Science, Engineering and Technology

International Eurasian Conference on

Science, Engineering and Technology (EurasianSciEnTech 2018) 22-23 November 2018 Ankara / Turkey

EurasianSciEnTech 2018 w w w. e u r a s a n s c e n t e c h . o r g

International Eurasian Conference on

Science, Engineering and Technology (EurasianSciEnTech 2018) November 22-23, 2018 / Ankara, Turkey www.EurasianSciEnTech.org

PROCEEDING BOOK

International Eurasian Conference on Science, Engineering and Technology (EurasianSciEnTech 2018), November 22-23, 2018 Ankara, Turkey www.EurasianSciEnTech.org

Chairman of Conference Assoc. Prof. Dr. Muhittin DOĞAN

Gaziantep University, Turkey

General Coordinator Assist. Prof. Dr. Mustafa PEHLİVAN


Organizing Committee Prof. Dr. Ahmet AKSOY

Akdeniz University, Turkey

Prof. Dr. Mehmet Rüştü KARAMAN

Ankara University, Turkey

Prof. Dr. Osman GÜLNAZ

Cukurova University, Turkey

Prof. Dr. Zeliha SELAMOĞLU

Niğde Ömer Halisdemir University, Turkey

International Scientific Committee* Prof. Dr. Adnan QADIR

NED University of Engineering & Technology, Pakistan

Prof. Dr. Ahmet ŞEKEROĞLU

Niğde Ömer Halisdemir University, Turkey

Prof. Dr. Alpaslan DAYANGAÇ

Osmaniye Korkut Ata University, Turkey

Prof. Dr. Ayşe AYDOĞDU

Gazi University, Turkey

Prof. Dr. Azhar RASUL

Government College University, Pakistan

Prof. Dr. Azize AYOL

Dokuz Eylül University, Turkey

Prof. Dr. Bengi USLU


Prof. Dr. Berna DENGİZ

Baskent University, Turkey

Prof. Dr. Bushra MIRZA

Quaid-i-Azam University, Pakistan

Prof. Dr. Bülent AKAY


Prof. Dr. Bülent YEŞİLATA

Harran University, Turkey

Prof. Dr. Cihangir DURAN

Ankara Yıldırım Beyazıt University, Turkey

Prof. Dr. Deniz UZUNSOY

Bursa Technical University, Turkey

Prof. Dr. Dilek ERDİRENÇELEBİ

Konya Technical University, Turkey

Prof. Dr. Ebru ŞENADIM TÜZEMEN

Cumhuriyet University, Turkey

Prof. Dr. Ebru ŞENEL


Prof. Dr. Fakhru'l Razi bin AHMADUN

National Defence University Malaysia, Malaysia

Prof. Dr. Faruk KARADAĞ

Çukurova University, Turkey

Prof. Dr. Fatma Suna BALCI


Prof. Dr. Ferruh ERDOĞDU


Prof. Dr. Gıyasettin KAŞIK

Selçuk University, Turkey

Prof. Dr. Gul Majid KHAN


Prof. Dr. Hüseyin CANBOLAT


Prof. Dr. Kerim KÜÇÜK

Dokuz Eylül University, Turkey

Prof. Dr. Mehmet SARI

Aksaray University, Turkey

Prof. Dr. Meltem SARIOĞLU CEBECİ

Cumhuriyet University, Turkey

Prof. Dr. Muhammad ANSAR


Prof. Dr. Mustafa ÇİĞDEM

Yıldız Technical University, Turkey

Prof. Dr. Neriman BAĞDATLIOĞLU

Celal Bayar University, Turkey

Prof. Dr. Nil TOPLAN

Sakarya University, Turkey

Prof. Dr. Nuri ÖZALP


Prof. Dr. Osman GÜLNAZ


Prof. Dr. Oya BOZDAĞ DÜNDAR


Prof. Dr. Ozan TEKİNALP

Middle East Technical University, Turkey

ii


Prof. Dr. Sarwat JAHAN


Prof. Dr. Sibel YILDIZ

Karadeniz Technical University, Turkey

Prof. Dr. Siti Khairunniza bt. BEJO

Universiti Putra Malaysia, Malaysia

Prof. Dr. Şule UĞUR


Assoc. Prof. Dr. Abd. Rahim Abu TALIB


Assoc. Prof. Dr. Ahmet EKİCİBİL


Assoc. Prof. Dr. Aslıhan DEMİRDÖVEN

Tokat Gaziosmanpasa University, Turkey

Assoc. Prof. Dr. Aycan TOSUNOĞLU

Uludağ University, Turkey

Assoc. Prof. Dr. Ayla UYSAL

Süleyman Demirel University, Turkey

Assoc. Prof. Dr. Ayşe AVCI


Assoc. Prof. Dr. Ayşe BEDELOĞLU

Bursa Technical University, Turkey

Assoc. Prof. Dr. Bülent KABAK

Hitit University, Turkey

Assoc. Prof. Dr. Dian Darina Indah Binti DARUIS


Assoc. Prof. Dr. Didem ÖZÇİMEN

Yıldız Technical University, Turkey

Assoc. Prof. Dr. Fatma MEYDANERİ TEZEL

Karabuk University, Turkey

Assoc. Prof. Dr. Mahmoud HAFEZİEH

Iranian Fisheries Sciences Research Institute, Iran

Assoc. Prof. Dr. Mohammad SUDAGAR

Gorgan University, Iran

Assoc. Prof. Dr. Muhammad Mohsin AMMAN

NED University of Engineering & Technology, Pakistan

Assoc. Prof. Dr. Nemanja KAŠIKOVIĆ

University of Novi Sad, Serbia

Assoc. Prof. Dr. Niyazi ŞAHİN


Assoc. Prof. Dr. Rani Faryal Faheem TAHIR


Assoc. Prof. Dr. Roselina ARELHI

University of Nottingham, Malaysia

Assoc. Prof. Dr. Sadaf ZAIDI

Aligarh Muslim University, India

Assoc. Prof. Dr. Selma DÜZYOL


Assoc. Prof. Dr. Sezgin AYDIN


Assoc. Prof. Dr. Tayfun DEDE

Karadeniz Technical University, Turkey

Assoc. Prof. Dr. Tevfik AĞAÇAYAK


Assist. Prof. Dr. Ali Osman AYAŞ

Adıyaman University, Turkey

Assist. Prof. Dr. Bilge İMER

Middle East Technical University, Turkey

Assist. Prof. Dr. Ebru SARALOĞLU GÜLER

Başkent University, Turkey

Assist. Prof. Dr. Halil İbrahim DEMİR


Assist. Prof. Dr. İbrahim ÇINAR

Karamanoğlu Mehmetbey University, Turkey

Assist. Prof. Dr. Mustafa AKYOL

Adana Science and Technology University, Turkey

Assist. Prof. Dr. Özge TUFAN ÇETİN


Assist. Prof. Dr. Poonam AGARWAL

Jawaharlal Nehru University, India

Assist. Prof. Dr. Shahram DADGAR

Iranian Fisheries Sciences Research Institute, Iran

Dr. Aainaa Izyan NAFSUN


Dr. Lim Kar SING

Universiti Malaysia Pahang, Malaysia

Dr. Tugay AYAŞAN

East Mediterranean Agricultural Research Institute, Turkey

*This list is arranged in alphabetical order. Conference Secretariats Assist. Prof. Dr. Demet DOĞAN


Dr. Mustafa SEVİNDİK


Dr. Celal BAL


Serap ŞAHİN YİĞİT


iii


CONTENTS Page 1. INVAITED SPEAKERS

1

2. FULL-TEXT PAPERS

8

2.1. ORAL PRESENTATIONS 2.2. POSTER PRESENTATIONS

8 2689

iv


Using of Lichens in Pharmaceutical Applications and Traditional Medicine Zeliha Selamoglu Department of Medical Biology, Faculty of Medicine, University of Nigde Ömer Halisdemir, Nigde, Turkey Corresponding author e-mail: [email protected] Abstract Lichens are symbiotic associations formed by mushrooms together algae or cyanobacteria. The studies on lichens due to the difficulties encountered in artificial cultivation are still a few nowadays. Lichens contain bioactive compounds with pharmacological potential in their structure, same as plants and mushrooms. Lichens are the source of many compounds rich in active substances with different biological activities such as antibiotics, antifungal, antiviral, anti-inflammatory, analgesic, antipyretic, antimutagenic, antiproliferative, antitumor, allergic and cytotoxic effects. In this context, information about the pharmacological potential of lichens is given in this work. Keywords: Lichens, Alternative medicine, Medicinal lichens INTRODUCTION Lichens are symbiotic associations formed by mushrooms and cyanobacteria and /or algae. Lichens are known for their powerful medicinal properties as well as for many environmental roles. Lichens are association between fungi (mycobionts) and photoautotrophic, algal partners (photobionts). Since the mycobiont is unique in the symbiotic association and usually dominates the association, lichens are traditionally classified as a life form of fungi. Interactions between the symbiotic partners allow lichens to live in unusual environments. Lichens are able to survive in extreme ecological conditions; they can adapt to extreme temperatures, drought, inundation, salinity, high concentrations of air pollutants and nutrient-poor, highly nitrified environments. Despite this extreme range of ecological adaptations, most lichens are sensitive to changes of their preferred ecological conditions and can hardly grow in nonnative habitats (Demoulin, 1974; Sanders, 2001; Munzi et al., 2014; Spribille et al., 2016; Muggia and Grube, 2018). Lichens are complex symbiotic associations between fungi and algae which are important constituents of many ecosystems. Mushrooms contain many bio-active compounds in their structures (Bal et al., 2017; Sevindik, 2018; Sevindik et al., 2018). Lichens, like mushroom, have many bio-active compounds that are unique in their symbiotic association. The production of various unique extracellular secondary metabolites known as lichen substances is the result of this symbiosis. These compounds exist within the thalli and typically form crystals on the surface of the fungal hyphae. Thus, many secondary metabolites of lichens have been discovered, most of them being exclusively present in lichens. Nowadays, lichens have been taken up for many researches concerning the phytochemical and pharmaceutical applications. Lichens and their secondary metabolites have many pharmaceutical activities, especially including antimicrobial, antioxidant, antiviral, anticancer, antigenotoxic, anti-inflammatory, analgesic and antipyretic properties (Potts et al., 1987; Okuyama et al., 1995; Bugni et al., 2009; Türkez et al., 2010; Mitrović et al., 2011; Shrestha and Clair, 2013; Vu et al., 2015; Cardile et al., 2017; Kumar and Mohandas, 2017; Millot et al., 2017; Nagao et al., 2017; Korkmaz et al., 2018). ORAL PRESENTATION

2383


Lichens have effective interactions with their environment. Production of secondary metabolites in lichens is complex and differently influenced by environmental factors, including light, UV exposure, elevation, temperature and seasonality. Secondary metabolites are not completely essential for the survival and growth of lichens, and the functions of these components in the lichen symbioses are still poorly understood. But, it is important that they may help to protect the thalli against herbivores, pathogens, competitors and external abiotic factors, such as high UV irradiation (Demoulin, 1974; Sanders, 2001; Munzi et al., 2014; Spribille et al., 2016; Muggia and Grube, 2018). Lichens are used for various medicinal purposes, but there are many general categories of use that reoccur across the world. Lichens in traditional medicine are mostly used to treat wounds, skin problems, respiratory and digestive disorders, and obstetric and gynecological case. Mostly the traditional medicinal uses of lichens are probably related to their secondary metabolites, many of which are known to both be physiologically activev and to act as antibiotics. Moreover, some of the traditional uses of lichens also rely on the qualities of lichen carbohydrates. They have been used about both their secondary metabolites and their storage carbohydrates. Lichens are often ate as a decoction to treat ailments relating to either the lungs or the digestive system. Some other uses of lichens are related to obstetrics or treating gynecological problems (Richardson ve Young, 1977; Nash, 1996; Reddy, 2001; Ranković and Kosanić 2015). CONCLUSION Lichen is a dual organism consisting of photosynthetic partner (algae) and a mycobiont (fungi). The optimal physiological circumtances for both the bionts in symbiosis is still unknown. This condition may create an ultimate advantage to provide a continuous, reliable source of natural products from these complex organisms. In the future, metabolic and biotechnological approaches can be used as an alternative production to overcome the limited availability of biologically active, commercially valuable and medicinally important secondary metabolite components.

REFERENCES Bal C, Akgul H, Sevindik M, Akata I, Yumrutas O 2017. Determination of the anti-oxidative activities of six mushrooms. Fresenius Envir Bull, 26: 6246-6252. Bugni TS, Andjelic CD, Pole AR, Rai P, Ireland CM, Barrows LR 2009. Biologically active components of a Papua New Guinea analgesic and anti-inflammatory lichen preparation. Fitoterapia, 80(5): 270273. Cardile V, Graziano ACE, Avola R, Piovano M, Russo A 2017. Potential anticancer activity of lichen secondary metabolite physodic acid. Chemico-biological interactions, 263: 36-45. Demoulin V 1974. The origin of Ascomycetes and Basidiomycetes the case for a red algal ancestry. The Botanical Review, 40(3): 315-346. Kumar SN, Mohandas C 2017. An antifungal mechanism of protolichesterinic acid from the lichen Usnea albopunctata lies in the accumulation of intracellular ROS and mitochondria-mediated cell death due to apoptosis in Candida tropicalis. Frontiers in pharmacology, 8. Korkmaz AI, Akgul H, Sevindik M, Selamoglu Z 2018. Study on determination of bioactive potentials of certain lichens. Acta Alimentaria, 47(1): 80-87. Millot M, Girardot M, Dutreix L, Mambu L, Imbert C 2017. Antifungal and anti-biofilm activities of acetone lichen extracts against Candida albicans. Molecules, 22(4): 651.

ORAL PRESENTATION

2384


Mitrović T, Stamenković S, Cvetković V, Tošić S, Stanković M, Radojević I, Marković S 2011. Antioxidant, antimicrobial and antiproliferative activities of five lichen species. International Journal of Molecular Sciences, 12(8): 5428-5448. Muggia L, Grube M 2018. Fungal diversity in lichens: From extremotolerance to interactions with algae. Life, 8(2): 15. Munzi S, Correia O, Silva P, Lopes N, Freitas C, Branquinho C, Pinho P 2014. Lichens as ecological indicators in urban areas: beyond the effects of pollutants. Journal of applied ecology, 51(6): 17501757. Nagao Y, Nakasone K, Maeshiro T, Nishida N, Kimura K, Kawahigashi Y, Sata M 2017. Successful Treatment of Oral Lichen Planus with Direct-Acting Antiviral Agents after Liver Transplantation for Hepatitis C Virus-Associated Hepatocellular Carcinoma. Case reports in gastroenterology, 11(3): 701710. Nash TH (Ed.) 1996. Lichen biology. Cambridge University Press. Okuyama E, Umeyama K, Yamazaki M, Kinoshita Y, Yamamoto Y 1995. Usnic acid and diffractaic acid as analgesic and antipyretic components of Usnea diffracta. Planta Medica, 61(02): 113-115. Potts AJC, Hamburger J, Scully C 1987. The medication of patients with oral lichen planus and the association of nonsteroidal anti-inflammatory drugs with erosive lesions. Oral surgery, oral medicine, oral pathology, 64(5): 541-543. Ranković B, Kosanić M 2015. Lichens as a potential source of bioactive secondary metabolites. In Lichen Secondary Metabolites (pp. 1-26). Springer, Cham. Reddy SM 2001. University botany I:(algae, fungi, bryophyta and pteridophyta) (Vol. 1). New Age International. Richardson DHS, Young CM 1977. Lichens and Vertebrates. In: Lichen Ecology (Seaward, M. R. D. ed.). Academic Press. New York. 121-44. Sanders WB 2001. Lichens: The Interface between Mycology and Plant Morphology: Whereas most other fungi live as an absorptive mycelium inside their food substrate, the lichen fungi construct a plant-like body within which photosynthetic algal symbionts are cultivated. AIBS Bulletin, 51(12): 1025-1035. Sevindik M 2018. Investigation of Antioxidant/Oxidant Status and Antimicrobial Activities of Lentinus tigrinus. Advances in Pharmacological Sciences, 2018. https://doi.org/10.1155/2018/1718025 Sevindik M, Akgul H, Bal C, Selamoglu Z 2018. Phenolic Contents, Oxidant/Antioxidant Potential and Heavy Metal Levels in Cyclocybe cylindracea. Indian Journal of Pharmaceutical Education and Research, 52(3): 437-441. Shrestha G, Clair LLS 2013. Lichens: a promising source of antibiotic and anticancer drugs. Phytochemistry reviews, 12(1): 229-244. Spribille T, Tuovinen V, Resl P, Vanderpool D, Wolinski H, Aime MC, Mayrhofer H 2016. Basidiomycete yeasts in the cortex of ascomycete macrolichens. Science, 353(6298): 488-492. Türkez H, Geyikoglu F, Aslan A, Karagöz Y, Türkez, Ö, Anar M 2010. Antimutagenic effects of lichen Pseudovernia furfuracea (L.) Zoph. extracts against the mutagenicity of aflatoxin B1 in vitro. Toxicology and Industrial Health, 26(9): 625-631. Vu TH, Le Lamer AC, Lalli C, Boustie J, Samson M, Lohézic-Le Dévéhat F, Le Seyec J 2015. Depsides: lichen metabolites active against hepatitis C virus. PloS one, 10(3): e0120405.

ORAL PRESENTATION

2385