ResEnzymeData.qxd (Page 1) - Fisher Scientific

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BP3314-1. Ava II. Anabaena variabilis. 1–10 units/µL. 100 units. BP3316-1. Bal I ... Csp I. Clostridium species. 10 units/µL. 100 units. BP3346-1. Csp45 I.
Restriction Enzymes Quality assurance— tested for activity and functional purity Fisher Ordering Information Activity unit definition: One unit of restriction enzyme is defined as the amount of enzyme required to produce a complete digest of one microgram of substrate DNA in 60 minutes at the appropriate assay temperature in a 50µL reaction volume. Restriction endonuclease BAMH I bound to DNA

➔

Alu I

➔

5'… A G C T …3' 3'… T C G A …5'

BAMH I ➔ ➔

5'… G G A T C C …3' 3'… C C T A G G …5' ➔

EcoR I

➔

5'… G A A T T C …3' 3'… C T T A A G …5'

Hae III ➔ ➔

5'… G G C C …3' 3'… C C G G …5'

Description

Source

Concentration

Quantity

Cat. No.

Aat II Acc I Acc III Acc65 I Alu I Alw44 I Apa I Ava I Ava II

Acetobacter aceti Acinetobactor calcoaceticus Acinetobactor calcoaceticus Acinetobactor calcoaceticus Arthrobacter luteus Acinetobacter lwoffi RFL 44 Acetobacter pasteurianus Anabaena variabilis Anabaena variabilis

3–5 units/µL 3–10 units/µL 10 units/µL 10 units/µL 10 units/µL 10 units/µL 8–12 units/µL 8–12 units/µL 1–10 units/µL

50 units 100 units 200 units 1500 units 500 units 1000 units 5000 units 200 units 100 units

BP3300-1 BP3302-1 BP3304-1 BP3306-1 BP3308-1 BP3310-1 BP3312-1 BP3314-1 BP3316-1

Bal I BamH I

Brevibacterium albidum Bacillus amyloliquefaciens H

Ban II Bcl I Bgl I Bgl II Bsp1286 I BsrBR I BssH II Bst98 I BstE ll BstO I BstX I

Bacillus Bacillus Bacillus Bacillus Bacillus Bacillus Bacillus Bacillus Bacillus Bacillus Bacillus

2–10 units/µL 10 units/µL 40–80 units/µL 8–12 units/µL 40–80 units/µL 10 units/µL 10 units/µL 10 units/µL 10 units/µL 10 units/µL 8–12 units/µL 10 units/µL 10 units/µL 8–12 units/µL

50 units 2500 units 50,000 units (HC) 1000 units 5000 units (HC) 1000 units 500 units 500 units 2000 units 100 units 500 units 2000 units 2000 units 250 units

BP3318-1 BP3320-1 BP3320-7 BP3322-1 BP3324-5 BP3325-1 BP3326-1 BP3328-1 BP3330-1 BP3332-1 BP3334-1 BP3336-1 BP3338-1 BP3340-1

Cfo I Cla I Csp I Csp45 I

Clostridium formicoaceticum Caryophanon latum L Clostridium species Clostridium sporogenes

10 10 10 10

units/µL units/µL units/µL units/µL

3000 units 500 units 100 units 2500 units

BP3342-1 BP3344-1 BP3346-1 BP3348-1

Dde I Dpn I Dra I

Desulfovibrio desulfuricans Recombinant E. coli strain Deinococcus radiophilus

8–12 units/µL 10 units/µL 10 units/µL

200 units 200 units 2000 units

BP3350-1 BP3352-1 BP3354-1

EcoR V Eco47 III Eco52 I EcoR I

Escherichia Escherichia Escherichia Escherichia

10 units/µL 2–5 units/µL 1–5 units/µL 8–12 units/µL 40–80 units/µL

2000 units 50 units 50 units 5000 units 50,000 units (HC)

BP3356-1 BP3358-1 BP3360-1 BP3362-1 BP3362-7

aneurinolyticus caldolyticus globigii globigii sphaericas (IAM 1286) stearothermophilus stearothermophilus H3 stearothermophilus stearothermophilus stearothermophilus stearothermophilus XI

coli coli coli coli

J62 pLG74 RFL 47 RFL 52 RY 13

Fok I

Flavobacterium okeanokoites

2–10 units/µL

100 units

BP3364-1

Hae II Hae III Hinc II Hind III

Recombinant E. coli strain Haemophilus aegyptius Haemophilus influenzae Rc Haemophilus influenzae Rd

Hinf I Hpa I Hpa II

Haemophilus influenzae Rf Haemophilus parainfluenzae Haemophilus parainfluenzae

10 units/µL 10 units/µL 10 units/µL 10 units/µL 40–80 units/µL 10 units/µL 3–10 units/µL 10 units/µL

1000 units 2500 units 200 units 5000 units 50,000 units (HC) 1000 units 100 units 1000 units

BP3366-1 BP3368-1 BP3370-1 BP3372-1 BP3372-7 BP3374-1 BP3376-1 BP3378-1

Fisher Ordering Information Continued Description

Source

Concentration

Quantity

Cat. No.

Kpn I

Klebsiella pneumoniae OK8

8–12 units/µL 40–80 units/µL

2500 units 12,500 units (HC)

BP3380-1 BP3380-7

Mlu I Msp I

Micrococcus luteus Moraxella species

10 units/µL 10 units/µL

1000 units 2000 units

BP3382-1 BP3384-1

Nae I Nar I Nco I Nde I Nde II Nhe I Not I Nru I Nsi I

Nocardia aerocolonigenes Nocardia argentinensis Nocardia corallina Neisseria denitrificans Neisseria denitrificans Neisseria mucosa heidelbergensis Nocardia otitidis-caviarum Nocardia rubra ATCC 15906 Neisseria sicca

4 units/µL 10 units/µL 10 units/µL 10 units/µL 10 units/µL 10 units/µL 10 units/µL 10 units/µL 10 units/µL

250 200 200 500 200 250 200 200 250

BP3386-1 BP3388-1 BP3390-1 BP3392-1 BP3394-1 BP3396-1 BP3398-1 BP3400-1 BP3402-1

Pst I Pvu I Pvu II

Recombinant E. coli strain Proteus vulgaris Proteus vulgaris

10 units/µL 2–10 units/µL 8–12 units/µL

3000 units 100 units 1000 units

BP3404-1 BP3406-1 BP3408-1

Rsa I

Rhodopseudomonas sphaeroides

10 units/µL 40–80 units/µL

1000 units 5000 units (HC)

BP3410-1 BP3410-5

Sac I Sac II Sal I Sau3A I Sau96 I Sca I

Streptomyces achromogenes Streptomyces achromogenes Streptomyces albus G Staphylococcus aureus 3A Staphylococcus aureus PS96 Streptomyces caespitosus

Sfi I

Streptomyces fimbriatus

Sma I SnaB I Spe I Sph I Ssp I

Serratia marcescens Sphaerotilus natans ATCC 139280 Sphaerotilus natans ATCC 13923 Streptomyces phaeochromogenes Sphaerotilus natans

Stu I Sty I

Streptomyces tubercidicus Recombinant E. coli strain

10 units/µL 10 units/µL 10 units/µL 3–10 units/µL 10 units/µL 8–12 units/µL 40–80 units/µL 10 units/µL 40–80 units/µL 8–12 units/µL 2–10 units/µL 10 units/µL 10 units/µL 10 units/µL 40–80 units/µL 10 units/µL 10 units/µL

1000 units 500 units 2000 units 100 units 300 units 1000 units 5000 units (HC) 250 units 1250 units (HC) 1000 units 100 units 200 units 200 units 500 units 2500 units (HC) 400 units 2000 units

BP3412-1 BP3414-1 BP3416-1 BP3418-1 BP3420-1 BP3422-1 BP3422-5 BP3424-1 BP3424-5 BP3426-1 BP3428-1 BP3430-1 BP3432-1 BP3434-1 BP3434-5 BP3436-1 BP3438-1

Taq I

Thermus aquaticus YT1

Tru9 I Tth111 I

Thermus ruber 9 Thermus thermophilus 111

10 units/µL 40–80 units/µL 8–12 units/µL 8–12 units/µL

1000 units 5000 units (HC) 200 units 500 units

BP3440-1 BP3440-7 BP3442-1 BP3444-1

Vsp I

Vibrio species

8–12 units/µL

500 units

BP3446-1

Xba I

Xanthomonas badrii

Xho I Xho II Xmn I

Xanthomonas holcicola Xanthomonas holcicola Xanthomonas manihotis 7AS1

8–12 units/µL 40–80 units/µL 10 units/µL 5–10 units/µL 10 units/µL

2000 units 10,000 units (HC) 3000 units 100 units 500 units

BP3448-1 BP3448-7 BP3450-1 BP3452-1 BP3454-1

units units units units units units units units units

Supplied in other convenient packaging sizes.

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