethine nitrogen atom to the palladium metal. erefore, a four-coordinate square planar geometry may be proposed for the resulting Pd(II) complexes. us, on the basis of the above discussion, it is clear that the ligand, by coordinating to Pd atom through the azomethine nitrogen, behaves as a bidentate ligand. 3.4. Antimicrobial Results. Antimicrobial tests of the ligands and their complexes on two Gram-negative (Escherichia coli and Proteus mirabilis) and two Gram-positive (Bacillus thuringiensis and Staphylococcus aureus) bacterial and selected fungi Aspergillus �a�us� Fusarium oxysporum� Aspergillus niger and Rhizopus phaseoli were carried out. ese results clearly indicate that the metal complexes are more active than the starting material and this is in


5

5

5 3 C

2 CH2

N

1 COOH

2 CH2

N

CH3 4 5 L H

1 COOH

6 CH3

3 C

N

CH 2

CH3 4 6 L H

O

CH3

C O

N

C

O

O

Pd(L 1)2 O

CH3 Pd

N C O

N

CH3

Pd(L 2)2 O O

C N

O

N C

H3C

CH3

Pd(L 5)2

C

O

CH3

C

O

C

Pd C

H3C

CH3

CH3

C

O

C

N

Pd

N

1 COOH

O

CH3

C

O

C

1 COOH

CH 2

N

CH3 4 L2H

CH3 4 L1H 3 C

6 CH3

3 C

Pd C O

CH3

C N

O

C CH3

Pd(L 6)2

S 1

T 5: Percentage mortality of Helicoverpa armigera pest aer 1, 3, 7, and 10 days. Total percent mortality (concentration). Serial Number

Percentage mortality

Treatment

Day 1

Day 3

a 25.0 (41.4)

b 37.5 (62.1)

a 50.5 (71.4)

b 75.0 (88.4)

a 137.5 (127.7)

Day 5 b 150.0 (135.0)

Day 10 a b 175.0 200.0 (149.5) (166.5)

1

Compound 1

2

Compound 2

25.0 (41.4)

37.5 (62.1)

75.0 (88.5)

87.5 (97.8)

162.5 (142.8)

187.5 (157.2)

212.5 (172.2)

225.0 (181.5)

3

Compound 3

50.0 (71.4)

87.5 (97.7)

100.0 (105.5)

137.5 (127.8)

200.0 (166.5)

225.0 (181.5)

237.5 (189.3)

262.5 (219.3)

4

Compound 4

62.5 (80.7)

100.0 (105.5)

100.0 (105.5)

137.5 (127.8)

212.5 (172.2)

225.0 (181.5)

250.0 (198.6)

275.0 (228.6)

Endosulfan

87.5 (97.8) 0.0 (0.0)

Control

a = 0.05% concentration and b = 0.075% concentration.

137.5 (127.8) 0.0 (0.0)

212.5 (172.2) 0.0 (0.0)

275.0 (228.6) 0.0 (0.0)

6

Journal of Chemistry T 6: 1 H NMR spectral data of the ligands and their corresponding Pd(II) complexes.

Compound

COOH 11.52 (s) — 11.68 (s) — 11.54 (s) — 11.48 (s) — 11.56 (s) — 11.65 (s) — 11.59 (s) — —

L1 H Pd(L1 )2 L2 H Pd(L2 )2 L3 H Pd(L3 )2 L4 H Pd(L4 )2 L5 H Pd(L5 )2 L6 H Pd(L6 )2 L7 H Pd(L7 )2 Pd(L8 )2

N–CH–C — — 4.48 (q) 4.68 (q) 4.10 (d) 4.16 (d) 4.49 (t) 4.54 (t) — — 4.53 (q) 4.75 (q) 4.12 (d) 4.16 (d) 4.55 (t)

Chemical shi in (𝛿𝛿 ppm) –C(CH3 )N– –CH–CH3 1.83 (s) — 1.95 (s) — 1.90 (s) 1.48 (d) 1.98 (s) 1.50 (d) 1.94 (s) 1.07 (d) 1.99 (s) 1.10 (d) 1.87 (s) 1.07 (d) 1.94 (s) 1.09 (d) 1.85 (s) — 1.93 (s) — 1.92 (s) 1.47 (d) 1.99 (s) 1.51 (d) 1.91 (s) 1.07 (d) 1.97 (s) 1.10 (d) 1.96 (s) 1.10 (d)

–CH2 – 4.22 (s) 4.26 (s) — — — — 1.56 (q) 1.57 (q) 4.24 (s) 4.26 (s) — — — — 1.58 (q)

H3 C–CH–CH3 — — — — 2.39 (m) 2.41 (m) 0.98 (m) 1.01 (m) — — — — 2.38 (m) 2.42 (m) 1.03 (m)

Aromatic protons (6.98–8.20) (m) (6.92–8.12) (m) (6.88–8.15) (m) (6.82–8.10) (m) (6.95–8.15) (m) (6.85–8.10) (m) (6.92–8.12) (m) (6.84–8.12) (m) (6.98–8.15) (m) (6.95–8.15) (m) (6.95–8.25) (m) (6.85–8.15) (m) (6.95–8.20) (m) (6.80–8.05) (m) (6.80–8.10) (m)

T 7: 13 C NMR spectral data of the ligands and their corresponding Pd(II) complexes. Chemical shi in (𝛿𝛿 ppm)

Compound

C1 175.9 166.3 175.7 168.3 174.3 169.7 177.2 166.7

L1 H Pd(L1 )2 L2 H Pd(L2 )2 L5 H Pd(L5 )2 L6 H Pd(L6 )2

C2 63.6 62.9 64.5 65.9 65.2 66.1 71.3 71.5

C3 165.2 154.8 163.1 155.7 164.7 156.3 161.7 153.8

C4 17.1 17.2 17.3 17.2 17.1 17.0 17.4 17.5

C5 34.4 34.7 34.8 34.9 — — — —

C6 — — 18.8 19.3 — — 18.9 18.5

Aromatic carbon 136.9, 128.4, 119.1, 138.8 142.2, 129.2, 136.9, 128.4, 119.1, 164.2, 122.2, 129.8 137.6, 128.6, 119.3, 138.8 142.2, 129.2, 136.9, 128.4, 119.1, 164.2, 122.2, 129.8 137.2, 128.5, 119.5, 138.9 142.8, 129.2, 136.9, 128.4, 119.1, 164.2, 122.2, 129.8 137.4, 128.7, 119.7, 139.1 142.8, 129.2, 136.9, 128.4, 119.1, 164.2, 122.2, 129.8 136.2 129.4, 127.1, 138.8 138.2, 127.2, 136.9, 127.4, 129.1, 127.2, 129.2, 127.8 137.5 129.9, 127.8, 139.2 138.6, 127.2, 136.9, 127.4, 129.1, 127.2, 129.2, 127.8 136.2 129.7, 127.6, 139.3 138.4, 127.2, 136.9, 127.4, 129.1, 127.2, 129.2, 127.8 137.8 129.9, 127.9, 139.6 138.8, 127.2, 136.9, 127.4, 129.1, 127.2, 129.2, 127.8

ion, mainly, because of the partial sharing of its positive charge with the donor groups and possibly the 𝜋𝜋-electron delocalization within the whole chelate ring [26–28]. is process of chelation thus increases the lipophilic nature of the central metal atom, which in turn favours its permeation through the lipid layer of the membrane [29–31].

Total mortality (%)

250 200 150 100 50 0

a

b

Compound1 Compound2 Compound3

a b a b Treatments (concentration)

a

b

Compound4 Endosulfan

F 1: Total percentage of mortality of third instar H. armigera pest aer 1, 3, 7, and 10 days.

accordance with the fact that chelation increases the activity. e chelation reduces the polarity [24, 25] of the metal

3.5. Antiinsecticidal Result. To study the structure-activity relationship here we, have selected four azomethine amino acids and their metal complexes’ derivatives where the aromatic ring was the same; that is, �uorene ring. e carbonyl moiety was also the same; the only variation was in the alkyl substituted group. is study has suggested that an increase in the bulkiness of alkyl substituent and presence of the C=N moiety enhanced the bioactivity as evidenced from the experimental details of the study (Table 5, Figure 1). Compound 3 and compound 4 at concentrations 0.05% and 0.075% showed the best insecticidal activity, which was found to be superior to that of a standard insecticide endosulfan. us, the observed enhancement of activity of those complexes that were found to be more active than ligand must be due to a combination effect associate with the

Journal of Chemistry derivatization and complexation of the ligand and presence of the side group of the amino acid.

4. Conclusion All the synthesized compounds show higher activity than the ligands but slightly lower than the standard drug. e compounds showed good antibacterial activity against S. aureus and B. thuringiensis than E. coli and P. mirabilis. e ligands and their complexes exhibit more signi�cant effect on R. phaseoli and A. niger species than on F. oxysporum and A. �avus. e compounds showed toxicity at 200 ppm against all species of fungi. e activity decreased on dilution.

Acknowledgments e authors are thankful to the Head of the Department of Chemistry, University of Rajasthan, Jaipur, for providing laboratory facilities and constant encouragement. M. Gupta and S. Sihag are thankful to CSIR, New Delhi, for providing �nancial assistance.

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