antiviral and immune modifying agent [1-4]. Central European Journal of Chemistry. * E-mail: padilha@ibb.unesp.br. 1FMVZ/UNESP - Postgraduate Program in ...
Cent. Eur. J. Chem. • 9(1) • 2011 • 119-125 DOI: 10.2478/s11532-010-0121-0
Central European Journal of Chemistry
Selenium determination in tissue samples of Nile tilapia using ultrasound-assisted extraction Research Article
Fábio A. Silva , Cilene C. F. Padilha , Gustavo Rocha de Castro , Paulo dos Santos Roldan4, Ana R. de Araujo Nogueira5, Paula M. Moraes6, Pedro M. Padilha6,7* 1
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3
1 FMVZ/UNESP - Postgraduate Program in Zootechny, C. Postal 31, 15385-000, Botucatu, SP, Brazil 2 IB/UNESP – Department of Physical and Biophysical, C. Postal 510, 18618-970, Botucatu, SP, Brazil 3 FEIS/UNESP - Department of Physical and Chemistry, C. Postal 31, 15385-000, Ilha Solteira, SP, Brazil
UFAL - Institute of Chemistry and Technology, 57072-970, Maceió, Al, Brazil
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5 GAIA-EMBRAPA PECUÁRIA SUDESTE, C. Postal 339, 13560-970, São Carlos, SP, Brazil 6 IB/UNESP – Department of Chemistry and Biochemistry, C. Postal 510, 18618-970, Botucatu, SP, Brazil
National Institute of Bioanalytical Science and Technology/UNICAMP, C. Postal 6154, 13084-971, Campinas, SP, Brazil 7
Received 12 July 2010; Accepted 29 September 2010
Abstract: This paper proposes a method to determine selenium in samples of fish muscle and liver tissue using ultrasound assisted extraction process, and analysed by graphite furnace atomic absorption spectrometry (GFAAS). The selenium content was extracted by 0.10 M HCl at the optimal extraction conditions which were established as follows: sample mass of 100 mg; granulometry of the sample
