Meloidogyne cf. hapla 8 Meloidogyne hapla 46 .... Rolylenchulus reniformis genotype 8 consensus .... 100 Trophurus ussu riensis 1 Troph urus sculplus 1.
1
Disparate gain ad loss patterns
0.79
0.55
1
0.81
1
0.52
0.67
0.81
1
0.63
0.97
0.89
1
1
1
0.87
1
0.92
1
1
Trophurus ussuriensis 1 Trophurus ussuriensis 2
Tylenchorhynchus claytoni 1G Tylenchorhynchus claytoni 2G
1
1
0.92
0.96
1
0.85
1
1
1
0.69
1
1
1
0.57
0.97
Pratylenchus thornei 1 Pratylenchus thornei 3 Pratylenchus thornei 4
0.88
0.62
0.83
Pratylenchus pratensis 4 Pratylenchus pratensis 5
1
1
1
Hirschmanniella n. sp. 1 Hirschmanniella loofi 1
1
0.85
1
1
1
Pratylenchus oleae 1G Pratylenchus oleae 2G
0.98
1
Meloidogyne coffeicola 1G
0.69
1
0.86
1
0.84
0.74
0.96
0.54
1
1
1
0.9
0.77
0.78
1 0.78
1
1
0.75
Radopholus similis 3G Radopholus bridgei 1G Radopholus sp. 2
Meloidogyne hapla 11
Pratylenchidae - Radopholinae
Meloidogyne incognita 12G Meloidogyne incognita 17G Meloidogyne incognita 13G Meloidogyne incognita 18G Meloidogyne incognita 14G Meloidogyne incognita 15G Meloidogyne arenaria 1 Meloidogyne arenaria 4G Meloidogyne arenaria 11G Meloidogyne arenaria 1G Meloidogyne arenaria 5G Meloidogyne arenaria 13G Meloidogyne arenaria 2G Meloidogyne arenaria 6G Meloidogyne arenaria 14G Meloidogyne arenaria 3G Meloidogyne arenaria 8G
Meloidogyne morocciensis 1G Meloidogyne floridensis 1G Meloidogyne lopezi 1G Meloidogyne cruciani 1G
Meloidogyne sp. 2G Meloidogyne sp. 3G Meloidogyne sp. 8 Meloidogyne sp. 9 Meloidogyne sp. 10
Sedentary endoparasites - 1.
Meloidogynidae
Meloidogyne kralli 1
Meloidogyne enterolobii 13G Meloidogyne enterolobii 17G Meloidogyne enterolobii 21G Meloidogyne enterolobii 25G Meloidogyne enterolobii 29G Meloidogyne enterolobii 14G Meloidogyne enterolobii 18G Meloidogyne enterolobii 22G Meloidogyne enterolobii 26G Meloidogyne mayaguensis 1G Meloidogyne enterolobii 15G Meloidogyne enterolobii 19G Meloidogyne enterolobii 23G Meloidogyne enterolobii 27G Meloidogyne enterolobii 20G Meloidogyne enterolobii 24G Meloidogyne enterolobii 28G Meloidogyne enterolobii 16G Meloidogyne chitwoodi 1 Meloidogyne chitwoodi 5 Meloidogyne chitwoodi9 9 Meloidogyne chitwoodi 1G Meloidogyne chitwoodi 5G Meloidogyne chitwoodi 9G Meloidogyne chitwoodi 13G Meloidogyne chitwoodi 2 Meloidogyne chitwoodi 6 Meloidogyne chitwoodi10 10 Meloidogyne chitwoodi 2G Meloidogyne chitwoodi 6G Meloidogyne chitwoodi 10G Meloidogyne chitwoodi 14G Meloidogyne chitwoodi 3 Meloidogyne chitwoodi 7 Meloidogyne chitwoodi 11 Meloidogyne chitwoodi 3G Meloidogyne chitwoodi 7G Meloidogyne chitwoodi 11G Meloidogyne chitwoodi 15G Meloidogyne chitwoodi 4 Meloidogyne chitwoodi8 8 Meloidogyne chitwoodi 12 Meloidogyne chitwoodi 4G Meloidogyne chitwoodi 8G Meloidogyne chitwoodi 12G Meloidogyne chitwoodi 16G Meloidogyne fallax 1 Meloidogyne fallax 10 Meloidogyne fallax 14 Meloidogyne fallax 2G Meloidogyne fallax 6G Meloidogyne fallax 10G Meloidogyne fallax 14G Meloidogyne fallax 6 Meloidogyne fallax 11 Meloidogyne fallax 15 Meloidogyne fallax 3G Meloidogyne fallax 7G Meloidogyne fallax 11G Meloidogyne fallax 7 Meloidogyne fallax 12 Meloidogyne fallax 16 Meloidogyne fallax 4G Meloidogyne fallax 8G Meloidogyne fallax 12G Meloidogyne fallax 9 Meloidogyne fallax 13 Meloidogyne fallax 1G Meloidogyne fallax 5G Meloidogyne fallax 9G Meloidogyne fallax 13G
Meloidogyne hapla 3G Meloidogyne hapla 4G Meloidogyne hapla 5G Meloidogyne hapla 6G
1 Meloidogyne minor 1 Meloidogyne minor 5G Meloidogyne minor 1G Meloidogyne minor 6G 0.99 Meloidogyne minor 2 Meloidogyne minor 7G Meloidogyne minor 4G Meloidogyne minor 8G Meloidogyne graminicola 2G Meloidogyne graminicola 6G 0.63 Meloidogyne graminicola 3G Meloidogyne graminicola 7G Meloidogyne graminicola 4G Meloidogyne graminicola 8G 0.55 Meloidogyne graminicola 5G Meloidogyne oryzae G Meloidogyne graminicola 1G Meloidogyne naasi 1 Meloidogyne naasi 2G Meloidogyne naasi 6G 0.53 0.73 Meloidogyne naasi 2 Meloidogyne naasi 7G Meloidogyne naasi 3G Meloidogyne naasi 3 Meloidogyne naasi 4G Meloidogyne naasi 8G Meloidogyne naasi 4 Meloidogyne naasi 5G Meloidogyne naasi 9G
Meloidogyne exigua 1G Meloidogyne exigua 3G
Meloidogyne hapla 4 Meloidogyne cf. hapla 8 Meloidogyne hapla 9 Meloidogyne hapla 10
Meloidogyne spartinae 1G Meloidogyne graminis 1G Meloidogyne graminis 5G Meloidogyne graminis 15G 0.88 Meloidogyne graminis 2G Meloidogyne graminis 6G Meloidogyne graminis 3G Meloidogyne graminis 7G Meloidogyne graminis 4G Meloidogyne graminis 14G 0.64 Meloidogyne graminis 12G Meloidogyne marylandi 1G Meloidogyne marylandi 2G Meloidogyne marylandi 3G Meloidogyne graminis 9G Meloidogyne marylandi 4G Meloidogyne graminis 10G Meloidogyne marylandi 5G Meloidogyne graminis 11G Meloidogyne graminis 13G Meloidogyne graminis 8G
Sedentary semi-endoparasites - 3.
Rotylenchulidae
Migratory endoparasites Ectoparasites
Radopholus similis 3 Radopholus similis 1G Radopholus similis 2G
Ectoparasites / Semi-endoparasites
Helicotylenchus multicinctus 2G Helicotylenchus multicinctus 3G
Helicotylenchus dihystera 9G Helicotylenchus dihystera 10G Helicotylenchus sp. 6 Helicotylenchus rotundicauda 1G
Rotylenchulus borealis 1 Rotylenchulus borealis 2 Rotylenchulus reniformis genotype 1 consensus Rotylenchulus reniformis genotype 6 consensus Rotylenchulus reniformis genotype 7 consensus Rotylenchulus reniformis genotype 9 consensus
Dolichodoridae
1
1
Meloidogyne ethiopica 1G Meloidogyne ethiopica 5G Meloidogyne sp. 1G Meloidogyne ethiopica 2G Meloidogyne ethiopica 6G Meloidogyne ethiopica 3G Meloidogyne ethiopica 7G Meloidogyne ethiopica 4G Meloidogyne ethiopica 8G Meloidogyne incognita 1 Meloidogyne incognita 2G Meloidogyne incognita 7G Meloidogyne incognita 2 Meloidogyne incognita 3G Meloidogyne incognita 9G Meloidogyne incognita 3 Meloidogyne incognita 4G Meloidogyne incognita 10G 0.6 Meloidogyne incognita 1G Meloidogyne incognita 5G Meloidogyne incognita 11G 0.53 Meloidogyne javanica 1 Meloidogyne javanica 6G Meloidogyne javanica 10G Meloidogyne javanica 11G Meloidogyne javanica 1G Meloidogyne javanica 7G 0.51 Meloidogyne javanica 12G Meloidogyne javanica 2G Meloidogyne javanica 8G Meloidogyne javanica 3G Meloidogyne javanica 9G Meloidogyne incognita 8G 0.59 Meloidogyne lopezi 2G Meloidogyne arabicida 2G Meloidogyne izalcoensis 1G 0.52 Meloidogyne lopezi 5G Meloidogyne arabicida 3G Meloidogyne izalcoensis 2G Meloidogyne lopezi 3G Meloidogyne sp. 4G Meloidogyne arabicida 4G 0.51 Meloidogyne lopezi 4G Meloidogyne paranaensis 1G 0.99 Meloidogyne arabicida 1G 0.91 Meloidogyne konaensis 1G Meloidogyne arenaria 5 Meloidogyne incognita 19G Meloidogyne hispanica 1G Meloidogyne javanica 4G Meloidogyne incognita 6G Meloidogyne javanica 5G Meloidogyne javanica 2 Meloidogyne incognita 4 Meloidogyne enterolobii 1G Meloidogyne enterolobii 5G Meloidogyne enterolobii 9G Meloidogyne enterolobii 2G Meloidogyne enterolobii 6G Meloidogyne enterolobii 10G Meloidogyne enterolobii 3G Meloidogyne enterolobii 7G Meloidogyne enterolobii 11G Meloidogyne enterolobii 4G Meloidogyne enterolobii 8G Meloidogyne enterolobii 12G
1
1
0.61 Meloidogyne exigua 2G Meloidogyne duytsi 1G 1 Meloidogyne duytsi 1 Meloidogyne duytsi 2 1 Meloidogyne microtyla 1G 1 1 Meloidogyne hapla 2G Meloidogyne hapla 5 Meloidogyne hapla 6 Meloidogyne hapla 12
0.69
Dolichodorus sp. 1G Dolichodorus sp. 2G Dolichodorus heterocephalus 1G
1
Helicotylenchus dihystera 5G Helicotylenchus dihystera 6G Helicotylenchus dihystera 7G Helicotylenchus dihystera 8G
Helicotylenchus pseudorobustus genotype 1 consensus Helicotylenchus certus 2 Helicotylenchus certus 1 Helicotylenchus dihystera 1G Helicotylenchus dihystera 2G Helicotylenchus dihystera 3G Helicotylenchus dihystera 4G
1
Helicotylenchus pseudorobustus genotype 2 consensus Helicotylenchus sp. 5
1 Helicotylenchus multicinctus 1 Helicotylenchus multicinctus 1G Helicotylenchus crenacauda 1G 1 Helicotylenchus indicus 1 Helicotylenchus indicus 3 Helicotylenchus indicus 2 Helicotylenchus indicus 4 Helicotylenchus paraplatyurus 1G Helicotylenchus paraplatyurus 2G Helicotylenchus digitiformis 1 Helicotylenchus digitiformis 2 Helicotylenchus digitiformis 4 Helicotylenchus digitiformis 6
Meloidogyne artiellia 1 Meloidogyne artiellia 2 Meloidogyne artiellia 1G Meloidogyne artiellia 2G
Meloidogyne ichinohei 4 Meloidogyne ichinohei 1 Meloidogyne ichinohei 2 Meloidogyne ichinohei 1G Meloidogyne ichinohei 3 Meloidogyne sp. 1 1 Meloidogyne sp. 2 Meloidogyne sp. 4
Meloidogyne mali 2 Meloidogyne mali 1G
Meloidogyne ardenensis 1 1 Meloidogyne ardenensis 2 Meloidogyne maritima 1 Meloidogyne maritima 2 Meloidogyne maritima 1G 1 Meloidogyne ulmi 1 Meloidogyne ulmi 4 Meloidogyne mali 1 1
Pratylenchus convallariae 1 Pratylenchus convallariae 2 Pratylenchus fallax 1
0.99
0.55
Pratylenchus penetrans 1 Pratylenchus penetrans 2 Pratylenchus penetrans 1G Pratylenchus cf. penetrans 2G
Pratylenchus neglectus 5G Pratylenchus neglectus 6G
1
Pratylenchidae - Pratylenchinae, Hirschmanniellinae Pratylenchus neglectus 1G Pratylenchus neglectus 2G Pratylenchus neglectus 3G Pratylenchus neglectus 4G
Heterodera glycines 5G Heterodera glycines 6G Heterodera glycines 7G
Heteroderidae
Globodera artemisiae 2 Heterodera avenae 1 Heterodera filipjevi 1 Heterodera filipjevi 3G Heterodera avenae 2 Heterodera filipjevi 2 Heterodera filipjevi 4G Heterodera avenae 3 Heterodera filipjevi 1G Heterodera mani 1 Heterodera avenae 4 Heterodera filipjevi 2G Heterodera sp. 1 Heterodera schachtii 5 Heterodera betae 2 Heterodera trifolii 2 Heterodera schachtii 6 Heterodera hordecalis 1 Heterodera trifolii 4 Heterodera schachtii 1G Heterodera trifolii 5 Heterodera trifolii 1 Heterodera betae 1 Heterodera glycines 2G Heterodera glycines 3G Heterodera glycines 4G
Heterodera schachtii 1 Heterodera schachtii 2 Heterodera schachtii 3 Heterodera schachtii 4
Helicotylenchus sp. 3 Helicotylenchus sp. 4
0.64
Helicotylenchus cf. digitiformis 7 Helicotylenchus cf. digitiformis 8 Helicotylenchus sp. 1
Migratory endoparasites
1
Helicotylenchus minzi 5 Helicotylenchus sp. 2 Helicotylenchus platyurus 1 Helicotylenchus platyurus 2 Helicotylenchus varicaudatus 1G 0.62
Helicotylenchus pseudodigonicus 4 Helicotylenchus pseudodigonicus 5 Helicotylenchus scoticus 1G
Helicotylenchus canadensis 1 Helicotylenchus canadensis 2 Helicotylenchus digonicus 1
Helicotylenchus sp. 1G Helicotylenchus sp. 2G Helicotylenchus sp. 3G
Helicotylenchus digonicus 1G Helicotylenchus minzi 1 Helicotylenchus minzi 2 Helicotylenchus minzi 3 Helicotylenchus minzi 4
Helicotylenchus minzi 3G Helicotylenchus minzi 4G
Helicotylenchus minzi 1G Helicotylenchus minzi 2G
0.96
1
Sedentary endoparasites - 3.
Globodera sp. 7 Globodera sp. 8
Globodera tabacum 3 Globodera tabacum 4
Heterodera elachista 1G 1 Heterodera elachista 2G Punctodera punctata 1 Afenestrata koreana 1G
0.88
Globodera sp. 3 Globodera sp. 4 Globodera sp. 5 Globodera sp. 6
Cactodera sp. 1G Cactodera sp. 2G Cactodera sp. 3G Cryphodera brinkmani 1G Cryphodera sinensis 1G 1
Sedentary endoparasites - 2.
Pratylenchus goodeyi 1G
Pratylenchus neglectus 1 Pratylenchus neglectus 2 Pratylenchus neglectus 3 Pratylenchus neglectus 4
Pratylenchus araucensis 1G
Pratylenchus vulnus 3 Pratylenchus vulnus 4
0.74
0.95
Helicotylenchus digitiformis 3
Hoplolaimidae
Rotylenchulus reniformis genotype 8 consensus 1 Rotylenchulus reniformis genotype 2 consensus Rotylenchulus reniformis genotype 4 consensus Rotylenchulus reniformis genotype 3 consensus Rotylenchulus reniformis genotype 5 consensus Rotylenchus sp. 1 1 Scutellonema bradys 1G Scutellonema bradys 2G 1 Helicotylenchus digonicus 6G Helicotylenchus digonicus 7G Helicotylenchus digonicus 8G
1
Globodera rostochiensis 4G Globodera rostochiensis 5G Globodera rostochiensis 6G Globodera rostochiensis 7G
Heterodera goettingiana 1 Globodera rostochiensis 7 Globodera rostochiensis 1G Globodera rostochiensis 2G Globodera rostochiensis 3G
1
Pratylenchidae - Nacobbinae
0.9
Globodera rostochiensis 3 Globodera rostochiensis 4 Globodera rostochiensis 5 Globodera rostochiensis 6
1
Tylenchorhynchus teeni 2 Tylenchorhynchus teeni 1
Nacobbus aberrans 7G Nacobbus aberrans 8G
0.66
0.87
1
0.99 Hoplolaimus galeatus 1G Hoplolaimus galeatus 2G
1
Helicotylenchus pseudodigonicus 1 Helicotylenchus pseudodigonicus 2 Helicotylenchus pseudodigonicus 3
Helicotylenchus vulgaris 2 Helicotylenchus vulgaris 3 Helicotylenchus vulgaris 4 Helicotylenchus vulgaris 1
1 Hoplolaimus galeatus 3G Hoplolaimus galeatus 4G
Rotylenchus urmiaensis 1G Rotylenchus urmiaensis 2G 1 1 Rotylenchus cretensis 1G Rotylenchus striaticeps 1G Rotylenchus iranicus 1G Rotylenchus fallorobustus 1 Rotylenchus fallorobustus 3 Rotylenchus goodeyi 1 Rotylenchus robustus 1 Rotylenchus uniformis 1 1 Rotylenchus robustus 2 Rotylenchus uniformis 3 Rotylenchus robustus 1G Rotylenchus uniformis 1G Rotylenchus jaeni 1G Rotylenchus cypriensis 1G Rotylenchus eximius 1G Hoplolaimus columbus 1G Hoplolaimus columbus 2G
Neodolichodorus sp. 1G
Globodera pallida 1 Globodera pallida 7 Globodera pallida 1G Globodera mexicana 1 Globodera pallida 3 Globodera pallida 9 Globodera pallida 6 Globodera pallida 10 Globodera sp. 1 Globodera pallida 8 Globodera pallida 11 Globodera sp. 2 Globodera pallida 2G Globodera achilleae 1 Globodera achilleae 5 0.96 Globodera achilleae 2 Globodera artemisiae 1 Globodera achilleae 3 Globodera artemisiae 1G 0.8 Globodera achilleae 4 Punctodera stonei 1 Punctodera sp. 1G Vittatidera zeaphila 1G 1
0.63
Tylenchorhynchus dubius 1 Tylenchorhynchus dubius 1G
1
Hirschmanniella sp. 1 Hirschmanniella sp. 2 Hirschmanniella sp. 3
Pratylenchus thornei 5 Pratylenchus thornei 2G
Pratylenchus vulnus 1 Pratylenchus vulnus 2
Pratylenchus pratensis 1 Pratylenchus pratensis 2 Pratylenchus pratensis 3
Pratylenchus crenatus 1G Pratylenchus crenatus 2G Pratylenchus crenatus 3G 1
1
1
1
Nacobbus aberrans 1 Nacobbus aberrans 1G Nacobbus aberrans 2G
Nacobbus aberrans 5G Nacobbus aberrans 6G
Hirschmanniella cf. belli 1G Hirschmanniella sp. 1G 1 Hirschmanniella pomponiensis 1G Hirschmanniella santarosae 1G Hirschmanniella gracilis 1 1 Hirschmanniella gracilis 2 Hirschmanniella gracilis 3
1
Nacobbus aberrans 3G Nacobbus aberrans 4G
0.64
0.69
Rotylenchus vitis 1G
0.62
0.77 0.96
Morulaimus sp. 1G
Helicotylenchus vulgaris 1G
Ectoparasites
1
1
Dolichodoridae
1
Pratylenchus crenatus 2 Pratylenchus crenatus 3 Pratylenchus crenatus 4 Pratylenchus crenatus 1
Pratylenchus scribneri 1 1 Pratylenchus scribneri 2 Pratylenchus hippeastri 1G
1
1
Pratylenchus japonicus 1G
1
1
0.66
Hirschmanniella loofi 1G Hirschmanniella mucronata 1G Hirschmanniella mucronata 2G Hirschmanniella oryzae 1G Hirschmanniella oryzae 2G
1
0.97
0.69
0.69
Belonolaimidae
1
0.83
Pratylenchus bolivianus 1
Zygotylenchus guevarai 1G
0.58
1
Trophurus imperialis 2 Trophurus lomus 1 Trophurus sculptus 1
Carphodorus sp. 1G Carphodorus sp. 2G
Hoplotylus femina 1
1
Ectoparasites
Neodolichorhynchus lamelliferus 1 Bitylenchus bryobius 1 Macrotrophurus arbusticola 1 Macrotrophurus arbusticola 2
1
Neodolichorhynchus microphasmis 2 Sauertylenchus maximus 1 Sauertylenchus maximus 3 Sauertylenchus maximus 2 Sauertylenchus maximus 1G
1 Ibipora lolii 1G Ibipora lolii 2G Belonolaimus longicaudatus 5G Belonolaimus longicaudatus 6G
Telotylenchus ventralis 1
Belonolaimus longicaudatus 1G Belonolaimus longicaudatus 2G Belonolaimus longicaudatus 4G
1
Telotylenchidae
0.63
Supporting information - S4 Fig - part A Clade 12, Tylenchida - SSU rDNA
0.81
0.81
0.81
Bayesian inference incl sec struct 10,000,000 generations
0.56
Number of taxa: 1,089 + 10
0.87
Clade 12B
Holterman, Karegar and Mooijman et al.
88
35
83
100
77
60
21
33
36
96
40
Telotylenchidae
77
Supporting Information - S4 Fig - part B
88
67
Clade 12, Tylenchida - SSU rDNA RAxML incl sec struct
57
23
Number of taxa: 1,089 + 10
99
clade 12B
32
100
90
47
3
9
6
12
22
27
100
97
100
Trophurus ussuriensis 1 Trophurus sculptus 1 100 Trophurus ussuriensis 2 Trophurus lomus 1 Tylenchorhynchus claytoni 1G Tylenchorhynchus claytoni 2G
100
HOPLOLAIMINA
57
43
Telotylenchus ventralis 1
100 Carphodorus sp. 1G Carphodorus sp. 2G
43
44
71
100
100 83
100
100
73
99
100
Pratylenchus japonicus 1G
Zygotylenchus guevarai 1G Pratylenchus bolivianus 1
69
84
48
100
25
100
44
17
8
4
18
29
29
32
99
62
100 42
Pratylenchus thornei 1Pratylenchus thornei 5 Pratylenchus thornei 3Pratylenchus thornei 2G Pratylenchus thornei 4
81
77
36 99
53
100
100
100
100
25
Helicotylenchus minzi 1 Helicotylenchus minzi 4 Helicotylenchus platyurus 1 Helicotylenchus minzi 2 Helicotylenchus minzi 5 Helicotylenchus platyurus 2 Helicotylenchus minzi 3 Helicotylenchus varicaudatus 1G Helicotylenchus sp. 2 Helicotylenchus minzi 1G Helicotylenchus sp. 1G Helicotylenchus minzi 2G Helicotylenchus sp. 2G Helicotylenchus digonicus 1G Helicotylenchus sp. 3G Helicotylenchus minzi 3G 99 Helicotylenchus minzi 4G
100 84
75
100
Pratylenchus oleae 1G Pratylenchus oleae 2G
Pratylenchus goodeyi 1G
100
63
93
2
38
32
10 8
26
9
16
5
34
13
75
37
73 56
100 Meloidogyne duytsi 1G
99
Meloidogyne incognita 12G Meloidogyne incognita 13G Meloidogyne incognita 14G Meloidogyne incognita 15G Meloidogyne arenaria 1 Meloidogyne arenaria 1G Meloidogyne arenaria 2G Meloidogyne arenaria 3G
Meloidogyne incognita 17G Meloidogyne cruciani 1G Meloidogyne incognita 18G Meloidogyne floridensis 1G Meloidogyne lopezi 1G Meloidogyne morocciensis 1G Meloidogyne sp. 8 Meloidogyne arenaria 11G Meloidogyne sp. 9 Meloidogyne arenaria 13G Meloidogyne sp. 10 Meloidogyne arenaria 14G Meloidogyne sp. 2G Meloidogyne sp. 3G
Meloidogyne arenaria 4G Meloidogyne arenaria 5G Meloidogyne arenaria 6G Meloidogyne arenaria 8G
Meloidogyne hapla 6G
Meloidogyne graminis 15G
Meloidogynidae
Meloidogyne spartinae 1G Meloidogyne graminis 1G Meloidogyne graminis 5G 58 Meloidogyne graminis 2G Meloidogyne graminis 6G Meloidogyne graminis 3G Meloidogyne graminis 7G 35 Meloidogyne graminis 4G Meloidogyne graminis 14G 40 Meloidogyne graminis 12G Meloidogyne marylandi 1G Meloidogyne marylandi 2G Meloidogyne marylandi 5G Meloidogyne graminis 9G Meloidogyne graminis 8G Meloidogyne marylandi 3G Meloidogyne marylandi 4G Meloidogyne graminis 10G Meloidogyne graminis 13G Meloidogyne graminis 11G
90 86 Meloidogyne hapla 2G Meloidogyne hapla 5 Meloidogyne hapla 6 Meloidogyne hapla 12
44
100
Meloidogyne maritima 1Meloidogyne maritima 2 Meloidogyne maritima 1G
Meloidogyne ardenensis 1 Meloidogyne ardenensis 2
98
Meloidogyne hapla 4 Meloidogyne hapla 11 Meloidogyne cf. hapla 8 Meloidogyne hapla 3G Meloidogyne hapla 9 Meloidogyne hapla 4G Meloidogyne hapla 10 Meloidogyne hapla 5G
Meloidogyne ethiopica 1G Meloidogyne ethiopica 6G Meloidogyne hispanica 1G Meloidogyne ethiopica 2G Meloidogyne ethiopica 7G Meloidogyne incognita 19G Meloidogyne ethiopica 4G Meloidogyne ethiopica 8G Meloidogyne ethiopica 5G Meloidogyne sp. 1G Meloidogyne ethiopica 3G Meloidogyne incognita 1 Meloidogyne incognita 2G Meloidogyne incognita 7G Meloidogyne incognita 2 Meloidogyne incognita 3G Meloidogyne incognita 9G Meloidogyne incognita 3 Meloidogyne incognita 4G Meloidogyne incognita 10G 40 Meloidogyne incognita 1G Meloidogyne incognita 5G Meloidogyne incognita 11G Meloidogyne javanica 1 Meloidogyne javanica 6G Meloidogyne javanica 10G Meloidogyne javanica 1G Meloidogyne javanica 7G Meloidogyne javanica 11G Meloidogyne javanica 2G Meloidogyne javanica 8G Meloidogyne javanica 12G Meloidogyne javanica 3G Meloidogyne javanica 9G Meloidogyne lopezi 2G Meloidogyne arabicida 2G Meloidogyne izalcoensis 1G Meloidogyne lopezi 3G Meloidogyne arabicida 3G Meloidogyne izalcoensis 2G Meloidogyne lopezi 4G Meloidogyne arabicida 4G Meloidogyne lopezi 5G Meloidogyne paranaensis 1G
Meloidogyne arabicida 1G Meloidogyne konaensis 1G Meloidogyne sp. 4G
54
37
12
Meloidogyne arenaria 5 Meloidogyne incognita 8G Meloidogyne javanica 4G Meloidogyne incognita 6G Meloidogyne javanica 2 100 Meloidogyne duytsi 1 Meloidogyne javanica 5G 47 Meloidogyne duytsi 2 Meloidogyne microtyla 1G 44
62
Meloidogyne incognita 4
95
95
100 Dolichodorus sp. 1G Dolichodorus sp. 2G Dolichodorus heterocephalus 1G
Meloidogyne artiellia 1 Meloidogyne artiellia 1G 100 Meloidogyne artiellia 2 Meloidogyne artiellia 2G Meloidogyne mali 1 Meloidogyne ulmi 1 Meloidogyne mali 2 Meloidogyne ulmi 4 Meloidogyne mali 1G Meloidogyne ichinohei 1 Meloidogyne ichinohei 4 100 Meloidogyne ichinohei 2 Meloidogyne ichinohei 1G Meloidogyne ichinohei 3 Meloidogyne sp. 1 100 Meloidogyne sp. 2 Meloidogyne sp. 4
Radopholus similis 3 Radopholus similis 1G Radopholus similis 2G
Radopholus similis 3G Radopholus bridgei 1G Radopholus sp. 2
0.2
Ectoparasites
Pratylenchidae - Radopholinae
Dolichodoridae
Sedentary endoparasites - 1.
Meloidogyne enterolobii 1G Meloidogyne enterolobii 5G Meloidogyne enterolobii 9G Meloidogyne enterolobii 15G Meloidogyne enterolobii 20G Meloidogyne enterolobii 24G Meloidogyne enterolobii 28G Meloidogyne enterolobii 2G Meloidogyne enterolobii 6G Meloidogyne enterolobii 10G Meloidogyne enterolobii 17G Meloidogyne enterolobii 21G Meloidogyne enterolobii 25G Meloidogyne enterolobii 29G Meloidogyne enterolobii 3G Meloidogyne enterolobii 7G Meloidogyne enterolobii 11G Meloidogyne enterolobii 18G Meloidogyne enterolobii 22G Meloidogyne enterolobii 26G Meloidogyne mayaguensis 1G Meloidogyne enterolobii 4G Meloidogyne enterolobii 8G Meloidogyne enterolobii 12G Meloidogyne enterolobii 19G Meloidogyne enterolobii 23G Meloidogyne enterolobii 27G Meloidogyne chitwoodi 1 Meloidogyne chitwoodi 5 Meloidogyne chitwoodi 9 Meloidogyne chitwoodi 1G Meloidogyne chitwoodi 5G Meloidogyne chitwoodi 9G Meloidogyne chitwoodi 13G Meloidogyne chitwoodi 2 Meloidogyne chitwoodi 6 Meloidogyne chitwoodi 10 Meloidogyne chitwoodi 2G Meloidogyne chitwoodi 6G Meloidogyne chitwoodi 10G Meloidogyne chitwoodi 14G Meloidogyne chitwoodi 3 Meloidogyne chitwoodi 7 Meloidogyne chitwoodi 11 Meloidogyne chitwoodi 3G Meloidogyne chitwoodi 7G Meloidogyne chitwoodi 11G Meloidogyne chitwoodi 15G Meloidogyne chitwoodi 4 Meloidogyne chitwoodi 8 Meloidogyne chitwoodi 12 Meloidogyne chitwoodi 4G Meloidogyne chitwoodi 8G Meloidogyne chitwoodi 12G Meloidogyne chitwoodi 16G 49 Meloidogyne fallax 1 Meloidogyne fallax 10 Meloidogyne fallax 14 Meloidogyne fallax 2G Meloidogyne fallax 6G Meloidogyne fallax 10G Meloidogyne fallax 14G Meloidogyne fallax 6 Meloidogyne fallax 11 Meloidogyne fallax 15 Meloidogyne fallax 3G Meloidogyne fallax 7G Meloidogyne fallax 11G Meloidogyne fallax 7 Meloidogyne fallax 12 Meloidogyne fallax 16 Meloidogyne fallax 4G Meloidogyne fallax 8G Meloidogyne fallax 12G 96 Meloidogyne fallax 9 Meloidogyne fallax 13 Meloidogyne fallax 1G Meloidogyne fallax 5G Meloidogyne fallax 9G Meloidogyne fallax 13G Meloidogyne minor 1 Meloidogyne minor 5G Meloidogyne minor 2 Meloidogyne minor 6G 93 Meloidogyne minor 1G Meloidogyne minor 7G 74 Meloidogyne minor 4G Meloidogyne minor 8G Meloidogyne graminicola 2G Meloidogyne graminicola 6G 63 Meloidogyne graminicola 3G Meloidogyne graminicola 7G Meloidogyne graminicola 4G Meloidogyne graminicola 8G Meloidogyne graminicola 5G Meloidogyne oryzae G Meloidogyne graminicola 1G 55 Meloidogyne naasi 1 Meloidogyne naasi 2G Meloidogyne naasi 6G Meloidogyne kralli 1 31 35 Meloidogyne naasi 2 Meloidogyne naasi 3G Meloidogyne naasi 7G Meloidogyne naasi 3 Meloidogyne naasi 4G Meloidogyne naasi 8G Meloidogyne naasi 4 Meloidogyne naasi 5G Meloidogyne naasi 9G Meloidogyne exigua 1G Meloidogyne exigua 3G
Meloidogyne enterolobii 13G Meloidogyne enterolobii 14G Meloidogyne enterolobii 16G
100
Meloidogyne exigua 2G
Meloidogyne coffeicola 1G
Migratory endoparasites
Rotylenchulus reniformis genotype 7 consensus Rotylenchulus reniformis genotype 9 consensus
100
Ectoparasites / Semi-endoparasites
Sedentary semi-endoparasites - 3.
Migratory endoparasites
Helicotylenchus pseudorobustus genotype 1 consensus 99 Helicotylenchus certus 1 Helicotylenchus certus 2 19 Helicotylenchus dihystera 1G Helicotylenchus dihystera 5G Helicotylenchus dihystera 9G 97 Helicotylenchus dihystera 2G Helicotylenchus dihystera 6G Helicotylenchus dihystera 10G Helicotylenchus dihystera 3G Helicotylenchus dihystera 7G Helicotylenchus rotundicauda 1G Helicotylenchus dihystera 4G Helicotylenchus dihystera 8G Helicotylenchus sp. 6 20 100 Helicotylenchus multicinctus 1 Helicotylenchus multicinctus 2G 35 Helicotylenchus multicinctus 1G Helicotylenchus multicinctus 3G 28 Helicotylenchus crenacauda 1G 98 Helicotylenchus paraplatyurus 1G 24 Helicotylenchus paraplatyurus 2G 100 Helicotylenchus indicus 1 Helicotylenchus indicus 3 Helicotylenchus indicus 2 Helicotylenchus indicus 4 Helicotylenchus digitiformis 1 Helicotylenchus digitiformis 6 59 Helicotylenchus digitiformis 2 Helicotylenchus pseudorobustus genotype 2 consensus Helicotylenchus digitiformis 4 Helicotylenchus sp. 5 Helicotylenchus cf. digitiformis 8 Helicotylenchus cf. digitiformis 7 Helicotylenchus sp. 1
Rotylenchulidae
100 Rotylenchulus borealis 1 Rotylenchulus borealis 2 Rotylenchulus reniformis genotype 1 consensus Rotylenchulus reniformis genotype 6 consensus
Sedentary endoparasites - 3.
Pratylenchus penetrans 1 Pratylenchus convallariae 1 Pratylenchus scribneri 4 Pratylenchus penetrans 2 Pratylenchus convallariae 2 Pratylenchus scribneri 5 Pratylenchus penetrans 1G Pratylenchus fallax 1 Pratylenchus cf. penetrans 2G
Pratylenchidae - Pratylenchinae, Hirschmanniellinae
Pratylenchus neglectus 1 Pratylenchus neglectus 1G Pratylenchus neglectus 5G Pratylenchus neglectus 2 Pratylenchus neglectus 2G Pratylenchus neglectus 6G Pratylenchus neglectus 3 Pratylenchus neglectus 3G Pratylenchus neglectus 4 Pratylenchus neglectus 4G
Pratylenchus araucensis 1G Pratylenchus crenatus 2 Pratylenchus pratensis 1G Pratylenchus crenatus 3 Pratylenchus pratensis 2G Pratylenchus crenatus 4 Pratylenchus crenatus 1
Pratylenchus crenatus 1G Pratylenchus crenatus 2G Pratylenchus crenatus 3G 100
Pratylenchidae - Nacobbinae
85
Heteroderidae
Globodera artemisiae 2 43 Heterodera avenae 1 Heterodera filipjevi 1 Heterodera filipjevi 3G 93 Heterodera avenae 2 Heterodera filipjevi 2 Heterodera filipjevi 4G Heterodera avenae 3 Heterodera filipjevi 1G Heterodera mani 1 31 Heterodera avenae 4 Heterodera filipjevi 2G Heterodera sp. 1 100 73 Heterodera elachista 1G Heterodera elachista 2G Heterodera glycines 2G Heterodera glycines 6G Heterodera trifolii 1 Heterodera schachtii 1 Heterodera schachtii 5 98 Heterodera glycines 3G Heterodera glycines 7G Heterodera trifolii 2 Heterodera schachtii 2 Heterodera schachtii 6 42 Heterodera trifolii 4 Heterodera schachtii 3 Heterodera schachtii 1G Heterodera glycines 4G Heterodera betae 1 Heterodera trifolii 5 Heterodera schachtii 4 Heterodera hordecalis 1 Heterodera glycines 5G Heterodera betae 2 88 Punctodera punctata 1 Afenestrata koreana 1G Heterodera goettingiana 1 Globodera rostochiensis 3 Globodera rostochiensis 7 Globodera rostochiensis 4G Globodera tabacum 3 Globodera rostochiensis 4 Globodera rostochiensis 1G Globodera rostochiensis 5G Globodera tabacum 4 Globodera rostochiensis 5 Globodera rostochiensis 2G Globodera rostochiensis 6G Globodera rostochiensis 6 Globodera rostochiensis 3G Globodera rostochiensis 7G
Sedentary endoparasites - 2.
Pratylenchus pratensis 1 Pratylenchus pratensis 4 100 Pratylenchus pratensis 2 Pratylenchus pratensis 5 Pratylenchus pratensis 3 100 Pratylenchus vulnus 1 Pratylenchus vulnus 3 Pratylenchus vulnus 2 Pratylenchus vulnus 4 Hirschmanniella pomponiensis 1G Hirschmanniella santarosae 1G Hirschmanniella gracilis 1 Hirschmanniella gracilis 2 Hirschmanniella gracilis 3 Hirschmanniella sp. 1 Hirschmanniella loofi 1 Hirschmanniella sp. 2 Hirschmanniella n. sp. 1 Hirschmanniella sp. 3
99
Pratylenchus scribneri 1 Pratylenchus scribneri 2 Pratylenchus hippeastri 1G
80
100
Cactodera sp. 1GCactodera sp. 3G Cactodera sp. 2G Cryphodera sinensis 1G Cryphodera brinkmani 1G
Globodera pallida 1 Globodera pallida 7 Globodera pallida 1G Globodera sp. 5 Globodera sp. 4 Globodera pallida 3 Globodera pallida 9 Globodera sp. 1 Globodera sp. 6 Globodera pallida 6 Globodera pallida 10 Globodera sp. 2 Globodera sp. 7 Globodera pallida 8 Globodera pallida 11 Globodera sp. 3 Globodera sp. 8
75
47
Helicotylenchus digitiformis 3
Hoplolaimidae
74 92 95 Rotylenchulus reniformis genotype 8 consensus 32 Rotylenchulus reniformis genotype 3 consensus 100 Rotylenchulus reniformis genotype 2 consensus Rotylenchulus reniformis genotype 4 consensus Rotylenchulus reniformis genotype 5 consensus Rotylenchus sp. 1 100 Scutellonema bradys 1G Scutellonema bradys 2G Helicotylenchus digonicus 6G 100 Helicotylenchus digonicus 8G Helicotylenchus digonicus 7G
Dolichodoridae
Neodolichodorus sp. 1G
100
98
Helicotylenchus vulgaris 2 Helicotylenchus canadensis 1 Helicotylenchus sp. 3 Helicotylenchus vulgaris 3 Helicotylenchus canadensis 2 Helicotylenchus sp. 4 Helicotylenchus vulgaris 4 Helicotylenchus digonicus 1 Helicotylenchus vulgaris 1 Helicotylenchus pseudodigonicus 1 Helicotylenchus pseudodigonicus 4 Helicotylenchus pseudodigonicus 2 Helicotylenchus pseudodigonicus 5 Helicotylenchus pseudodigonicus 3 Helicotylenchus scoticus 1G Helicotylenchus vulgaris 1G 78
100 Hoplolaimus galeatus 1G Hoplolaimus galeatus 3G Hoplolaimus galeatus 2G Hoplolaimus galeatus 4G
100 Rotylenchus urmiaensis 1G 94 Rotylenchus urmiaensis 2G 100 Rotylenchus cretensis 1G Rotylenchus striaticeps 1G Rotylenchus iranicus 1G Rotylenchus fallorobustus 1 Rotylenchus fallorobustus 3 Rotylenchus goodeyi 1 Rotylenchus robustus 1 Rotylenchus uniformis 1 Rotylenchus robustus 1G Rotylenchus robustus 2 Rotylenchus uniformis 3 Rotylenchus uniformis 1G Rotylenchus jaeni 1G Rotylenchus cypriensis 1G Rotylenchus eximius 1G Hoplolaimus columbus 1G 100 Hoplolaimus columbus 2G 88
Globodera pallida 2G Globodera achilleae 1Globodera achilleae 5 Globodera achilleae 2Globodera artemisiae 1 Globodera achilleae 3Globodera artemisiae 1G Globodera achilleae 4 Punctodera stonei 1 Punctodera sp. 1G Vittatidera zeaphila 1G 100 73
25
71
14 53
96
100
26
100
28
64
Morulaimus sp. 1G
Ectoparasites
94
37 47 46
Rotylenchus vitis 1G
56
Tylenchorhynchus dubius 1 Tylenchorhynchus teeni 1 Tylenchorhynchus dubius 1G Tylenchorhynchus teeni 2 100 Nacobbus aberrans 3G Nacobbus aberrans 5G Nacobbus aberrans 4G Nacobbus aberrans 6G 100 Nacobbus aberrans 1 Nacobbus aberrans 7G Nacobbus aberrans 1G Nacobbus aberrans 8G Nacobbus aberrans 2G
Hirschmanniella mucronata 1G Hirschmanniella mucronata 2G Hirschmanniella loofi 1G Hirschmanniella oryzae 1G Hirschmanniella cf. belli 1G Hirschmanniella oryzae 2G Hirschmanniella sp. 1G Trophurus imperialis 2
36
58
37
Belonolaimidae
Hoplotylus femina 1
100
Ectoparasites
Neodolichorhynchus lamelliferus 1 98 Bitylenchus bryobius 1 Macrotrophurus arbusticola 1 Macrotrophurus arbusticola 2 Neodolichorhynchus microphasmis 2 Sauertylenchus maximus 1 Sauertylenchus maximus 3 Sauertylenchus maximus 2 Sauertylenchus maximus 1G
74
100 Ibipora lolii 1G Ibipora lolii 2G
Belonolaimus longicaudatus 1G Belonolaimus longicaudatus 5G Belonolaimus longicaudatus 2G Belonolaimus longicaudatus 6G Belonolaimus longicaudatus 3G Belonolaimus longicaudatus 7G Belonolaimus longicaudatus 4G Belonolaimus longicaudatus 8G
1
1
0.68
0.5
0.77
0.78
1
1
1
0.54
0.93
1
1
0.68
1
0.71
1
1
0.67
0.81
0.96
0.64 0.94
0.72
1
0.82
Migratory endoparasites
Scutylenchus rugosus 1 Scutylenchus rugosus 2
Amplimerlinius macrurus 1 Amplimerlinius icarus 1G Amplimerlinius macrurus 2 Amplimerlinius globigerus 2 Amplimerlinius macrurus 3 Nagelus obscurus 1 Nagelus obscurus 1G 1 Nagelus obscurus 2
1
1 1
0.79
1
1
Basiria duplexa 1 Basiria duplexa 2 Basiria sp. 2
1
Basiria gracilis 1G Basiria gracilis 5
1
0.68
0.55
1
0.9
HOPLOLAIMINA
Anguinidae
1
Howardula sp. 2G 1
1
0.99
1
1
0.64
1
1 0.93
0.72
1
Ficotylus congestae 1G
1
1
Neotylenchidae
Ditylenchus sp. 1G
Disparate gain and loss patterns
Supporting information - S4 Fig. - part C
Clade 12, Tylenchida - SSU rDNA
Bayesian inference incl sec struct
10,000,000 generations
Number of taxa: 1,089 + 10 (S4 Fig)
Skarbilovinema lyoni 1G
Iotonchiidae
parasites / sedentary endoparasites - 4.
Hemicycliophoridae
Hemicycliophora thornei 1 Hemicycliophora thienemanni 2G Hemicycliophora thienemanni 11 Hemicycliophora sp. 1G Hemicycliophora thienemanni 2 Hemicycliophora sp. 2G Hemicycliophora thienemanni 1G
Mesocriconema xenoplax 2G Mesocriconema xenoplax 3G Mesocriconema xenoplax 4G
Mesocriconema sp. 1G Mesocriconema sphaerocephalum 1G Mesocriconema sp. 2G Mesocriconema sp. 3G Mesocriconema sp. 4G
Sakia sp. 1G Sakia sp. 2G
Filenchus sp. 1G
Malenchus neosulcus 1 Malenchus acarayensis 1
Lelenchus leptosoma 3
Ecphyadophoridae
Tylenchidae
Filenchus facultativus 1
Sedentary endoparasites - 5.
Sphaeronematidae
Ectoparasites
Insect parasites, most members feed on fungi and/or higher plants as well
Parasitylenchidae
Allantonematidae
Neotylenchidae Insect
Fergusobia sp. genotype 9 consensus Tylenchina sp. 1G
Howardula sp. 5G
Hemicaloosia vagisclera 2G Hemicaloosia sp. 1G
Caloosiidae
Parasitylenchus bifurcatus 1 Parasitylenchus bifurcatus 4 Parasitylenchus bifurcatus 2 Parasitylenchus bifurcatus 5 Parasitylenchus bifurcatus 3 1 Bradynema listronotum 1G Allantonematidae Bradynema listronoti 2G
1
Mesocriconema xenoplax 5G
Meloidoderita n. sp. 2 Meloidoderita n. sp. 3
Meloidoderita salina 2 Meloidoderita salina 3
Criconematidae
Paratylenchidae
1
Paratylenchus sp. 4G Paratylenchus sp. 8G Paratylenchus sp. 5G Paratylenchus sp. 9G Paratylenchus sp. 6G Paratylenchus sp. 10G Paratylenchus sp. 7G Paratylenchus sp. 11G Tylenchocriconema alleni 1
Tylenchidae
Filenchus andrassyi 1 Irantylenchus vicinus 1 Tylenchus davainei 1
Echtoparasites, root hair feeders, algal, lichen or moss feeders
Irantylenchus clavidorus 1 Filenchus sp. 3
Ecphyadophoridae
Paratylenchus colinus 1G Paratylenchus (Gracilacus) sp. 1G Paratylenchus sp. 1 0.96 Paratylenchus sp. 1G Paratylenchus sp. 2G Paratylenchus sp. 3G
Filenchus sp. 2G
Tylenchidae
1
Mesocriconema xenoplax 1 Mesocriconema xenoplax 2 Mesocriconema xenoplax 3 Mesocriconema xenoplax 1G
Hemicycliophora conida 1 Hemicycliophora conida 1G Hemicycliophora conida 2G Hemicycliophora conida 3G
0.74
1
Hemicriconemoides wessoni 1G Hemicriconemoides wessoni 2G
1
Hemicaloosia graminis 1G Hemicaloosia vagisclera 1G
Ogma sp. 1G
1
Neotylenchidae
1
Howardula neocosmis 1G Howardula cf. neocosmis 1G Howardula cf. neocosmis 2G Howardula sp. 4G
Fergusobia sp. genotype 5 consensus Fergusobia sp. genotype 6 consensus Fergusobia sp. genotype 7 consensus Fergusobia sp. genotype 8 consensus Howardula aoronymphium 1G Howardula aoronymphium 2G Howardula aoronymphium 3G Howardula cf. aoronymphium 1G Howardula sp. 1G
1
Howardula phyllotretae 1G Howardula dominicki 1G
Hexatylus sp. 1 Hexatylus sp. 2
1
1
Hemicriconemoides pseudobrachyurus 1 Hemicriconemoides pseudobrachyurus 2 Hemicriconemoides pseudobrachyurus 3
Deladenus siricidicola 4G Deladenus proximus consensus
0.88
1
1
Fergusobia sp. genotype 1 consensus Fergusobia sp. genotype 2 consensus Fergusobia sp. genotype 3 consensus Fergusobia sp. genotype 4 consensus
Deladenus siricidicola 1G Deladenus siricidicola 2G Deladenus siricidicola 3G Deladenus durus 1
1
Migratory endoparasites facultative or obligate plant parasites (above-ground plant parts)/ fungivores Halenchus fucicola 1
0.68
1
1
1
1
Ogma cobbi 1 1 1 Ogma menzeli 1 Criconema sp. 1G Ogma sp. 2G 1 Ogma sp. 1 Hemicriconemoides chitwoodi 1G Paratylenchus goldeni 1G 1 1 Paratylenchus microdorus 2 Paratylenchus similis 2 Paratylenchus microdorus 1 Paratylenchus conicephalus 1G 1 1 Paratylenchus projectus 1 1 Paratylenchus projectus 2 Paratylenchus dianthus 1G 1 Paratylenchus straeleni 2 Paratylenchus straeleni 3 Paratylenchus nanus 1 Paratylenchus cf. neoamblycephalus 1 1
0.66
Filenchus misellus 1
Ecphyadophora tenuissima 4 Ecphyadophora sp. 1
Tylenchulidae
1
Ecphyadophora tenuissima 1 Ecphyadophora tenuissima 2 Ecphyadophora tenuissima 3
1
Aphelenchidae
Filenchus chilensis 1
Tylenchulus semipenetrans 1G
1
1
1
Ditylenchus ferepolitor 1
Howardula sp. 3G
Genus dubium
0.94
cf. Helionema sp. 1
1
Ditylenchus angustus 1G
Ditylenchus sp. 6
0.97
1
Sphaeronema sp. 1 Sphaeronema alni 1G
1
Discotylenchus n. sp. 1
Filenchus discrepans 1 Filenchus discrepans 2
1
Aphelenchus avenae 4G Aphelenchus avenae 5G Aphelenchus sp. 1G
Filenchus aquilonius 2
Aphelenchus avenae 1 Aphelenchus avenae 2 Aphelenchus avenae 2G Aphelenchus avenae 3G
Tylenchidae
Malenchus andrassyi 1
1
Sphaerulariidae
0.56
0.77
Echtoparasites, root hair feeders, algal, lichen or moss feeders
1
1 1
1
Pseudhalenchus minutus 1 Pseudhalenchus minutus 2
Ditylenchus sp. 4
Tylenchidae sp. 1G
Ditylenchus adasi 1 Ditylenchus sp. 3 Ditylenchus adasi 2 Ditylenchus sp. 10 1 Ditylenchus sp. 8
1
Ditylenchus destructor 1G Ditylenchus sp. 2G 1
1
Deladenus sp. 1G Deladenus sp. 2G Neotylenchidae Nothotylenchus acris 1 Anguinidae
1
Tylenchus naranensis 1 Tylenchus naranensis 2
Ecphyadophoridae
Aphelenchus sp. 1
Tylodoridae
Lelenchus leptosoma 1
1
Tylenchidae - Boleodorinae
1
CRICONEMATINA
1 Sphaerularia vespae 1G Sphaerularia bombi 2G Sphaerularia bombi 1G
Ditylenchus destructor 1 Ditylenchus destructor 2 Ditylenchus destructor 3 Ditylenchus destructor 4
Ditylenchus drepanocercus 1G
1
Psilenchidae
Ditylenchus sp. 1 Ditylenchus sp. 5 Ditylenchus sp. 12
1
Anguinata sp. 1G
1
1
Filenchus longiurus 1 Filenchus longiurus 2
Basiria aberrans 2 Basiria aberrans 3 Basiria aberrans 4 Basiria aberrans 5
Filenchus sp. 5G Filenchus sp. 7G Filenchus sp. 8G
Boleodorus thylactus 4 Boleodorus thylactus 1G Boleodorus volutus 1
Filenchus thornei 3 Filenchus vulgaris 2 Filenchus sp. 3G Filenchus sp. 4G
0.98
Ectoparasites
Telotylenchidae - Merliniinae
Scutylenchus quadrifer 1 Scutylenchus quadrifer 1G Merlinius joctus 1 Merlinius brevidens 1
1
0.98
Ditylenchus dipsaci consensus 1 0.98 Ditylenchus sp. 2 Ditylenchus gigas 1G Subanguina radicicola 1 Litylenchus coprosma 1G 1 Subanguina radicicola 1G Ditylenchus dipsaci 1 Anguina tritici 1 Anguina tritici 2 Anguina agrostis 1G
1
1 1
Basiria sp. 1
Discotylenchus iranicus 1G Neopsilenchus magnidens 1 Neopsilenchus magnidens 3 Neopsilenchus magnidens 2
0.99
Ditylenchus sp. 11
0.88
1
Boleodorus thylactus 1 Boleodorus thylactus 2 Boleodorus thylactus 3
Filenchus vulgaris 3
Tylenchus arcuatus 1G
Tylenchus arcuatus 2G Filenchus sp. 4
Cephalenchus hexalineatus 1 Cephalenchus hexalineatus 2 Cephalenchus hexalineatus 4 Cephalenchus hexalineatus 3
Coslenchus rhombus 2 Coslenchus rhombus 3 Coslenchus sp. 2 Miculenchus salvus 1
Tylenchus arcuatus 1 Tylenchus cf. arcuatus 2 Tylenchus arcuatus 4 Coslenchus cf. franklinae 1 Coslenchus cf. pastor 1 Coslenchus cf. pastor 2 Coslenchus polonicus 3
Filenchus annulatus Filenchus aquilonius 1 Filenchus filiformis 1 Filenchus thornei 1
Paraphelenchus sp. 1
1
Aglenchus agricola 4 Aglenchus agricola 5
Coslenchus cancellatus 1 Coslenchus cancellatus 2 Coslenchus costatus 1 Coslenchus franklinae 2
Basiria graminophila 1 Basiria cf. aberrans 1
0.65
1
Coslenchus sp. 1 Aglenchus agricola 1 Aglenchus agricola 2 Aglenchus agricola 3
Cephalenchus leptus 1 Cephalenchus leptus 2
1
1
1
Echtoparasites, root hair feeders, algal, lichen or moss feeders
Tylenchidae
1
Pratylenchidae - Radopholinae
Pratylenchoides ritteri 1 0.95 Pratylenchoides ritteri 2 0.92 Pratylenchoides alkani 1 0.65 1 0.96 Pratylenchoides nevadensis 1 Pratylenchoides nevadensis 2 Pratylenchoides ritteri 1G Pratylenchoides magnicauda 1G 1 Pratylenchoides persicus 1 Pratylenchoides persicus 2 Pratylenchoides crenicauda 1 Pratylenchoides erzurumensis 1 Pratylenchoides crenicauda 2 Pratylenchoides sp. 1 Pratylenchoides crenicauda 3 0.81
0.89
0.59
0.99
1
1
Psilenchus hilarulus 5 Psilenchus sp. 1G Psilenchus hilarulus 6 Psilenchus hilarus 1 Psilenchus hilarulus 7 Psilenchus cf. hilarulus 1
Amplimerlinius globigerus 1 Amplimerlinius paraglobigerus 1 Amplimerlinius paraglobigerus 2 1 Paramerlinius hexagrammus 1 Paramerlinius hexagrammus 2
0.69
Psilenchus hilarulus 1 Psilenchus hilarulus 2 Psilenchus hilarulus 3 Psilenchus hilarulus 4
0.74
1
0.83
1
0.87
1
0.94
0.64
0.98
0.88
1
HEXATYLINA
0.9
0.99
1
Clade 12A
0.96
0.62
1
1
0.2
70
86
38
14
12
43
14
64
25
61
58
81
64
100
47
80
100
30
56
99
24
85
57
78
95
Clade 12A
26
83
16
100
62
100
42 51
HOPLOLAIMINA
48
93
97
91
88
Ditylenchus sp. 11
73
59
73
100
Telotylenchidae - Merliniinae
88
Tylenchidae sp. 1
99
67
Cephalenchus hexalineatus 1 Cephalenchus hexalineatus 3 Cephalenchus hexalineatus 2 Cephalenchus hexalineatus 4
Aphelenchus sp. 1
43
68
Ectoparasites
32
94
Howardula sp. 2G
Howardula phyllotretae 1G Howardula dominicki 1G
100
82
100
100
100
69
Ficotylus congestae 1G
Neotylenchidae
94
61
15
74
100
99
100
96
Disparate gain and loss patterns
Supporting information S4 Fig - part D
Clade 12, Tylenchida - SSU rDNA
RAxML incl sec struct
Number of taxa: 1,089 + 10 (Fig. 4A, B-2)
Skarbilovinema lyoni 1G
Iotonchiidae
Insect parasites, most members feed on fungi and/or higher plants as well
Hemicycliophoridae
Criconematidae
100 Sakia sp. 1G Sakia sp. 2G
Malenchus neosulcus 1
Filenchus sp. 1G
Malenchus acarayensis 1
Lelenchus leptosoma 3
Ecphyadophoridae
Filenchus facultativus 1
Sedentary endoparasites - 5.
Meloidoderita salina 2 Meloidoderita n. sp. 2 Meloidoderita salina 3 Meloidoderita n. sp. 3
Paratylenchidae
Sphaeronematidae 100
Ecphyadophoridae
Filenchus sp. 2G
Tylenchus davainei 1
Tylenchidae
0.2
Echtoparasites, root hair feeders, algal, lichen or moss feeders
Filenchus andrassyi 1 Irantylenchus vicinus 1
Ectoparasites
Mesocriconema sp. 1G Mesocriconema sphaerocephalum 1G Mesocriconema sp. 2G Mesocriconema sp. 4G Mesocriconema sp. 3G
Caloosiidae
Hemicycliophora thienemanni 1 Hemicycliophora thienemanni 2G Hemicycliophora sp. 2G 92 Hemicycliophora thienemanni 2 Hemicycliophora thornei 1 Hemicycliophora thienemanni 1G Hemicycliophora sp. 1G Hemicycliophora conida 1 Hemicycliophora conida 1G Hemicycliophora conida 3G Hemicycliophora conida 2G Ogma sp. 1G Criconematidae Hemicaloosia vagisclera 1G Hemicaloosia graminis 1G Hemicaloosia vagisclera 2G Hemicaloosia sp. 1G Mesocriconema xenoplax 1 Mesocriconema xenoplax 2G 60 Mesocriconema xenoplax 2 Mesocriconema xenoplax 3G Mesocriconema xenoplax 3 Mesocriconema xenoplax 4G 61 Mesocriconema xenoplax 1G 95
Mesocriconema xenoplax 5G
Insect parasites / sedentary endoparasites - 4.
41
47
100
100
Hemicriconemoides pseudobrachyurus 1 Hemicriconemoides wessoni 1G Hemicriconemoides pseudobrachyurus 2 Hemicriconemoides wessoni 2G Hemicriconemoides pseudobrachyurus 3 100 Ogma menzeli 1 96 Ogma cobbi 1 Criconema sp. 1G Hemicriconemoides chitwoodi 1G 99 Ogma sp. 2G Ogma sp. 1 Paratylenchus goldeni 1G 96 97 Paratylenchus microdorus 2 Paratylenchus similis 2 Paratylenchus microdorus 1 66 Paratylenchus conicephalus 1G 95 87 Paratylenchus projectus 2 94 Paratylenchus projectus 1 Paratylenchus dianthus 1G 100 Paratylenchus straeleni 3 Paratylenchus straeleni 2 Paratylenchus nanus 1 Paratylenchus cf. neoamblycephalus 1 100 Paratylenchus colinus 1G Paratylenchus (Gracilacus) sp. 1G Paratylenchus sp. 1 Paratylenchus sp. 4G Paratylenchus sp. 8G 75 Paratylenchus sp. 1G Paratylenchus sp. 5G Paratylenchus sp. 9G Paratylenchus sp. 2G Paratylenchus sp. 6G Paratylenchus sp. 10G 100 Paratylenchus sp. 3G Paratylenchus sp. 7G Paratylenchus sp. 11G Tylenchocriconema alleni 1
95
99
Irantylenchus clavidorus 1 Filenchus sp. 3
100
Ecphyadophora tenuissima 1 Ecphyadophora tenuissima 4 Ecphyadophora tenuissima 2 Ecphyadophora sp. 1 Ecphyadophora tenuissima 3 Filenchus misellus 1
Tylenchulidae
60
Parasitylenchidae
Allantonematidae
Neotylenchidae
97
81
Parasitylenchus bifurcatus 1 Parasitylenchus bifurcatus 4 Parasitylenchus bifurcatus 2 Parasitylenchus bifurcatus 5 Parasitylenchus bifurcatus 3 100 Bradynema listronoti 2G Allantonematidae Bradynema listronotum 1G
Neotylenchidae
100
100
98
93
Filenchus chilensis 1
Tylenchulus semipenetrans 1G
Filenchus discrepans 1 Filenchus discrepans 2
Discotylenchus n. sp. 1 100
Sphaeronema sp. 1 Sphaeronema alni 1G
Malenchus andrassyi 1
81 46
100
Fergusobia sp. genotype 1 consensus Fergusobia sp. genotype 5 consensus Fergusobia sp. genotype 9 consensus Fergusobia sp. genotype 2 consensus Fergusobia sp. genotype 6 consensus Tylenchid sp. 1 Fergusobia sp. genotype 3 consensus Fergusobia sp. genotype 7 consensus Fergusobia sp. genotype 4 consensus Fergusobia sp. genotype 8 consensus Howardula aoronymphium 1G Howardula cf. aoronymphium 1G Howardula neocosmis 1G 100 Howardula sp. 4G Howardula aoronymphium 2G Howardula cf. neocosmis 1G Howardula sp. 5G Howardula aoronymphium 3G Howardula cf. neocosmis 2G Howardula sp. 1G
Anguinidae
100
97
100
96
Deladenus siricidicola 1G Deladenus siricidicola 4G Deladenus siricidicola 2G Deladenus proximus consensus Deladenus siricidicola 3G Deladenus durus 1 100 Hexatylus sp. 1 Hexatylus sp. 2
100
Ditylenchus sp. 1G
Migratory endoparasites facultative or obligate plant parasites (above-ground plant parts) / fungivores
91
Genus dubium
100
Howardula sp. 3G
39
Ditylenchus ferepolitor 1
Halenchus fucicola 1
81
44
Ditylenchus angustus 1G
30
24
cf. Helionema sp. 1
99
84
CRICONEMATINA
100
100
51
92
Filenchus longiurus 1 Filenchus longiurus 2
Aphelenchus sp. 1G
Filenchus aquilonius 2
Tylenchidae - Boleodorinae
100
Sphaerulariidae
Anguinidae
Ditylenchus sp. 4
100
100
Pseudhalenchus minutus 1 Pseudhalenchus minutus 2
Ditylenchus sp. 6
Ectoparasites, root hair feeders, algal, lichen or moss feeders
"Pratylenchidae - Radopholinae"
Psilenchidae
75
71
100 Deladenus sp. 1G Nothotylenchus acris 1 Deladenus sp. 2G Sphaerularia bombi 1G Sphaerularia vespae 1G Sphaerularia bombi 2G
Ditylenchus destructor 1 Ditylenchus destructor 4 Ditylenchus destructor 2 Ditylenchus destructor 1G Ditylenchus destructor 3 Ditylenchus sp. 2G
Ditylenchus drepanocercus 1G
98
Ditylenchus sp. 1 Ditylenchus sp. 12 Ditylenchus sp. 5
100
Anguinid sp. 1G
45
69
Migratory endoparasites
49 Scutylenchus rugosus 1 Scutylenchus quadrifer 1 60 Scutylenchus rugosus 2 Scutylenchus quadrifer 1G 56 Merlinius joctus 1 Merlinius brevidens 1 21 63 Amplimerlinius paraglobigerus 1 Amplimerlinius paraglobigerus 2 Amplimerlinius globigerus 1 100 Paramerlinius hexagrammus 1 35 34 Paramerlinius hexagrammus 2 Amplimerlinius macrurus 1 Amplimerlinius icarus 1G 70 Amplimerlinius macrurus 2 Amplimerlinius globigerus 2 55 Amplimerlinius macrurus 3 100 Nagelus obscurus 1 Nagelus obscurus 1G 65 Pratylenchoides ritteri 1 Nagelus obscurus 2 66 Pratylenchoides ritteri 2 35 62 Pratylenchoides alkani 1 100 Pratylenchoides nevadensis 2 56 Pratylenchoides nevadensis 1 25 Pratylenchoides ritteri 1G Pratylenchoides magnicauda 1G 99 Pratylenchoides persicus 2 51 Pratylenchoides persicus 1 Pratylenchoides sp. 1 99 Pratylenchoides crenicauda 3 Pratylenchoides crenicauda 1 Pratylenchoides crenicauda 2 Pratylenchoides erzurumensis 1 87 Psilenchus hilarulus 1 Psilenchus hilarulus 5 Psilenchus sp. 1G Psilenchus hilarulus 2 Psilenchus hilarulus 6 Psilenchus hilarulus 3 Psilenchus hilarulus 7 Psilenchus hilarulus 4 Psilenchus cf. hilarulus 1 Psilenchus hilarus 1 91 Ditylenchus dipsaci consensus 95 Ditylenchus sp. 2 81 Ditylenchus gigas 1G 92 Subanguina radicicola 1 Litylenchus coprosma 1G Subanguina radicicola 1G Ditylenchus dipsaci 1 Anguina tritici 1 Anguina agrostis 1G Anguina tritici 2 100 Ditylenchus adasi 1 Ditylenchus sp. 3 Ditylenchus adasi 2 Ditylenchus sp. 10 100 100 Ditylenchus sp. 8
75
99
Tylodoridae
Boleodorus thylactus 1 Boleodorus thylactus 4 100 Boleodorus thylactus 2 Boleodorus thylactus 1G Boleodorus thylactus 3 Boleodorus volutus 1 100 Basiria aberrans 2 Basiria aberrans 5 Basiria aberrans 3 Basiria gracilis 5 Basiria aberrans 4 Basiria gracilis 1G 100 Basiria cf. aberrans 1 Basiria graminophila 1 Basiria sp. 1 69 Basiria duplexa 1 Basiria sp. 2 Basiria duplexa 2 Discotylenchus iranicus 1G Neopsilenchus magnidens 1 Neopsilenchus magnidens 3 Neopsilenchus magnidens 2
100
Paraphelenchus sp. 1
Aphelenchus avenae 1 Aphelenchus avenae 3G Aphelenchus avenae 2 Aphelenchus avenae 4G Aphelenchus avenae 2G Aphelenchus avenae 5G
Cephalenchus leptus 1 Cephalenchus leptus 2
100 Tylenchus naranensis 1 82 Tylenchus naranensis 2 Filenchus vulgaris 3 58 Filenchus thornei 1 Filenchus aquilonius 1 Filenchus sp. 5G 34 Filenchus thornei 3 Filenchus annulatus Filenchus sp. 7G Filenchus vulgaris 2 Filenchus sp. 3G 71 Filenchus sp. 8G Filenchus filiformis 1 Filenchus sp. 4G Tylenchus arcuatus 1 Tylenchus arcuatus 2G 75 Tylenchus arcuatus 4 Tylenchus cf. arcuatus 2 Tylenchus arcuatus 1G Filenchus sp. 4 Coslenchus cf. pastor 1 Coslenchus cancellatus 1 Coslenchus polonicus 3 Miculenchus salvus 1 Coslenchus cf. pastor 2 Coslenchus cancellatus 2 Coslenchus costatus 1 Coslenchus rhombus 2 Coslenchus cf. franklinae 1 Coslenchus sp. 2 Lelenchus leptosoma 1Ecphyadophoridae Coslenchus rhombus 3 Coslenchus franklinae 2 Coslenchus sp. 1 Aglenchus agricola 1 Aglenchus agricola 4 Aglenchus agricola 2 Aglenchus agricola 5 Aglenchus agricola 3
98
HEXATYLINA
61
27
98
92
100
