Saccharomyces cerevisiae - CiteSeerX

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MOLECULAR AND CELLULAR BIOLOGY, May 2005, p. 4075–4091 0270-7306/05/$08.00⫹0 doi:10.1128/MCB.25.10.4075–4091.2005 Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Vol. 25, No. 10

Dynamical Remodeling of the Transcriptome during Short-Term Anaerobiosis in Saccharomyces cerevisiae: Differential Response and Role of Msn2 and/or Msn4 and Other Factors in Galactose and Glucose Media† Liang-Chuan Lai,1 Alexander L. Kosorukoff,2 Patricia V. Burke,1 and Kurt E. Kwast1* Department of Molecular & Integrative Physiology1 and Department of Computer Science,2 University of Illinois, Urbana, Illinois Received 16 September 2004/Returned for modification 19 October 2004/Accepted 23 February 2005

In contrast to previous steady-state analyses of the O2-responsive transcriptome, here we examined the dynamics of the response to short-term anaerobiosis (2 generations) in both catabolite-repressed (glucose) and derepressed (galactose) cells, assessed the specific role that Msn2 and Msn4 play in mediating the response, and identified gene networks using a novel clustering approach. Upon shifting cells to anaerobic conditions in galactose medium, there was an acute (⬃10 min) yet transient (